Cloning of cytochrome p450 genes from nicotiana

ABSTRACT

The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.

[0001] The present invention relates to nucleic acid sequences encodingcytochrome p450 enzymes (hereinafter referred to as p450 and p450enzymes) in Nicotiana plants and methods for using those nucleic acidsequences to alter plant phenotypes.

BACKGROUND

[0002] Cytochrome p450s catalyze enzymatic reactions for a diverse rangeof chemically dissimilar substrates that include the oxidative,peroxidative and reductive metabolism of endogenous and xenobioticsubstrates. In plants, p450s participate in biochemical pathways thatinclude the synthesis of plant products such as phenylpropanoids,alkaloids, terpenoids, lipids, cyanogenic glycosides, and glucosinolates(Chappel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 198, 49:311-343).Cytochrome p450s, also known as p450 heme-thiolate proteins, usually actas terminal oxidases in multi-component electron transfer chains, calledp450-containing monooxygenase systems. Specific reactions catalyzedinclude demethylation, hydroxylation, epoxidation, N-oxidation,sulfooxidation, N-, S-, and O-dealkylations, desulfation, deamination,and reduction of azo, nitro, and N-oxide groups.

[0003] The diverse role of Nicotiana plant p450 enzymes has beenimplicated in effecting a variety of plant metabolites such asphenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides,glucosinolates and a host of other chemical entities. During recentyears, it is becoming apparent that some p450 enzymes can impact thecomposition of plant metabolites in plants. For example, it has beenlong desired to improve the flavor and aroma of certain plants byaltering its profile of selected fatty acids through breeding; howeververy little is known about mechanisms involved in controlling the levelsof these leaf constituents. The down regulation of p450 enzymesassociated with the modification of fatty acids may facilitateaccumulation of desired fatty acids that provide more preferred leafphenotypic qualities. The function of p450 enzymes and their broadeningroles in plant constituents is still being discovered. For instance, aspecial class of p450 enzymes was found to catalyze the breakdown offatty acid into volatile C6- and C9-aldehydes and -alcohols that aremajor contributors of “fresh green” odor of fruits and vegetables. Thelevel of other novel targeted p450s may be altered to enhance thequalities of leaf constituents by modifying lipid composition andrelated break down metabolites in Nicotiana leaf. Several of theseconstituents in leaf are affected by senescence that stimulates thematuration of leaf quality properties. Still other reports have shownthat p450s enzymes are play a functional role in altering fatty acidsthat are involved in plant-pathogen interactions and disease resistance.

[0004] In other instances, p450 enzymes have been suggested to beinvolved in alkaloid biosynthesis. Nornicotine is a minor alkaloid foundin Nicotiana tabaceum. It has been postulated that it is produced by thep450 mediated demethylation of nicotine followed by acylation andnitrosation at the N position thereby producing a series ofN-acylnonicotines and N-nitrosonornicotines. N-demethylation, catalyzedby a putative p450 demethylase, is thought to be a primary source ofnornicotine biosyntheses in Nicotiana. While the enzyme is believed tobe microsomal, thus far a nicotine demethylase enzyme has not beensuccessfully purified, nor have the genes involved been isolated.

[0005] Furthermore, it is hypothesized but not proven that the activityof p450 enzymes is genetically controlled and also strongly influencedby environment factors. For example, the demethylation of nicotine inNicotiana is thought to increase substantially when the plants reach amature stage. Furthermore, it is hypothesized yet not proven that thedemethylase gene contains a transposable element that can inhibittranslation of RNA when present.

[0006] The large multiplicity of p450 enzyme forms, their differingstructure and function have made their research on Nicotiana p450enzymes very difficult before the enclosed invention. In addition,cloning of p450 enzymes has been hampered at least in part because thesemembrane-localized proteins are typically present in low abundance andoften unstable to purification. Hence, a need exists for theidentification of p450 enzymes in plants and the nucleic acid sequencesassociated with those p450 enzymes. In particular, only a few cytochromep450 proteins have been reported in Nicotiana. The inventions describedherein entail the discovery of a substantial number of cytochrome p450fragments that correspond to several groups of p450 species based ontheir sequence identity.

SUMMARY

[0007] The present invention is directed to plant p450 enzymes. Thepresent invention is further directed to plant p450 enzymes fromNicotiana. The present invention is also directed to p450 enzymes inplants whose expression is induced by ethylene and/or plant senescence.The present invention is yet further directed to nucleic acid sequencesin plants having enzymatic activities, for example, being categorized asoxygenase, demethylase and the like, or other and the use of thosesequences to reduce or silence the expression or over-expression ofthese enzymes. The invention also relates to p450 enzymes found inplants containing higher nornicotine levels than plants exhibiting lowernornicotine levels.

[0008] In one aspect, the invention is directed to nucleic acidsequences as set forth in SEQ. ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17,19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53,55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89,91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121,123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151,153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179,181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207,209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235,237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263,265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291,293, 295 and 297.

[0009] In a second related aspect, those fragments containing greaterthan 75% identity in nucleic acid sequence were placed into groupsdependent upon their identity in a region corresponding to the firstnucleic acid following the cytochrome p450 motif GXRXCX(G/A) to the stopcodon. The representative nucleic acid groups and respective species areshown in Table I.

[0010] In a third aspect, the invention is directed to amino acidsequences as set forth in SEQ. ID. Nos. 2, 4, 6, 8, 10, 12, 14, 16, 18,20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54,56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90,92, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122,124, 126, 128, 130, 132, 134, 136, 138, 140, 144, 146, 148, 150, 152,154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180,182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208,210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236,238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264,266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292,294, 296 and 298.

[0011] In a fourth related aspect, those fragments containing greaterthan 71% identity in amino acid sequence were placed into groupsdependent upon their identity to each other in a region corresponding tothe first amino acid following the cytochrome p450 motif GXRXCX(G/A) tothe stop codon. The representative amino acid groups and respectivespecies are shown in Table II.

[0012] In a fifth aspect, the invention is directed to amino acidsequences of full length genes as set forth in SEQ. ID. Nos. 150, 152,154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180,182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208,210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236,238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264,266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292,294, 296 and 298.

[0013] In a sixth related aspect, those full length genes containing 85%or greater identity in amino acid sequence were placed into groupsdependent upon the identity to each other. The representative amino acidgroups and respective species are shown in Table III.

[0014] In a seventh aspect, the invention is directed to amino acidsequences of the fragments set forth in SEQ. ID. Nos. 299-357.

[0015] In the eighth related aspect, those fragments containing 90% orgreater identity in amino acid sequence were placed into groupsdependent upon their identity to each other in a region corresponding tothe first cytochrome p450 domain, UXXRXXZ, to the third cytochromedomain, GXRXO, where U is E or K, X is any amino acid and Z is R, T, Sor M. The representative amino acid groups respective species shown inTable IV.

[0016] In a ninth related aspect, the reduction or elimination orover-expression of p450 enzymes in Nicotiana plants may be accomplishedtransiently using RNA viral systems.

[0017] Resulting transformed or infected plants are assessed forphenotypic changes including, but not limited to, analysis of endogenousp450 RNA transcripts, p450 expressed peptides, and concentrations ofplant metabolites using techniques commonly available to one havingordinary skill in the art.

[0018] In a tenth important aspect, the present invention is alsodirected to generation of trangenic Nicotiana lines that have alteredp450 enzyme activity levels. In accordance with the invention, thesetransgenic lines include nucleic acid sequences that are effective forreducing or silencing or increasing the expression of certain enzymethus resulting in phenotypic effects within Nicotiana. Such nucleic acidsequences include SEQ. ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21,23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57,59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95,97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125,127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155,157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183,185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211,213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239,241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267,269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and297.

[0019] In a very important eleventh aspect of the invention, plantcultivars including nucleic acids of the present invention in a downregulation capacity using either full length genes or fragments thereofor in an over-expression capacity using full length genes will havealtered metabolite profiles relative to control plants.

[0020] In a twelfth aspect of the invention, plant cultivars includingnucleic acid of the present invention using either full length genes orfragments thereof in modifying the biosynthesis or breakdown ofmetabolites derived from the plant or external to the plants, will haveuse in tolerating certain exogenous chemicals or plant pests. Suchnucleic acid sequences include SEQ ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15,17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51,53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87,89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119,121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149,151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177,179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205,207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233,235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261,263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289,291, 293, 295 and 297.

[0021] In a thirteenth aspect, the present invention is directed to thescreening of plants, more preferably Nicotiana, that contain genes thathave substantial nucleic acid identity to the taught nucleic acidsequence. The use of the invention would be advantageous to identify andselect plants that contain a nucleic acid sequence with exact orsubstantial identity where such plants are part of a breeding programfor traditional or transgenic varieties, a mutagenesis program, ornaturally occurring diverse plant populations. The screening of plantsfor substantial nucleic acid identity may be accomplished by evaluatingplant nucleic acid materials using a nucleic acid probe in conjunctionwith nucleic acid detection protocols including, but not limited to,nucleic acid hybridization and PCR analysis. The nucleic acid probe mayconsist of the taught nucleic acid sequence or fragment thereofcorresponding to SEQ ID 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25,27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61,63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99,101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127,129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157,159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185,187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213,215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241,243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269,271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297.

[0022] In a fourteenth aspect, the present invention is directed to theidentification of plant genes, more preferably Nicotiana, that sharesubstantial amino acid identity corresponding to the taught nucleic acidsequence. The identification of plant genes including both cDNA andgenomic clones, those cDNAs and genomic clones, more preferably fromNicotiana may be accomplished by screening plant cDNA libraries using anucleic acid probe in conjunction with nucleic acid detection protocolsincluding, but not limited to, nucleic acid hybridization and PCRanalysis. The nucleic acid probe may be comprised of nucleic acidsequence or fragment thereof corresponding to SEQ ID 1, 3, 5, 7, 9, 11,13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47,49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83,85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117,119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145 and 147.

[0023] In an alterative fifteenth aspect, cDNA expression libraries thatexpress peptides may be screened using antibodies directed to part orall of the taught amino acid sequence. Such amino acid sequences includeSEQ ID 2, 4, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34,36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70,72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 96, 98, 100, 102, 104, 106,108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134,136, 138, 140, 144, 146, 148.

[0024] In a sixteenth important aspect, the present invention is alsodirected to generation of transgenic Nicotiana lines that haveover-expression of p450 enzyme activity levels. In accordance with theinvention, these transgenic lines include all nucleic acid sequencesencoding the amino acid sequences of full length genes that areeffective for increasing the expression of certain enzyme thus resultingin phenotypic effects within Nicotiana. Such amino acid sequencesinclude SEQ. ID. 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170,172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198,200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226,228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254,256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282,284, 286, 288, 290, 292, 294, 296 and 298.

BRIEF DESCRIPTION OF DRAWINGS

[0025]FIG. 1 shows nucleic acid SEQ. ID. No.:1 and amino acid SEQ. ID.No.:2.

[0026]FIG. 2 shows nucleic acid SEQ. ID. No.:3 and amino acid SEQ. ID.No.:4.

[0027]FIG. 3 shows nucleic acid SEQ. ID. No.:5 and amino acid SEQ. ID.No.:6.

[0028]FIG. 4 shows nucleic acid SEQ. ID. No.:7 and amino acid SEQ. ID.No.:8.

[0029]FIG. 5 shows nucleic acid SEQ. ID. No.:9 and amino acid SEQ. ID.No.:10.

[0030]FIG. 6 shows nucleic acid SEQ. ID. No.:11 and amino acid SEQ. ID.No.:12.

[0031]FIG. 7 shows nucleic acid SEQ. ID. No.:13 and amino acid SEQ. ID.No.:14.

[0032]FIG. 8 shows nucleic acid SEQ. ID. No.:15 and amino acid SEQ. ID.No.:16.

[0033]FIG. 9 shows nucleic acid SEQ. ID. No.:17 and amino acid SEQ. ID.No.:18.

[0034]FIG. 10 shows nucleic acid SEQ. ID. No.:19 and amino acid SEQ. ID.No.:20.

[0035]FIG. 11 shows nucleic acid SEQ. ID. No.:21 and amino acid SEQ. ID.No.:22.

[0036]FIG. 12 shows nucleic acid SEQ. ID. No.:23 and amino acid SEQ. ID.No.:24.

[0037]FIG. 13 shows nucleic acid SEQ. ID. No.:25 and amino acid SEQ. ID.No.:26.

[0038]FIG. 14 shows nucleic acid SEQ. ID. No.:27 and amino acid SEQ. ID.No.:28.

[0039]FIG. 15 shows nucleic acid SEQ. ID. No.:29 and amino acid SEQ. ID.No.:30.

[0040]FIG. 16 shows nucleic acid SEQ. ID. No.:31 and amino acid SEQ. ID.No.:32.

[0041]FIG. 17 shows nucleic acid SEQ. ID. No.:33 and amino acid SEQ. ID.No.:34.

[0042]FIG. 18 shows nucleic acid SEQ. ID. No.:35 and amino acid SEQ. ID.No.:36.

[0043]FIG. 19 shows nucleic acid SEQ. ID. No.:37 and amino acid SEQ. ID.No.:38.

[0044]FIG. 20 shows nucleic acid SEQ. ID. No.:39 and amino acid SEQ. ID.No.:40.

[0045]FIG. 21 shows nucleic acid SEQ. ID. No.:41 and amino acid SEQ. ID.No.:42.

[0046]FIG. 22 shows nucleic acid SEQ. ID. No.:43 and amino acid SEQ. ID.No.:44.

[0047]FIG. 23 shows nucleic acid SEQ. ID. No.:45 and amino acid SEQ. ID.No.:46.

[0048]FIG. 24 shows nucleic acid SEQ. ID. No.:47 and amino acid SEQ. ID.No.:48.

[0049]FIG. 25 shows nucleic acid SEQ. ID. No.:49 and amino acid SEQ. ID.No.:50.

[0050]FIG. 26 shows nucleic acid SEQ. ID. No.:51 and amino acid SEQ. ID.No.:52.

[0051]FIG. 27 shows nucleic acid SEQ. ID. No.:53 and amino acid SEQ. ID.No.:54.

[0052]FIG. 28 shows nucleic acid SEQ. ID. No.:55 and amino acid SEQ. ID.No.:56.

[0053]FIG. 29 shows nucleic acid SEQ. ID. No.:57 and amino acid SEQ. ID.No.:58.

[0054]FIG. 30 shows nucleic acid SEQ. ID. No.:59 and amino acid SEQ. ID.No.:60.

[0055]FIG. 31 shows nucleic acid SEQ. ID. No.:61 and amino acid SEQ. ID.No.:62.

[0056]FIG. 32 shows nucleic acid SEQ. ID. No.:63 and amino acid SEQ. ID.No.:64.

[0057]FIG. 33 shows nucleic acid SEQ. ID. No.:65 and amino acid SEQ. ID.No.:66.

[0058]FIG. 34 shows nucleic acid SEQ. ID. No.:67 and amino acid SEQ. ID.No.:68.

[0059]FIG. 35 shows nucleic acid SEQ. ID. No.:69 and amino acid SEQ. ID.No.:70.

[0060]FIG. 36 shows nucleic acid SEQ. ID. No.:71 and amino acid SEQ. ID.No.:72.

[0061]FIG. 37 shows nucleic acid SEQ. ID. No.:73 and amino acid SEQ. ID.No.:74.

[0062]FIG. 38 shows nucleic acid SEQ. ID. No.:75 and amino acid SEQ. ID.No.:76.

[0063]FIG. 39 shows nucleic acid SEQ. ID. No.:77 and amino acid SEQ. ID.No.:78.

[0064]FIG. 40 shows nucleic acid SEQ. ID. No.:79 and amino acid SEQ. ID.No.:80.

[0065]FIG. 41 shows nucleic acid SEQ. ID. No.:81 and amino acid SEQ. ID.No.:82.

[0066]FIG. 42 shows nucleic acid SEQ. ID. No.:83 and amino acid SEQ. ID.No.:84.

[0067]FIG. 43 shows nucleic acid SEQ. ID. No.:85 and amino acid SEQ. ID.No.:86.

[0068]FIG. 44 shows nucleic acid SEQ. ID. No.:87 and amino acid SEQ. ID.No.:88.

[0069]FIG. 45 shows nucleic acid SEQ. ID. No.:89 and amino acid SEQ. ID.No.:90.

[0070]FIG. 46 shows nucleic acid SEQ. ID. No.:91 and amino acid SEQ. ID.No.:92.

[0071]FIG. 48 shows nucleic acid SEQ. ID. No.:95 and amino acid SEQ. ID.No.:96.

[0072]FIG. 49 shows nucleic acid SEQ. ID. No.:97 and amino acid SEQ. ID.No.:98.

[0073]FIG. 50 shows nucleic acid SEQ. ID. No.:99 and amino acid SEQ. ID.No.:100.

[0074]FIG. 51 shows nucleic acid SEQ. ID. No.:101 and amino acid SEQ.ID. No.:102.

[0075]FIG. 52 shows nucleic acid SEQ. ID. No.:103 and amino acid SEQ.ID. No.:104.

[0076]FIG. 53 shows nucleic acid SEQ. ID. No.:105 and amino acid SEQ.ID. No.:106.

[0077]FIG. 54 shows nucleic acid SEQ. ID. No.:107 and amino acid SEQ.ID. No.:108.

[0078]FIG. 55 shows nucleic acid SEQ. ID. No.:109 and amino acid SEQ.ID. No.:110.

[0079]FIG. 56 shows nucleic acid SEQ. ID. No.:111 and amino acid SEQ.ID. No.:112.

[0080]FIG. 57 shows nucleic acid SEQ. ID. No.:113 and amino acid SEQ.ID. No.:114.

[0081]FIG. 58 shows nucleic acid SEQ. ID. No.:115 and amino acid SEQ.ID. No.:116.

[0082]FIG. 59 shows nucleic acid SEQ. ID. No.:117 and amino acid SEQ.ID. No.:118.

[0083]FIG. 60 shows nucleic acid SEQ. ID. No.:119 and amino acid SEQ.ID. No.:120.

[0084]FIG. 61 shows nucleic acid SEQ. ID. No.:121 and amino acid SEQ.ID. No.:122.

[0085]FIG. 62 shows nucleic acid SEQ. ID. No.:123 and amino acid SEQ.ID. No.:124.

[0086]FIG. 63 shows nucleic acid SEQ. ID. No.:125 and amino acid SEQ.ID. No.:126.

[0087]FIG. 64 shows nucleic acid SEQ. ID. No.:127 and amino acid SEQ.ID. No.:128.

[0088]FIG. 65 shows nucleic acid SEQ. ID. No.:129 and amino acid SEQ.ID. No.:130.

[0089]FIG. 66 shows nucleic acid SEQ. ID. No.:131 and amino acid SEQ.ID. No.:132.

[0090]FIG. 67 shows nucleic acid SEQ. ID. No.:133 and amino acid SEQ.ID. No.:134.

[0091]FIG. 68 shows nucleic acid SEQ. ID. No.:135 and amino acid SEQ.ID. No.:136.

[0092]FIG. 69 shows nucleic acid SEQ. ID. No.:137 and amino acid SEQ.ID. No.:138.

[0093]FIG. 70 shows nucleic acid SEQ. ID. No.:139 and amino acid SEQ.ID. No.:140.

[0094]FIG. 72 shows nucleic acid SEQ. ID. No.:143 and amino acid SEQ.ID. No.:144.

[0095]FIG. 73 shows nucleic acid SEQ. ID. No.:145 and amino acid SEQ.ID. No.:146.

[0096]FIG. 74 shows nucleic acid SEQ. ID. No.:147 and amino acid SEQ.ID. No.:148.

[0097]FIG. 75 shows nucleic acid SEQ. ID No.: 149 and amino acid SEQ.ID. No.: 150.

[0098]FIG. 76 shows nucleic acid SEQ. ID No.: 151 and amino acid SEQ.ID. No.: 152.

[0099]FIG. 77 shows nucleic acid SEQ. ID No.: 153 and amino acid SEQ.ID. No.: 154.

[0100]FIG. 78 shows nucleic acid SEQ. ID No.: 155 and amino acid SEQ.ID. No.: 156.

[0101]FIG. 79 shows nucleic acid SEQ. ID No.: 157 and amino acid SEQ.ID. No.: 158.

[0102]FIG. 80 shows nucleic acid SEQ. ID No.: 159 and amino acid SEQ.ID. No.: 160.

[0103]FIG. 81 shows nucleic acid SEQ. ID No.: 161 and amino acid SEQ.ID. No.: 162.

[0104]FIG. 82 shows nucleic acid SEQ. ID No.: 163 and amino acid SEQ.ID. No.: 164.

[0105]FIG. 83 shows nucleic acid SEQ. ID No.: 165 and amino acid SEQ.ID. No.: 166.

[0106]FIG. 84 shows nucleic acid SEQ. ID No.: 167 and amino acid SEQ.ID. No.: 168.

[0107]FIG. 85 shows nucleic acid SEQ. ID No.: 169 and amino acid SEQ.ID. No.: 170.

[0108]FIG. 86 shows nucleic acid SEQ. ID No.: 171 and amino acid SEQ.ID. No.: 172.

[0109]FIG. 87 shows nucleic acid SEQ. ID No.: 173 and amino acid SEQ.ID. No.: 174.

[0110]FIG. 88 shows nucleic acid SEQ. ID No.: 175 and amino acid SEQ.ID. No.: 176.

[0111]FIG. 89 shows nucleic acid SEQ. ID No.: 177 and amino acid SEQ.ID. No.: 178.

[0112]FIG. 90 shows nucleic acid SEQ. ID No.: 179 and amino acid SEQ.ID. No.: 180.

[0113]FIG. 91 shows nucleic acid SEQ. ID No.: 181 and amino acid SEQ.ID. No.: 182.

[0114]FIG. 92 shows nucleic acid SEQ. ID No.: 183 and amino acid SEQ.ID. No.: 184.

[0115]FIG. 93 shows nucleic acid SEQ. ID No.: 185 and amino acid SEQ.ID. No.: 186.

[0116]FIG. 94 shows nucleic acid SEQ. ID No.: 187 and amino acid SEQ.ID. No.: 188.

[0117]FIG. 95 shows nucleic acid SEQ. ID No.: 189 and amino acid SEQ.ID. No.: 190.

[0118]FIG. 96 shows nucleic acid SEQ. ID No.: 191 and amino acid SEQ.ID. No.: 192.

[0119]FIG. 97 shows nucleic acid SEQ. ID No.: 193 and amino acid SEQ.ID. No.: 194.

[0120]FIG. 98 shows nucleic acid SEQ. ID No.: 195 and amino acid SEQ.ID. No.: 196.

[0121]FIG. 99 shows nucleic acid SEQ. ID No.: 197 and amino acid SEQ.ID. No.: 198.

[0122]FIG. 100 shows nucleic acid SEQ. ID No.: 199 and amino acid SEQ.ID. No.: 200.

[0123]FIG. 101 shows nucleic acid SEQ. ID No.: 201 and amino acid SEQ.ID. No.: 202.

[0124]FIG. 102 shows nucleic acid SEQ. ID No.: 203 and amino acid SEQ.ID. No.: 204.

[0125]FIG. 103 shows nucleic acid SEQ. ID No.: 205 and amino acid SEQ.ID. No.: 206.

[0126]FIG. 104 shows nucleic acid SEQ. ID No.: 207 and amino acid SEQ.ID. No.: 208.

[0127]FIG. 105 shows nucleic acid SEQ. ID No.: 209 and amino acid SEQ.ID. No.: 210.

[0128]FIG. 106 shows nucleic acid SEQ. ID No.: 211 and amino acid SEQ.ID. No.: 212.

[0129]FIG. 107 shows nucleic acid SEQ. ID No.: 213 and amino acid SEQ.ID. No.: 214.

[0130]FIG. 108 shows nucleic acid SEQ. ID No.: 215 and amino acid SEQ.ID. No.: 216.

[0131]FIG. 109 shows nucleic acid SEQ. ID No.: 217 and amino acid SEQ.ID. No.: 218.

[0132]FIG. 110 shows nucleic acid SEQ. ID No.: 219 and amino acid SEQ.ID. No.: 220.

[0133]FIG. 111 shows nucleic acid SEQ. ID No.: 221 and amino acid SEQ.ID. No.: 222.

[0134]FIG. 112 shows nucleic acid SEQ. ID No.: 223 and amino acid SEQ.ID. No.: 224.

[0135]FIG. 113 shows nucleic acid SEQ. ID No.: 225 and amino acid SEQ.ID. No.: 226.

[0136]FIG. 114 shows nucleic acid SEQ. ID No.: 227 and amino acid SEQ.ID. No.: 228.

[0137]FIG. 115 shows nucleic acid SEQ. ID No.: 229 and amino acid SEQ.ID. No.: 230.

[0138]FIG. 116 shows nucleic acid SEQ. ID No.: 231 and amino acid SEQ.ID. No.: 232.

[0139]FIG. 117 shows nucleic acid SEQ. ID No.: 233 and amino acid SEQ.ID. No.: 234.

[0140]FIG. 118 shows nucleic acid SEQ. ID No.: 235 and amino acid SEQ.ID. No.: 236.

[0141]FIG. 119 shows nucleic acid SEQ. ID No.: 237 and amino acid SEQ.ID. No.: 238.

[0142]FIG. 120 shows nucleic acid SEQ. ID No.: 239 and amino acid SEQ.ID. No.: 240.

[0143]FIG. 121 shows nucleic acid SEQ. ID No.: 241 and amino acid SEQ.ID. No.: 242.

[0144]FIG. 122 shows nucleic acid SEQ. ID No.: 243 and amino acid SEQ.ID. No.: 244.

[0145]FIG. 123 shows nucleic acid SEQ. ID No.: 245 and amino acid SEQ.ID. No.: 246.

[0146]FIG. 124 shows nucleic acid SEQ. ID No.: 247 and amino acid SEQ.ID. No.: 248.

[0147]FIG. 125 shows nucleic acid SEQ. ID No.: 249 and amino acid SEQ.ID. No.: 250.

[0148]FIG. 126 shows nucleic acid SEQ. ID No.: 251 and amino acid SEQ.ID. No.: 252.

[0149]FIG. 127 shows nucleic acid SEQ. ID No.: 253 and amino acid SEQ.ID. No.: 254.

[0150]FIG. 128 shows nucleic acid SEQ. ID No.: 255 and amino acid SEQ.ID. No.: 256.

[0151]FIG. 129 shows nucleic acid SEQ. ID No.: 257 and amino acid SEQ.ID. No.: 258.

[0152]FIG. 130 shows nucleic acid SEQ. ID No.: 259 and amino acid SEQ.ID. No.: 260.

[0153]FIG. 131 shows nucleic acid SEQ. ID No.: 261 and amino acid SEQ.ID. No.: 262.

[0154]FIG. 132 shows nucleic acid SEQ. ID No.: 263 and amino acid SEQ.ID. No.: 264.

[0155]FIG. 133 shows nucleic acid SEQ. ID No.: 265 and amino acid SEQ.ID. No.: 266.

[0156]FIG. 134 shows nucleic acid SEQ. ID No.: 267 and amino acid SEQ.ID. No.: 268.

[0157]FIG. 135 shows nucleic acid SEQ. ID No.: 269 and amino acid SEQ.ID. No.: 270.

[0158]FIG. 136 shows nucleic acid SEQ. ID No.: 271 and amino acid SEQ.ID. No.: 272.

[0159]FIG. 137 shows nucleic acid SEQ. ID No.: 273 and amino acid SEQ.ID. No.: 274.

[0160]FIG. 138 shows nucleic acid SEQ. ID No.: 275 and amino acid SEQ.ID. No.: 276.

[0161]FIG. 139 shows nucleic acid SEQ. ID No.: 277 and amino acid SEQ.ID. No.: 278.

[0162]FIG. 140 shows nucleic acid SEQ. ID No.: 279 and amino acid SEQ.ID. No.: 280.

[0163]FIG. 141 shows nucleic acid SEQ. ID No.: 281 and amino acid SEQ.ID. No.: 282.

[0164]FIG. 142 shows nucleic acid SEQ. ID No.: 283 and amino acid SEQ.ID. No.: 284.

[0165]FIG. 143 shows nucleic acid SEQ. ID No.: 285 and amino acid SEQ.ID. No.: 286.

[0166]FIG. 144 shows nucleic acid SEQ. ID No.: 287 and amino acid SEQ.ID. No.: 288.

[0167]FIG. 145 shows nucleic acid SEQ. ID No.: 289 and amino acid SEQ.ID. No.: 290.

[0168]FIG. 146 shows nucleic acid SEQ. ID No.: 291 and amino acid SEQ.ID. No.: 292.

[0169]FIG. 147 shows nucleic acid SEQ. ID No.: 293 and amino acid SEQ.ID. No.: 294.

[0170]FIG. 148 shows nucleic acid SEQ. ID No.: 295 and amino acid SEQ.ID. No.: 296.

[0171]FIG. 149 shows nucleic acid SEQ. ID No.: 297 and amino acid SEQ.ID. No.: 298.

[0172]FIG. 151 shows a comparison of Sequence Groups.

[0173]FIG. 152 illustrates alignment of full length clones.

[0174]FIG. 153 shows a procedure used for cloning of cytochrome p450cDNA fragments by PCR

DETAILED DESCRIPTION

[0175] Definitions

[0176] Unless defined otherwise, all technical and scientific terms usedherein have the same meaning as commonly understood by one of ordinaryskill in the art to which this invention belongs. Singleton et al.(1994) Dictionary of Microbiology and Molecular Biology, second edition,John Wiley and Sons (New York) provides one of skill with a generaldictionary of many of the terms used in this invention. All patents andpublications referred to herein are incorporated by reference herein.For purposes of the present invention, the following terms are definedbelow.

[0177] “Enzymatic activity” is meant to include demethylation,hydroxylation, epoxidation, N-oxidation, sulfooxidation, N—, S—, andO-dealkylations, desulfation, deamination, and reduction of azo, nitro,and N-oxide groups. The term “nucleic acid” refers to adeoxyribonucleotide or ribonucleotide polymer in either single- ordouble-stranded form, or sense or anti-sense, and unless otherwiselimited, encompasses known analogues of natural nucleotides thathybridize to nucleic acids in a manner similar to naturally occurringnucleotides. Unless otherwise indicated, a particular nucleic acidsequence includes the complementary sequence thereof.

[0178] The terms “operably linked”, “in operable combination”, and “inoperable order” refer to functional linkage between a nucleic acidexpression control sequence (such as a promoter, signal sequence, orarray of transcription factor binding sites) and a second nucleic acidsequence, wherein the expression control sequence affects transcriptionand/or translation of the nucleic acid corresponding to the secondsequence.

[0179] The term “recombinant” when used with reference to a cellindicates that the cell replicates a heterologous nucleic acid,expresses said nucleic acid or expresses a peptide, heterologouspeptide, or protein encoded by a heterologous nucleic acid. Recombinantcells can express genes or gene fragments in either the sense orantisense form that are not found within the native (non-recombinant)form of the cell. Recombinant cells can also express genes that arefound in the native form of the cell, but wherein the genes are modifiedand re-introduced into the cell by artificial means.

[0180] A “structural gene” is that portion of a gene comprising a DNAsegment encoding a protein, polypeptide or a portion thereof, andexcluding the 5′ sequence which drives the initiation of transcription.The structural gene may alternatively encode a nontranslatable product.The structural gene may be one which is normally found in the cell orone which is not normally found in the cell or cellular location whereinit is introduced, in which case it is termed a “heterologous gene”. Aheterologous gene may be derived in whole or in part from any sourceknown to the art, including a bacterial genome or episome, eukaryotic,nuclear or plasmid DNA, cDNA, viral DNA or chemically synthesized DNA. Astructural gene may contain one or more modifications that could effectbiological activity or its characteristics, the biological activity orthe chemical structure of the expression product, the rate of expressionor the manner of expression control. Such modifications include, but arenot limited to, mutations, insertions, deletions and substitutions ofone or more nucleotides. The structural gene may constitute anuninterrupted coding sequence or it may include one or more introns,bounded by the appropriate splice junctions. The structural gene may betranslatable or non-translatable, including in an anti-senseorientation. The structural gene may be a composite of segments derivedfrom a plurality of sources and from a plurality of gene sequences(naturally occurring or synthetic, where synthetic refers to DNA that ischemically synthesized).

[0181] “Derived from” is used to mean taken, obtained, received, traced,replicated or descended from a source (chemical and/or biological). Aderivative may be produced by chemical or biological manipulation(including, but not limited to, substitution, addition, insertion,deletion, extraction, isolation, mutation and replication) of theoriginal source.

[0182] “Chemically synthesized”, as related to a sequence of DNA, meansthat portions of the component nucleotides were assembled in vitro.Manual chemical synthesis of DNA may be accomplished using wellestablished procedures (Caruthers, Methodology of DNA and RNASeauencing, (1983), Weissman (ed.), Praeger Publishers, New York,Chapter 1); automated chemical synthesis can be performed using one of anumber of commercially available machines.

[0183] Optimal alignment of sequences for comparison may be conducted bythe local homology algorithm of Smith and Waterman, Adv. Appl. Math.2:482 (1981), by the homology alignment algorithm of Needleman andWunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity methodof Pearson and Lipman Proc. Natl. Acad. Sci. (U.S.A.) 85: 2444 (1988),by computerized implementations of these algorithms (GAP, BESTFIT,FASTA, and TFASTA in the Wisconsin Genetics Software Package, GeneticsComputer Group, 575 Science Dr., Madison, Wis.), or by inspection.

[0184] The NCBI Basic Local Alignment Search Tool (BLAST) (Altschul etal., 1990) is available from several sources, including the NationalCenter for Biological Information (NCBI, Bethesda, Md.) and on theInternet, for use in connection with the sequence analysis programsblastp, blastn, blastx, tblastn and tblastx. It can be accessed athtp://www.ncbi.nlm.nih.gov/BLAST/. A description of how to determinesequence identity using this program is available athttp://www.ncbi.nlm.nih.gov/BLAST/blast help.html.

[0185] The terms “substantial amino acid identity” or “substantial aminoacid sequence identity” as applied to amino acid sequences and as usedherein denote a characteristic of a polypeptide, wherein the peptidecomprises a sequence that has at least 70 percent sequence identity,preferably 80 percent amino acid sequence identity, more preferably 90percent amino acid sequence identity, and most preferably at least 99 to100 percent sequence identity as compared to a reference group overregion corresponding to the first amino acid following the cytochromep450 motif GXRXCX(G/A) to the stop codon of the translated peptide.

[0186] The terms “substantial nucleic acid identity” or “substantialnucleic acid sequence identity” as applied to nucleic acid sequences andas used herein denote a characteristic of a polynucleotide sequence,wherein the polynucleotide comprises a sequence that has at least 75percent sequence identity, preferably 81 percent amino acid sequenceidentity, more preferably at least 91 percent sequence identity, andmost preferably at least 99 to 100 percent sequence identity as comparedto a reference group over region corresponding to the first nucleic acidfollowing the cytochrome p450 motif GXRXCX(G/A) to the stop codon of thetranslated peptide.

[0187] Another indication that nucleotide sequences are substantiallyidentical is if two molecules hybridize to each other under stringentconditions. Stringent conditions are sequence-dependent and will bedifferent in different circumstances. Generally, stringent conditionsare selected to be about 5° C. to about 20° C., usually about 10° C. toabout 15° C., lower than the thermal melting point (Tm) for the specificsequence at a defined ionic strength and pH. The Tm is the temperature(under defined ionic strength and pH) at which 50% of the targetsequence hybridizes to a matched probe. Typically, stringent conditionswill be those in which the salt concentration is about 0.02 molar at pH7 and the temperature is at least about 60° C. For instance in astandard Southern hybridization procedure, stringent conditions willinclude an initial wash in 6×SSC at 42° C. followed by one or moreadditional washes in 0.2×SSC at a temperature of at least about 55° C.,typically about 60° C. and often about 65° C.

[0188] Nucleotide sequences are also substantially identical forpurposes of this invention when the polypeptides and/or proteins whichthey encode are substantially identical. Thus, where one nucleic acidsequence encodes essentially the same polypeptide as a second nucleicacid sequence, the two nucleic acid sequences are substantiallyidentical, even if they would not hybridize under stringent conditionsdue to degeneracy permitted by the genetic code (see, Darnell et al.(1990) Molecular Cell Biology, Second Edition Scientific American BooksW. H. Freeman and Company New York for an explanation of codondegeneracy and the genetic code). Protein purity or homogeneity can beindicated by a number of means well known in the art, such aspolyacrylamide gel electrophoresis of a protein sample, followed byvisualization upon staining. For certain purposes high resolution may beneeded and HPLC or a similar means for purification may be utilized.

[0189] As used herein, the term “vector” is used in reference to nucleicacid molecules that transfer DNA segment(s) into a cell. A vector mayact to replicate DNA and may reproduce independently in a host cell. Theterm “vehicle” is sometimes used interchangeably with “vector.” The term“expression vector” as used herein refers to a recombinant DNA moleculecontaining a desired coding sequence and appropriate nucleic acidsequences necessary for the expression of the operably linked codingsequence in a particular host organism. Nucleic acid sequences necessaryfor expression in prokaryotes usually include a promoter, an operator(optional), and a ribosome binding site, often along with othersequences. Eucaryotic cells are known to utilize promoters, enhancers,and termination and polyadenylation signals.

[0190] For the purpose of regenerating complete genetically engineeredplants with roots, a nucleic acid may be inserted into plant cells, forexample, by any technique such as in vivo inoculation or by any of theknown in vitro tissue culture techniques to produce transformed plantcells that can be regenerated into complete plants. Thus, for example,the insertion into plant cells may be by in vitro inoculation bypathogenic or non-pathogenic A. tumefaciens. Other such tissue culturetechniques may also be employed.

[0191] “Plant tissue” includes differentiated and undifferentiatedtissues of plants, including, but not limited to, roots, shoots, leaves,pollen, seeds, tumor tissue and various forms of cells in culture, suchas single cells, protoplasts, embryos and callus tissue. The planttissue may be in planta or in organ, tissue or cell culture.

[0192] “Plant cell” as used herein includes plant cells in planta andplant cells and protoplasts in culture.

[0193] “cDNA” or “complementary DNA” generally refers to a singlestranded DNA molecule with a nucleotide sequence that is complementaryto an RNA molecule. cDNA is formed by the action of the enzyme reversetranscriptase on an RNA template.

[0194] Strategies for Obtaining Nucleic Acid Sequences

[0195] In accordance with the present invention, RNA was extracted fromNicotiana tissue of converter and non-converter Nicotiana lines. Theextracted RNA was then used to create cDNA. Nucleic acid sequences ofthe present invention were then generated using two strategies.

[0196] In the first strategy, the poly A enriched RNA was extracted fromplant tissue and cDNA was made by reverse transcription PCR. The singlestrand cDNA was then used to create p450 specific PCR populations usingdegenerate primers plus a oligo d(T) reverse primer. The primer designwas based on the highly conserved motifs of p450. Examples of specificdegenerate primers are set forth in FIG. 1. Sequence fragments fromplasmids containing appropriate size inserts were further analyzed.These size inserts typically ranged from about 300 to about 800nucleotides depending on which primers were used.

[0197] In a second strategy, a cDNA library was initially constructed.The cDNA in the plasmids was used to create p450 specific PCRpopulations using degenerate primers plus T7 primer on plasmid asreverse primer. As in the first strategy, sequence fragments fromplasmids containing appropriate size inserts were further analyzed.

[0198] Nicotiana plant lines known to produce high levels of nornicotine(converter) and plant lines having undetectable levels of nornicotinemay be used as starting materials.

[0199] Leaves can then be removed from plants and treated with ethyleneto activate p450 enzymatic activities defined herein. Total RNA isextracted using techniques known in the art. cDNA fragments can then begenerated using PCR (RT-PCR) with the oligo d(T) primer as described inFIG. 153. The cDNA library can then be constructed more fully describedin examples herein.

[0200] The conserved region of p450 type enzymes can be used as atemplate for degenerate primers (FIG. 75). Using degenerate primers,p450 specific bands can be amplified by PCR. Bands indicative for p450like enzymes can be identified by DNA sequencing. PCR fragments can becharacterized using BLAST search, alignment or other tools to identifyappropriate candidates.

[0201] Sequence information from identified fragments can be used todevelop PCR primers. These primers in combination of plasmid primers incDNA library were used to clone full length p450 genes. Large-scaleSouthern reverse analysis was conducted to examine the differentialexpression for all fragment clones obtained and in some cases fulllength clones. In this aspect of the invention, these large-scalereverse Southern assays can be conducted using labeled total cDNA's fromdifferent tissues as a probe to hybridize with cloned DNA fragments inorder to screen all cloned inserts. Nonradioactive and radioactive (p³²)Northern blotting assays were also used to characterize clones p450fragments and full length clones.

[0202] Peptide specific antibodies were made against several full-lengthclones by deriving their amino acid sequence and selecting peptideregions that were antigenic and unique relative to other clones. Rabbitantibodies were made to synthetic peptides conjugated to a carrierprotein. Western blotting analyses or other immunological methods wereperformed on plant tissue using these antibodies.

[0203] Nucleic acid sequences identified as described above can beexamined by using virus induced gene silencing technology (VIGS,Baulcombe, Current Opinions in Plant Biology, 1999, 2:109-113).

[0204] Peptide specific antibodies were made for several full-lengthclones by deriving their amino acid sequence and selecting peptideregions that were potentially antigenic and were unique relative toother clones. Rabbit antibodies were made to synthetic petidesconjugated to a carrier protein. Western blotting analyses were perfomedusing these antibodies.

[0205] In another aspect of the invention, interfering RNA technology(RNAi) is used to further characterize cytochrome p450 enzymaticactivities in Nicotiana plants of the present invention. The followingreferences which describe this technology are incorporated by referenceherein, Smith et al., Nature, 2000, 407:319-320; Fire et al., Nature,1998, 391:306- 311; Waterhouse et al., PNAS, 1998, 95:13959-13964;Stalberg et al., Plant Molecular Biology, 1993, 23:671- 683; Baulcombe,Current Opinions in Plant Biology, 1999, 2:109-113; and Brigneti et al.,EMBO Journal, 1998, 17(22):6739-6746. Plants may be transformed usingRNAi techniques, antisense techniques, or a variety of other methodsdescribed.

[0206] Several techniques exist for introducing foreign genetic materialinto plant cells, and for obtaining plants that stably maintain andexpress the introduced gene. Such techniques include acceleration ofgenetic material coated onto microparticles directly into cells (U.S.Pat. Nos. 4,945,050 to Cornell and 5,141,131 to DowElanco). Plants maybe transformed using Agrobacterium technology, see U.S. Pat. No.5,177,010 to University of Toledo, U.S. Pat. No. 5,104,310 to Texas A&M,European Patent Application 0131624B1, European Patent Applications120516, 159418B1, European Patent Applications 120516, 159418B1 and176,112 to Schilperoot, U.S. Pat. Nos. 5,149,645, 5,469,976, 5,464,763and 4,940,838 and 4,693,976 to Schilperoot, European Patent Applications116718, 290799, 320500 all to MaxPlanck, European Patent Applications604662 and 627752 to Japan Nicotiana, European Patent Applications0267159, and 0292435 and U.S. Pat. No. 5,231,019 all to Ciba Geigy, U.S.Pat. Nos. 5,463,174 and 4,762,785 both to Calgene, and U.S. Pat. Nos.5,004,863 and 5,159,135 both to Agracetus. Other transformationtechnology includes whiskers technology, see U.S. Pat. Nos. 5,302,523and 5,464,765 both to Zeneca. Electroporation technology has also beenused to transform plants, see WO 87/06614 to Boyce Thompson Institute,U.S. Pat. Nos. 5,472,869 and 5,384,253 both to Dekalb, WO9209696 andWO9321335 both to PGS. All of these transformation patents andpublications are incorporated by reference. In addition to numeroustechnologies for transforming plants, the type of tissue which iscontacted with the foreign genes may vary as well. Such tissue wouldinclude but would not be limited to embryogenic tissue, callus tissuetype I and II, hypocotyl, meristem, and the like. Almost all planttissues may be transformed during dedifferentiation using appropriatetechniques within the skill of an artisan.

[0207] Foreign genetic material introduced into a plant may include aselectable marker. The preference for a particular marker is at thediscretion of the artisan, but any of the following selectable markersmay be used along with any other gene not listed herein which couldfunction as a selectable marker. Such selectable markers include but arenot limited to aminoglycoside phosphotransferase gene of transposon Tn5(Aph II) which encodes resistance to the antibiotics kanamycin, neomycinand G418, as well as those genes which code for resistance or toleranceto glyphosate; hygromycin; methotrexate; phosphinothricin (bar);imidazolinones, sulfonylureas and triazolopyrimidine herbicides, such aschlorosulfuron; bromoxynil, dalapon and the like.

[0208] In addition to a selectable marker, it may be desirous to use areporter gene. In some instances a reporter gene may be used without aselectable marker. Reporter genes are genes which are typically notpresent or expressed in the recipient organism or tissue. The reportergene typically encodes for a protein which provide for some phenotypicchange or enzymatic property. Examples of such genes are provided in K.Weising et al. Ann. Rev. Genetics, 22, 421 (1988), which is incorporatedherein by reference. Preferred reporter genes include without limitationglucuronidase (GUS) gene and GFP genes.

[0209] Once introduced into the plant tissue, the expression of thestructural gene may be assayed by any means known to the art, andexpression may be measured as mRNA transcribed, protein synthesized, orthe amount of gene silencing that occurs (see U.S. Pat. No. 5,583,021which is hereby incorporated by reference). Techniques are known for thein vitro culture of plant tissue, and in a number of cases, forregeneration into whole plants (EP Appln No. 88810309.0). Procedures fortransferring the introduced expression complex to commercially usefulcultivars are known to those skilled in the art.

[0210] Once plant cells expressing the desired level of p450 enzyme areobtained, plant tissues and whole plants can be regenerated therefromusing methods and techniques well-known in the art. The regeneratedplants are then reproduced by conventional means and the introducedgenes can be transferred to other strains and cultivars by conventionalplant breeding techniques.

[0211] The following examples illustrate methods for carrying out theinvention and should be understood to be illustrative of, but notlimiting upon, the scope of the invention which is defined in theappended claims.

EXAMPLES Example 1 Development of Plant Tissue and Ethylene Treatment

[0212] Plant Growth

[0213] Plants were seeded in pots and grown in a greenhouse for 4 weeks.The 4 week old seedlings were transplanted into individual pots andgrown in the greenhouse for 2 months. The plants were watered 2 times aday with water containing 150 ppm NPK fertilizer during growth. Theexpanded green leaves were detached from plants to do the ethylenetreatment described below.

[0214] Cell Line 78379

[0215] Tobacco line 78379, which is a burley tobacco line released bythe University of Kentucky was used as a source of plant material. Onehundred plants were cultured as standard in the art of growing tobaccoand transplanted and tagged with a distinctive number (1-100).Fertilization and field management were conducted as recommended.

[0216] Three quarters of the 100 plants converted between 20 and 100% ofthe nicotine to nornicotine. One quarter of the 100 plants convertedless than 5% of the nicotine to nornicotine. Plant number 87 had theleast conversion (2%) while plant number 21 had 100% conversion. Plantsconverting less than 3% were classified as non-converters.Self-pollinated seed of plant number 87 and plant number 21, as well ascrossed (21×87 and 87×21) seeds were made to study genetic andphenotypic differences. Plants from selfed 21 were converters, and 99%of selfs from 87 were non-converters. The other 1% of the plants from 87showed low conversion (5-15%). Plants from reciprocal crosses were allconverters.

[0217] Cell Line 4407

[0218] Nicotiana line 4407, which is a burley line was used as a sourceof plant material. Uniform and representative plants (100) were selectedand tagged. Of the 100 plants 97 were non-converters and three wereconverters. Plant number 56 had the least amount of conversion (1.2%)and plant number 58 had the highest level of conversion (96%).Self-pollenated seeds and crossed seeds were made with these two plants.

[0219] Plants from selfed-58 segregated with 3:1 converter tonon-converter ratio. Plants 58-33 and 58-25, were identified ashomozygous converter and nonconverter plant lines, respectively. Thestable conversion of 58-33 was confirmed by analysis of its progenies ofnext generation.

[0220] Cell Line PBLB01

[0221] PBLB01 is a burley line developed by ProfiGen, Inc. and was usedas a source of plant material. The converter plant was selected fromfoundation seeds of PBLB01.

[0222] Ethylene Treatment Procedures

[0223] Green leaves were detached from 2-3 month greenhouse grown plantsand sprayed with 0.3% ethylene solution (Prep brand Ethephon(Rhone-Poulenc)). Each sprayed leaf was hung in a curing rack equippedwith humidifier and covered with plastic. During the treatment, thesample leaves were periodically sprayed with the ethylene solution.Approximately 24-48 hour post ethylene treatment, leaves were collectedfor RNA extraction. Another sub-sample was taken for metabolicconstituent analysis to determine the concentration of leaf metabolitesand more specific constituents of interest such as a variety ofalkaloids.

[0224] As an example, alkaloids analysis could be performed as follows.Samples (0.1 g) were shaken at 150 rpm with 0.5 ml 2N NaOH, and a 5 mlextraction solution which contained quinoline as an internal standardand methyl t-butyl ether. Samples were analyzed on a HP 6890 GC equippedwith a FID detector. A temperature of 250° C. was used for the detectorand injector. An HP column (30 m-0.32 nm-1 m) consisting of fused silicacrosslinked with 5% phenol and 95% methyl silicon was used at atemperature gradient of 110-185° C. at 10° C. per minute. The column wasoperated at 100° C. with a flow rate of 1.7 cm³min⁻¹ with a split ratioof 40:1 with a 2 l injection volume using helium as the carrier gas.

Example 2 RNA Isolation

[0225] For RNA extractions, middle leaves from 2 month old greenhousegrown plants were treated with ethylene as described. The 0 and 24-48hours samples were used for RNA extraction. In some cases, leaf samplesunder the senescence process were taken from the plants 10 days postflower-head removal. These samples were also used for extraction. TotalRNA was isolated using Rneasy Plant Mini Kit® (Qiagen, Inc., Valencia,Calif.) following manufacturer's protocol.

[0226] The tissue sample was ground under liquid nitrogen to a finepowder using a DEPC treated mortar and pestle. Approximately 100milligrams of ground tissue were transferred to a sterile 1.5 mleppendorf tube. This sample tube was placed in liquid nitrogen until allsamples were collected. Then, 450μ-l of Buffer RLT as provided in thekit (with the addition of Mercaptoethanol) was added to each individualtube. The sample was vortexed vigorously and incubated at 56° C. for 3minutes. The lysate was then, applied to the QIAshredder™ spin columnsitting in a 2-ml collection tube, and centrifuged for 2 minutes atmaximum speed. The flow through was collected and 0.5 volume of ethanolwas added to the cleared lysate. The sample is mixed well andtransferred to an Rneasy® mini spin column sitting in a 2 ml collectiontube. The sample was centrifuged for 1 minute at 10,000 rpm. Next, 700μl of buffer RW1 was pipetted onto the Rneasy® column and centrifugedfor 1 minute at 10,000 rpm. Buffer RPE was pipetted onto the Rneasy®column in a new collection tube and centrifuged for 1 minute at 10,000rpm. Buffer RPE was again, added to the Rneasy® spin column andcentrifuged for 2 minutes at maximum speed to dry the membrane. Toeliminate any ethanol carry over, the membrane was placed in a separatecollection tube and centrifuged for an additional 1 minute at maximumspeed. The Rneasy® column was transferred into a new 1.5 ml collectiontube, and 40 μl of Rnase-free water was pipetted directly onto theRneasy® membrane. This final elute tube was centrifuged for 1 minute at10,000 rpm. Quality and quantity of total RNA was analyzed by denaturedformaldehyde gel and spectrophotometer.

[0227] Poly(A)RNA was isolated using Oligotex™ poly A+ RNA purificationkit (Qiagen Inc.) following manufacture's protocol. About 200 μg totalRNA in 250 μl maximum volume was used. A volume of 250 μl of Buffer OBBand 15 μl of oligotex™ suspension was added to the 250 μl of total RNA.The contents were mixed thoroughly by pipetting and incubated for 3minutes at 70° C. on a heating block. The sample was then, placed atroom temperature for approximately 20 minutes. The oligotex:mRNA complexwas pelleted by centrifugation for 2 minutes at maximum speed. All but50 μl of the supernatant was removed from the microcentrifuge tube. Thesample was treated further by OBB buffer. The oligotex:mRNA pellet wasresuspended in 400 μl of Buffer OW2 by vortexing. This mix wastransferred onto a small spin column placed in a new tube andcentrifuged for 1 minute at maximum speed. The spin column wastransferred to a new tube and an additional 400 μl of Buffer OW2 wasadded to the column. The tube was then centrifuged for 1 minute atmaximum speed. The spin column was transferred to a final 1.5 mlmicrocentrifuge tube. The sample was eluted with 60 ul of hot (70° C.)Buffer OEB. Poly A product was analyzed by denatured formaldehyde gelsand spectrophotometric analysis.

Example 3 Reverse Transcription-PCR

[0228] First strand cDNA was produced using SuperScript reversetranscriptase following manufacturer's protocol (Invitrogen, Carlsbad,Calif.). The poly A+ enriched RNA/oligo dT primer mix consisted of lessthan 5 μg of total RNA, 1 μl of 10 mM DNTP mix, 1 μl of Oligo d(T)₁₂₋₁₈(0.5 μg/ul), and up to 10 μl of DEPC-treated water. Each sample wasincubated at 65° C. for 5 minutes, then placed on ice for at least 1minute. A reaction mixture was prepared by adding each of the followingcomponents in order: 2 μl 10× RT buffer, 4 μl of 25 mM MgCl2, 2 μl of0.1 M DTT, and 1 μl of RNase OUT Recombinant RNase Inhibitor. Anaddition of 9 μl of reaction mixture was pipetted to each RNA/primermixture and gently mixed. It was incubated at 42° C. for 2 minutes and 1μl of Super Script II™ RT was added to each tube. The tube was incubatedfor 50 minutes at 42° C. The reaction was terminated at 70° C. for 15minutes and chilled on ice. The sample was collected by centrifugationand 1 μl of RNase H was added to each tube and incubated for 20 minutesat 37° C. The second PCR was carried out with 200 pmoles of forwardprimer (degenerate primers as in FIG. 75, SEQ.ID Nos. 149-156) and 100pmoles reverse primer (mix of 18 nt oligo d(T) followed by 1 randombase).

[0229] Reaction conditions were 94° C. for 2 minutes and then performed40 cycles of PCR at 94° C. for 1 minute, 45° to 60° C. for 2 minutes,72° C. for 3 minutes with a 72° C. extension for an extra 10 min.

[0230] Ten microliters of the amplified sample were analyzed byelectrophoresis using a 1% agarose gel. The correct size fragments werepurified from agarose gel.

Example 4 Generation of PCR Fragment Populations

[0231] PCR fragments from Example 3 were ligated into a pGEM-T® EasyVector (Promega, Madison, Wis.) following manufacturer's instructions.The ligated product was transformed into JM109 competent cells andplated on LB media plates for blue/white selection. Colonies wereselected and grown in a 96 well plate with 1.2 ml of LB media overnightat 37° C. Frozen stock was generated for all selected colonies. PlasmidDNA from plates were purified using Beckman's Biomeck 2000 minipreprobotics with Wizard SV Miniprep® kit (Promega). Plasmid DNA was elutedwith 100 μl water and stored in a 96 well plate. Plasmids were digestedby EcoR1 and were analyzed using 1% agarose gel to confirm the DNAquantity and size of inserts. The plasmids containing a 400-600 bpinsert were sequenced using an CEQ 2000 sequencer (Beckman, Fullerton,Calif.). The sequences were aligned with GenBank database by BLASTsearch. The p450 related fragments were identified and further analyzed.Alternatively, p450 fragments were isolated from substraction libraries.These fragments were also analyzed as described above.

Example 5 Construction of CDNA Library

[0232] A cDNA library was constructed by preparing total RNA fromethylene treated leaves as follows. First, total RNA was extracted fromethylene treated leaves of tobacco line 58-33 using a modified acidphenol and chloroform extraction protocol. Protocol was modified to useone gram of tissue that was ground and subsequently vortexed in 5 ml ofextraction buffer (100 mM Tris-HCl, pH 8.5; 200 mM NaCl; 10 mM EDTA;0.5% SDS) to which 5 ml phenol (pHS.5) and 5 ml chloroform was added.The extracted sample was centrifuged and the supernatant was saved. Thisextraction step was repeated 2-3 more times until the supernatantappeared clear. Approximately 5 ml of chloroform was added to removetrace amounts of phenol. RNA was precipitated from the combinedsupernatant fractions by adding a 3-fold volume of ETOH and 1/10 volumeof 3M NaOAc (pH5.2) and storing at −20° C. for 1 hour. Aftertransferring to a Corex glass container the RNA fraction was centrifugedat 9,000 RPM for 45 minutes at 4° C. The pellet was washed with 70%ethanol and spun for 5 minutes at 9,000 RPM at 4° C. After drying thepellet, the pelleted RNA was dissolved in 0.5 ml RNase free water. Thepelleted RNA was dissolved in 0.5 ml RNase free water. The quality andquantity of total RNA was analyzed by denatured formaldehyde gel andspectrophotometer, respectively.

[0233] The resultant total RNA was isolated for poly A+ RNA using anOligo(dT) cellulose protocol (Invitrogen) and Microcentrifuge spincolumns (Invitrogen) by the following protocol. Approximately twenty mgof total RNA was subjected to twice purification to obtain high qualitypoly A+ RNA. Poly A+ RNA product was analyzed by performing denaturedformaldehyde gel and subsequent RT-PCR of known full-length genes toensure high quality of mRNA.

[0234] Next, poly A+ RNA was used as template to produce a CDNA libraryemploying cDNA synthesis kit, ZAP-cDNA® synthesis kit, and ZAP-cDNA®Gigapack® III gold cloning kit (Stratagene, La Jolla, Calif.). Themethod involved following the manufacture's protocol as specified.Approximately 8 μg of poly A+ RNA was used to construct cDNA library.Analysis of the primary library revealed about 2.5×10⁶-1×10⁷ pfu. Aquality background test of the library was completed by complementationassays using IPTG and X-gal, where recombinant plaques was expressed atmore than 100-fold above the background reaction.

[0235] A more quantitative analysis of the library by random PCR showedthat average size of insert cDNA was approximately 1.2 kb. The methodused a two-step PCR method as followed. For the first step, reverseprimers were designed based on the preliminary sequence informationobtained from p450 fragments. The designed reverse primers and T3(forward) primers were used amplify corresponding genes from the cDNAlibrary. PCR reactions were subjected to agarose electrophoresis and thecorresponding bands of high molecular weight were excised, purified,cloned and sequenced. In the second step, new primers designed from5′UTR or the start coding region of p450 as the forward primers togetherwith the reverse primers (designed from 3′UTR of p450) were used in thesubsequent PCR to obtain full-length p450 clones.

[0236] The p450 fragments were generated by PCR amplification from theconstructed cDNA library as described in Example 3 with the exception ofthe reverse primer. The T7 primer located on the plasmid downstream ofcDNA inserts (see FIG. 75) was used as a reverse primer. PCR fragmentswere isolated, cloned and sequenced as described in Example 4.

[0237] Full-length p450 genes were isolated by PCR method fromconstructed cDNA library. Gene specific reverse primers (designed fromthe downstream sequence of p450 fragments) and a forward primer (T3 onlibrary plasmid) were used to clone the full length genes. PCR fragmentswere isolated, cloned and sequenced. If necessary, second step PCR wasapplied. In the second step, new forward primers designed from 5′UTR ofcloned p450s together with the reverse primers designed from 3′UTR ofp450 clones were used in the subsequent PCR reactions to obtainfull-length p450 clones. The clones were subsequently sequenced.

Example 6 Characterization of Cloned Fragments—Reverse Southern BlottingAnalysis

[0238] Nonradioactive large scale reverse southern blotting assays wereperformed on all p450 clones identified in above examples to detect thedifferential expression. It was observed that the level of expressionamong different p450 clusters was very different. Further real timedetection was conducted on those with high expression.

[0239] Nonradioactive Southern blotting procedures were conducted asfollows.

[0240] 1) Total RNA was extracted from ethylene treated and nontreatedconverter (58-33) and nonconverter (58-25) leaves using the QiagenRnaeasy kit as described in Example 2.

[0241] 2) Probe was produced by biotin-tail labeling a single strandcDNA derived from poly A+ enriched RNA generated in above step. Thislabeled single strand cDNA was generated by RT-PCR of the converter andnonconverter total RNA (Invitrogen) as described in Example 3 with theexception of using biotinalyted oligo dT as a primer (Promega). Thesewere used as a probe to hybridize with cloned DNA.

[0242] 3) Plasmid DNA was digested with restriction enzyme EcoR1 and runon agarose gels. Gels were simultaneously dried and transferred to twonylon membranes (Biodyne B®). One membrane was hybridized with converterprobe and the other with nonconverter probe. Membranes wereUV-crosslinked (auto crosslink setting, 254 nm, Stratagene,Stratalinker) before hybridization.

[0243] Alternatively, the inserts were PCR amplified from each plasmidusing the sequences located on both arms of p-GEM plasmid, T3 and SP6,as primers. The PCR products were analyzed by running on a 96 wellReady-to-run agarose gels. The confirmed inserts were dotted on twonylon membranes. One membrane was hybridized with converter probe andthe other with nonconverter probe.

[0244] 4) The membranes were hybridized and washed followingmanufacture's instruction with the modification of washing stringency(Enzo MaxSence™ kit, Enzo Diagnostics, Inc, Farmingdale, N.Y.). Themembranes were prehybridized with hybridization buffer (2×SSC bufferedformamide, containing detergent and hybridization enhancers) at 42° C.for 30 min and hybridized with 10 μl denatured probe overnight at 42° C.The membranes then were washed in 1× hybridization wash buffer 1 time atroom temperature for 10 min and 4 times at 68° C. for 15 min. Themembranes were ready for the detection.

[0245] 5) The washed membranes were detected by alkaline phosphataselabeling followed by NBT/BCIP colometric detection as described inmanufacture's detection procedure (Enzo Diagnostics, Inc.). Themembranes were blocked for one hour at room temperature with 1× blockingsolution, washed 3 times with 1× detection reagents for 10 min, washed 2times with 1× predevelopment reaction buffer for 5 min and thendeveloped the blots in developing solution for 30-45 min until the dotsappear. All reagents were provided by manufacture (Enzo Diagnostics,Inc). In Addition, large scale reverse Southern assay was also performedusing KPL southern hybridization and detection kit™ followingmanfacturer's instruction(KPL, Gaithersburg, Md.).

Example 7 Characterization of Clones—Northern Blot Analysis

[0246] Alternative to Southern Blot analysis, some membranes werehybridized and detected as described in the example of Northern blottingassays. Northern Hybridization was used to detect mRNA differentiallyexpressed in Nicotiana as follows.

[0247] A random priming method was used to prepare probes from clonedp450 (Megaprime™ DNA Labelling Systems, Amersham Biosciences).

[0248] The following components were mixed: 25 ng denatured DNAtemplate; 4 ul of each unlabeled dTTP, dGTP and dCTP; 5 ul of reactionbuffer; P³²-labelled DATP and 2 ul of Klenow I; and H₂O, to bring thereaction to 50 μl. The mixture was incubated in 37° C. for 1-4 hours,then stopped with 2 μl of 0.5 M EDTA. The probe was denatured byincubating at 95° C. for 5 minutes before use.

[0249] RNA samples were prepared from ethylene treated and non-treatedfresh leaves of several pairs of tobacco lines. In some cases poly A+enriched RNA was used. Approximately 15 μg total RNA or 1.8 μg mRNA(methods of RNA and mRNA extraction as described in Example 5) werebrought to equal volume with DEPC H₂O (5-10 μl). The same volume ofloading buffer (1× MOPS; 18.5% Formaldehyde; 50% Formamide; 4%Ficoll400; Bromophenolblue) and 0.5 μl EtBr (0.5 μg/μl) were added. Thesamples were subsequently denatured in preparation for separation of theRNA by electrophoresis.

[0250] Samples were subjected to electrophoresis on a formaldehyde gel(1% Agarose, 1×MOPS, 0.6 M Formaldehyde) with 1×MOP buffer (0.4 MMorpholinopropanesulfonic acid; 0.1 M Na-acetate-3× H2O; 10 mM EDTA;adjust to pH 7.2 with NaOH). RNA was transferred to a Hybond-N+ membrane(Nylon, Amersham Pharmacia Biotech) by capillary method in 10×SSC buffer(1.5 M NaCl; 0.15 M Na-citrate) for 24 hours. Membranes with RNA sampleswere UV-crosslinked (auto crosslink setting, 254 nm, Stratagene,Stratalinker) before hybridization.

[0251] The membrane was prehybridized for 1-4 hours at 42° C. with 5-10ml prehybridization buffer (5×SSC; 50% Formamide; 5×Denhardt's-solution; 1% SDS; 100 μg/ml heat-denatured sheared non-homologous DNA). Old prehybridization buffer was discarded, and newprehybridization buffer and probe were added. The hybridization wascarried out over night at 42° C. The membrane was washed for 15 minuteswith 2×SSC at room temperature, followed by a wash with 2×SSC.

[0252] A major focus of the invention was the discovery of novel genesthat may be induced as a result of ethylene treatment or play a key rolein tobacco leaf quality and constituents. As illustrated in the tablebelow, Northern blots and reverse Southern Blot were useful indetermining which genes were induced by ethylene treatment relative tonon-induced plants. Interestingly, not all fragments were affectedsimilarly in the converter and nonconverter. The cytochrome p450fragments of interest were partially sequenced to determine theirstructural relatedness. This information was used to subsequentlyisolate and characterize full length gene clones of interest. InducedmRNA Expression Ethylene Treatment Fragments Converter D56-AC7 (SEQ IDNo: 35) + D56-AG11 (SEQ ID No: 31) + D56-AC12 (SEQ ID No: 45) + D70A-AB5(SEQ ID No: 95) + D73-AC9 (SEQ ID No: 43) + D70A-AA12 (SEQ ID No: 131) +D73A-AG3 (SEQ ID No: 129) + D34-52 (SEQ ID No: 61) + D56-AG6 (SEQ ID No:51) +

[0253] Northern analysis was performed using full length clones ontobacco tissue obtained from converter and nonconverter burley linesthat were induced by ethylene treatment. The purpose was to identifythose full length clones that showed elevated expression in ethyleneinduced converter lines relative to ethylene induced converter linesrelative to ethylene induced nonconverter burley lines. By so doing, thefunctionality relationship of full length clones may be determined bycomparing biochemical differences in leaf constituents between converterand nonconverter lines. As shown in table below, six clones showedsignificantly higher expression, as denoted by ++ and +++, in converterethylene treated tissue than that of nonconverter treated tissue,denoted by +. All of these clones showed little or no expression inconverter and nonconverter lines that were not ethylene treated. FullLength Clones Converter Nonconverter D101-BA2 ++ + D207-AA5 ++ +D208-AC8 +++ + D237-AD1 ++ + D89-AB1 ++ + D90A-BB3 ++ +

Example 8 Immunodetection of P450S Encoded by the Cloned Genes

[0254] Peptide regions corresponding to 20-22 amino acids in length fromthree p450 clones were selected for 1) having lower or no homology toother clones and 2) having good hydrophilicity and antigenicity. Theamino acid sequences of the peptide regions selected from the respectivep450 clones are listed below. The synthesized peptides were conjugatedwith KHL and then injected into rabbits. Antisera were collected 2 and 4weeks after the 4^(th) injection (Alpha Diagnostic Intl. Inc. SanAntonio, Tex.). D234-AD1 DIDGSKSKLVKAHRKIDEILG D90a-BB3RDAFREKETFDENDVEELNY D89-AB1 FKNNGDEDRHFSQKLGDLADKY

[0255] Antisera were examined for crossreactivity to target proteinsfrom tobacco plant tissue by Western Blot analysis. Crude proteinextracts were obtained from ethylene treated (0 to 40 hours) middleleaves of converter and nonconverter lines. Protein concentrations ofthe extracts were determined using RC DC Protein Assay Kit (BIO-RAD)following the manufacturer's protocol.

[0256] Two micrograms of protein were loaded onto each lane and theproteins separated on 10%-20% gradient gels using the Laemmli SDS-PAGEsystem. The proteins were transferred from gels to PROTRAN®Nitrocellulose Transfer Membranes (Schleicher & Schuell) with theTrans-Blot® Semi-Dry cell (BIO-RAD). Target p450 proteins were detectedand visualized with the ECL Advances Western Blotting Detection Kit(Amersham Biosciences). Primary antibodies against the synthetic-KLHconjugates were made in rabbits. Secondary antibody against rabbit IgG,coupled with peroxidase, was purchased from Sigma. Both primary andsecondary antibodies were used at 1:1000 dilutions. Antibodies showedstrong reactivity to a single band on the Western Blots indicating thatthe antisera were monospecific to the target peptide of interest.Antisera were also crossreactive with synthetic peptides conjuated toKLH.

Example 9 Nucleic Acid Identity and Structure Relatedness of IsolatedNucleic Acid Fragments

[0257] Over 100 cloned p450 fragments were sequenced in conjunction withNorthern blot analysis to determine their structural relatedness. Theapproach used utilized forward primers based either of two common p450motifs located near the carboxyl-terminus of the p450 genes. The forwardprimers corresponded to cytochrome p450 motifs FXPERF or GRRXCP(A/G) asdenoted in FIG. 1. The reverse primers used standard primers from eitherthe plasmid, SP6 or T7 located on both arms of pGEM™ plasmid, or a polyA tail. The protocol used is described below.

[0258] Spectrophotometry was used to estimate the concentration ofstarting double stranded DNA following the manufacturer's protocol(Beckman Coulter). The template was diluted with water to theappropriate concentration, denatured by heating at 95° C. for 2 minutes,and subsequently placed on ice. The sequencing reaction was prepared onice using 0.5 to 10 μl of denatured DNA template, 2 μl of 1.6 pmole ofthe forward primer, 8 μl of DTCS Quick Start Master Mix and the totalvolume brought to 20 μl with water. The thermocycling program consistedof 30 cycles of the follow cycle: 96° C. for 20 seconds, 50° C. for 20seconds, and 60° C. for 4 minutes followed by holding at 4° C.

[0259] The sequence was stopped by adding 5 μl of stop buffer (equalvolume of 3M NaOAc and 100 mM EDTA and 1 μl of 20 mg/ml glycogen). Thesample was precipitated with 60 μl of cold 95% ethanol and centrifugedat 6000 g for 6 minutes. Ethanol was discarded. The pellet was 2 washeswith 200 μl of cold 70% ethanol. After the pellet was dry, 40 μl of SLSsolution was added and the pellet was resuspended. A layer of mineraloil was over laid. The sample was then, placed on the CEQ 8000 AutomatedSequencer for further analysis.

[0260] In order to verify nucleic acid sequences, nucleic acid sequencewas re-sequenced in both directions using forward primers to the FXPERFor GRRXCP(A/G) region of the p450 gene or reverse primers to either theplasmid or poly A tail. All sequencing was performed at least twice inboth directions.

[0261] The nucleic acid sequences of cytochrome p450 fragments werecompared to each other from the coding region corresponding to the firstnucleic acid after the region encoding the GRRXCP(A/G) motif through tothe stop codon.

[0262] This region was selected as an indicator of genetic diversityamong p450 proteins. A large number of genetically distinct p450 genes,in excess of 70 genes, were observed, similar to that of other plantspecies. Upon comparison of nucleic acid sequences, it was found thatthe genes could be placed into distinct sequences groups based on theirsequence identity. It was found that the best unique grouping of p450members was determined to be those sequences with 75% nucleic acididentity or greater (shown in Table I). Reducing the percentage identityresulted in significantly larger groups. A preferred grouping wasobserved for those sequences with 81% nucleic acid identity or greater,a more preferred grouping 91% nucleic acid identity or greater, and amost preferred grouping for those sequences 99% nucleic acid identity ofgreater. Most of the groups contained at least two members andfrequently three or more members. Others were not repeatedly discoveredsuggesting that approach taken was able to isolated both low and highexpressing mRNA in the tissue used.

[0263] Based on 75% nucleic acid identity or greater, two cytochromep450 groups were found to contain nucleic acid sequence identity topreviously tobacco cytochrome genes that genetically distinct from thatwithin the group. Group 23, showed nucleic acid identity, within theparameters used for Table I, to prior GenBank sequences of GI:1171579(CAA64635) and GI:14423327 (or AAK62346) by Czernic et al and Ralston etal, respectively. GI:1171579 had nucleic acid identity to Group 23members ranging 96.9% to 99.5% identity to members of Group 23 whileGI:14423327 ranged 95.4% to 96.9% identity to this group. The members ofGroup 31 had nucleic acid identity ranging from 76.7% to 97.8% identityto the GenBank reported sequence of GI:14423319 (AAK62342) by Ralston etal. None of the other p450 identity groups of Table 1 containedparameter identity, as used in Table 1, to Nicotiana p450s genesreported by Ralston et al, Czernic et al., Wang et al or LaRosa andSmigocki.

[0264] As shown in FIG. 76, consensus sequence with appropriate nucleicacid degenerate probes could be derived for group to preferentiallyidentify and isolate additional members of each group from Nicotianaplants. TABLE I Nicotiana p450 Nucleic Acid Sequence Identity GroupsGROUP FRAGMENTS  1 D58-BG7 (SEQ ID No.:1), D58-AB1 (SEQ ID No.:3);D58-BE4 (SEQ ID No.:7)  2 D56-AH7 (SEQ ID No.:9); D13a-5 (SEQ ID No.:11) 3 D56-AG10 (SEQ ID No.:13); D35-33 (SEQ ID No.:15); D34-62 (SEQ IDNo.:17)  4 D56-AA7 (SEQ ID No.:19); D56-AE1 (SEQ ID No.:21); 185-BD3(SEQ ID No.:143)  5 D35-BB7 (SEQ ID No.:23); D177-BA7 (SEQ ID No.:25);D56A-AB6 (SEQ ID No.:27); D144-AE2 (SEQ ID No.:29)  6 D56-AG11 (SEQ IDNo.:31); D179-AA1 (SEQ ID No.:33)  7 D56-AC7 (SEQ ID No.:35); D144-AD1(SEQ ID No.:37)  8 D144-AB5 (SEQ ID No.:39)  9 D181-AB5 (SEQ ID No.:41);D73-Ac9 (SEQ ID No.:43) 10 D56-AC12 (SEQ ID No.:45) 11 D58-AB9 (SEQ IDNo.:47); D56-AG9 (SEQ ID No.:49); D56-AG6 (SEQ ID No.:51); D35-BG11 (SEQID No.:53); D35-42 (SEQ ID No.:55); D35-BA3 (SEQ ID No.:57); D34-57 (SEQID No.:59); D34-52 (SEQ ID No.:61); D34-25 (SEQ ID No.:63) 12 D56-AD10(SEQ ID No.:65) 13 56-AA11 (SEQ ID No.:67) 14 D177-BD5 (SEQ ID No.:69);D177-BD7 (SEQ ID No.:83) 15 D56A-AG10 (SEQ ID No.:71); D58-BC5 (SEQ IDNo.:73); D58-AD12 (SEQ ID No.:75) 16 D56-AC11 (SEQ ID No.:77); D35-39(SEQ ID No.:79); D58-BH4 (SEQ ID No.:81); D56-AD6 (SEQ ID No.:87) 17D73A-AD6 (SEQ ID No.:89); D70A-BA11 (SEQ ID No.:91) 18 D70A-AB5 (SEQ IDNo.:95); D70A-AA8 (SEQ ID No.:97) 19 D70A-AB8 (SEQ ID No.:99); D70A-BH2(SEQ ID No.:101); D70A-AA4 (SEQ ID No.:103) 20 D70A-BA1 (SEQ IDNo.:105); D70A-BA9 (SEQ ID No.:107) 21 D70A-BD4 (SEQ ID No.:109) 22D181-AC5 (SEQ ID No.:111); D144-AH1 (SEQ ID No.:113); D34-65 (SEQ IDNo.:115) 23 D35-BG2 (SEQ ID No.:117) 24 D73A-AH7 (SEQ ID No.:119) 25D58-AA1 (SEQ ID No.:121); D185-BC1 (SEQ ID No.:133); D185-BG2 (SEQ IDNo.:135) 26 D73-AE10 (SEQ ID No.:123) 27 D56-AC12 (SEQ ID No.:125) 28D177-BF7 (SEQ ID No.:127); D185-BE1 (SEQ ID No.:137); D185-BD2 (SEQ IDNo.:139) 29 D73A-AG3 (SEQ ID No.:129) 30 D70A-AA12 (SEQ ID No.:131);D176-BF2 (SEQ ID No.:85) 31 D176-BC3 (SEQ ID No.:145) 32 D176-BB3 (SEQID No.:147)

[0265] D186-AH4 (SEQ ID No.:5)

Example 10 Related Amino Acid Sequence Identity of Isolated Nucleic AcidFragments

[0266] The amino acid sequences of nucleic acid sequences obtained forcytochrome p450 fragments from Example 8 were deduced. The deducedregion corresponded to the amino acid immediately after the GXRXCP(A/G)sequence motif to the end of the carboxyl-terminus, or stop codon. Uponcomparison of sequence identity of the fragments, a unique grouping wasobserved for those sequences with 70% amino acid identity or greater. Apreferred grouping was observed for those sequences is with 80% aminoacid identity or greater, more preferred with 90% amino acid identity orgreater, and a most preferred grouping for those sequences 99% aminoacid identity of greater. The groups and corresponding amino acidsequences of group members are shown in FIG. 2. Several of the uniquenucleic acid sequences were found to have complete amino acid identityto other fragments and therefore only one member with the identicalamino acid was reported.

[0267] The amino acid identity for Group 19 of Table II corresponded tothree distinct groups based on their nucleic acid sequences. The aminoacid sequences of each group member and their identity is shown in FIG.77. The amino acid differences are appropriated marked.

[0268] At least one member of each amino acid identity group wasselected for gene cloning and functional studies using plants. Inaddition, group members that are differentially affected by ethylenetreatment or other biological differences as assessed by Northern andSouthern analysis were selected for gene cloning and functional studies.To assist in gene cloning, expression studies and whole plantevaluations, peptide specific antibodies will be prepared on sequenceidentity and differential sequence. TABLE II Nicotiana p450 Amino AcidSequence Identity Groups GROUP FRAGMENTS  1 D58-BG7 (SEQ ID No.:2),D58-AB1 (SEQ ID No.:4)  2 D58-BE4 (SEQ ID No.:8)  3 D56-AH7 (SEQ IDNo.:10); D13a-5 (SEQ ID No.:12)  4 D56-AG10 (SEQ ID No.:14); D34-62 (SEQID No.:18)  5 D56-AA7 (SEQ ID No.:20); D56-AE1 (SEQ ID No.:22); 185- BD3(SEQ ID No.:144)  6 D35-BB7 (SEQ ID No.:24); D177-BA7 (SEQ ID No.:26);D56A-AB6 (SEQ ID No.:28); D144-AE2 (SEQ ID No.:30)  7 D56-AG11 (SEQ IDNo.:32); D179-AA1 (SEQ ID No.:34)  8 D56-AC7 (SEQ ID No.:36); D144-AD1(SEQ ID No.:38)  9 D144-AB5 (SEQ ID No.:40) 10 D181-AB5 (SEQ ID No.:42);D73-Ac9 (SEQ ID No.:44) 11 D56-AC12 (SEQ ID No.:46) 12 D58-AB9 (SEQ IDNo.:48); D56-AG9 (SEQ ID No.:50); D56- AG6 (SEQ ID No.:52); D35-BG11(SEQ ID No.:54); D35-42 (SEQ ID No.:56); D35-BA3 (SEQ ID No.:58); D34-57(SEQ ID No.:60); D34-52 (SEQ ID No.:62) 13 D56AD10 (SEQ ID No.:66) 1456-AA11 (SEQ ID No.:68) 15 D177-BD5 (SEQ ID No.:70); D177-BD7 (SEQ IDNo.:84) 16 D56A-AG10 (SEQ ID No.:72); D58-BC5 (SEQ ID No.:74); D58-AD12(SEQ ID No.:76) 17 D56-AC11 (SEQ ID No.:78); D56-AD6 (SEQ ID No.:88) 18D73A-AD6 (SEQ ID No.90:) 19 D70A-AB5 (SEQ ID No.:96); D70A-AB8 (SEQ IDNo.:100); D70A-BH2 (SEQ ID No.:102); D70A-AA4 (SEQ ID No.:104); D70A-BA1 (SEQ ID No.:106); D70A-BA9 (SEQ ID No.:108) 20 D70A-BD4 (SEQ IDNo.:110) 21 D181-AC5 (SEQ ID No.:112); D144-AH1 (SEQ ID No.:114); D34-65(SEQ ID No.:116) 22 D35-BG2 (SEQ ID No.:118) 23 D73A-AH7 (SEQ IDNo.:120) 24 D58-AA1 (SEQ ID No.:122); D185-BC1 (SEQ ID No.:134);D185-BG2 (SEQ ID No.:136) 25 D73-AE10 (SEQ ID No.:124) 26 D56-AC12 (SEQID No.:126) 27 D177-BF7 (SEQ ID No.:128); 185-BD2 (SEQ ID No.:140) 28D73A-AG3 (SEQ ID No.:130) 29 D70A-AA12 (SEQ ID No.:132); D176-BF2 (SEQID No.:86) 30 D176-BC3 (SEQ ID No.:146) 31 D176-BB3 (SEQ ID No.:148) 32D186-AH4 (SEQ ID No.:6)

Example 11 Related Amino Acid Sequence Identity of Full Length Clones

[0269] The nucleic acid sequence of full length Nicotiana genes clonedin Example 5 were deduced for their entire amino acid sequence.Cytochrome p450 genes were identified by the presence of three conservedp450 domain motifs, which corresponded to UXXRXXZ, PXRFXF or GXRXC atthe carboxyl-terminus where U is E or K, X is any amino acid and Z is P,T, S or M. It was also noted that two of the clones appeared nearlycomplete but lacked the appropriate stop codon, D130-AA1 and D101-BA2,however but both contained all three p450 cytochrome domains. All p450genes were characterized for amino acid identity using a BLAST programcomparing their full length sequences to each other and to known tobaccogenes. The program used the NCBI special BLAST tool (Align two sequences(b12seq), http://www.ncbi.nlm.nih.gov/blast/b12seq/b12.html). Twosequences were aligned under BLASTN without filter for nucleic acidsequences and BLASTP for amino acid sequences. Based on their percentageamino acid identity, each sequence was grouped into identity groupswhere the grouping contained members that shared at least 85% identitywith another member. A preferred grouping was observed for thosesequences with 90% amino acid identity or greater, a more preferredgrouping had 95% amino acid identity or greater, and a most preferredgrouping had those sequences 99% amino acid identity or greater. Usingthese criteria, 25 unique groups were identified and are depicted inTable III.

[0270] Within the parameters used for Table III for amino acid identity,three groups were found to contain greater than 85% or greater identityto known tobacco genes. Members of Group 5 had up to 96% amino acididentity for full length sequences to prior GenBank sequences ofGI:14423327 (or AAK62346) by Ralston et al. Group 23 had up to 93% aminoacid identity to GI:14423328 (or AAK62347) by Ralston et al. and Group24 had 92% identity to GI:14423318 (or AAK62343) by Ralston et al. TABLEIII Amino Acid Sequence Identity Groups of Full Length Nicotiana p450Genes  1 D208-AD9 (SEQ. ID. No. 224); D120-AH4 (SEQ. ID. No. 180);D121-AA8 (SEQ. ID. No. 182), D122-AF10 (SEQ. ID. No. 184); D103-AH3(SEQ. ID. No. 222); D208-AC8 (SEQ. ID. No. 218); D-235-ABI (SEQ. ID. No.246)  2 D244-AD4 (SEQ. ID. No. 250); D244-AB6 (SEQ. ID. No. 274) ;D285-AA8; D285- AB9; D268-AE2 (SEQ. ID. No. 270)  3 D100A-AC3 (SEQ. ID.No. 168); D100A-BE2  4 D205-BE9 (SEQ. ID. No. 276); D205-BG9 (SEQ. ID.No. 202); D205-AH4 (SEQ. ID. No. 294)  5 D259-AB9 (SEQ. ID. No. 260) ;D257-AE4 (SEQ. ID. No. 268) ; D147-AD3 (SEQ. ID. No. 194)  6 D249-AE8(SEQ. ID. No. 256); D-248-AA6 (SEQ. ID. No. 254)  7 D233-AG7 (SEQ. ID.No. 266; D224-BD11 (SEQ. ID. No. 240); DAF10  8 D105-AD6 (SEQ. ID. No.172); D215-AB5 (SEQ. ID. No. 220); D135-AE1 (SEQ. ID. No. 190)  9D87A-AF3 (SEQ. ID. No. 216), D210-BD4 (SEQ. ID. No. 262) 10 D89-AB1(SEQ. ID. No. 150); D89-AD2 (SEQ. ID. No. 152); 163-AG11 (SEQ. ID. No.198); 163-AF12 (SEQ. ID. No. 196) 11 D267-AF10 (SEQ. ID. No. 296);D96-AC2 (SEQ. ID. No. 160); D96-AB6 (SEQ. ID. No. 158); D207-AA5 (SEQ.ID. No. 204); D207-AB4 (SEQ. ID. No. 206); D207-AC4 (SEQ. ID. No. 208)12 D98-AG1 (SEQ. ID. No. 164); D98-AA1 (SEQ. ID. No. 162) 13 D209-AA12(SEQ. ID. No. 212); D209- AA11; D209-AH10 (SEQ. ID. No. 214); D209-AH12(SEQ. ID. No. 232); D90a-BB3 (SEQ. ID. No. 154) 14 D129-AD10 (SEQ. ID.No. 188); D104A-AE8 (SEQ. ID. No. 170) 15 D228-AH8 (SEQ. ID. No. 244);D228-AD7 (SEQ. ID. No. 241), D250- AC11 (SEQ. ID. No. 258); D247-AH1(SEQ. ID. No. 252) 16 D128-AB7 (SEQ. ID. No. 186) ; D243-AA2 (SEQ. ID.No. 248); D125-AF11 (SEQ. ID. No. 228) 17 D284-AH5 (SEQ. ID. No. 298);D110-AF12 (SEQ. ID. No. 176) 18 D221-BB8 (SEQ. ID. No. 234) 19 D222-BH4(SEQ. ID. No. 236) 20 D134-AE11 (SEQ. ID. No. 230) 21 D109-AH8 (SEQ. ID.No. 174) 22 D136-AF4 (SEQ. ID. No. 278) 23 D237-AD1 (SEQ. ID. No. 226)24 D112-AA5 (SEQ. ID. No. 178) 25 D283-AC1 (SEQ. ID. No. 272)

[0271] The full length genes were further grouped based on the highlyconversed amino acid homology between UXXRXXZ p450 domain and GXRXC p450domain near the end the carboxyl-terminus. As shown in FIG. 3,individual clones were aligned for their sequence homology between theconserved domains relative to each other and placed in distinct identitygroups. In several cases, although the nucleic acid sequence of theclone was unique, the amino acid sequence for the region was identical.The preferred grouping was observed for those sequences with 90% aminoacid identity or greater, a more preferred group had 95% amino acididentity or greater, and a most preferred grouping had those sequences99% amino acid identity of greater. The final grouping was similar tothat based on the percent identity for the entire amino acid sequence ofthe clones except for Group 17 (of Table III) which was divided into twodistinct groups.

[0272] Within the parameters used for amino acid identity in Table IV,three groups were found to contain 90% or greater identity to knowntobacco genes. Members of Group 5 had up to 93.4% amino acid identityfor full length sequences to prior GenBank sequences of GI:14423326(AAK62346) by Ralston et al. Group 23 had up to 91.8% amino acididentity to GI:14423328 (or AAK62347) by Ralston et al. and Group 24 had98.8% identity to GI:14423318 (or AAK62342) by Ralston et al. TABLE IVAmino Acid Sequence Identity Groups of Regions between Conserved Domainsof Nicotiana p450 Genes  1 1 D208-AD9 (SEQ. ID. No. 224); D120-AH4 (SEQ.ID. No. 180); D121-AA8 (SEQ. ID. No. 182), D122-AF10 (SEQ. ID. No. 184);D103-AH3 (SEQ. ID. No. 222); D208-AC8 (SEQ. ID. No. 218); D-235-ABI(SEQ. ID. No. 246)  2 D244-AD4 (SEQ. ID. No. 250); D244-AB6 (SEQ. ID.No. 274); D285-AA8; D285-AB9; D268-AE2 (SEQ. ID. No. 270)  3 D100A-AC3(SEQ. ID. No. 168); D100A-BE2  4 D205-BE9 (SEQ. ID. No. 276); D205-BG9(SEQ. ID. No. 202); D205-AH4 (SEQ. ID. No. 294)  5 D259-AB9 (SEQ. ID.No. 260) ; D257-AE4 (SEQ. ID. No. 268); D147-AD3 (SEQ. ID. No. 194)  6D249-AE8 (SEQ. ID. No. 256); D-248-AA6 (SEQ. ID. No. 254)  7 D233-AG7(SEQ. ID. No. 266; D224-BD11 (SEQ. ID. No. 240) ; DAF10  8 D105-AD6(SEQ. ID. No. 172); D215-AB5 (SEQ. ID. No. 220) ; D135-AE1 (SEQ. ID. No.190)  9 D87A-AF3 (SEQ. ID. No. 216), D210-BD4 (SEQ. ID. No. 262) 10D89-AB1 (SEQ. ID. No. 150); D89-AD2 (SEQ. ID. No. 152); 163-AG11 (SEQ.ID. No. 198); 163-AF12 (SEQ. ID. No. 196) 11 D267-AF10 (SEQ. ID. No.296); D96-AC2 (SEQ. ID. No. 160); D96-AB6 (SEQ. ID. No. 158); D207-AA5(SEQ. ID. No. 204); D207-AB4 (SEQ. ID. No. 206); D207-AC4 (SEQ. ID. No.208) 12 D98-AG1 (SEQ. ID. No. 164); D98-AA1 (SEQ. ID. No. 162) 13D209-AA12 (SEQ. ID. No. 212); D209-AA11; D209-AH10 (SEQ. ID. No. 214);D209-AH12 (SEQ. ID. No. 232); D90a-BB3 (SEQ. ID. No. 154) 14 D129-AD10(SEQ. ID. No. 188); D104A-AE8 (SEQ. ID. No. 170) 15 D228-AH8 (SEQ. ID.No. 244); D228-AD7 (SEQ. ID. No. 241), D250-AC11 (SEQ. ID. No. 258);D247-AH1 (SEQ. ID. No. 252) 16 D128-AB7 (SEQ. ID. No. 186) ; D243-AA2(SEQ. ID. No. 248); D125-AF11 (SEQ. ID. No. 228) 17 D284-AH5 (SEQ. ID.No. 298); D110-AF12 (SEQ. ID. No. 176) 18 D221-BB8 (SEQ. ID. No. 234) 19D222-BH4 (SEQ. ID. No. 236) 20 D134-AE11 (SEQ. ID. No. 230) 21 D109-AH8(SEQ. ID. No. 174) 22 D136-AF4 (SEQ. ID. No. 278) 23 D237-AD1 (SEQ. ID.No. 226) 24 D112-AA5 (SEQ. ID. No. 178) 25 D283-AC1 (SEQ. ID. No. 272)26 D110-AF12 (SEQ. ID. No. 176)

Example 12 Nicotiana Cytochrome P450 Clones Lacking One or more of theTobacco Cytochrome P450 Specific Domains

[0273] Four clones had high nucleic acid homology, ranging 90% to 99%nucleic acid homology, to other tobacco cytochrome genes reported inTable III. The four clones included D136-AD5, D138-AD12, D243-AB3 andD250-AC11. However, due to a nucleotide frameshift these genes did notcontain one or more of three C-terminus cytochrome p450 domains and wereexcluded from identity groups presented in Table III or Table IV.

[0274] The amino acid identity of one clone, D95-AG1, did not containthe third domain, GXRXC, used to group p450 tobacco genes in Table IIIor Table IV. The nucleic acid homology of this clone had low homology toother tobacco cytochrome genes. This clone represents a novel anddifferent group of cytochrome p450 genes in Nicotiana.

Example 13 Use of Nicotiana Cytochrome P450 Fragments and Clones inAltered Regulartion of Tobacco Properties

[0275] The use of tobacco p450 nucleic acid fragments or whole genes areuseful in identifying and selecting those plants that have alteredtobacco phenotypes or tobacco constituents and, more importantly,altered metabolites. Transgenic tobacco plants are generated by avariety of transformation systems that incorporate nucleic acidfragments or full length genes, selected from those reported herein, inorientations for either down-regulation, for example anti-senseorientation, or over-expression for example, sense orienation. Forover-expression to full length genes, any nucleic acid sequence thatencodes the entire or a functional part or amino acide sequence of thefull-length genes described in this invention are desired that areeffective for increasing the expression of a certain enzyme and thusresulting in phenotypic effect within Nicotiana. Nicotiana lines thatare homozygous lines are obtained through a series of backcrossing andassessed for phenotypic changes including, but not limited to, analysisof endogenous p450 RNA, transcripts, p450 expressed peptides andconcentrations of plant metabolites using techniques commonly avaiableto one having ordinary skill in the art.

[0276] The changes exhibited in the tobacco plans provide information onthe functional role of the selected gene of interest or are of a utilityas a preffered Nicotiana plant species.

[0277] Numerous modifications and variations in practice of theinvention are expected to occur to those skilled in the art uponconsideration of the foregoing detailed description of the invention.Consequently, such modifications and variations are intended to beincluded within the scope of the following claims.

1 298 1 207 DNA NICOTIANATABACUM 1 gcacaacttg ctatcaactt ggtcacatctatgttgggtc atttgttgca tcattttaca 60 tgggctccgg ccccgggggt taacccggaggatattgact tggaggagag ccctggaaca 120 gtaacttaca tgaaaaatcc aatacaagctattccaactc caagattgcc tgcacacttg 180 tatggacgtg tgccagtgga tatgtaa 207 268 PRT NICOTIANATABACUM 2 Ala Gln Leu Ala Ile Asn Leu Val Thr Ser MetLeu Gly His Leu Leu 1 5 10 15 His His Phe Thr Trp Ala Pro Ala Pro GlyVal Asn Pro Glu Asp Ile 20 25 30 Asp Leu Glu Glu Ser Pro Gly Thr Val ThrTyr Met Lys Asn Pro Ile 35 40 45 Gln Ala Ile Pro Thr Pro Arg Leu Pro AlaHis Leu Tyr Gly Arg Val 50 55 60 Pro Val Asp Met 65 3 207 DNANICOTIANATABACUM 3 gcacaacttg ctatcaactt ggtcacatct atgttgggtcatttgttgca tcattttacg 60 tgggctccgc ccccgggggt taacccggag aatattgacttggaggagag ccctggaaca 120 gtaacttaca tgaaaaatcc aatacaagct attcctactccaagattgcc tgcacacttg 180 tatggacgtg tgccagtgga tatgtaa 207 4 68 PRTNICOTIANATABACUM 4 Ala Gln Leu Ala Ile Asn Leu Val Thr Ser Met Leu GlyHis Leu Leu 1 5 10 15 His His Phe Thr Trp Ala Pro Pro Pro Gly Val AsnPro Glu Asn Ile 20 25 30 Asp Leu Glu Glu Ser Pro Gly Thr Val Thr Tyr MetLys Asn Pro Ile 35 40 45 Gln Ala Ile Pro Thr Pro Arg Leu Pro Ala His LeuTyr Gly Arg Val 50 55 60 Pro Val Asp Met 65 5 183 DNA NICOTIANATABACUM 5atgaattatt cattgcaagt ggaacacctt tcaattgctc atatgatcca aggtttcagt 60tttgcaacta cgaccaatga gcctttggat atgaaacaag gtgtgggttt aactttacca 120aagaagactg atgttgaagt gctaattaca cctcgccttc ctcctacgct ttatcaatat 180taa 183 6 60 PRT NICOTIANATABACUM 6 Met Asn Tyr Ser Leu Gln Val Glu HisLeu Ser Ile Ala His Met Ile 1 5 10 15 Gln Gly Phe Ser Phe Ala Thr ThrThr Asn Glu Pro Leu Asp Met Lys 20 25 30 Gln Gly Val Gly Leu Thr Leu ProLys Lys Thr Asp Val Glu Val Leu 35 40 45 Ile Thr Pro Arg Leu Pro Pro ThrLeu Tyr Gln Tyr 50 55 60 7 132 DNA NICOTIANATABACUM 7 gcacaacttgctatcaactt ggtcacatct atgttgggtc atttgttcat cattttacat 60 gggctccggccccgggggtt aacccggagg atattgactt ggaggagagc cctggaacag 120 taacttacat ga132 8 40 PRT NICOTIANATABACUM 8 Ala Gln Leu Ala Ile Asn Leu Val Thr SerMet Leu Gly His Leu Phe 1 5 10 15 Ile Ile Leu His Gly Leu Arg Pro ArgGly Leu Thr Arg Arg Ile Leu 20 25 30 Thr Trp Arg Arg Ala Leu Glu Gln 3540 9 189 DNA NICOTIANATABACUM 9 gaaggattgg ctgttcgaat ggttgccttgtcattgggat gtattattca atgttttgat 60 tggcaacgaa tcggcgaaga attggttgatatgactgaag gaactggact tactttgcct 120 aaagctcaac ctttggtggc caagtgtagcccacgaccta aaatggctaa tcttctctct 180 cagatttga 189 10 62 PRTNICOTIANATABACUM 10 Glu Gly Leu Ala Val Arg Met Val Ala Leu Ser Leu GlyCys Ile Ile 1 5 10 15 Gln Cys Phe Asp Trp Gln Arg Ile Gly Glu Glu LeuVal Asp Met Thr 20 25 30 Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln ProLeu Val Ala Lys 35 40 45 Cys Ser Pro Arg Pro Lys Met Ala Asn Leu Leu SerGln Ile 50 55 60 11 189 DNA NICOTIANATABACUM 11 gaaggattgg ctattcgaatggttgcattg tcattgggat gtattattca atgctttgat 60 tggcaacgac ttggggaaggattggttgat aagactgaag gaactggact tactttgcct 120 aaagctcaac ctttagtggccaagtgtagc ccacgaccta taatggctaa tcttctttct 180 cagatttga 189 12 62 PRTNICOTIANATABACUM 12 Glu Gly Leu Ala Ile Arg Met Val Ala Leu Ser Leu GlyCys Ile Ile 1 5 10 15 Gln Cys Phe Asp Trp Gln Arg Leu Gly Glu Gly LeuVal Asp Lys Thr 20 25 30 Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln ProLeu Val Ala Lys 35 40 45 Cys Ser Pro Arg Pro Ile Met Ala Asn Leu Leu SerGln Ile 50 55 60 13 183 DNA NICOTIANATABACUM 13 ataggttttg cgactttagtgacacatctg acttttggtc gcttgcttca aggttttgat 60 tttagtaagc catcaaacacgccaattgac atgacagaag gcgtaggcgt tactttgcct 120 aaggttaatc aagttgaagttctaattacc cctcgtttac cttctaagct ttatttattt 180 tga 183 14 60 PRTNICOTIANATABACUM 14 Ile Gly Phe Ala Thr Leu Val Thr His Leu Thr Phe GlyArg Leu Leu 1 5 10 15 Gln Gly Phe Asp Phe Ser Lys Pro Ser Asn Thr ProIle Asp Met Thr 20 25 30 Glu Gly Val Gly Val Thr Leu Pro Lys Val Asn GlnVal Glu Val Leu 35 40 45 Ile Thr Pro Arg Leu Pro Ser Lys Leu Tyr Leu Phe50 55 60 15 178 DNA NICOTIANATABACUM 15 ataggctttg cgactttagt gacacatctgacttttggtc gcttgcttca aggttttgat 60 tttagtaagc catcaaacac gccaattgacatgacagaag gcgtaggcgt tactttgcct 120 aaggttaatc aagttgaagt tctaattacccctcgtttac cttctaagct ttatttat 178 16 59 PRT NICOTIANATABACUM 16 Ile GlyPhe Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu 1 5 10 15 GlnGly Phe Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr 20 25 30 GluGly Val Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu 35 40 45 IleThr Pro Arg Leu Pro Ser Lys Leu Tyr Leu 50 55 17 183 DNANICOTIANATABACUM 17 ataaattttg cgactttagt gacacatctg acttttggtcgcttgcttca aggttttgat 60 tttagtacgc catcaaacac gccaatagac atgacagaaggcgtaggcgt tactttgcct 120 aaggtaaatc aagtggaagt tctaattagc cctcgtttaccttctaagct ttatgtattc 180 tga 183 18 60 PRT NICOTIANATABACUM 18 Ile AsnPhe Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu 1 5 10 15 GlnGly Phe Asp Phe Ser Thr Pro Ser Asn Thr Pro Ile Asp Met Thr 20 25 30 GluGly Val Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu 35 40 45 IleSer Pro Arg Leu Pro Ser Lys Leu Tyr Val Phe 50 55 60 19 171 DNANICOTIANATABACUM 19 attatacttg cattgccaat tcttggcatc actttgggacgtttggttca gaactttgag 60 ctgttgcctc ctccaggcca gtcgaagctc gacaccacagagaaaggtgg acagttcagt 120 ctccacattt tgaagcattc caccattgtg ttgaaaccaaggtctttctg a 171 20 56 PRT NICOTIANATABACUM 20 Ile Ile Leu Ala Leu ProIle Leu Gly Ile Thr Leu Gly Arg Leu Val 1 5 10 15 Gln Asn Phe Glu LeuLeu Pro Pro Pro Gly Gln Ser Lys Leu Asp Thr 20 25 30 Thr Glu Lys Gly GlyGln Phe Ser Leu His Ile Leu Lys His Ser Thr 35 40 45 Ile Val Leu Lys ProArg Ser Phe 50 55 21 171 DNA NICOTIANATABACUM 21 attatacttg cattgccaattcttggcatt actttgggac gtttggttca gaactttgag 60 ctgttgcctc ctccaggccagtcgaagctc gacaccacag agaaaggtgg acagttcagt 120 ctccatattt tgaagcattccaccattgtg ttgaaaccaa ggtcttgctg a 171 22 56 PRT NICOTIANATABACUM 22 IleIle Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu Val 1 5 10 15Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp Thr 20 25 30Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser Thr 35 40 45Ile Val Leu Lys Pro Arg Ser Cys 50 55 23 172 DNA NICOTIANATABACUM 23tattgcactt ggggttgcat caatggaact tgcattgtca aatcttcttt atgcatttga 60ttgggagtta ccttttggaa tgaaaaaaga agacattgac acaaacgcca ggcctggaat 120taccatgcat aagaaaaacg aactttatct tatccctaaa aattatctat ag 172 24 63 PRTNICOTIANATABACUM 24 Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu SerAsn Leu Leu 1 5 10 15 Tyr Ala Phe Asp Trp Glu Leu Pro Phe Gly Met LysLys Glu Asp Ile 20 25 30 Asp Thr Asn Ala Arg Pro Gly Ile Thr Met His LysLys Asn Glu Leu 35 40 45 Tyr Leu Ile Pro Lys Asn Tyr Leu Pro Ser Lys LeuTyr Leu Phe 50 55 60 25 171 DNA NICOTIANATABACUM 25 attgcacttggggttgcatc catggaactt gctttgtcaa atcttcttta tgcatttgat 60 tgggagttaccttacggagt gaaaaaagaa aacattgaca caaatgtcag gcctggaatt 120 accatgcataagaaaaacga actttgcctt atccctagaa attatctata g 171 26 56 PRTNICOTIANATABACUM 26 Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu SerAsn Leu Leu 1 5 10 15 Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val LysLys Glu Asn Ile 20 25 30 Asp Thr Asn Val Arg Pro Gly Ile Thr Met His LysLys Asn Glu Leu 35 40 45 Cys Leu Ile Pro Arg Asn Tyr Leu 50 55 27 174DNA NICOTIANATABACUM 27 ggtattgcac ttggggttgc atccatggaa cttgctttgtcaaatcttct ttatgcattt 60 gattgggagt tgccttatgg agtgaaaaaa gaagacatcgacacaaacgt taggcctgga 120 attgccatgc acaagaaaaa cgaactttgc cttgtcccaaaaaattattt ataa 174 28 56 PRT NICOTIANATABACUM 28 Ile Ala Leu Gly ValAla Ser Met Glu Leu Ala Leu Ser Asn Leu Leu 1 5 10 15 Tyr Ala Phe AspTrp Glu Leu Pro Tyr Gly Val Lys Lys Glu Asp Ile 20 25 30 Asp Thr Asn ValArg Pro Gly Ile Ala Met His Lys Lys Asn Glu Leu 35 40 45 Cys Leu Val ProLys Asn Tyr Leu 50 55 29 201 DNA NICOTIANATABACUM 29 attgcacttggggttgcatc catggaactt gctttgtcaa atcttcttta tgcatttgat 60 tgggagttgccttatggagt gaaaaaagaa gacatcgaca caaacgttag gcctggaatt 120 gccatgcacaagaaaaacga actttgcctt gtcccaaaaa aattatttat aaattatatt 180 gggacgtggatctcatgcta g 201 30 66 PRT NICOTIANATABACUM 30 Ile Ala Leu Gly Val AlaSer Met Glu Leu Ala Leu Ser Asn Leu Leu 1 5 10 15 Tyr Ala Phe Asp TrpGlu Leu Pro Tyr Gly Val Lys Lys Glu Asp Ile 20 25 30 Asp Thr Asn Val ArgPro Gly Ile Ala Met His Lys Lys Asn Glu Leu 35 40 45 Cys Leu Val Pro LysLys Leu Phe Ile Asn Tyr Ile Gly Thr Trp Ile 50 55 60 Ser Cys 65 31 183DNA NICOTIANATABACUM 31 atttcgtttg gtttagctaa tgcttatttg ccattggctcaattacttta tcactttgat 60 tgggaactcc ccactggaat caaaccaagc gacttggacttgactgagtt ggttggagta 120 actgccgcta gaaaaagtga cctttacttg gttgcgactccttatcaacc tcctcaaaac 180 tga 183 32 60 PRT NICOTIANATABACUM 32 Ile SerPhe Gly Leu Ala Asn Ala Tyr Leu Pro Leu Ala Gln Leu Leu 1 5 10 15 TyrHis Phe Asp Trp Glu Leu Pro Thr Gly Ile Lys Pro Ser Asp Leu 20 25 30 AspLeu Thr Glu Leu Val Gly Val Thr Ala Ala Arg Lys Ser Asp Leu 35 40 45 TyrLeu Val Ala Thr Pro Tyr Gln Pro Pro Gln Asn 50 55 60 33 183 DNANICOTIANATABACUM 33 atttcgtttg gcttagctaa tgcttatttg ccattggctcaattactata tcacttcgat 60 tggaaactcc ctgctggaat cgaaccaagc gacttggacttgactgagtt ggttggagta 120 actgccgcta gaaaaagtga cctttacttg gttgcgactccttatcaacc tcctcaaaag 180 tga 183 34 60 PRT NICOTIANATABACUM 34 Ile SerPhe Gly Leu Ala Asn Ala Tyr Leu Pro Leu Ala Gln Leu Leu 1 5 10 15 TyrHis Phe Asp Trp Lys Leu Pro Ala Gly Ile Glu Pro Ser Asp Leu 20 25 30 AspLeu Thr Glu Leu Val Gly Val Thr Ala Ala Arg Lys Ser Asp Leu 35 40 45 TyrLeu Val Ala Thr Pro Tyr Gln Pro Pro Gln Lys 50 55 60 35 220 DNANICOTIANATABACUM 35 atgctatttg gtttagctaa tgttggacaa cctttagctcagttacttta tcacttcgat 60 tggaaactcc ctaatggaca aagtcatgag aatttcgacatgactgagtc acctggaatt 120 tctgctacaa gaaaggatga tcttgttttg attgccactccttatgattc ttattaattc 180 cagtctatat catctatatg tactcaataa ttgtatggga220 36 58 PRT NICOTIANATABACUM 36 Met Leu Phe Gly Leu Ala Asn Val GlyGln Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Leu ProAsn Gly Gln Ser His Glu Asn Phe 20 25 30 Asp Met Thr Glu Ser Pro Gly IleSer Ala Thr Arg Lys Asp Asp Leu 35 40 45 Val Leu Ile Ala Thr Pro Tyr AspSer Tyr 50 55 37 174 DNA NICOTIANATABACUM 37 atgctatttg gtttagctaatgttggacaa cctttagctc agttacttta tcacttcgat 60 tggaaactcc ctaatggacaaactcaccaa aatttcgaca tgactgagtc acctggaatt 120 tctgctacaa gaaaggatgatcttattttg attgccactc ctgctcattc ttga 174 38 57 PRT NICOTIANATABACUM 38Met Leu Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu 1 5 1015 Tyr His Phe Asp Trp Lys Leu Pro Asn Gly Gln Thr His Gln Asn Phe 20 2530 Asp Met Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu 35 4045 Ile Leu Ile Ala Thr Pro Ala His Ser 50 55 39 177 DNA NICOTIANATABACUM39 ttattattcg gtttagttaa tgtaggacat cctttagctc aattgcttta tcacttcgat 60tggaagactc ttcctgggat aagttcagat agtttcgaca tgactgaaac agatggagta 120actgccggaa gaaaggatga tctttgttta attgctactc cttttggtct caattaa 177 40 58PRT NICOTIANATABACUM 40 Leu Leu Phe Gly Leu Val Asn Val Gly His Pro LeuAla Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Thr Leu Pro Gly IleSer Ser Asp Ser Phe 20 25 30 Asp Met Thr Glu Thr Asp Gly Val Thr Ala GlyArg Lys Asp Asp Leu 35 40 45 Cys Leu Ile Ala Thr Pro Phe Gly Leu Asn 5055 41 180 DNA NICOTIANATABACUM 41 atgtcgtttg gtttagttaa cactgggcatcctttagctc agttgctcta tttctttgac 60 tggaaattcc ctcataaggt taatgcagctgattttcaca ctactgaaac aagtagagtt 120 tttgcagcaa gcaaagatga cctctacttgattccaacaa atcacatgga gcaagagtag 180 42 59 PRT NICOTIANATABACUM 42 MetSer Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu 1 5 10 15Tyr Phe Phe Asp Trp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe 20 25 30His Thr Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu 35 40 45Tyr Leu Ile Pro Thr Asn His Met Glu Gln Glu 50 55 43 180 DNANICOTIANATABACUM 43 atgtcgtttg gtttagttaa cacagggcat cctttagcccagttgctcta ttgctttgac 60 tggaaactcc ctgacaaggt taatgcaaat gattttcgcactactgaaac aagtagagtt 120 tttgcagcaa gcaaagatga cctctacttg attcccacaaatcacaggga gcaagaatag 180 44 59 PRT NICOTIANATABACUM 44 Met Ser Phe GlyLeu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr Cys PheAsp Trp Lys Leu Pro Asp Lys Val Asn Ala Asn Asp Phe 20 25 30 Arg Thr ThrGlu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu 35 40 45 Tyr Leu IlePro Thr Asn His Arg Glu Gln Glu 50 55 45 183 DNA NICOTIANATABACUM 45atgcaatttg gtttggctct tgttactctg ccattggctc atttgcttca caattttgat 60tggaaacttc ccgaaggaat taatgcaagg gatttggaca tgacagaggc aaatgggata 120tctgctagaa gagaaaaaga tctttacttg attgctactc cttatgtatc acctcttgat 180taa 183 46 60 PRT NICOTIANATABACUM 46 Met Gln Phe Gly Leu Ala Leu ValThr Leu Pro Leu Ala His Leu Leu 1 5 10 15 His Asn Phe Asp Trp Lys LeuPro Glu Gly Ile Asn Ala Arg Asp Leu 20 25 30 Asp Met Thr Glu Ala Asn GlyIle Ser Ala Arg Arg Glu Lys Asp Leu 35 40 45 Tyr Leu Ile Ala Thr Pro TyrVal Ser Pro Leu Asp 50 55 60 47 177 DNA NICOTIANATABACUM 47 atgacttatgcattgcaagt ggaacaccta acaatggcac atttgatcca gggtttcaat 60 tacagaactccaactgatga gcccttggat atgaaagaag gtgcaggcat aactatacgt 120 aaggtaaatcctgtgaaagt gataattacg cctcgcttgg cacctgagct ttattaa 177 48 58 PRTNICOTIANATABACUM 48 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met AlaHis Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Arg Thr Pro Thr Asp Glu ProLeu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn ProVal Lys Val Ile 35 40 45 Ile Thr Pro Arg Leu Ala Pro Glu Leu Tyr 50 5549 177 DNA NICOTIANATABACUM 49 atgacttatg cattgcaagt ggaacacctaacaatggcac atttaatcca gggtttcaat 60 tacaaaactc caaatgacga ggccttggatatgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgtggaact gataatagcgcctcgcctgg cacctgagct ttattaa 177 50 58 PRT NICOTIANATABACUM 50 Met ThrTyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 GlnGly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys 20 25 30 GluGly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile 35 40 45 IleAla Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 51 177 DNA NICOTIANATABACUM 51atgacttatg cattgcaagt ggaacaccta acaatggcac atttaatcca gggtttcaat 60tacaaaactc caaatgacga ggccttggat atgaaggaag gtgcaggcat aacaatacgt 120aaggtaaatc cagtggaatt gataataacg cctcgcttgg cacctgagct ttactaa 177 52 58PRT NICOTIANATABACUM 52 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr MetAla His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp GluAla Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val AsnPro Val Glu Leu Ile 35 40 45 Ile Thr Pro Arg Leu Ala Pro Glu Leu Tyr 5055 53 177 DNA NICOTIANATABACUM 53 atgacttatg cattgcaagt ggaacacttaacaatggcac atttgatcca aggtttcaat 60 tacagaactc caaatgacga gcccttggatatgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgtggaact gataatagcgcctcgcctgg cacctgagct ttattaa 177 54 58 PRT NICOTIANATABACUM 54 Met ThrTyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 GlnGly Phe Asn Tyr Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 GluGly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile 35 40 45 IleAla Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 55 174 DNA NICOTIANATABACUM 55atgacttatg cattgcaagt ggaacactta acaatggcac atttgatcca aggtttcaat 60tacagaactc caaatgacga gcccttggat atgaaggaag gtgcaggcat aactatacgt 120aaggtaaatc ctgtggaact gataatagcg cccctggcac ctgagcttta ttaa 174 56 57PRT NICOTIANATABACUM 56 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr MetAla His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Arg Thr Pro Asn Asp GluPro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val AsnPro Val Glu Leu Ile 35 40 45 Ile Ala Pro Leu Ala Pro Glu Leu Tyr 50 5557 177 DNA NICOTIANATABACUM 57 atgacttatg cattgcaagt ggaacacttaacaatggcac atttgatcca aggtttcaat 60 tacagaactc caaatgacga gcccttggatatgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgcggaact gataatagcgcctcgcctgg cacctgagct ttattaa 177 58 58 PRT NICOTIANATABACUM 58 Met ThrTyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 GlnGly Phe Asn Tyr Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 GluGly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Ala Glu Leu Ile 35 40 45 IleAla Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 59 177 DNA NICOTIANATABACUM 59atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca gggtttcaat 60tacaaaactc caaatgacga gcccttggat atgaaggaag gtgcaggatt aaccatacgt 120aaagtaaatc ctgtagaagt gacaactacg gctcgcctgg cacctgagct ttattaa 177 60 58PRT NICOTIANATABACUM 60 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr IleAla His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp GluPro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Leu Thr Ile Arg Lys Val AsnPro Val Glu Val Thr 35 40 45 Thr Thr Ala Arg Leu Ala Pro Glu Leu Tyr 5055 61 177 DNA NICOTIANATABACUM 61 atgacttatg cattacaagt ggaacacctaacaatagcac atttgatcca gggtttcaat 60 tacaaaactc caaatgacga gcccttggatatgaaggaag gtgcaggatt aactatacgt 120 aaagtaaatc ctgtagaagt gacaattacggctcgcctgg cacctgagct ttattaa 177 62 58 PRT NICOTIANATABACUM 62 Met ThrTyr Ala Leu Gln Val Glu His Leu Thr Ile Ala His Leu Ile 1 5 10 15 GlnGly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 GluGly Ala Gly Leu Thr Ile Arg Lys Val Asn Pro Val Glu Val Thr 35 40 45 IleThr Ala Arg Leu Ala Pro Glu Leu Tyr 50 55 63 177 DNA NICOTIANATABACUM 63atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca gggtttcaat 60tacaaaactc caaatgacga gcccctggat atgaaggaag gtgcaggatt aactatacgt 120aaagtaaatc ctgtagaagt gacaattacg gctcgcctgg cacctgagct ttattaa 177 64 58PRT NICOTIANATABACUM 64 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr IleAla His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp GluPro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Leu Thr Ile Arg Lys Val AsnPro Val Glu Val Thr 35 40 45 Ile Thr Ala Arg Leu Ala Pro Glu Leu Tyr 5055 65 198 DNA NICOTIANATABACUM 65 tatagccttg gacttaaggt tatccgagtaacattagcca acatgttgca tggattcaac 60 tggaaattac ctgaaggtat gaagccagaagatataagtg tggaagaaca ttatgggctc 120 actacacatc ctaagtttcc tgttcctgtgatcttggaat ctagactttc ttcagatctc 180 tattccccca tcacttaa 198 66 65 PRTNICOTIANATABACUM 66 Tyr Ser Leu Gly Leu Lys Val Ile Arg Val Thr Leu AlaAsn Met Leu 1 5 10 15 His Gly Phe Asn Trp Lys Leu Pro Glu Gly Met LysPro Glu Asp Ile 20 25 30 Ser Val Glu Glu His Tyr Gly Leu Thr Thr His ProLys Phe Pro Val 35 40 45 Pro Val Ile Leu Glu Ser Arg Leu Ser Ser Asp LeuTyr Ser Pro Ile 50 55 60 Thr 65 67 190 DNA NICOTIANATABACUM 67atacagtctt gggattcgta taattagggc aactttagct aacttgttgc atggattcaa 60ctggagattg cctaatggta tgagtccaga agacattagc atggaagaga tttatgggct 120aattacacac cccaaagtcg cacttgacgt gatgatggag cctcgacttc ccaaccatct 180ttacaaatag 190 68 62 PRT NICOTIANATABACUM 68 Tyr Ser Leu Gly Ile Arg IleIle Arg Ala Thr Leu Ala Asn Leu Leu 1 5 10 15 His Gly Phe Asn Trp ArgLeu Pro Asn Gly Met Ser Pro Glu Asp Ile 20 25 30 Ser Met Glu Glu Ile TyrGly Leu Ile Thr His Pro Lys Val Ala Leu 35 40 45 Asp Val Met Met Glu ProArg Leu Pro Asn His Leu Tyr Lys 50 55 60 69 174 DNA NICOTIANATABACUM 69attaattttt caataccact tgttgagctt gcacttgcta atctattgtt tcattataat 60tggtcacttc ctgaagggat gctagctaag gatgttgata tggaagaagc tttggggatt 120accatgcaca agaaatctcc cctttgctta gtagcttctc attatacttg ttga 174 70 57PRT NICOTIANATABACUM 70 Ile Asn Phe Ser Ile Pro Leu Val Glu Leu Ala LeuAla Asn Leu Leu 1 5 10 15 Phe His Tyr Asn Trp Ser Leu Pro Glu Gly MetLeu Ala Lys Asp Val 20 25 30 Asp Met Glu Glu Ala Leu Gly Ile Thr Met HisLys Lys Ser Pro Leu 35 40 45 Cys Leu Val Ala Ser His Tyr Thr Cys 50 5571 190 DNA NICOTIANATABACUM 71 atgcaacttg ggctttatgc attggaaatggctgtggccc atcttcttca ttgttttact 60 tgggaattgc cagatggtat gaaaccaagtgagcttaaaa tggatgatat ttttggactc 120 actgctccaa aagctaatcg actcgtggctgtgcctactc cacgtttgtt gtgtcccctt 180 tattaattga 190 72 61 PRTNICOTIANATABACUM 72 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val AlaHis Leu Leu 1 5 10 15 His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met LysPro Ser Glu Leu 20 25 30 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro LysAla Asn Arg Leu 35 40 45 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro LeuTyr 50 55 60 73 186 DNA NICOTIANATABACUM 73 atgcaacttg ggctttatgcattagaaatg gcagtggccc atcttcttct ttgctttact 60 tgggaattgc cagatggtatgaaaccaagt gagcttaaaa tggatgatat ttttggactc 120 actgctccaa gagctaatcgactcgtggct gtgcctagtc cacgtttgtt gtgcccactt 180 tattaa 186 74 61 PRTNICOTIANATABACUM 74 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val AlaHis Leu Leu 1 5 10 15 Leu Cys Phe Thr Trp Glu Leu Pro Asp Gly Met LysPro Ser Glu Leu 20 25 30 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro ArgAla Asn Arg Leu 35 40 45 Val Ala Val Pro Ser Pro Arg Leu Leu Cys Pro LeuTyr 50 55 60 75 186 DNA NICOTIANATABACUM 75 atgcaacttg ggctttatgcattggaaatg gctgtggccc atcttcttca ttgttttact 60 tgggaattgc cagatggtatgaaaccaagt gagcttaaaa tggatgatat ttttggactc 120 actgctccaa gagctaatcgactcgtggct gtgcctactc cacgtttgtt gtgtcccctt 180 tattaa 186 76 61 PRTNICOTIANATABACUM 76 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val AlaHis Leu Leu 1 5 10 15 His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met LysPro Ser Glu Leu 20 25 30 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro ArgAla Asn Arg Leu 35 40 45 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro LeuTyr 50 55 60 77 93 DNA NICOTIANATABACUM 77 atgctttgga gtgcgagtatagtgcgcgtc agctacctaa cttgtattta tagattccaa 60 gtatatgctg ggtctgtgttcagagtagca tga 93 78 30 PRT NICOTIANATABACUM 78 Met Leu Trp Ser Ala SerIle Val Arg Val Ser Tyr Leu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln ValTyr Ala Gly Ser Val Phe Arg Val Ala 20 25 30 79 93 DNA NICOTIANATABACUM79 atgctttgga gtgcgagtat agtgcgcgtc agctacctaa cttgtattta tagattccaa 60gtatatgctg ggtctgtgtt cagagtagca tga 93 80 30 PRT NICOTIANATABACUM 80Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser Tyr Leu Thr Cys Ile 1 5 1015 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val Phe Arg Val Ala 20 25 30 8193 DNA NICOTIANATABACUM 81 atgctttgga gtgcgagtat agtgcgcgtc agctacctaacctgtattta tagattccaa 60 gtatatgctg ggtctgtgtt cagagtagca tga 93 82 30PRT NICOTIANATABACUM 82 Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser TyrLeu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val PheArg Val Ala 20 25 30 83 177 DNA NICOTIANATABACUM 83 attaatttttcaataccact tgttgagctt gcacttgcta atctattgtt tcattataat 60 tggtcacttcctgaggggat gctacctaag gatgttgata tggaagaagc tttggggatt 120 accatgcacaagaaatctcc cctttgctta gtagcttctc attataactt gttgtga 177 84 58 PRTNICOTIANATABACUM 84 Ile Asn Phe Ser Ile Pro Leu Val Glu Leu Ala Leu AlaAsn Leu Leu 1 5 10 15 Phe His Tyr Asn Trp Ser Leu Pro Glu Gly Met LeuPro Lys Asp Val 20 25 30 Asp Met Glu Glu Ala Leu Gly Ile Thr Met His LysLys Ser Pro Leu 35 40 45 Cys Leu Val Ala Ser His Tyr Asn Leu Leu 50 5585 183 DNA NICOTIANATABACUM 85 atatcatttg gtttggctaa tgtttatttgccactagctc aattgttata tcattttgat 60 tggaaactcc ctactggaat caattcaagtgacttggaca tgactgagtc gtcaggagta 120 acttgtgcta gaaagagtga tttatacttgactgctactc catatcaact ttctcaagag 180 tga 183 86 61 PRT NICOTIANATABACUM86 Gly Ile Ser Phe Gly Leu Ala Asn Val Tyr Leu Pro Leu Ala Gln Leu 1 510 15 Leu Tyr His Phe Asp Trp Lys Leu Pro Thr Gly Ile Asn Ser Ser Asp 2025 30 Leu Asp Met Thr Glu Ser Ser Gly Val Thr Cys Ala Arg Lys Ser Asp 3540 45 Leu Tyr Leu Thr Ala Thr Pro Tyr Gln Leu Ser Gln Glu 50 55 60 87 93DNA NICOTIANATABACUM 87 atgctttgga gtgcgagtat agtgcgcgtc agctacctaacttgtattta tagattccaa 60 gtatatgctg ggtctgtgtc cagagtagca tga 93 88 30PRT NICOTIANATABACUM 88 Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser TyrLeu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val SerArg Val Ala 20 25 30 89 153 DNA NICOTIANATABACUM 89 ctgaattttgcaatgttaga ggcaaaaatg gcacttgcat tgattctaca acactatgct 60 tttgagctctctccatctta tgcacatgct cctcatacaa ttatcactct gcaacctcaa 120 catggtgctcctttgatttt gcgcaagctg tag 153 90 50 PRT NICOTIANATABACUM 90 Leu Asn PheAla Met Leu Glu Ala Lys Met Ala Leu Ala Leu Ile Leu 1 5 10 15 Gln HisTyr Ala Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro His 20 25 30 Thr IleIle Thr Leu Gln Pro Gln His Gly Ala Pro Leu Ile Leu Arg 35 40 45 Lys Leu50 91 153 DNA NICOTIANATABACUM 91 ctgaattttg caatgttaga ggcaaaaatggcacttgcat tgattctaca acactatgct 60 tttgagctct ctccatctta tgcacacgctcctcatacaa ttatcactct gcaacctcaa 120 catggtgctc ctttgatttt gcgcaagctgtag 153 92 50 PRT NICOTIANATABACUM 92 Leu Asn Phe Ala Met Leu Glu AlaLys Met Ala Leu Ala Leu Ile Leu 1 5 10 15 Gln His Tyr Ala Phe Glu LeuSer Pro Ser Tyr Ala His Ala Pro His 20 25 30 Thr Ile Ile Thr Leu Gln ProGln His Gly Ala Pro Leu Ile Leu Arg 35 40 45 Lys Leu 50 93 226 DNANICOTIANATABACUM 93 aataattttg caatgttgga aactaagatt gccttagcaatgatcctaca gcgttttgct 60 ttcgagcttt ctccatctta cgctcatgca cctacttatgtcgtcactct tcgacctcag 120 tgtggtgctc acttaatctt gcaaaaatta taggtccttaatctggattt cccattattg 180 agtagtgcct aataaatctt ctctatcact atttttccatctttca 226 94 50 PRT NICOTIANATABACUM 94 Asn Asn Phe Ala Met Leu Glu ThrLys Ile Ala Leu Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ala Phe Glu LeuSer Pro Ser Tyr Ala His Ala Pro Thr 20 25 30 Tyr Val Val Thr Leu Arg ProGln Cys Gly Ala His Leu Ile Leu Gln 35 40 45 Lys Leu 50 95 268 DNANICOTIANATABACUM 95 agcgaagggg tggcaaaggc aacaaagggg aaaatgacatattttccatt tggtgcagga 60 ccgcgaaaat gcattgggca aaacttcgcg attttggaagcaaaaatggc tatagctatg 120 attctacaac gcttctcctt cgagctctcc ccatcttatacacactctcc atacactgtg 180 gtcactttga aacccaaata tggtgctccc ctaataatgcacaggctgta gtcctgtgag 240 aatatgctat ccgaggaatt cagttcct 268 96 50 PRTNICOTIANATABACUM 96 Gln Asn Phe Ala Ile Leu Glu Ala Lys Met Ala Ile AlaMet Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr ThrHis Ser Pro Tyr 20 25 30 Thr Val Val Thr Leu Lys Pro Lys Tyr Gly Ala ProLeu Ile Met His 35 40 45 Arg Leu 50 97 237 DNA NICOTIANATABACUM 97agcgaagggg tggcaaaggc aacaaagggg aaaatgacat attttccatt tggtgcagga 60ccgcgaaaat gcattgggca aaacttcgcg attttggaag caaaaatggc tatagctatg 120attctacaac gcttctcctt cgagctctct ccatcttata cacactctcc atacactgtg 180gtcactttga aacccaaata tggtgctccc ctaataatgc acaggctgta gtcctgt 237 98 50PRT NICOTIANATABACUM 98 Gln Asn Phe Ala Ile Leu Glu Ala Lys Met Ala IleAla Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser TyrThr His Ser Pro Tyr 20 25 30 Thr Val Val Thr Leu Lys Pro Lys Tyr Gly AlaPro Leu Ile Met His 35 40 45 Arg Leu 50 99 152 DNA NICOTIANATABACUM 99caaaattttg ccatgttaga agcaaagatg gctctgtcta tgatcctgca acgcttctct 60tttgaactgt ctccgtctta tgcacatgcc cctcagtcca tattaaccgt cagccacaat 120atggtgctcc acttattttc cacaagctat aa 152 100 50 PRT NICOTIANATABACUM 100Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala Leu Ser Met Ile Leu 1 5 1015 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Gln 20 2530 Ser Ile Leu Thr Val Gln Pro Gln Tyr Gly Ala Pro Leu Ile Phe His 35 4045 Lys Leu 50 101 153 DNA NICOTIANATABACUM 101 ataaactttg caatgacagaagcgaagatg gctatggcta tgattctgca acgcttctcc 60 tttgagctat ctccatcttacacacatgct ccacagtctg taataactat gcaaccccaa 120 tatggtgctc ctcttatattgcacaaattg taa 153 102 50 PRT NICOTIANATABACUM 102 Ile Asn Phe Ala MetThr Glu Ala Lys Met Ala Met Ala Met Ile Leu 1 5 10 15 Gln Arg Phe SerPhe Glu Leu Ser Pro Ser Tyr Thr His Ala Pro Gln 20 25 30 Ser Val Ile ThrMet Gln Pro Gln Tyr Gly Ala Pro Leu Ile Leu His 35 40 45 Lys Leu 50 103153 DNA NICOTIANATABACUM 103 ataaactttg caatggcaga agcgaagatg gctatggctatgattctgca acgcttctcc 60 tttgagctat ctccatctta cacacatgct ccacagtctgtaataactat gcaaccccaa 120 tatggtgctc ctcttatatt gcacaaattg taa 153 10450 PRT NICOTIANATABACUM 104 Ile Asn Phe Ala Met Ala Glu Ala Lys Met AlaMet Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro SerTyr Thr His Ala Pro Gln 20 25 30 Ser Val Ile Thr Met Gln Pro Gln Tyr GlyAla Pro Leu Ile Leu His 35 40 45 Lys Leu 50 105 153 DNA NICOTIANATABACUM105 caaaactttg caatgatgga agcaaaaatg gcagtagcta tgatactaca aaaattttcc 60tttgaactat ccccttctta tacacatgct ccatttgcaa ttgtgactat tcatcctcag 120tatggtgctc ctctgcttat gcgcagactt taa 153 106 50 PRT NICOTIANATABACUM 106Gln Asn Phe Ala Met Met Glu Ala Lys Met Ala Val Ala Met Ile Leu 1 5 1015 Gln Lys Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ala Pro Phe 20 2530 Ala Ile Val Thr Ile His Pro Gln Tyr Gly Ala Pro Leu Leu Met Arg 35 4045 Arg Leu 50 107 153 DNA NICOTIANATABACUM 107 caaaactttg caatgatggaagcaaaaatg gcagtagcta tgatactaca taaattttcc 60 tttgaactat ccccttcttatacacatgct ccatttgcaa ttgtgactat tcatcctcag 120 tatggtgctc ctctgcttatgcgcagactt taa 153 108 49 PRT NICOTIANATABACUM 108 Gln Asn Phe Ala MetMet Glu Ala Lys Met Ala Val Ala Met Ile Leu 1 5 10 15 His Lys Phe SerPhe Glu Leu Pro Ser Tyr Thr His Ala Pro Phe Ala 20 25 30 Ile Val Thr IleHis Pro Gln Tyr Gly Ala Pro Leu Leu Met Arg Arg 35 40 45 Leu 109 150 DNANICOTIANATABACUM 109 caaaattttg ctatgttaga ggctaaaatg gcaatggctatgattctgaa aacctatgca 60 tttgaactct ctccatctta tgctcatgct cctcatccactactacttca acctcaatat 120 ggtgctcaat taattttgta caagttgtag 150 110 49PRT NICOTIANATABACUM 110 Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala MetAla Met Ile Leu 1 5 10 15 Lys Thr Tyr Ala Phe Glu Leu Ser Pro Ser TyrAla His Ala Pro His 20 25 30 Pro Leu Leu Leu Gln Pro Gln Tyr Gly Ala GlnLeu Ile Leu Tyr Lys 35 40 45 Leu 111 192 DNA NICOTIANATABACUM 111tatagcatgg ggctcaaggc gattcaagct agcttagcta atcttctaca tggatttaac 60tggtcattgc ctgataatat gactcctgag gacctcaaca tggatgagat ttttgggctc 120tctacaccta aaaaatttcc acttgctact gtgattgagc caagactttc accaaaactt 180tactctgttt ga 192 112 63 PRT NICOTIANATABACUM 112 Tyr Ser Met Gly LeuLys Ala Ile Gln Ala Ser Leu Ala Asn Leu Leu 1 5 10 15 His Gly Phe AsnTrp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu 20 25 30 Asn Met Asp GluIle Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu 35 40 45 Ala Thr Val IleGlu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 50 55 60 113 192 DNANICOTIANATABACUM 113 tatagcttgg ggctcaagga gattcaagct agcttagctaatcttctaca tggatttaac 60 tggtcattgc ctgataatat gactcctgag gacctcaacatggatgagat ttttgggctc 120 tctacaccta aaaaatttcc acttgctact gtgattgagccaagactttc accaaaactt 180 tactctgttt ga 192 114 63 PRT NICOTIANATABACUM114 Tyr Ser Leu Gly Leu Lys Glu Ile Gln Ala Ser Leu Ala Asn Leu Leu 1 510 15 His Gly Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu 2025 30 Asn Met Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu 3540 45 Ala Thr Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 50 5560 115 192 DNA NICOTIANATABACUM 115 catagcttgg ggctcaaggt gattcaagctagcttagcta atcttctaca tggatttaac 60 tggtcattgc ctgataatat gactcctgaggacctcaaca tggatgagat ttttgggctc 120 tctacaccta aaaaatttcc acttgctactgtgattgagc caagactttc accaaaactt 180 tactctgttt ga 192 116 63 PRTNICOTIANATABACUM 116 His Ser Leu Gly Leu Lys Val Ile Gln Ala Ser Leu AlaAsn Leu Leu 1 5 10 15 His Gly Phe Asn Trp Ser Leu Pro Asp Asn Met ThrPro Glu Asp Leu 20 25 30 Asn Met Asp Glu Ile Phe Gly Leu Ser Thr Pro LysLys Phe Pro Leu 35 40 45 Ala Thr Val Ile Glu Pro Arg Leu Ser Pro Lys LeuTyr Ser Val 50 55 60 117 96 DNA NICOTIANATABACUM 117 ctgtgctttccatgtttaat ctctagttat atactggctt tgaatgtgaa tctgtatcat 60 aatttcttgcaaatttctcc ttccatttct tattaa 96 118 31 PRT NICOTIANATABACUM 118 Leu CysPhe Pro Cys Leu Ile Ser Ser Tyr Ile Leu Ala Leu Asn Val 1 5 10 15 AsnLeu Tyr His Asn Phe Leu Gln Ile Ser Pro Ser Ile Ser Tyr 20 25 30 119 192DNA NICOTIANATABACUM 119 tctggacttg ctcaatgtgt ggttggttta gctttagcaactctagtgca gtgttttgag 60 tggaaaaggg taagcgaaga ggtggttgat ttgacggaaggaaaaggtct cactatgcca 120 aaacccgagc cactcatggc taggtgcgaa gctcgtgacatttttcacaa agttctttca 180 gaaatatctt aa 192 120 63 PRT NICOTIANATABACUM120 Ser Gly Leu Ala Gln Cys Val Val Gly Leu Ala Leu Ala Thr Leu Val 1 510 15 Gln Cys Phe Glu Trp Lys Arg Val Ser Glu Glu Val Val Asp Leu Thr 2025 30 Glu Gly Lys Gly Leu Thr Met Pro Lys Pro Glu Pro Leu Met Ala Arg 3540 45 Cys Glu Ala Arg Asp Ile Phe His Lys Val Leu Ser Glu Ile Ser 50 5560 121 174 DNA NICOTIANATABACUM 121 ttgggcttgg caacggtgca tgtgaatttgatgttggccc gaatgattca agaatttgaa 60 tggtccgctt acccggaaaa taggaaagtggattttactg agaaattgga atttactgtg 120 gtgatgaaaa atcctttaag agctaaggtcaagccaagaa tgcaagtggt gtaa 174 122 57 PRT NICOTIANATABACUM 122 Leu GlyLeu Ala Thr Val His Val Asn Leu Met Leu Ala Arg Met Ile 1 5 10 15 GlnGlu Phe Glu Trp Ser Ala Tyr Pro Glu Asn Arg Lys Val Asp Phe 20 25 30 ThrGlu Lys Leu Glu Phe Thr Val Val Met Lys Asn Pro Leu Arg Ala 35 40 45 LysVal Lys Pro Arg Met Gln Val Val 50 55 123 171 DNA NICOTIANATABACUM 123tatgctttgg ctatgcttca tttagagtac tttgtggcta atttggtttg gcattttcga 60tgggaggctg tggagggaga tgatgttgat ctttcagaaa agctagaatt caccgttgtg 120atgaagaatc cacttcgagc tcgtatctgc cccagagtta actctatttg a 171 124 56 PRTNICOTIANATABACUM 124 Tyr Ala Leu Ala Met Leu His Leu Glu Tyr Phe Val AlaAsn Leu Val 1 5 10 15 Trp His Phe Arg Trp Glu Ala Val Glu Gly Asp AspVal Asp Leu Ser 20 25 30 Glu Lys Leu Glu Phe Thr Val Val Met Lys Asn ProLeu Arg Ala Arg 35 40 45 Ile Cys Pro Arg Val Asn Ser Ile 50 55 125 186DNA NICOTIANATABACUM 125 ggtcagcaag ttggacttct tagaacaacc attttcatcgcctcattact gtctgaatat 60 aagctgaaac ctcgctcaca ccagaaacaa gttgaactcaccgatttaaa tccagcaagt 120 tggcttcatt cgataaaagg cgaactgtta gtcgatgcgattcctcgaaa gaaggcggca 180 ttttaa 186 126 61 PRT NICOTIANATABACUM 126 GlyGln Gln Val Gly Leu Leu Arg Thr Thr Ile Phe Ile Ala Ser Leu 1 5 10 15Leu Ser Glu Tyr Lys Leu Lys Pro Arg Ser His Gln Lys Gln Val Glu 20 25 30Leu Thr Asp Leu Asn Pro Ala Ser Trp Leu His Ser Ile Lys Gly Glu 35 40 45Leu Leu Val Asp Ala Ile Pro Arg Lys Lys Ala Ala Phe 50 55 60 127 171 DNANICOTIANATABACUM 127 atcacatttg ctaagtttgt gaatgagcta gcattggcaagattaatgtt ccattttgat 60 ttctcgctac caaaaggagt taagcatgag gatttggacgtggaggaagc tgctggaatt 120 actgttagaa ggaagttccc ccttttagcc gtcgccactccatgctcgtg a 171 128 56 PRT NICOTIANATABACUM 128 Ile Thr Phe Ala Lys PheVal Asn Glu Leu Ala Leu Ala Arg Leu Met 1 5 10 15 Phe His Phe Asp PheSer Leu Pro Lys Gly Val Lys His Glu Asp Leu 20 25 30 Asp Val Glu Glu AlaAla Gly Ile Thr Val Arg Arg Lys Phe Pro Leu 35 40 45 Leu Ala Val Ala ThrPro Cys Ser 50 55 129 180 DNA NICOTIANATABACUM 129 cagaggtatg ctataaaccatttgatgctc tttattgcgt tgttcacggc tctgattgat 60 ttcaagaggc acaaaacggacggctgtgat gatatcgcgt atattccaac cattgctcca 120 aaggatgatt gtaaagtgttcctttcacag aggtgcactc gattcccatc tttttcatga 180 130 59 PRTNICOTIANATABACUM 130 Gln Arg Tyr Ala Ile Asn His Leu Met Leu Phe Ile AlaLeu Phe Thr 1 5 10 15 Ala Leu Ile Asp Phe Lys Arg His Lys Thr Asp GlyCys Asp Asp Ile 20 25 30 Ala Tyr Ile Pro Thr Ile Ala Pro Lys Asp Asp CysLys Val Phe Leu 35 40 45 Ser Gln Arg Cys Thr Arg Phe Pro Ser Phe Ser 5055 131 183 DNA NICOTIANATABACUM 131 atgtcatttg gtttagctaa tctttacttaccattggctc aattactcta tcactttgac 60 tggaaactcc caaccggaat caagccaagagacttggact tgaccgaatt atcgggaata 120 actattgcta gaaagggtga cctttacttaaatgctactc cttatcaacc ttctcgagag 180 taa 183 132 60 PRT NICOTIANATABACUM132 Met Ser Phe Gly Leu Ala Asn Leu Tyr Leu Pro Leu Ala Gln Leu Leu 1 510 15 Tyr His Phe Asp Trp Lys Leu Pro Thr Gly Ile Lys Pro Arg Asp Leu 2025 30 Asp Leu Thr Glu Leu Ser Gly Ile Thr Ile Ala Arg Lys Gly Asp Leu 3540 45 Tyr Leu Asn Ala Thr Pro Tyr Gln Pro Ser Arg Glu 50 55 60 133 174DNA NICOTIANATABACUM 133 ttgggcttgg caacggtgca tgtgaatttg atgttggcccgaacgattca agaatttgaa 60 tggtccgctt acccggaaaa taggaaagtg gattttactgagaaattgga atttactgtg 120 gtgatgaaaa accctttaag agctaaggtc aagccaagaatgcaagtggt gtaa 174 134 57 PRT NICOTIANATABACUM 134 Leu Gly Leu Ala ThrVal His Val Asn Leu Met Leu Ala Arg Thr Ile 1 5 10 15 Gln Glu Phe GluTrp Ser Ala Tyr Pro Glu Asn Arg Lys Val Asp Phe 20 25 30 Thr Glu Lys LeuGlu Phe Thr Val Val Met Lys Asn Pro Leu Arg Ala 35 40 45 Lys Val Lys ProArg Met Gln Val Val 50 55 135 123 DNA NICOTIANATABACUM 135 ttgggcttggcaacggtgca tgtgaatttg atgttggccc gaatgattca agaatttgaa 60 tggtccgcttacccggaaaa taggaaagtg gatttactga gaaattggaa tttactgtgg 120 tga 123 13640 PRT NICOTIANATABACUM 136 Leu Gly Leu Ala Thr Val His Val Asn Leu MetLeu Ala Arg Met Ile 1 5 10 15 Gln Glu Phe Glu Trp Ser Ala Tyr Pro GluAsn Arg Lys Val Asp Leu 20 25 30 Leu Arg Asn Trp Asn Leu Leu Trp 35 40137 171 DNA NICOTIANATABACUM 137 atcacatttg ctaagtttgt gaatgagctagcattggcaa gattaatgtt ccattttgat 60 ttctcgctac caaaaggagt taagcatgaggatttggacg tggaggaagc tgctggaatt 120 actgttagga ggaagttccc ccttttagccgtcgccactc catgctcgtg a 171 138 56 PRT NICOTIANATABACUM 138 Ile Thr PheAla Lys Phe Val Asn Glu Leu Ala Leu Ala Arg Leu Met 1 5 10 15 Phe HisPhe Asp Phe Ser Leu Pro Lys Gly Val Lys His Glu Asp Leu 20 25 30 Asp ValGlu Glu Ala Ala Gly Ile Thr Val Arg Arg Lys Phe Pro Leu 35 40 45 Leu AlaVal Ala Thr Pro Cys Ser 50 55 139 171 DNA NICOTIANATABACUM 139atcacatttg ctaagtttgt gaatgagcta gcattggcaa gattaatgtt ccattttgat 60ttctcgctac caaaaggagt taagcatgcg gatttggacg tggaggaagc tgctggaatt 120actgttagaa ggaagttccc ccttttagcc gtcgccactc catgctcgtg a 171 140 56 PRTNICOTIANATABACUM 140 Ile Thr Phe Ala Lys Phe Val Asn Glu Leu Ala Leu AlaArg Leu Met 1 5 10 15 Phe His Phe Asp Phe Ser Leu Pro Lys Gly Val LysHis Ala Asp Leu 20 25 30 Asp Val Glu Glu Ala Ala Gly Ile Thr Val Arg ArgLys Phe Pro Leu 35 40 45 Leu Ala Val Ala Thr Pro Cys Ser 50 55 141 153DNA NICOTIANATABACUM 141 caaaattttg ccatgttaga agcaaagact actttggctatgatcctaca acgcttctcc 60 tttgaactgt ctccatctta tgcacatgct cctcagtccataataacttt gcaaccccag 120 tatggtgctc cacttatttt gcataaaata tag 153 14250 PRT NICOTIANATABACUM 142 Gln Asn Phe Ala Met Leu Glu Ala Lys Thr ThrLeu Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro SerTyr Ala His Ala Pro Gln 20 25 30 Ser Ile Ile Thr Leu Gln Pro Gln Tyr GlyAla Pro Leu Ile Leu His 35 40 45 Lys Ile 50 143 171 DNA NICOTIANATABACUM143 attatccttg cactgccaat tcttggcatt accttgggac gcttggtgca gaactttgag 60ttgttgcctc ctccaggaca gtcaaagctt gacacaacag agaaaggcgg gcaattcagt 120ctgcacattt tgaagcattc caccattgtg atgaaaccaa gatcttttta a 171 144 56 PRTNICOTIANATABACUM 144 Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu GlyArg Leu Val 1 5 10 15 Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln SerLys Leu Asp Thr 20 25 30 Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile LeuLys His Ser Thr 35 40 45 Ile Val Met Lys Pro Arg Ser Phe 50 55 145 267DNA NICOTIANATABACUM 145 caaaattttg ccatgttaga agcaaagact actttggctatgatcctaca acgcttctcc 60 tttgaactgt ctccatctta tgcacatgct cctcagtccataataacttg caaccccagt 120 atggtgctcc acttattttg cataaaatat agtttattacttgtaagtag tgtctcgttt 180 tatgttaagc atgagtccaa aatgttaagg cttgtagaactgcaaaatgg gaatgcattt 240 gcactcgtgc actgtagatt gttgtaa 267 146 88 PRTNICOTIANATABACUM 146 Gln Asn Phe Ala Met Leu Glu Ala Lys Thr Thr Leu AlaMet Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr AlaHis Ala Pro Gln 20 25 30 Ser Ile Ile Thr Cys Asn Pro Ser Met Val Leu HisLeu Phe Cys Ile 35 40 45 Lys Tyr Ser Leu Leu Leu Val Ser Ser Val Ser PheTyr Val Lys His 50 55 60 Glu Ser Lys Met Leu Arg Leu Val Glu Leu Gln AsnGly Asn Ala Phe 65 70 75 80 Ala Leu Val His Cys Arg Leu Leu 85 147 186DNA NICOTIANATABACUM 147 gctgatatgg ggttgcgagc agtttctttg gcattaggtgcacttattca atgctttgac 60 tggcaaattg aggaagcgga aagcttggag gaaagctataattctagaat gactatgcag 120 aacaagcctt tgaaggttgt ctgcactcca cgcgaagatcttggccagct tctatcccaa 180 ctctaa 186 148 61 PRT NICOTIANATABACUM 148 AlaAsp Met Gly Leu Arg Ala Val Ser Leu Ala Leu Gly Ala Leu Ile 1 5 10 15Gln Cys Phe Asp Trp Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser 20 25 30Tyr Asn Ser Arg Met Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys 35 40 45Thr Pro Arg Glu Asp Leu Gly Gln Leu Leu Ser Gln Leu 50 55 60 149 1576DNA NICOTIANATABACUM 149 cttccttcct aagtcctaac taaaaatgga gattcagttttctaacttag ttgcattctt 60 gctctttctc tccagcatct ttcttctatt caaaaaatggaaaaccagaa aactaaattt 120 gcctcctggt ccatggaaat taccttttat tggaagtttacaccatttgg ctgtggcagg 180 tccacttcct caccatggcc taaaaaattt agccaaacgctatggtcctc ttatgcattt 240 acaacttgga caaattccta cactcatcat atcatcacctcaaatggcaa aagaagtact 300 aaaaactcac gacctcgctt ttgccactag accaaagcttgtcgcggccg acatcattca 360 ctacgacagc acggacatag cattttctcc gtacggtgaatactggagac aaattcgtaa 420 aatttgcata ttggaactct tgagtgccaa gatggtcaaattttttagct cgattcgcca 480 agatgagctc tcgaagatgc tctcatctat acgaacgacacccaatctta cagtcaatct 540 tactgacaaa attttttggt ttacgagttc ggtaacttgtagatcagctt tagggaagat 600 atgtggtgac caagacaaat tgatcatttt tatgagggaaataatatcat tggcaggtgg 660 atttagtatt gctgattttt tccctacatg gaaaatgattcatgatattg atggttcgaa 720 atctaaactg gtgaaagcac atcgtaagat tgatgaaattttgggaaatg ttgttgatga 780 gcacaaaaag aacagagcag atggcaagaa gggtaatggtgaatttggtg gtgaagattt 840 gattgatgta ttgttaagag ttagagaaag tggagaagttcaaattccta tcacaaatga 900 caatatcaaa tcaatattaa tcgacatgtt ctctgcaggatctgaaacat catcgacgac 960 tataatttgg gcattagctg aaatgatgaa gaaaccaagtgttttagcaa aggcacaagc 1020 tgaagtaagg caagctttga aggagaaaaa aggttttcaacagattgatc ttgatgagct 1080 aaaatatctc aagttagtaa tcaaagaaac cttaagaatgcaccctccaa ttcctctatt 1140 agttcctaga gaatgtatgg aggatacaaa gattgatggttacaatatac ctttcaaaac 1200 aagagtcata gttaatgcat gggcaatcgg acgagatccagaaagttggg atgaccccga 1260 aagctttatg ccagagagat ttgagaatag ttctattgactttcttggaa atcatcatca 1320 gtttatacca tttggtgcag gaagaaggat ttgtccgggaatgctatttg gtttagctaa 1380 tgttggacaa cctttagctc agttacttta tcacttcgattggaaactcc ctaatggaca 1440 aagtcatgag aatttcgaca tgactgagtc acctggaatttctgctacaa gaaaggatga 1500 tcttgttttg attgccactc cttatgattc ttattaagcagtagcagaaa taaaaagccg 1560 gggcaaacag aaaaaa 1576 150 503 PRTNICOTIANATABACUM 150 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu LeuPhe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu Phe Lys Lys Trp Lys Thr ArgLys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly SerLeu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu LysAsn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly GlnIle Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser Pro Gln Met Ala Lys Glu ValLeu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val AlaAla Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser ProTyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu GluLeu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg GlnAsp Glu Leu Ser Lys Met Leu Ser 145 150 155 160 Ser Ile Arg Thr Thr ProAsn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr SerSer Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp GlnAsp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala GlyGly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile HisAsp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp Glu His Lys Lys Asn 245 250255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe SerAla 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu AlaGlu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln AlaGlu Val Arg Gln 325 330 335 Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln IleAsp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu Lys Leu Val Ile Lys Glu ThrLeu Arg Met His Pro Pro 355 360 365 Ile Pro Leu Leu Val Pro Arg Glu CysMet Glu Asp Thr Lys Ile Asp 370 375 380 Gly Tyr Asn Ile Pro Phe Lys ThrArg Val Ile Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro GluSer Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410 415 Glu Arg Phe Glu AsnSer Ser Ile Asp Phe Leu Gly Asn His His Gln 420 425 430 Phe Ile Pro PheGly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe 435 440 445 Gly Leu AlaAsn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His Phe 450 455 460 Asp TrpLys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met Thr 465 470 475 480Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu Ile 485 490495 Ala Thr Pro Tyr Asp Ser Tyr 500 151 1581 DNA NICOTIANATABACUM 151tccttcttcc ttcctaagtc ctaactaaaa atggagattc agttttctaa cttagttgca 60ttcttgctct ttctctccag catctttctt ctattcaaaa aatggaaaac cagaaaacta 120aatttgcctc ctggtccatg gaaattacct tttattggaa gtttacacca tttggctgtg 180gcaggtccac ttcctcacca tggcctaaaa aatttagcca aacgctatgg tcctcttatg 240catttacaac ttggacaaat tcctacactc atcatatcat cacctcaaat ggcaaaagaa 300gtactaaaaa ctcacgacct cgcttttgcc actagaccaa agcttgtcgt ggccgacatc 360attcactacg acagcacgga catagcattt tctccgtacg gtgaatactg gagacaaatt 420cgtaaaattt gcatattgga actcttgagt gccaagatgg tcaaattttt tagctcgatt 480cgccaagatg agctctcgaa gatgctctca tctatacgaa cgacacccaa tcttacagtc 540aatcttactg acaaaatttt ttggtttacg agttcggtaa cttgtagatc agctttaggg 600aagatatgtg gtgaccaaga caaattgatc atttttatga gggaaataat atcattggca 660ggtggattta gtattgctga ttttttccct acatggaaaa tgattcatga tattgatggt 720tcgaaatcta aactggtgaa agcacatcgt aagattgatg aaattttggg aaatgttgtt 780gatgagcaca aaaagaacag agcagatggc aagaagggta atggtgaatt tggtggtgaa 840gatttgattg atgtattgtt aagagttaga gaaagtggag aagttcaaat tcctatcaca 900aatgacaata tcaaatcaat attaatcgac atgttctctg cgggatctga aacatcatcg 960acgactataa tttgggcatt agctgaaatg atgaagaaac caagtgtttt agcaaaggca 1020caagctgaag taaggcaagc tttgaaggag aaaaaaggtt ttcaacagat tgatcttgat 1080gagctaaaat atctcaagtt agtaatcaaa gaaaccttaa gaatgcaccc tccaattcct 1140ctattagttc ctagagaatg tatggaggat acaaagattg atggttacaa tatacctttc 1200aaaacaagag tcatagttaa tgcatgggca atcggacgag atccagaaag ttgggatgac 1260cccgaaagct ttatgccaga gagatttgag aatagttcta ttgactttct tggaaatcat 1320catcagttta taccatttgg tgcaggaaga aggatttgtc cgggaatgct atttggttta 1380gctaatgttg gacaaccttt agctcagtta ctttatcact tcgattggaa actccctaat 1440ggacaaagtc atgagaattt cgacatgact gagtcacctg gaatttctgc tacaagaaag 1500gatgatcttg ttttgattgc cactccttat gattcttatt aagcagtagc agaaataaaa 1560agccggggca aacagaaaaa a 1581 152 503 PRT NICOTIANATABACUM 152 Met GluIle Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 SerIle Phe Leu Leu Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 ProPro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 AlaVal Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 ArgTyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80Ile Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met LeuSer 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Thr Val Asn Leu ThrAsp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Thr Cys Arg Ser AlaLeu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe MetArg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp PhePhe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys SerLys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu GlyAsn Val Val Asp Glu His Lys Lys Asn 245 250 255 Arg Ala Asp Gly Lys LysGly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu LeuArg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asn AspAsn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser GluThr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 MetLys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln Ile Asp Leu Asp Glu Leu 340 345350 Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355360 365 Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile Asp370 375 380 Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala TrpAla 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Asp Asp Pro Glu SerPhe Met Pro 405 410 415 Glu Arg Phe Glu Asn Ser Ser Ile Asp Phe Leu GlyAsn His His Gln 420 425 430 Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile CysPro Gly Met Leu Phe 435 440 445 Gly Leu Ala Asn Val Gly Gln Pro Leu AlaGln Leu Leu Tyr His Phe 450 455 460 Asp Trp Lys Leu Pro Asn Gly Gln SerHis Glu Asn Phe Asp Met Thr 465 470 475 480 Glu Ser Pro Gly Ile Ser AlaThr Arg Lys Asp Asp Leu Val Leu Ile 485 490 495 Ala Thr Pro Tyr Asp SerTyr 500 153 1538 DNA NICOTIANATABACUM 153 caactgcagt ttgaagataccaactaacca aaatgcagtt cttcagcttg gtttccattt 60 tcctatttct atcttttctctttttgttaa ggaaatggaa gaactcgaat agccaaagga 120 aaaaattgcc accaggtccatggaaactac caatactagg aagtatgctt catatggttg 180 gtggactacc acaccatgtccttagagatt tagccaaaaa atatggaccg cttatgcacc 240 ttcaattagg tgaagtttctgcagttgtgg ttacttctcc tgatatggca aaagaagtac 300 taaaaactca tgacatcgctttcgcgtcta ggcctagcct tttggccccg gagattgtct 360 gttacaatag gtctgatcttgcgttttgcc cctatggcga ttattggaga caaatgcgta 420 aaatatgtgt cttggaagtgctcagtgcca agaatgttcg gacatatagc tctattaggc 480 gcgatgaagt tcttcgtctccttaatttta tccggtcatc ttctggtgag cctgttaata 540 ttacggaaag gatctttttgttcacaagct ccatgacatg tagatcagcg tttgggcaag 600 tattcaagga gcaagacaaatttatacaac taattaaaga agttatactc ttagcaggag 660 ggtttgatgt ggctgacatattcccttcat acaagtctct tcatgtgctc agtggaatga 720 agggtaagat tatgaatgcacaccataagg tagatgctat tgttgagaat gtcatcaacg 780 agcacaagaa aaatcttgcaattgggaaaa ctaatggagc gttaggaggt gaagatttaa 840 ttgatgttct tctaaaacttatgaatgatg gaggccttca atttcctatc accaacgaca 900 acatcaaagc tataatctttgacatgtttg ctgctggaac agagacttca tcgtcaacaa 960 ttgtgtgggc tatggtggaaatggtgaaaa atccaactgt atttgcgaaa gctcaagcag 1020 aagtaagaga tgcatttagagaaaaagaaa cttttgatga aaatgatgtg gaggagctaa 1080 actatctaaa gttagtcattaaagaaactc taagacttca tccaccggtt ccacttttgc 1140 tcccaagaga atgtagggaagagacaaata taaacggcta cactattcct gtaaagacca 1200 aagtcatggt taatgtttgggcattgggaa gagatccaaa atattgggat gatgcagaaa 1260 cttttaagcc agagagatttgagcagtgct ctaaggattt tgttggtaat aattttgaat 1320 atcttccatt tggtggtggaaggaggattt gtccagggat ttcgtttggt ttagctaatg 1380 cttatttgcc attggctcaattactttatc actttgattg ggaactcccc actggaatca 1440 aaccaagcga cttggacttgactgagttgg ttggagtaac tgccgctaga aaaagtgacc 1500 tttacttggt tgcgactccttatcaacctc ctcaaaac 1538 154 502 PRT NICOTIANATABACUM 154 Met Gln PhePhe Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu 1 5 10 15 Phe LeuLeu Arg Lys Trp Lys Asn Ser Asn Ser Gln Arg Lys Lys Leu 20 25 30 Pro ProGly Pro Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met 35 40 45 Val GlyGly Leu Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr 50 55 60 Gly ProLeu Met His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val 65 70 75 80 ThrSer Pro Asp Met Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala 85 90 95 PheAla Ser Arg Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn 100 105 110Arg Ser Asp Leu Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met 115 120125 Arg Lys Ile Cys Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr 130135 140 Tyr Ser Ser Ile Arg Arg Asp Glu Val Leu Arg Leu Leu Asn Phe Ile145 150 155 160 Arg Ser Ser Ser Gly Glu Pro Val Asn Ile Thr Glu Arg IlePhe Leu 165 170 175 Phe Thr Ser Ser Met Thr Cys Arg Ser Ala Phe Gly GlnVal Phe Lys 180 185 190 Glu Gln Asp Lys Phe Ile Gln Leu Ile Lys Glu ValIle Leu Leu Ala 195 200 205 Gly Gly Phe Asp Val Ala Asp Ile Phe Pro SerTyr Lys Ser Leu His 210 215 220 Val Leu Ser Gly Met Lys Gly Lys Ile MetAsn Ala His His Lys Val 225 230 235 240 Asp Ala Ile Val Glu Asn Val IleAsn Glu His Lys Lys Asn Leu Ala 245 250 255 Ile Gly Lys Thr Asn Gly AlaLeu Gly Gly Glu Asp Leu Ile Asp Val 260 265 270 Leu Leu Lys Leu Met AsnAsp Gly Gly Leu Gln Phe Pro Ile Thr Asn 275 280 285 Asp Asn Ile Lys AlaIle Ile Phe Asp Met Phe Ala Ala Gly Thr Glu 290 295 300 Thr Ser Ser SerThr Ile Val Trp Ala Met Val Glu Met Val Lys Asn 305 310 315 320 Pro ThrVal Phe Ala Lys Ala Gln Ala Glu Val Arg Asp Ala Phe Arg 325 330 335 GluLys Glu Thr Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu 340 345 350Lys Leu Val Ile Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu 355 360365 Leu Leu Pro Arg Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr 370375 380 Ile Pro Val Lys Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg385 390 395 400 Asp Pro Lys Tyr Trp Asp Asp Ala Glu Thr Phe Lys Pro GluArg Phe 405 410 415 Glu Gln Cys Ser Lys Asp Phe Val Gly Asn Asn Phe GluTyr Leu Pro 420 425 430 Phe Gly Gly Gly Arg Arg Ile Cys Pro Gly Ile SerPhe Gly Leu Ala 435 440 445 Asn Ala Tyr Leu Pro Leu Ala Gln Leu Leu TyrHis Phe Asp Trp Glu 450 455 460 Leu Pro Thr Gly Ile Lys Pro Ser Asp LeuAsp Leu Thr Glu Leu Val 465 470 475 480 Gly Val Thr Ala Ala Arg Lys SerAsp Leu Tyr Leu Val Ala Thr Pro 485 490 495 Tyr Gln Pro Pro Gln Asn 500155 1530 DNA NICOTIANATABACUM 155 aaaagatgtc ttcattttcc acatcttctgccacttctaa ttccaaactt ccagttcgag 60 aaatcccagg agactatggt ttccccttttttggagccat aaaagataga tatgactact 120 tctacaacct cggcacagac gaattctttcttaccaaaat gcaaaaatac aactctactg 180 tctttagaac caacatgcca ccaggtccattcattgctaa aaatcccaaa gtaattgttc 240 tcctcgatgc caaaacattt cccgttcttttcgacaactc taaagtcgaa aaaatgaacg 300 ttcttgatgg cacgtacgtg ccatctactgatttctatgg cggatatcgc ccgtgtgctt 360 atcttgatcc ttctgagtca actcatgccacacttaaagg gttcttttta tctttaatct 420 cccagcttca taatcaattt attcctttatttagaacctc aatttctggt cttttcgcaa 480 atcttgagaa tgagatttcc caaaatggcaaagcgaactt caacaatatc agcgacatta 540 tgtcattcga ttttgttttt cgtttgttatgtgacaagac cagtccccat gacacaaatc 600 ttggctctaa tggaccaaaa ctctttgatatatggctgtt gcctcaactt gctccattgt 660 ttagtctagg tctaaaattt gtgccgaactttctggaaga tttaatgttg catacttttc 720 ccttgccatt ttttctagtg agatcgaattaccagaagct ttatgatgct tttagcaagc 780 atgccgaaag tacactgaat gaagcagagaagaatgggat caaaagagac gaagcatgcc 840 acaacttagt ttttcttgca ggtttcaatgcttatggtgg gatgaaagtt ttattccctg 900 cactgataaa gtgggtcgcc aatggaggaaagagtttaca cactcggctg gcaaatgaaa 960 tcaggacaat tatcaaagaa gaatgtgggaccataactct atcagcaatc aacaagatga 1020 gtttagtaaa atcagtagtg tatgaagtattaagaattga acctccagtt ccattccaat 1080 atggtaaagc caaagaagat atcataatccaaagccatga ttcaactttc ttagtcaaga 1140 aaggtgaaat gatctttgga tatcagccttttgctacaaa agatccaaag atttttgaca 1200 aaccagagga gtttattccg gagaggttcatggccgaagg ggaaaaatta ttaaagtatg 1260 tgtattggtc aaatgcaaga gagacagatgatccaacggt ggacaacaaa caatgcccag 1320 cgaaaaatct tgtcgtgctt ttgtgcaggttgatgttggt ggaggttttc atgcgttacg 1380 acacattcac agtggagtca acaaagctctttcttgggtc atcagtaacg ttcacgactc 1440 tggaaaaagc gacatgagtt tcagatatcttaattgtagg ctgcaaataa taatgtggtc 1500 attctgcaaa ttattgtact tgtgctgatg1530 156 483 PRT NICOTIANATABACUM 156 Met Ser Ser Phe Ser Thr Ser SerAla Thr Ser Asn Ser Lys Leu Pro 1 5 10 15 Val Arg Glu Ile Pro Gly AspTyr Gly Phe Pro Phe Phe Gly Ala Ile 20 25 30 Lys Asp Arg Tyr Asp Tyr PheTyr Asn Leu Gly Thr Asp Glu Phe Phe 35 40 45 Leu Thr Lys Met Gln Lys TyrAsn Ser Thr Val Phe Arg Thr Asn Met 50 55 60 Pro Pro Gly Pro Phe Ile AlaLys Asn Pro Lys Val Ile Val Leu Leu 65 70 75 80 Asp Ala Lys Thr Phe ProVal Leu Phe Asp Asn Ser Lys Val Glu Lys 85 90 95 Met Asn Val Leu Asp GlyThr Tyr Val Pro Ser Thr Asp Phe Tyr Gly 100 105 110 Gly Tyr Arg Pro CysAla Tyr Leu Asp Pro Ser Glu Ser Thr His Ala 115 120 125 Thr Leu Lys GlyPhe Phe Leu Ser Leu Ile Ser Gln Leu His Asn Gln 130 135 140 Phe Ile ProLeu Phe Arg Thr Ser Ile Ser Gly Leu Phe Ala Asn Leu 145 150 155 160 GluAsn Glu Ile Ser Gln Asn Gly Lys Ala Asn Phe Asn Asn Ile Ser 165 170 175Asp Ile Met Ser Phe Asp Phe Val Phe Arg Leu Leu Cys Asp Lys Thr 180 185190 Ser Pro His Asp Thr Asn Leu Gly Ser Asn Gly Pro Lys Leu Phe Asp 195200 205 Ile Trp Leu Leu Pro Gln Leu Ala Pro Leu Phe Ser Leu Gly Leu Lys210 215 220 Phe Val Pro Asn Phe Leu Glu Asp Leu Met Leu His Thr Phe ProLeu 225 230 235 240 Pro Phe Phe Leu Val Arg Ser Asn Tyr Gln Lys Leu TyrAsp Ala Phe 245 250 255 Ser Lys His Ala Glu Ser Thr Leu Asn Glu Ala GluLys Asn Gly Ile 260 265 270 Lys Arg Asp Glu Ala Cys His Asn Leu Val PheLeu Ala Gly Phe Asn 275 280 285 Ala Tyr Gly Gly Met Lys Val Leu Phe ProAla Leu Ile Lys Trp Val 290 295 300 Ala Asn Gly Gly Lys Ser Leu His ThrArg Leu Ala Asn Glu Ile Arg 305 310 315 320 Thr Ile Ile Lys Glu Glu CysGly Thr Ile Thr Leu Ser Ala Ile Asn 325 330 335 Lys Met Ser Leu Val LysSer Val Val Tyr Glu Val Leu Arg Ile Glu 340 345 350 Pro Pro Val Pro PheGln Tyr Gly Lys Ala Lys Glu Asp Ile Ile Ile 355 360 365 Gln Ser His AspSer Thr Phe Leu Val Lys Lys Gly Glu Met Ile Phe 370 375 380 Gly Tyr GlnPro Phe Ala Thr Lys Asp Pro Lys Ile Phe Asp Lys Pro 385 390 395 400 GluGlu Phe Ile Pro Glu Arg Phe Met Ala Glu Gly Glu Lys Leu Leu 405 410 415Lys Tyr Val Tyr Trp Ser Asn Ala Arg Glu Thr Asp Asp Pro Thr Val 420 425430 Asp Asn Lys Gln Cys Pro Ala Lys Asn Leu Val Val Leu Leu Cys Arg 435440 445 Leu Met Leu Val Glu Val Phe Met Arg Tyr Asp Thr Phe Thr Val Glu450 455 460 Ser Thr Lys Leu Phe Leu Gly Ser Ser Val Thr Phe Thr Thr LeuGlu 465 470 475 480 Lys Ala Thr 157 1593 DNA NICOTIANATABACUM 157ccaaaaatgg agcttcaatc ttctcctttc aatttaattt ctttgttcct cttcttttct 60tttcatttta ttctagtgaa gaaatggaat gccaaaatcc caaagttacc tccaggtccg 120tggaggcttc cctttattgg aagcctccat cacttgaagg gaaaacttcc acaccataat 180cttagagatc tagcgcgaaa atatgggcct ctcatgtact tacaactcgg agaaattcct 240gtagttgtaa tatcttcgcc acgtgtagca aaagctgtac taaaaactca tgatctcgct 300tttgcaacta gaccacgatt catgtcctca gacattgtgt tttacaaaag cagggacatc 360tcttttgccc catttggtga ttactggaga cagatgcgta aaatattgac tcaggaactc 420ctgagtaaca agatgctcaa gtcatatagc ttaatccgaa aggatgagct ctcgaagctc 480ctctcatcga ttcgtttgga aacaggttct gcagtgaaca taaatgaaaa gcttctctgg 540tttacgagct gcatgacctg tagattagcc tttggaaaaa tatgcaatga tcgggatgag 600ttgatcatgc taattaggga gatattaaca ttatcaggag gatttgatgt gggtgatttg 660ttcccttcct ggaaattact tcataatatg agcaacatga aagctaggtt gacgaatgta 720caccacaagt atgatttagt tatggagaac atcatcaatg agcaccaaga gaatcatgca 780gcagggataa agggtaacaa cgagtttggt ggcgaagata tgatcgatgc tctactgagg 840gctaaggaga ataatgagct tcaatttcct atcgaaaatg acaacatgaa agcagtaatt 900ctggacttgt ttattgctgg aactgaaact tcatatactg caattatatg ggcactatca 960gaattgatga agcacccaag tgtgatggcc aaggcacaag ctgaagtgag aaaagtcttc 1020aaagaaaatg aaaatttcga cgaaaatgat cttgacaagt tgccatactt aaaatcagtg 1080attaaagaaa cactaaggat gcaccctcca gttcctttgt tagggcctag agaatgcagg 1140gaccaaacag agatcgatgg ctacactgta cctattaaag ctagagttat ggttaatgct 1200tgggcgatag gaagagatcc tgaaagttgg gaagatcctg aaagtttcaa accggagcga 1260tttgaaaata cttctgttga tcttacagga aatcactatc agttcattcc tttcggttca 1320ggaagaagaa tgtgtccagg aatgtcgttt ggtttagtta acacagggca tcctttagcc 1380cagttgctct attgctttga ctggaaactc cctgacaagg ttaatgcaaa tgattttcgc 1440actactgaaa caagtagagt ttttgcagca agcaaagatg acctctactt gattcccaca 1500aatcacaggg agcaagaata gcttaattta atggagttct tggaagaatt aaagaagaag 1560ggctatatag gtgagatttt ttgtatggtt gca 1593 158 504 PRT NICOTIANATABACUM158 Met Glu Leu Gln Ser Ser Pro Phe Asn Leu Ile Ser Leu Phe Leu Phe 1 510 15 Phe Ser Phe His Phe Ile Leu Val Lys Lys Trp Asn Ala Lys Ile Pro 2025 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Phe Ile Gly Ser Leu His 3540 45 His Leu Lys Gly Lys Leu Pro His His Asn Leu Arg Asp Leu Ala Arg 5055 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Ile Pro Val Val 6570 75 80 Val Ile Ser Ser Pro Arg Val Ala Lys Ala Val Leu Lys Thr His Asp85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Phe Gly Asp Tyr TrpArg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn LysMet Leu 130 135 140 Lys Ser Tyr Ser Leu Ile Arg Lys Asp Glu Leu Ser LysLeu Leu Ser 145 150 155 160 Ser Ile Arg Leu Glu Thr Gly Ser Ala Val AsnIle Asn Glu Lys Leu 165 170 175 Leu Trp Phe Thr Ser Cys Met Thr Cys ArgLeu Ala Phe Gly Lys Ile 180 185 190 Cys Asn Asp Arg Asp Glu Leu Ile MetLeu Ile Arg Glu Ile Leu Thr 195 200 205 Leu Ser Gly Gly Phe Asp Val GlyAsp Leu Phe Pro Ser Trp Lys Leu 210 215 220 Leu His Asn Met Ser Asn MetLys Ala Arg Leu Thr Asn Val His His 225 230 235 240 Lys Tyr Asp Leu ValMet Glu Asn Ile Ile Asn Glu His Gln Glu Asn 245 250 255 His Ala Ala GlyIle Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp Met 260 265 270 Ile Asp AlaLeu Leu Arg Ala Lys Glu Asn Asn Glu Leu Gln Phe Pro 275 280 285 Ile GluAsn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile Ala 290 295 300 GlyThr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu Leu 305 310 315320 Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg Lys 325330 335 Val Phe Lys Glu Asn Glu Asn Phe Asp Glu Asn Asp Leu Asp Lys Leu340 345 350 Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His ProPro 355 360 365 Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Asp Gln Thr GluIle Asp 370 375 380 Gly Tyr Thr Val Pro Ile Lys Ala Arg Val Met Val AsnAla Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp ProGlu Ser Phe Lys Pro 405 410 415 Glu Arg Phe Glu Asn Thr Ser Val Asp LeuThr Gly Asn His Tyr Gln 420 425 430 Phe Ile Pro Phe Gly Ser Gly Arg ArgMet Cys Pro Gly Met Ser Phe 435 440 445 Gly Leu Val Asn Thr Gly His ProLeu Ala Gln Leu Leu Tyr Cys Phe 450 455 460 Asp Trp Lys Leu Pro Asp LysVal Asn Ala Asn Asp Phe Arg Thr Thr 465 470 475 480 Glu Thr Ser Arg ValPhe Ala Ala Ser Lys Asp Asp Leu Tyr Leu Ile 485 490 495 Pro Thr Asn HisArg Glu Gln Glu 500 159 1599 DNA NICOTIANATABACUM 159 cttcttccaaaaatggagct tcaatcttct cctttcaatt taatttcttt gttcctcttc 60 ttttcttttctttttattct agtgaagaaa tggaatgcca aaatcccaaa gttacctcca 120 ggtccgtggaggcttccctt tattggaagc ctccatcact tgaagggaaa acttccacac 180 cataatcttagagatctagc gcgaaaatat ggacctctca tgtacttaca actcggagaa 240 attcctgtagttgtaatatc ttcgccacgt gtagcaaaag ctgtactaaa aactcatgat 300 ctcgcttttgcaactagacc acgattcatg tcctcagaca ttgtgtttta caaaagcagg 360 gacatctcttttgccccatt tggtgattac tggagacaga tgcgtaaaat attgactcag 420 gaactcctgagtaacaagat gctcaagtca tatagcttaa tccgaaagga tgagctctcg 480 aagctcctctcatcgattcg tttggaaaca ggttctgcag tgaacataaa tgaaaagctt 540 ctctggtttacgagctgcat gacctgtaga ttagcctttg gaaaaatatg caatgatcgg 600 gatgagttgatcatgctaat tagggagata ttaacattat caggaggatt tgatgtgggt 660 gatttgttcccttcctggaa attacttcat aatatgagca acatgaaagc taggttgacg 720 aatgtacaccacaagtatga tttagttatg gagaacatca tcaatgagca ccaagagaat 780 catgcagcagggataaaggg taacaacgag tttggtggcg aagatatgat cgatgctcta 840 ctgagggctaaggagaataa tgagcttcaa tttcctatcg aaaatgacaa catgaaagca 900 gtaattctggacttgtttat tgctggaact gaaacttcat atactgcaat tatatgggca 960 ctatcagaattgatgaagca cccaagtgtg atggccaagg cacaagctga agtgagaaaa 1020 gtcttcaaagaaaatgaaaa tttcgacgaa aatgatcttg acaagttgcc atacttaaaa 1080 tcagtgattaaagaaacact aaggatgcac cctccagttc ctttgttagg gcctagagaa 1140 tgcagggaccaaacagagat cgatggctac actgtaccta ttaaagctag agttatggtt 1200 aatgcttgggcgataggaag agatcctgaa agttgggaag atcctgaaag tttcaaaccg 1260 gagcgatttgaaaatacttc tgttgatctt acaggaaatc actatcagtt cattcctttc 1320 ggttcaggaagaagaatgtg tccaggaatg tcgtttggtt tagttaacac agggcatcct 1380 ttagcccagttgctctattg ctttgactgg aaactccctg acaaggttaa tgcaaatgat 1440 tttcgcactactgaaacaag tagagttttt gcagcaagca aagatgacct ctacttgatt 1500 cccacaaatcacagggagca agaatagctt aatttaatgg agttcttgga agaattaaag 1560 aagaagggctatataggtga gattttttgt atggttgca 1599 160 504 PRT NICOTIANATABACUM 160Met Glu Leu Gln Ser Ser Pro Phe Asn Leu Ile Ser Leu Phe Leu Phe 1 5 1015 Phe Ser Phe Leu Phe Ile Leu Val Lys Lys Trp Asn Ala Lys Ile Pro 20 2530 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Phe Ile Gly Ser Leu His 35 4045 His Leu Lys Gly Lys Leu Pro His His Asn Leu Arg Asp Leu Ala Arg 50 5560 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Ile Pro Val Val 65 7075 80 Val Ile Ser Ser Pro Arg Val Ala Lys Ala Val Leu Lys Thr His Asp 8590 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Phe Gly Asp Tyr TrpArg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn LysMet Leu 130 135 140 Lys Ser Tyr Ser Leu Ile Arg Lys Asp Glu Leu Ser LysLeu Leu Ser 145 150 155 160 Ser Ile Arg Leu Glu Thr Gly Ser Ala Val AsnIle Asn Glu Lys Leu 165 170 175 Leu Trp Phe Thr Ser Cys Met Thr Cys ArgLeu Ala Phe Gly Lys Ile 180 185 190 Cys Asn Asp Arg Asp Glu Leu Ile MetLeu Ile Arg Glu Ile Leu Thr 195 200 205 Leu Ser Gly Gly Phe Asp Val GlyAsp Leu Phe Pro Ser Trp Lys Leu 210 215 220 Leu His Asn Met Ser Asn MetLys Ala Arg Leu Thr Asn Val His His 225 230 235 240 Lys Tyr Asp Leu ValMet Glu Asn Ile Ile Asn Glu His Gln Glu Asn 245 250 255 His Ala Ala GlyIle Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp Met 260 265 270 Ile Asp AlaLeu Leu Arg Ala Lys Glu Asn Asn Glu Leu Gln Phe Pro 275 280 285 Ile GluAsn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile Ala 290 295 300 GlyThr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu Leu 305 310 315320 Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg Lys 325330 335 Val Phe Lys Glu Asn Glu Asn Phe Asp Glu Asn Asp Leu Asp Lys Leu340 345 350 Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His ProPro 355 360 365 Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Asp Gln Thr GluIle Asp 370 375 380 Gly Tyr Thr Val Pro Ile Lys Ala Arg Val Met Val AsnAla Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp ProGlu Ser Phe Lys Pro 405 410 415 Glu Arg Phe Glu Asn Thr Ser Val Asp LeuThr Gly Asn His Tyr Gln 420 425 430 Phe Ile Pro Phe Gly Ser Gly Arg ArgMet Cys Pro Gly Met Ser Phe 435 440 445 Gly Leu Val Asn Thr Gly His ProLeu Ala Gln Leu Leu Tyr Cys Phe 450 455 460 Asp Trp Lys Leu Pro Asp LysVal Asn Ala Asn Asp Phe Arg Thr Thr 465 470 475 480 Glu Thr Ser Arg ValPhe Ala Ala Ser Lys Asp Asp Leu Tyr Leu Ile 485 490 495 Pro Thr Asn HisArg Glu Gln Glu 500 161 1564 DNA NICOTIANATABACUM 161 ctttctttcttgtaccgaga tggagtttca acacttggtt tcgttcttgc tattcatctc 60 cttcatctttcttctaattc aaaaatggag gaaatcgaaa aagctgccac ctggtccgtg 120 gaggctacctattattggaa gtgtgcatca cttgacaagt ggagtaccac atcgagttct 180 cagaaatttatcacaaaaat ttggcccgat catgtacttg cagctcgggg aagttcccac 240 agtagttgtatcctccccac acatggccaa acaaatttta aaaactcatg acctcgcttt 300 tgcatctaggccagaaatca tgatgggaaa aattatttgc tacgattgta aggacattgc 360 cttttccccgtatggtgatt attggagaca tatgcgtaaa ttgagcacct tggaactact 420 tagtgccaagatggtcaagt ccttcagtcc aattcgtcaa gatgagctct caagtctcct 480 atcatccattgaatcaatgg gaaatttgcc aatcaactta gtagaaaaac ttttatggtt 540 tatgaatgccgcgacatgta ggtcagcatt tgggaaagtg tgtaaagatc aaaaagagtt 600 gataacattgattcaacgag cagaatcatt atctggtgga ttcgagctgg ctgatttgtt 660 cccttcgaagaagtttctac atggtattag tgggatgcga tctaaactaa tggaagctcg 720 taacaagatagacgcagtct tggacaacat tatcaatgtg cacagagaga atcgggcaaa 780 tggaaatagttgtaatggtg agtctggaac tgtagatttc atcgatgttt ttctaagggt 840 catggagagtggcgaattac catttccgat agaaaatgac aacatcaaag cagttattct 900 tgacatgttcgtagcaggat ctgacacatc atcttcaacc gttatttggg cattaacaga 960 aatgatgaagaatccaaaag tcatggctaa agcacaagct gaagtgagag aagcttttaa 1020 aggaaagaaagcatgtgatg aggatactga tcttgaaaag cttcattacc taaatttagt 1080 gatcaaagagacactccgat tacaccctcc aactcctcta cttgtcccgc gagaatgcag 1140 ggaggaaacagagatagaag gattcactat accattgaaa agcaaagtct tggttaacgt 1200 atgggcaattggaagagatc ccgagaattg gaaaaatcct gaatgtttta taccagagag 1260 attcgaaaatagttctattg agtttactgg aaatcatttt caacttcttc cgtttggcgc 1320 tggaagacgaatttgtccag gaatgcaatt tggtttggct cttgttactc tgccattggc 1380 tcatttgcttcacaattttg attggaaact tcccgaagga attaatgcaa gggatttgga 1440 catgacagaggcaaatggga tatctgctag aagagaaaaa gatctttact tgattgctac 1500 tccttatgtatcacctcttg attaactctg aaattttgct ttaatgctgc ttgcttgctt 1560 cact 1564162 501 PRT NICOTIANATABACUM 162 Met Glu Phe Gln His Leu Val Ser Phe LeuLeu Phe Ile Ser Phe Ile 1 5 10 15 Phe Leu Leu Ile Gln Lys Trp Arg LysSer Lys Lys Leu Pro Pro Gly 20 25 30 Pro Trp Arg Leu Pro Ile Ile Gly SerVal His His Leu Thr Ser Gly 35 40 45 Val Pro His Arg Val Leu Arg Asn LeuSer Gln Lys Phe Gly Pro Ile 50 55 60 Met Tyr Leu Gln Leu Gly Glu Val ProThr Val Val Val Ser Ser Pro 65 70 75 80 His Met Ala Lys Gln Ile Leu LysThr His Asp Leu Ala Phe Ala Ser 85 90 95 Arg Pro Glu Ile Met Met Gly LysIle Ile Cys Tyr Asp Cys Lys Asp 100 105 110 Ile Ala Phe Ser Pro Tyr GlyAsp Tyr Trp Arg His Met Arg Lys Leu 115 120 125 Ser Thr Leu Glu Leu LeuSer Ala Lys Met Val Lys Ser Phe Ser Pro 130 135 140 Ile Arg Gln Asp GluLeu Ser Ser Leu Leu Ser Ser Ile Glu Ser Met 145 150 155 160 Gly Asn LeuPro Ile Asn Leu Val Glu Lys Leu Leu Trp Phe Met Asn 165 170 175 Ala AlaThr Cys Arg Ser Ala Phe Gly Lys Val Cys Lys Asp Gln Lys 180 185 190 GluLeu Ile Thr Leu Ile Gln Arg Ala Glu Ser Leu Ser Gly Gly Phe 195 200 205Glu Leu Ala Asp Leu Phe Pro Ser Lys Lys Phe Leu His Gly Ile Ser 210 215220 Gly Met Arg Ser Lys Leu Met Glu Ala Arg Asn Lys Ile Asp Ala Val 225230 235 240 Leu Asp Asn Ile Ile Asn Val His Arg Glu Asn Arg Ala Asn GlyAsn 245 250 255 Ser Cys Asn Gly Glu Ser Gly Thr Val Asp Phe Ile Asp ValPhe Leu 260 265 270 Arg Val Met Glu Ser Gly Glu Leu Pro Phe Pro Ile GluAsn Asp Asn 275 280 285 Ile Lys Ala Val Ile Leu Asp Met Phe Val Ala GlySer Asp Thr Ser 290 295 300 Ser Ser Thr Val Ile Trp Ala Leu Thr Glu MetMet Lys Asn Pro Lys 305 310 315 320 Val Met Ala Lys Ala Gln Ala Glu ValArg Glu Ala Phe Lys Gly Lys 325 330 335 Lys Ala Cys Asp Glu Asp Thr AspLeu Glu Lys Leu His Tyr Leu Asn 340 345 350 Leu Val Ile Lys Glu Thr LeuArg Leu His Pro Pro Thr Pro Leu Leu 355 360 365 Val Pro Arg Glu Cys ArgGlu Glu Thr Glu Ile Glu Gly Phe Thr Ile 370 375 380 Pro Leu Lys Ser LysVal Leu Val Asn Val Trp Ala Ile Gly Arg Asp 385 390 395 400 Pro Glu AsnTrp Lys Asn Pro Glu Cys Phe Ile Pro Glu Arg Phe Glu 405 410 415 Asn SerSer Ile Glu Phe Thr Gly Asn His Phe Gln Leu Leu Pro Phe 420 425 430 GlyAla Gly Arg Arg Ile Cys Pro Gly Met Gln Phe Gly Leu Ala Leu 435 440 445Val Thr Leu Pro Leu Ala His Leu Leu His Asn Phe Asp Trp Lys Leu 450 455460 Pro Glu Gly Ile Asn Ala Arg Asp Leu Asp Met Thr Glu Ala Asn Gly 465470 475 480 Ile Ser Ala Arg Arg Glu Lys Asp Leu Tyr Leu Ile Ala Thr ProTyr 485 490 495 Val Ser Pro Leu Asp 500 163 1560 DNA NICOTIANATABACUM163 ctttcttgta ccgagatgga gtttcaacac ttggtttcgt tcttgctatt catctccttc 60atctttcttc taattcaaaa atggaggaaa tcgaaaaagc tgccacctgg tccgtggagg 120ctacctatta ttggaagtgt gcatcacttg acaagtggag taccacatcg agttctcaga 180aatttatcac aaaaatttgg cccgatcatg tacttgcagc tcggggaagt tcccacagta 240gttgtatcct ccccacacat ggccaaacaa attttaaaaa ctcatgacct cgcttttgca 300tctaggccag aaatcatgat gggaaaaatt atttgctacg attgtaagga cattgccttt 360tccccgtatg gtgattattg gagacatatg cgtaaattga gcaccttgga actacttagt 420gccaagatgg tcaagtcctt cagtccaatt cgtcaagatg agctctcaag tctcctatca 480tccattgaat caatgggaaa tttgccaatc aacttagtag aaaaactttt atggtttatg 540aatgccgcga catgtaggtc agcatttggg aaagtgtgta aagatcaaaa agagttgata 600acattgattc aacgagcaga atcattatct ggtggattcg agctggctga tttgttccct 660tcgaagaagt ttctacatgg tattagtggg atgcgatcta aactaatgga agctcgtaac 720aagatagacg cagtcttgga caacattatc aatgtgcaca gagagaatcg ggcaaatgga 780aatagttgta atggtgagtc tggaactgta gatttcatcg atgtttttct aagggtcatg 840gagagtggcg aattaccatt tccgatagaa aatgacaaca tcaaagcagt tattcttgac 900atgttcgtag caggatctga cacatcatct tcaaccgtta tttgggcatt aacagaaacg 960atgaagaatc caaaagtcat ggctaaagca caagctgaag tgagagaagc ttttaaagga 1020aagaaagcat gtgatgagga tactgatctt gaaaagcatc attacctaaa tttagtgatc 1080aaagagacac tccgattaca ccctccaact cctctacttg tcccgcgaga atgcagggag 1140gaaacagaga tagaaggatt cactatacca ttgaaaagca aagtcttggt taacgtatgg 1200gcaattggaa gagatcccga gaattggaaa aatcctgaat gttttatacc agagagattc 1260gaaaatagtt ctattgagtt tactggaaat cattttcaac ttcttccgtt tggcgctgga 1320agacgaattt gtccaggaat gcaatttggt ttggctcttg ttactctgcc attggctcat 1380ttgcttcaca attttgattg gaaacttccc gaaggaatta atgcaaggga tttggacatg 1440acagaggcaa atgggatatc tgctagaaga gaaaaagatc tttacttgat tgctactcct 1500tatgtatcac ctcttgatta actctgaaat tttgctttaa tgctgcttgc ttgcttcact 1560164 501 PRT NICOTIANATABACUM 164 Met Glu Phe Gln His Leu Val Ser Phe LeuLeu Phe Ile Ser Phe Ile 1 5 10 15 Phe Leu Leu Ile Gln Lys Trp Arg LysSer Lys Lys Leu Pro Pro Gly 20 25 30 Pro Trp Arg Leu Pro Ile Ile Gly SerVal His His Leu Thr Ser Gly 35 40 45 Val Pro His Arg Val Leu Arg Asn LeuSer Gln Lys Phe Gly Pro Ile 50 55 60 Met Tyr Leu Gln Leu Gly Glu Val ProThr Val Val Val Ser Ser Pro 65 70 75 80 His Met Ala Lys Gln Ile Leu LysThr His Asp Leu Ala Phe Ala Ser 85 90 95 Arg Pro Glu Ile Met Met Gly LysIle Ile Cys Tyr Asp Cys Lys Asp 100 105 110 Ile Ala Phe Ser Pro Tyr GlyAsp Tyr Trp Arg His Met Arg Lys Leu 115 120 125 Ser Thr Leu Glu Leu LeuSer Ala Lys Met Val Lys Ser Phe Ser Pro 130 135 140 Ile Arg Gln Asp GluLeu Ser Ser Leu Leu Ser Ser Ile Glu Ser Met 145 150 155 160 Gly Asn LeuPro Ile Asn Leu Val Glu Lys Leu Leu Trp Phe Met Asn 165 170 175 Ala AlaThr Cys Arg Ser Ala Phe Gly Lys Val Cys Lys Asp Gln Lys 180 185 190 GluLeu Ile Thr Leu Ile Gln Arg Ala Glu Ser Leu Ser Gly Gly Phe 195 200 205Glu Leu Ala Asp Leu Phe Pro Ser Lys Lys Phe Leu His Gly Ile Ser 210 215220 Gly Met Arg Ser Lys Leu Met Glu Ala Arg Asn Lys Ile Asp Ala Val 225230 235 240 Leu Asp Asn Ile Ile Asn Val His Arg Glu Asn Arg Ala Asn GlyAsn 245 250 255 Ser Cys Asn Gly Glu Ser Gly Thr Val Asp Phe Ile Asp ValPhe Leu 260 265 270 Arg Val Met Glu Ser Gly Glu Leu Pro Phe Pro Ile GluAsn Asp Asn 275 280 285 Ile Lys Ala Val Ile Leu Asp Met Phe Val Ala GlySer Asp Thr Ser 290 295 300 Ser Ser Thr Val Ile Trp Ala Leu Thr Glu ThrMet Lys Asn Pro Lys 305 310 315 320 Val Met Ala Lys Ala Gln Ala Glu ValArg Glu Ala Phe Lys Gly Lys 325 330 335 Lys Ala Cys Asp Glu Asp Thr AspLeu Glu Lys His His Tyr Leu Asn 340 345 350 Leu Val Ile Lys Glu Thr LeuArg Leu His Pro Pro Thr Pro Leu Leu 355 360 365 Val Pro Arg Glu Cys ArgGlu Glu Thr Glu Ile Glu Gly Phe Thr Ile 370 375 380 Pro Leu Lys Ser LysVal Leu Val Asn Val Trp Ala Ile Gly Arg Asp 385 390 395 400 Pro Glu AsnTrp Lys Asn Pro Glu Cys Phe Ile Pro Glu Arg Phe Glu 405 410 415 Asn SerSer Ile Glu Phe Thr Gly Asn His Phe Gln Leu Leu Pro Phe 420 425 430 GlyAla Gly Arg Arg Ile Cys Pro Gly Met Gln Phe Gly Leu Ala Leu 435 440 445Val Thr Leu Pro Leu Ala His Leu Leu His Asn Phe Asp Trp Lys Leu 450 455460 Pro Glu Gly Ile Asn Ala Arg Asp Leu Asp Met Thr Glu Ala Asn Gly 465470 475 480 Ile Ser Ala Arg Arg Glu Lys Asp Leu Tyr Leu Ile Ala Thr ProTyr 485 490 495 Val Ser Pro Leu Asp 500 165 1552 DNA NICOTIANATABACUM165 caaaaacaaa attccaatgg ttaacatgtt cactccaatt atatacgctc ctctcctttt 60agctttttac attatcacaa aacatttctt acgcaaactc agaaataatc caccagctcc 120atttcttact ttccccttta ttggccatct ttatctcttc aaaaaaccac ttcaacgtac 180cttagccaaa atctccgaac gttatggctc tgttcttcta ctcgaattcg gttcacgaaa 240agtacttttg gtttcttcac catctgcagc tgaagaatgc ttaacaaaaa acgatattat 300tttcgcgaat cgtcctcttt tgatggctgg aaaacatctt ggatataatt ttacatcttt 360ggcttggagt tcgtacggag atcattggag aaatctgcga aggattactt cagttgagat 420gttttcgact catcgtcttc aaatgctaca tgggattcgt attgatgaag tgaaatctat 480ggttaagagg ctcaattcct ctgccatagc tgaaaaatct gtggatatga agtctatgtt 540ttttgagctg atgctcaatg ttatgatgag gacaattgct ggaaaaagat attacggtga 600gaatgtggag gacattgagg aagctacgag attcaaaggt ttggtgcaag agactttcag 660gattggcggg gcgacgaata ttggcgactt tttgccggcg ttgaagttat tggtgaggaa 720attggagaaa agtttaattg tgttgcaaga gaacagagat gagtttatgc aggaattaat 780taaagattgc agaaaaagaa tggagaaaga aggtactgtt actgattcag aaattgaagg 840gaacaagaaa tgtttaattg aagttttgtt aacactacaa gaaaatgaac cggaatacta 900caaagatgaa atcatcagaa gccttatgct tgttctatta tcagctggta cagatacttc 960agttgggaca atggaatggg ctttatcatt aatgttaaac caccctgaaa ctctgaagaa 1020agcacaagct gaaattgatg aacatatagg acatgaacgt ttagtggacg agtcggacat 1080caacaaccta ccttacctac gttgtataat caacgagaca ttccgaatgt accctgcagg 1140accactacta gtcccacacg agtcgtcaga ggaaaccacc gtaggaggct accgtgtacc 1200cggaggaacc atgttacttg tgaatttgtg ggcaattcac aatgatccaa agctatggga 1260tgaaccaaga aagtttaaac cagaaagatt tcaaggacta gatggtgtta gagatggtta 1320caaaatgatg ccttttggtt ctggacgaag gagttgtcct ggagaaggat tggctgttcg 1380aatggttgcc ttgtcattgg gatgtattat tcaatgtttt gattggcaac gaatcggcga 1440agaattggtt gatatgactg aaggaactgg acttactttg cctaaagctc aacctttggt 1500ggccaagtgt agcccacgac ctaaaatggc taatcttctc tctcagattt ga 1552 166 511PRT NICOTIANATABACUM 166 Met Val Asn Met Phe Thr Pro Ile Ile Tyr Ala ProLeu Leu Leu Ala 1 5 10 15 Phe Tyr Ile Ile Thr Lys His Phe Leu Arg LysLeu Arg Asn Asn Pro 20 25 30 Pro Ala Pro Phe Leu Thr Phe Pro Phe Ile GlyHis Leu Tyr Leu Phe 35 40 45 Lys Lys Pro Leu Gln Arg Thr Leu Ala Lys IleSer Glu Arg Tyr Gly 50 55 60 Ser Val Leu Leu Leu Glu Phe Gly Ser Arg LysVal Leu Leu Val Ser 65 70 75 80 Ser Pro Ser Ala Ala Glu Glu Cys Leu ThrLys Asn Asp Ile Ile Phe 85 90 95 Ala Asn Arg Pro Leu Leu Met Ala Gly LysHis Leu Gly Tyr Asn Phe 100 105 110 Thr Ser Leu Ala Trp Ser Ser Tyr GlyAsp His Trp Arg Asn Leu Arg 115 120 125 Arg Ile Thr Ser Val Glu Met PheSer Thr His Arg Leu Gln Met Leu 130 135 140 His Gly Ile Arg Ile Asp GluVal Lys Ser Met Val Lys Arg Leu Asn 145 150 155 160 Ser Ser Ala Ile AlaGlu Lys Ser Val Asp Met Lys Ser Met Phe Phe 165 170 175 Glu Leu Met LeuAsn Val Met Met Arg Thr Ile Ala Gly Lys Arg Tyr 180 185 190 Tyr Gly GluAsn Val Glu Asp Ile Glu Glu Ala Thr Arg Phe Lys Gly 195 200 205 Leu ValGln Glu Thr Phe Arg Ile Gly Gly Ala Thr Asn Ile Gly Asp 210 215 220 PheLeu Pro Ala Leu Lys Leu Leu Val Arg Lys Leu Glu Lys Ser Leu 225 230 235240 Ile Val Leu Gln Glu Asn Arg Asp Glu Phe Met Gln Glu Leu Ile Lys 245250 255 Asp Cys Arg Lys Arg Met Glu Lys Glu Gly Thr Val Thr Asp Ser Glu260 265 270 Ile Glu Gly Asn Lys Lys Cys Leu Ile Glu Val Leu Leu Thr LeuGln 275 280 285 Glu Asn Glu Pro Glu Tyr Tyr Lys Asp Glu Ile Ile Arg SerLeu Met 290 295 300 Leu Val Leu Leu Ser Ala Gly Thr Asp Thr Ser Val GlyThr Met Glu 305 310 315 320 Trp Ala Leu Ser Leu Met Leu Asn His Pro GluThr Leu Lys Lys Ala 325 330 335 Gln Ala Glu Ile Asp Glu His Ile Gly HisGlu Arg Leu Val Asp Glu 340 345 350 Ser Asp Ile Asn Asn Leu Pro Tyr LeuArg Cys Ile Ile Asn Glu Thr 355 360 365 Phe Arg Met Tyr Pro Ala Gly ProLeu Leu Val Pro His Glu Ser Ser 370 375 380 Glu Glu Thr Thr Val Gly GlyTyr Arg Val Pro Gly Gly Thr Met Leu 385 390 395 400 Leu Val Asn Leu TrpAla Ile His Asn Asp Pro Lys Leu Trp Asp Glu 405 410 415 Pro Arg Lys PheLys Pro Glu Arg Phe Gln Gly Leu Asp Gly Val Arg 420 425 430 Asp Gly TyrLys Met Met Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro 435 440 445 Gly GluGly Leu Ala Val Arg Met Val Ala Leu Ser Leu Gly Cys Ile 450 455 460 IleGln Cys Phe Asp Trp Gln Arg Ile Gly Glu Glu Leu Val Asp Met 465 470 475480 Thr Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln Pro Leu Val Ala 485490 495 Lys Cys Ser Pro Arg Pro Lys Met Ala Asn Leu Leu Ser Gln Ile 500505 510 167 1675 DNA NICOTIANATABACUM 167 caaaaacaaa attccaatggttaacatgtt cactccaatt atatacgctc ctctcctttt 60 agctttttac attatcacaaaacatttctt acgcaaactc agaaataacc caccagctcc 120 atttcttact ttcccctttattggccatct ttatctcttc aaaaaaccac ttcaacgtac 180 cttagccaaa atctccgaacgttatggctc tgttcttcta ctcgaattcg gttcacgaaa 240 agtacttttg gtttcttcaccatctgcagc tgaagaatgc ttaacaaaaa acgatattat 300 tttcgcgaat cgtcctcttttgatggctgg aaaacatctt ggatataatt ttacttcttt 360 ggcttggagt tcgtacggagatcactggag aaatcttcgt aggattactt cagttgagat 420 gttttcgact catcgtcttcaaatgctaca tggaattcgt attgatgaag tgaaatctat 480 ggttaagagg ctcaattcctctgccatagc tgaaaaatct gtggatatga agtctatgtt 540 ttttgagctg atgctcaatgttatgatgag gacaattgct ggaaaaagat attacggtga 600 gaatgtggag gacattgaggaagctacgag attcaaaggt ttggtgcaag agactttcag 660 gattggcggg gcgacgaatattggcgactt tttgccggcg ttgaagttat tggtgaggaa 720 attggagaaa agtttaattgtgttgcaaga gaacagagat gagtttatgc aggaattaat 780 taaagattgc agaaaaagaatggagaaaga aggtactgtt actgattcag aaattgaagg 840 gaacaagaaa tgtttaattgaagttttgtt aacactacaa gaaaatgaac cggaatacta 900 caaagatgaa atcatcagaagccttatgct tgttctatta tcagctggta cagatacttc 960 agttgggaca atggaatgggctttatcatt aatgttaaac caccctgaaa ctctgaagaa 1020 agcacaagct gaaattgatgaacatatagg acatgaacgt ttagtggacg agtcggacat 1080 caacaaccta ccttacctacgttgtataat caacgagaca ttccgaatgt accctgcagg 1140 accactacta gtcccacacgagtcgtcaga ggaaaccacc gtaggaggct accgtgtacc 1200 cggaggaacc atgttacttgtgaatttgtg ggctattcac aatgatccaa agctatggga 1260 tgaaccaaga aagtttaagccagaaagatt tgaaggacta gaaggtgtta gagacggtta 1320 caaaatgatg ccttttggttctggacgaag gagttgtcct ggagaaggat tggctattcg 1380 aatggttgca ttgtcattgggatgtattat tcaatgcttt gattggcaac gacttgggga 1440 aggattggtt gataagactgaaggaactgg acttactttg cctaaagctc aacctttagt 1500 ggccaagtgt agcccacgacctataatggc taatcttctt tctcagattt gaacataatt 1560 ggtttctacc aaacatccccaaactagaat attattattg gttacatata caatgtaatc 1620 aattttgaac catattatatctcaatgtat tcctttttaa aaaaaaaaaa aaaaa 1675 168 511 PRT NICOTIANATABACUM168 Met Val Asn Met Phe Thr Pro Ile Ile Tyr Ala Pro Leu Leu Leu Ala 1 510 15 Phe Tyr Ile Ile Thr Lys His Phe Leu Arg Lys Leu Arg Asn Asn Pro 2025 30 Pro Ala Pro Phe Leu Thr Phe Pro Phe Ile Gly His Leu Tyr Leu Phe 3540 45 Lys Lys Pro Leu Gln Arg Thr Leu Ala Lys Ile Ser Glu Arg Tyr Gly 5055 60 Ser Val Leu Leu Leu Glu Phe Gly Ser Arg Lys Val Leu Leu Val Ser 6570 75 80 Ser Pro Ser Ala Ala Glu Glu Cys Leu Thr Lys Asn Asp Ile Ile Phe85 90 95 Ala Asn Arg Pro Leu Leu Met Ala Gly Lys His Leu Gly Tyr Asn Phe100 105 110 Thr Ser Leu Ala Trp Ser Ser Tyr Gly Asp His Trp Arg Asn LeuArg 115 120 125 Arg Ile Thr Ser Val Glu Met Phe Ser Thr His Arg Leu GlnMet Leu 130 135 140 His Gly Ile Arg Ile Asp Glu Val Lys Ser Met Val LysArg Leu Asn 145 150 155 160 Ser Ser Ala Ile Ala Glu Lys Ser Val Asp MetLys Ser Met Phe Phe 165 170 175 Glu Leu Met Leu Asn Val Met Met Arg ThrIle Ala Gly Lys Arg Tyr 180 185 190 Tyr Gly Glu Asn Val Glu Asp Ile GluGlu Ala Thr Arg Phe Lys Gly 195 200 205 Leu Val Gln Glu Thr Phe Arg IleGly Gly Ala Thr Asn Ile Gly Asp 210 215 220 Phe Leu Pro Ala Leu Lys LeuLeu Val Arg Lys Leu Glu Lys Ser Leu 225 230 235 240 Ile Val Leu Gln GluAsn Arg Asp Glu Phe Met Gln Glu Leu Ile Lys 245 250 255 Asp Cys Arg LysArg Met Glu Lys Glu Gly Thr Val Thr Asp Ser Glu 260 265 270 Ile Glu GlyAsn Lys Lys Cys Leu Ile Glu Val Leu Leu Thr Leu Gln 275 280 285 Glu AsnGlu Pro Glu Tyr Tyr Lys Asp Glu Ile Ile Arg Ser Leu Met 290 295 300 LeuVal Leu Leu Ser Ala Gly Thr Asp Thr Ser Val Gly Thr Met Glu 305 310 315320 Trp Ala Leu Ser Leu Met Leu Asn His Pro Glu Thr Leu Lys Lys Ala 325330 335 Gln Ala Glu Ile Asp Glu His Ile Gly His Glu Arg Leu Val Asp Glu340 345 350 Ser Asp Ile Asn Asn Leu Pro Tyr Leu Arg Cys Ile Ile Asn GluThr 355 360 365 Phe Arg Met Tyr Pro Ala Gly Pro Leu Leu Val Pro His GluSer Ser 370 375 380 Glu Glu Thr Thr Val Gly Gly Tyr Arg Val Pro Gly GlyThr Met Leu 385 390 395 400 Leu Val Asn Leu Trp Ala Ile His Asn Asp ProLys Leu Trp Asp Glu 405 410 415 Pro Arg Lys Phe Lys Pro Glu Arg Phe GluGly Leu Glu Gly Val Arg 420 425 430 Asp Gly Tyr Lys Met Met Pro Phe GlySer Gly Arg Arg Ser Cys Pro 435 440 445 Gly Glu Gly Leu Ala Ile Arg MetVal Ala Leu Ser Leu Gly Cys Ile 450 455 460 Ile Gln Cys Phe Asp Trp GlnArg Leu Gly Glu Gly Leu Val Asp Lys 465 470 475 480 Thr Glu Gly Thr GlyLeu Thr Leu Pro Lys Ala Gln Pro Leu Val Ala 485 490 495 Lys Cys Ser ProArg Pro Ile Met Ala Asn Leu Leu Ser Gln Ile 500 505 510 169 1755 DNANICOTIANATABACUM 169 caacacgctt actatctcct aaatctccac tcaaaaacaaagaagagaaa gatttaaaac 60 taataattat gaaagagatg gtgcaaaaca atatgagcacttctcttctt gaaactttac 120 aagctacgcc catgatattc tacttcatcg tccctctcttctgcttattc cttctctcca 180 aatctcgccg taaacgtttg cctccaggtc caactggctggcctctcatt ggtaacatga 240 tgatgatgga ccagttaact caccgtggcc ttgccaaactagcccaaaaa tatggtggtg 300 tttttcacct taaaatgggt tatgttcaca aaattgtagtctctggtcca gacgaagctc 360 gccaagtatt acaggaacac gacatcatat tttcgaaccgtccagcgacc gtagccataa 420 gttacctaac atatgacagg gcagacatgg cttttgctgactatggactc ttctggcggc 480 agatgagaaa actatgtgta atgaaactct tcagccgcaaacgagctgag tcatgggact 540 cagttcgaga cgaagcggat tccatggtta gaattgtaacaaccaacaca ggcacagctg 600 ttaacttagg tgaacttgtt ttcagtctca ctcgtaatattatctacaga gctgcttttg 660 gaacttgttc tgaagatgga caaggcgagt tcattaaaattatgcaagag ttttcgaagc 720 tatttggtgc tttcaatata gctgatttta ttccatggctagggtgggtt ggtaagcaga 780 gtctaaatat tagacttgct aaggctagag cgtcgcttgatgggttcatt gattcgatta 840 ttgatgacca tattattaga aagaaagctt atgttaatggcaaaaatgat ggaggtgatc 900 gagaaactga tatggtggat gagcttttag ctttttacagtgaggaagca aaagtaactg 960 agtccgaaga tttgcagaat gctatcagac ttactaaggataatatcaaa gctatcatca 1020 tggatgtaat gtttggaggg acagaaacag tggcttctgcaatagaatgg gccatggcag 1080 agcttatgag gagtcctgaa gatcttaaaa aggtacaacaagagctggct aacgttgttg 1140 gactcaacag aaaagttgaa gaatctgact ttgaaaaattaacatactta agatgttgtc 1200 taaaagaaac tctacgactt caccctccaa tccctctcctcctccatgag accgccgagg 1260 aatccaccgt ctccggctac catattccgg caaagtcacatgttattata aattcatttg 1320 ccattgggcg tgacaaaaat tcatgggaag atcctgaaacttataaacca tctaggtttc 1380 tcaaagaagg tgtaccagat tttaaaggag gtaattttgagtttatacca tttgggtcgg 1440 gtcggcggtc ttgccccggt atgcaacttg ggctttatgcattggaaatg gctgtggccc 1500 atcttcttca ttgttttact tgggaattgc cagatggtatgaaaccaagt gagcttaaaa 1560 tggatgatat ttttggactc actgctccaa gagctaatcgactcgtggct gtgcctactc 1620 cacgtttgtt gtgtcccctt tattaattga agaaaaaaggtggggctttt acttgcatca 1680 aagagtggtg cttgtgattt ttccaccttt tggttaaatatacgaattat tatgatatac 1740 gaattcttgg gcaca 1755 170 525 PRTNICOTIANATABACUM 170 Met Lys Glu Met Val Gln Asn Asn Met Ser Thr Ser LeuLeu Glu Thr 1 5 10 15 Leu Gln Ala Thr Pro Met Ile Phe Tyr Phe Ile ValPro Leu Phe Cys 20 25 30 Leu Phe Leu Leu Ser Lys Ser Arg Arg Lys Arg LeuPro Pro Gly Pro 35 40 45 Thr Gly Trp Pro Leu Ile Gly Asn Met Met Met MetAsp Gln Leu Thr 50 55 60 His Arg Gly Leu Ala Lys Leu Ala Gln Lys Tyr GlyGly Val Phe His 65 70 75 80 Leu Lys Met Gly Tyr Val His Lys Ile Val ValSer Gly Pro Asp Glu 85 90 95 Ala Arg Gln Val Leu Gln Glu His Asp Ile IlePhe Ser Asn Arg Pro 100 105 110 Ala Thr Val Ala Ile Ser Tyr Leu Thr TyrAsp Arg Ala Asp Met Ala 115 120 125 Phe Ala Asp Tyr Gly Leu Phe Trp ArgGln Met Arg Lys Leu Cys Val 130 135 140 Met Lys Leu Phe Ser Arg Lys ArgAla Glu Ser Trp Asp Ser Val Arg 145 150 155 160 Asp Glu Ala Asp Ser MetVal Arg Ile Val Thr Thr Asn Thr Gly Thr 165 170 175 Ala Val Asn Leu GlyGlu Leu Val Phe Ser Leu Thr Arg Asn Ile Ile 180 185 190 Tyr Arg Ala AlaPhe Gly Thr Cys Ser Glu Asp Gly Gln Gly Glu Phe 195 200 205 Ile Lys IleMet Gln Glu Phe Ser Lys Leu Phe Gly Ala Phe Asn Ile 210 215 220 Ala AspPhe Ile Pro Trp Leu Gly Trp Val Gly Lys Gln Ser Leu Asn 225 230 235 240Ile Arg Leu Ala Lys Ala Arg Ala Ser Leu Asp Gly Phe Ile Asp Ser 245 250255 Ile Ile Asp Asp His Ile Ile Arg Lys Lys Ala Tyr Val Asn Gly Lys 260265 270 Asn Asp Gly Gly Asp Arg Glu Thr Asp Met Val Asp Glu Leu Leu Ala275 280 285 Phe Tyr Ser Glu Glu Ala Lys Val Thr Glu Ser Glu Asp Leu GlnAsn 290 295 300 Ala Ile Arg Leu Thr Lys Asp Asn Ile Lys Ala Ile Ile MetAsp Val 305 310 315 320 Met Phe Gly Gly Thr Glu Thr Val Ala Ser Ala IleGlu Trp Ala Met 325 330 335 Ala Glu Leu Met Arg Ser Pro Glu Asp Leu LysLys Val Gln Gln Glu 340 345 350 Leu Ala Asn Val Val Gly Leu Asn Arg LysVal Glu Glu Ser Asp Phe 355 360 365 Glu Lys Leu Thr Tyr Leu Arg Cys CysLeu Lys Glu Thr Leu Arg Leu 370 375 380 His Pro Pro Ile Pro Leu Leu LeuHis Glu Thr Ala Glu Glu Ser Thr 385 390 395 400 Val Ser Gly Tyr His IlePro Ala Lys Ser His Val Ile Ile Asn Ser 405 410 415 Phe Ala Ile Gly ArgAsp Lys Asn Ser Trp Glu Asp Pro Glu Thr Tyr 420 425 430 Lys Pro Ser ArgPhe Leu Lys Glu Gly Val Pro Asp Phe Lys Gly Gly 435 440 445 Asn Phe GluPhe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly 450 455 460 Met GlnLeu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 465 470 475 480His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 485 490495 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Arg Ala Asn Arg Leu 500505 510 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro Leu Tyr 515 520 525171 1684 DNA NICOTIANATABACUM 171 tgtgcttgtg agtgtgggag aaggccttcaatatggagat accatattac agcttaaaaa 60 ttgcaatttc ttcatttgca attatctttgtactaagatg ggcatggaaa atcttgaatt 120 atgtgtggtt aaaaccaaaa gaattggagaaatacctcag acagcagggt ttcaaaggaa 180 actcttacaa attcttgttt ggggatatgaaagagatgaa gaaaatgggt gaagaagcta 240 tgtctaagcc aatcaatttc tctcatgacatgatttggcc tagagttatg ccattcatcc 300 acaaaaccat caccaattat ggtaagaattgtattgtgtg gtttgggcca agaccagcag 360 tcctgatcac agacccggaa cttgtaaaggaggtgctaac gaagaatttc gtctatcaga 420 agccgcttgg caatccactc acaaagttggcagcaactgg aattgcaggc tatgaaacag 480 ataaatgggc tacacataga aggcttctcaatcctgcttt tcaccttgac aagttgaagc 540 atatgctacc tgcattccaa tttactgctagtgagatgtt gagcaaattg gagaaagttg 600 tttcaccaaa cggaacagag atagatgtgtggccatattt acaaactttg acaagtgatg 660 ccatttcaag aactgcgttt ggaagtagttatgaagaagg aagaaagatt tttgaccttc 720 aaaaagaaca actttcacta attctagaagtttcacgcac aatatatatt ccaggatgga 780 ggtttttgcc aacgaaaagg aacaaaaggatgaagcaaat atttaatgaa gtacgagcac 840 tggtatttgg aattattaag aaaaggatgagtatgattga aaatggagaa gcacctgatg 900 atttattggg aatattattg gcatccaatttaaaagaaat ccaacaacat ggaaacaaca 960 agaaatttgg tatgagtatt gatgaggtgattgaagagtg taaactcttc tattttgctg 1020 ggcaagagac tacttcatct ttacttgtatggactatgat tttgttgtgc aaatatccta 1080 attggcaaga taaagctaga gaagaggttttgcaagtgtt tgggagtagg gaagttgact 1140 atgacaagtt gaatcagcta aaaatagtaactatgatctt aaacgaggtc ttaaggttgt 1200 atccagcagg atatgtgatt aatcgaatggtaaacaaaga aacaaagtta gggaatttgt 1260 gtttaccagc cggcgtacag ctcgtgttaccaacaatgtt gttgcaacat gatactgaaa 1320 tatggggaga tgatgcaatg gagttcaatccagagagatt tagtgatgga atatccaaag 1380 caacaaaagg aaaacttgtg ttttttccatttagttgggg tccaagaata tgtattgggc 1440 aaaattttgc tatgttagag gctaaaatggcaatggctat gattctgaaa acctatgcat 1500 ttgaactctc tccatcttat gctcatgctcctcatccact actacttcaa cctcaatatg 1560 gtgctcaatt aattttgtac aagttgtagatatggtcaat ttggaacttg ttatggaact 1620 tttatcattg taatcaacca tattgagggaacatggtttg aggttaaatc ctcgtgtgtg 1680 tgtc 1684 172 518 PRTNICOTIANATABACUM 172 Met Glu Ile Pro Tyr Tyr Ser Leu Lys Ile Ala Ile SerSer Phe Ala 1 5 10 15 Ile Ile Phe Val Leu Arg Trp Ala Trp Lys Ile LeuAsn Tyr Val Trp 20 25 30 Leu Lys Pro Lys Glu Leu Glu Lys Tyr Leu Arg GlnGln Gly Phe Lys 35 40 45 Gly Asn Ser Tyr Lys Phe Leu Phe Gly Asp Met LysGlu Met Lys Lys 50 55 60 Met Gly Glu Glu Ala Met Ser Lys Pro Ile Asn PheSer His Asp Met 65 70 75 80 Ile Trp Pro Arg Val Met Pro Phe Ile His LysThr Ile Thr Asn Tyr 85 90 95 Gly Lys Asn Cys Ile Val Trp Phe Gly Pro ArgPro Ala Val Leu Ile 100 105 110 Thr Asp Pro Glu Leu Val Lys Glu Val LeuThr Lys Asn Phe Val Tyr 115 120 125 Gln Lys Pro Leu Gly Asn Pro Leu ThrLys Leu Ala Ala Thr Gly Ile 130 135 140 Ala Gly Tyr Glu Thr Asp Lys TrpAla Thr His Arg Arg Leu Leu Asn 145 150 155 160 Pro Ala Phe His Leu AspLys Leu Lys His Met Leu Pro Ala Phe Gln 165 170 175 Phe Thr Ala Ser GluMet Leu Ser Lys Leu Glu Lys Val Val Ser Pro 180 185 190 Asn Gly Thr GluIle Asp Val Trp Pro Tyr Leu Gln Thr Leu Thr Ser 195 200 205 Asp Ala IleSer Arg Thr Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Lys IlePhe Asp Leu Gln Lys Glu Gln Leu Ser Leu Ile Leu Glu Val 225 230 235 240Ser Arg Thr Ile Tyr Ile Pro Gly Trp Arg Phe Leu Pro Thr Lys Arg 245 250255 Asn Lys Arg Met Lys Gln Ile Phe Asn Glu Val Arg Ala Leu Val Phe 260265 270 Gly Ile Ile Lys Lys Arg Met Ser Met Ile Glu Asn Gly Glu Ala Pro275 280 285 Asp Asp Leu Leu Gly Ile Leu Leu Ala Ser Asn Leu Lys Glu IleGln 290 295 300 Gln His Gly Asn Asn Lys Lys Phe Gly Met Ser Ile Asp GluVal Ile 305 310 315 320 Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly Gln GluThr Thr Ser Ser 325 330 335 Leu Leu Val Trp Thr Met Ile Leu Leu Cys LysTyr Pro Asn Trp Gln 340 345 350 Asp Lys Ala Arg Glu Glu Val Leu Gln ValPhe Gly Ser Arg Glu Val 355 360 365 Asp Tyr Asp Lys Leu Asn Gln Leu LysIle Val Thr Met Ile Leu Asn 370 375 380 Glu Val Leu Arg Leu Tyr Pro AlaGly Tyr Val Ile Asn Arg Met Val 385 390 395 400 Asn Lys Glu Thr Lys LeuGly Asn Leu Cys Leu Pro Ala Gly Val Gln 405 410 415 Leu Val Leu Pro ThrMet Leu Leu Gln His Asp Thr Glu Ile Trp Gly 420 425 430 Asp Asp Ala MetGlu Phe Asn Pro Glu Arg Phe Ser Asp Gly Ile Ser 435 440 445 Lys Ala ThrLys Gly Lys Leu Val Phe Phe Pro Phe Ser Trp Gly Pro 450 455 460 Arg IleCys Ile Gly Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala 465 470 475 480Met Ala Met Ile Leu Lys Thr Tyr Ala Phe Glu Leu Ser Pro Ser Tyr 485 490495 Ala His Ala Pro His Pro Leu Leu Leu Gln Pro Gln Tyr Gly Ala Gln 500505 510 Leu Ile Leu Tyr Lys Leu 515 173 1697 DNA NICOTIANATABACUM 173ccagcaccaa gacatggaga attcctgggt agttttagcc ttaacaggcc ttcttacatt 60agtttttctc tcaaagtttc ttcatagtcc tcgtcgtaaa caaaatcttc caccaggtcc 120aaaaccatgg cctattgttg gcaatataca tcttcttggt tccacccctc acagatccct 180tcacgaactt gcaaaaagat acggagattt aatgctacta aagttcggtt cgcgcaatgt 240ccttatttta tcctccccag atatggctag agaattcttg aaaacaaatg atgccatttg 300ggcttctcgc cctgagcttg ccgctggtaa atatactgct tataattatt gcgacatgac 360atgggcacgt tatggaccct tttggagaca agcaaggagg atctatctca acgagatttt 420caatcctaaa cgtttggatt catttgagta cattcgcata gaggaaaggc ataatttgat 480ttcacgtctt tttgttctct ctgggaagcc aattcttctt agagaccatt taactcggta 540cactcttaca agtataagta gaacagtatt gagtggaaaa tattttagcg agtcacctgg 600ccaaaattca atgataactt tgaaacaatt gcaggatatg cttgataagt ggtttttgct 660taatggtgtg atcaatattg gggactggat accttggctt gctttcttgg atttgcaggg 720ttatgtcaag caaatgaagg agttgcatag gaacttcgac aaatttcata actttgtgct 780agatgatcac aaggctaata ggggagagaa gaactttgtg ccaagagaca tggtcgatgt 840tttgctgcag caagctgagg atcctaatct tgaggtcaaa ctcaccaatg attgtgtcaa 900gggtctaatg caggacttat tggctggcgg cacggacacc tcagcaacaa ccgttgaatg 960ggctttttat gaacttctta gacaacctaa gattatgaag aaagcacaac aagagctaga 1020ccttgtcatt tcacaggaca gatgggttca agaaaaagat tacactcaac tcccttacat 1080tgagtcaatc atcaaggaaa cattgaggct tcacccagta agcaccatgc ttccaccgcg 1140cattgccttg gaggattgtc atgtagcagg ctatgacata cctaaaggta caattttaat 1200tgtgaacact tggagtattg gaagaaattc acagcattgg gagtcaccag aagaattcct 1260tccggagagg tttgaaggga agaatattgg tgtcacagga caacattttg cgctcttgcc 1320atttggcgcg ggccggagaa agtgcccagg atacagtctt gggattcgta taattagggc 1380aactttagct aacttgttgc atggattcaa ctggagattg cctaatggta tgagtccaga 1440agacattagc atggaagaga tttatgggct aattacacac cccaaagtcg cacttgacgt 1500gatgatggag cctcgacttc ccaaccatct ttacaaatag tggataatta aaaccattaa 1560aatcgttttg ttatatgcat gtctcatatt tgtagtggtc aaaatgtttg ttttctatca 1620tggatgttca gtgcgaggtt gggaatttca agtcattaac gtgtgaaaat attttaaatt 1680taaaaaaaaa aaaaaaa 1697 174 508 PRT NICOTIANATABACUM 174 Met Glu Asn SerTrp Val Val Leu Ala Leu Thr Gly Leu Leu Thr Leu 1 5 10 15 Val Phe LeuSer Lys Phe Leu His Ser Pro Arg Arg Lys Gln Asn Leu 20 25 30 Pro Pro GlyPro Lys Pro Trp Pro Ile Val Gly Asn Ile His Leu Leu 35 40 45 Gly Ser ThrPro His Arg Ser Leu His Glu Leu Ala Lys Arg Tyr Gly 50 55 60 Asp Leu MetLeu Leu Lys Phe Gly Ser Arg Asn Val Leu Ile Leu Ser 65 70 75 80 Ser ProAsp Met Ala Arg Glu Phe Leu Lys Thr Asn Asp Ala Ile Trp 85 90 95 Ala SerArg Pro Glu Leu Ala Ala Gly Lys Tyr Thr Ala Tyr Asn Tyr 100 105 110 CysAsp Met Thr Trp Ala Arg Tyr Gly Pro Phe Trp Arg Gln Ala Arg 115 120 125Arg Ile Tyr Leu Asn Glu Ile Phe Asn Pro Lys Arg Leu Asp Ser Phe 130 135140 Glu Tyr Ile Arg Ile Glu Glu Arg His Asn Leu Ile Ser Arg Leu Phe 145150 155 160 Val Leu Ser Gly Lys Pro Ile Leu Leu Arg Asp His Leu Thr ArgTyr 165 170 175 Thr Leu Thr Ser Ile Ser Arg Thr Val Leu Ser Gly Lys TyrPhe Ser 180 185 190 Glu Ser Pro Gly Gln Asn Ser Met Ile Thr Leu Lys GlnLeu Gln Asp 195 200 205 Met Leu Asp Lys Trp Phe Leu Leu Asn Gly Val IleAsn Ile Gly Asp 210 215 220 Trp Ile Pro Trp Leu Ala Phe Leu Asp Leu GlnGly Tyr Val Lys Gln 225 230 235 240 Met Lys Glu Leu His Arg Asn Phe AspLys Phe His Asn Phe Val Leu 245 250 255 Asp Asp His Lys Ala Asn Arg GlyGlu Lys Asn Phe Val Pro Arg Asp 260 265 270 Met Val Asp Val Leu Leu GlnGln Ala Glu Asp Pro Asn Leu Glu Val 275 280 285 Lys Leu Thr Asn Asp CysVal Lys Gly Leu Met Gln Asp Leu Leu Ala 290 295 300 Gly Gly Thr Asp ThrSer Ala Thr Thr Val Glu Trp Ala Phe Tyr Glu 305 310 315 320 Leu Leu ArgGln Pro Lys Ile Met Lys Lys Ala Gln Gln Glu Leu Asp 325 330 335 Leu ValIle Ser Gln Asp Arg Trp Val Gln Glu Lys Asp Tyr Thr Gln 340 345 350 LeuPro Tyr Ile Glu Ser Ile Ile Lys Glu Thr Leu Arg Leu His Pro 355 360 365Val Ser Thr Met Leu Pro Pro Arg Ile Ala Leu Glu Asp Cys His Val 370 375380 Ala Gly Tyr Asp Ile Pro Lys Gly Thr Ile Leu Ile Val Asn Thr Trp 385390 395 400 Ser Ile Gly Arg Asn Ser Gln His Trp Glu Ser Pro Glu Glu PheLeu 405 410 415 Pro Glu Arg Phe Glu Gly Lys Asn Ile Gly Val Thr Gly GlnHis Phe 420 425 430 Ala Leu Leu Pro Phe Gly Ala Gly Arg Arg Lys Cys ProGly Tyr Ser 435 440 445 Leu Gly Ile Arg Ile Ile Arg Ala Thr Leu Ala AsnLeu Leu His Gly 450 455 460 Phe Asn Trp Arg Leu Pro Asn Gly Met Ser ProGlu Asp Ile Ser Met 465 470 475 480 Glu Glu Ile Tyr Gly Leu Ile Thr HisPro Lys Val Ala Leu Asp Val 485 490 495 Met Met Glu Pro Arg Leu Pro AsnHis Leu Tyr Lys 500 505 175 1631 DNA NICOTIANATABACUM 175 actgttcaaatcacagtaac agcatcttgt gctgccataa taattactct agtggtgtgt 60 atatggagagtgctgaattg ggtttggttc agaccaaaga agctggaaaa gctactgagg 120 aaacaaggtctcaaaggcaa ttcctacagg attttgtatg gggatatgaa ggagctttct 180 ggtatgattaaggaagctaa ctccaaaccc atgaatcttt ctgatgatat tgccccaaga 240 ttggtccctttctttcttga taccatcaag aaatatggga aaaaatcctt tgtatggttg 300 ggtccaaaaccgctggtttt tgtcatggac cccgagctta taaaggaagt attctccaaa 360 aactatctgtatcaaaagcc tcattcaaat ccattaacca agttactggc acaaggactt 420 gtaagccaagaggaagacaa atgggccaaa catagaaaaa tcgtcactcc tgccttccac 480 ctggagaagctaaagcatat gcttccagct ttttgtttga gctgtactga gatgctgagc 540 aaatgggaagacattgttgc agttgagggc tcacatgaga tagatatatg gcctggcctt 600 caacaattaactagtgatgt gatctctcgg acagcctttg gcagtagcta tgaagcaggt 660 agaaggatatttgaacttca aaaggaacaa gctcaatttc ttatggaagc tatacgctcc 720 gtttatattccaggctggag gtttttgcca acaaagagga acagaagaat gaaggaaatt 780 gaaaaggatgttcaagcctt agttagaggt attattgata aaagagtaaa gtcaatgaaa 840 gcaggagaggtgaataatga ggatctgctt ggtatattgc tggaatctaa ttttaaagaa 900 attgaacagcatggaaacaa ggattttgga atgagcattg aagaagtcat tcaagaatgc 960 aagttattctattttgctgg ccaagaaact acatcagtgt tgcttgtatg gactctaata 1020 ttgctgagcaggcatcagga ttggcaagca ctggccagag aagaggtgtt gcaagtcttt 1080 gggaatcagaaaccagattt tgatggatta aatcgtctaa aaattgttac aatgatcttg 1140 tacgagtctttaaggctcta tcccccagta gtgacactta cccgaaggcc taaggaagac 1200 actgtattaggagatgtatc tctaccagca ggtgtgttaa tctccttacc agtgatctta 1260 ttgcatcacgacgaagagat atggggtaaa gatgcaaaga agttcaagcc agagagattc 1320 agagatggagtctcaagtgc aacaaagggt caagtcactt ttttcccatt tacttggggt 1380 cccagaatatgcattggaca aaattttgcc atgttagaag caaagactac tttggctatg 1440 atcctacaacgcttctcctt tgaactgtct ccatcttatg cacatgctcc tcagtccata 1500 ataactttgcaaccccagta tggtgctcca cttattttgc ataaaatata gtttattact 1560 tgtaagtagtgtctcgtttt atgttaagca tgagtccaaa atgttaaggc ttgtagaact 1620 gcaaaatggg a1631 176 461 PRT NICOTIANATABACUM 176 Met Lys Glu Leu Ser Gly Met IleLys Glu Ala Asn Ser Lys Pro Met 1 5 10 15 Asn Leu Ser Asp Asp Ile AlaPro Arg Leu Val Pro Phe Phe Leu Asp 20 25 30 Thr Ile Lys Lys Tyr Gly LysLys Ser Phe Val Trp Leu Gly Pro Lys 35 40 45 Pro Leu Val Phe Val Met AspPro Glu Leu Ile Lys Glu Val Phe Ser 50 55 60 Lys Asn Tyr Leu Tyr Gln LysPro His Ser Asn Pro Leu Thr Lys Leu 65 70 75 80 Leu Ala Gln Gly Leu ValSer Gln Glu Glu Asp Lys Trp Ala Lys His 85 90 95 Arg Lys Ile Val Thr ProAla Phe His Leu Glu Lys Leu Lys His Met 100 105 110 Leu Pro Ala Phe CysLeu Ser Cys Thr Glu Met Leu Ser Lys Trp Glu 115 120 125 Asp Ile Val AlaVal Glu Gly Ser His Glu Ile Asp Ile Trp Pro Gly 130 135 140 Leu Gln GlnLeu Thr Ser Asp Val Ile Ser Arg Thr Ala Phe Gly Ser 145 150 155 160 SerTyr Glu Ala Gly Arg Arg Ile Phe Glu Leu Gln Lys Glu Gln Ala 165 170 175Gln Phe Leu Met Glu Ala Ile Arg Ser Val Tyr Ile Pro Gly Trp Arg 180 185190 Phe Leu Pro Thr Lys Arg Asn Arg Arg Met Lys Glu Ile Glu Lys Asp 195200 205 Val Gln Ala Leu Val Arg Gly Ile Ile Asp Lys Arg Val Lys Ser Met210 215 220 Lys Ala Gly Glu Val Asn Asn Glu Asp Leu Leu Gly Ile Leu LeuGlu 225 230 235 240 Ser Asn Phe Lys Glu Ile Glu Gln His Gly Asn Lys AspPhe Gly Met 245 250 255 Ser Ile Glu Glu Val Ile Gln Glu Cys Lys Leu PheTyr Phe Ala Gly 260 265 270 Gln Glu Thr Thr Ser Val Leu Leu Val Trp ThrLeu Ile Leu Leu Ser 275 280 285 Arg His Gln Asp Trp Gln Ala Leu Ala ArgGlu Glu Val Leu Gln Val 290 295 300 Phe Gly Asn Gln Lys Pro Asp Phe AspGly Leu Asn Arg Leu Lys Ile 305 310 315 320 Val Thr Met Ile Leu Tyr GluSer Leu Arg Leu Tyr Pro Pro Val Val 325 330 335 Thr Leu Thr Arg Arg ProLys Glu Asp Thr Val Leu Gly Asp Val Ser 340 345 350 Leu Pro Ala Gly ValLeu Ile Ser Leu Pro Val Ile Leu Leu His His 355 360 365 Asp Glu Glu IleTrp Gly Lys Asp Ala Lys Lys Phe Lys Pro Glu Arg 370 375 380 Phe Arg AspGly Val Ser Ser Ala Thr Lys Gly Gln Val Thr Phe Phe 385 390 395 400 ProPhe Thr Trp Gly Pro Arg Ile Cys Ile Gly Gln Asn Phe Ala Met 405 410 415Leu Glu Ala Lys Thr Thr Leu Ala Met Ile Leu Gln Arg Phe Ser Phe 420 425430 Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Gln Ser Ile Ile Thr Leu 435440 445 Gln Pro Gln Tyr Gly Ala Pro Leu Ile Leu His Lys Ile 450 455 460177 1572 DNA NICOTIANATABACUM 177 atttatctct gaaaatgcaa ttcttcagcttggtttccat tttcctattc ctatctttcc 60 tatttttgtt gaggaaatgg aagaactccaatagccaaag caaaaaattg ccaccaggtc 120 catggaaaat accaatacta ggaagtatgcttcatatgat tggtggagaa ccgcaccatg 180 tccttagaga tttagccaaa aaagatggaccacttatgca ccttcagtta ggtgaaattt 240 ctgcagttgt ggttacttct agggacatggcaaaagaagt gctaaaaact catgacgtcg 300 tttttgcatc taggcctaaa attgtagccatggacattat ctgttataac cagtccgaca 360 ttgcctttag cccttatggc gaccactggagacaaatgcg taaaatttgt gtcatggaac 420 ttctcaatgc aaagaatgtt cggtctttcagctccatcag acgtgatgaa gtcgttcgtc 480 tcattgactc tatccggtca gattcttcttcaggtgagct agttaatttt acgcagagga 540 tcatttggtt tgcaagctcc atgacgtgtagatcagcatt tgggcaagta ctcaaggggc 600 aagacatatt tgccaaaaag atcagagaagtaataggatt agcagaaggc tttgatgtgg 660 tagacatctt ccctacatac aagtttcttcatgttctcag tgggatgaag cgtaaacttt 720 tgaatgccca ccttaaggta gacgccattgttgaggatgt catcaacgag cacaagaaaa 780 atcttgcagc tggcaagagt aatggcgcattaggaggcga agatctaatt gatgtcctac 840 tgagacttat gaatgacaca agtcttcaatttcccatcac caacgacaat atcaaagctg 900 ttgttgttga catgtttgct gccggaacagaaacttcatc aacaacaact gtatgggcca 960 tggctgaaat gatgaagaat ccaagtgtattcgccaaagc tcaagcagaa gtgcgagaag 1020 cctttaggga caaagtatct tttgatgaaaatgatgtgga ggagctgaaa tacttaaagt 1080 tagtcattaa agaaactttg agacttcatccaccgtctcc acttttggtc ccaagagaat 1140 gcagggaaga tacggatata aacggctacactattcctgc aaagaccaaa gttatggtta 1200 atgtttgggc attgggaaga gatccaaaatattgggatga cgcggaaagc tttaagccag 1260 agagatttga gcaatgttct gtagatatttttggtaataa ttttgagttt cttccctttg 1320 gcgggggacg gagaatttgt cctggaatgtcatttggttt agctaatctt tacttaccat 1380 tggctcaatt actctatcac tttgactggaaactcccaac cggaatcaag ccaagagact 1440 tggacttgac cgaattatcg ggaataactattgctagaaa gggtgacctt tacttaaatg 1500 ctactcctta tcaaccttct cgagagtaatttactattgg cataaacatt ttaaatttcc 1560 ttcatcaacc tc 1572 178 504 PRTNICOTIANATABACUM 178 Met Gln Phe Phe Ser Leu Val Ser Ile Phe Leu Phe LeuSer Phe Leu 1 5 10 15 Phe Leu Leu Arg Lys Trp Lys Asn Ser Asn Ser GlnSer Lys Lys Leu 20 25 30 Pro Pro Gly Pro Trp Lys Ile Pro Ile Leu Gly SerMet Leu His Met 35 40 45 Ile Gly Gly Glu Pro His His Val Leu Arg Asp LeuAla Lys Lys Asp 50 55 60 Gly Pro Leu Met His Leu Gln Leu Gly Glu Ile SerAla Val Val Val 65 70 75 80 Thr Ser Arg Asp Met Ala Lys Glu Val Leu LysThr His Asp Val Val 85 90 95 Phe Ala Ser Arg Pro Lys Ile Val Ala Met AspIle Ile Cys Tyr Asn 100 105 110 Gln Ser Asp Ile Ala Phe Ser Pro Tyr GlyAsp His Trp Arg Gln Met 115 120 125 Arg Lys Ile Cys Val Met Glu Leu LeuAsn Ala Lys Asn Val Arg Ser 130 135 140 Phe Ser Ser Ile Arg Arg Asp GluVal Val Arg Leu Ile Asp Ser Ile 145 150 155 160 Arg Ser Asp Ser Ser SerGly Glu Leu Val Asn Phe Thr Gln Arg Ile 165 170 175 Ile Trp Phe Ala SerSer Met Thr Cys Arg Ser Ala Phe Gly Gln Val 180 185 190 Leu Lys Gly GlnAsp Ile Phe Ala Lys Lys Ile Arg Glu Val Ile Gly 195 200 205 Leu Ala GluGly Phe Asp Val Val Asp Ile Phe Pro Thr Tyr Lys Phe 210 215 220 Leu HisVal Leu Ser Gly Met Lys Arg Lys Leu Leu Asn Ala His Leu 225 230 235 240Lys Val Asp Ala Ile Val Glu Asp Val Ile Asn Glu His Lys Lys Asn 245 250255 Leu Ala Ala Gly Lys Ser Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile 260265 270 Asp Val Leu Leu Arg Leu Met Asn Asp Thr Ser Leu Gln Phe Pro Ile275 280 285 Thr Asn Asp Asn Ile Lys Ala Val Val Val Asp Met Phe Ala AlaGly 290 295 300 Thr Glu Thr Ser Ser Thr Thr Thr Val Trp Ala Met Ala GluMet Met 305 310 315 320 Lys Asn Pro Ser Val Phe Ala Lys Ala Gln Ala GluVal Arg Glu Ala 325 330 335 Phe Arg Asp Lys Val Ser Phe Asp Glu Asn AspVal Glu Glu Leu Lys 340 345 350 Tyr Leu Lys Leu Val Ile Lys Glu Thr LeuArg Leu His Pro Pro Ser 355 360 365 Pro Leu Leu Val Pro Arg Glu Cys ArgGlu Asp Thr Asp Ile Asn Gly 370 375 380 Tyr Thr Ile Pro Ala Lys Thr LysVal Met Val Asn Val Trp Ala Leu 385 390 395 400 Gly Arg Asp Pro Lys TyrTrp Asp Asp Ala Glu Ser Phe Lys Pro Glu 405 410 415 Arg Phe Glu Gln CysSer Val Asp Ile Phe Gly Asn Asn Phe Glu Phe 420 425 430 Leu Pro Phe GlyGly Gly Arg Arg Ile Cys Pro Gly Met Ser Phe Gly 435 440 445 Leu Ala AsnLeu Tyr Leu Pro Leu Ala Gln Leu Leu Tyr His Phe Asp 450 455 460 Trp LysLeu Pro Thr Gly Ile Lys Pro Arg Asp Leu Asp Leu Thr Glu 465 470 475 480Leu Ser Gly Ile Thr Ile Ala Arg Lys Gly Asp Leu Tyr Leu Asn Ala 485 490495 Thr Pro Tyr Gln Pro Ser Arg Glu 500 179 1633 DNA NICOTIANATABACUM179 ataatgcttt ctcccataga agccattgta ggactagtaa ccttcacatt tctcttcttc 60ttcctatgga caaaaaaatc tcaaaaacct tcaaaaccct taccaccgaa aatccccgga 120ggatggccgg taatcggcca tcttttccac ttcaatgacg acggcgacga ccgtccatta 180gctcgaaaac tcggagactt agctgacaaa tacggccccg ttttcacttt tcggctaggc 240cttccccttg tcttagttgt aagcagttac gaagctgtaa aagactgttt ctctacaaat 300gacgccattt tttccaatcg tccagctttt ctttacggcg attaccttgg ctacaataat 360gccatgctat ttttggccaa ttacggacct tactggcgaa aaaatcgaaa attagttatt 420caggaagttc tctccgctag tcgtctcgaa aaattcaaac acgtgagatt tgcaagaatt 480caagcgagca ttaagaattt atatactcga attgatggaa attcgagtac gataaattta 540actgattggt tagaagaatt gaattttggt ctgatcgtga agatgatcgc tggaaaaaat 600tatgaatccg gtaaaggaga tgaacaagtg gagagattta agaaagcgtt taaggatttt 660atgattttat caatggagtt tgtgttatgg gatgcatttc caattccatt atttaaatgg 720gtggattttc aagggcatgt taaggctatg aaaaggactt ttaaagatat agattctgtt 780tttcagaatt ggttagggga acatattaat aaaagagaaa aaatggaggt taatgcagaa 840gggaatgaac aagatttcat tgatgtggtg ctttcaaaaa tgagtaatga atatcttggt 900gaaggttact ctcgtgatac tgtcattaaa gcaacggtgt ttagtttggt cttggatgca 960gcagacacag ttgctcttca cataaattgg ggaatggcat tattgataaa caatcaaaag 1020gccttgacga aagcacaaga agagatagac acaaaagttg gtaaggacag atgggtagaa 1080gagagtgata ttaaggattt ggtatacctc caagctattg ttaaagaagt gttacgatta 1140tatccaccag gacctttgtt agtaccacac gaaaatgtag aagattgtgt tgttagtgga 1200tatcacattc ctaaagggac aagattattc gcaaacgtca tgaaactgct acgtgatcct 1260aaactctggc ctgatcctga tactttcgat ccagagagat tcattgctac tgatattgac 1320tttcgtggtc agtactataa gtatatcccg tttggttctg gaagacgatc ttgtccaggg 1380atgacttatg cattgcaagt ggaacactta acaatggcac atttgatcca aggtttcaat 1440tacagaactc caaatgacga gcccttggat atgaaggaag gtgcaggcat aactatacgt 1500aaggtaaatc ctgtggaact gataatagcg cctcgcctgg cacctgagct ttattaaaac 1560ctaagatctt tcatcttggt tgatcattgt ataatactcc taaatggata ttcatttacc 1620ttttatcaat taa 1633 180 517 PRT NICOTIANATABACUM 180 Met Leu Ser Pro IleGlu Ala Ile Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Phe Phe PheLeu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro 20 25 30 Leu Pro Pro LysIle Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn AspAsp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala AspLys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu ValLeu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe 85 90 95 Ser Thr AsnAsp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Asp TyrLeu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 ProTyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln 145 150155 160 Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu IleVal 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly AspGlu Gln 195 200 205 Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met IleLeu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro LeuPhe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Val Lys Ala Met LysArg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu GlyGlu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val Asn Ala Glu GlyAsn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Met Ser AsnGlu Tyr Leu Gly Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys AlaThr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val AlaLeu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn 325 330 335 Asn Gln LysAla Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Gly LysAsp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 LeuGln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr 385 390395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Leu405 410 415 Arg Asp Pro Lys Leu Trp Pro Asp Pro Asp Thr Phe Asp Pro GluArg 420 425 430 Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr LysTyr Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met ThrTyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile GlnGly Phe Asn Tyr 465 470 475 480 Arg Thr Pro Asn Asp Glu Pro Leu Asp MetLys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val GluLeu Ile Ile Ala Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 181 1585DNA NICOTIANATABACUM 181 aatccataat gctttctccc atagaagcca ttgtaggactagtaaccttc acatttctct 60 tcttcttcct atggacaaaa aaatctcaaa aaccttcaaaacccttacca ccgaaaatcc 120 ccggaggatg gccggtaatc ggccatcttt tccacttcaatgacgacggc gacgaccgtc 180 cattagctcg aaaactcgga gacttagctg acaaatacggccccgttttc acttttcggc 240 taggccttcc ccttgtctta gttgtaagca gttacgaagctgtaaaagac tgtttctcta 300 caaatgacgc cattttttcc aatcgtccag cttttctttacggcgattac cttggctaca 360 ataatgccat gctatttttg gccaattacg gaccttactggcgaaaaaat cgaaaattag 420 ttattcagga agttctctcc gctagtcgtc tcgaaaaattcaaacacgtg agatttgcaa 480 gaattcaagc gagcattaag aatttatata ctcgaattgatggaaattcg agtacgataa 540 atttaactga ttggttagaa gaattgaatt ttggtctgatcgtgaagatg atcgctggaa 600 aaaattatga atccggtaaa ggagatgaac aagtggagagatttaagaaa gcgtttaagg 660 attttatgat tttatcaatg gagtttgtgt tatgggatgcatttccaatt ccattattta 720 aatgggtgga ttttcaaggg catgttaagg ctatgaaaaggacttttaaa gatatagatt 780 ctgtttttca gaattggtta gaggaacata ttaataaaagagaaaaaatg gaggttaatg 840 cagaagggaa tgaacaagat ttcattgatg tggtgctttcaaaaatgagt aatgaatatc 900 ttggtgaagg ttactctcgt gatactgtca ttaaagcaacggtgtttagt ttggtcttgg 960 atgcagcaga cacagttgct cttcacataa attggggaatggcattattg ataaacaatc 1020 aaaaggcctt gacgaaagca caagaagaga tagacacaaaagttggtaag gacagatggg 1080 tagaagagag tgatattaag gatttggtat acctccaagctattgttaaa gaagtgttac 1140 gattatatcc accaggacct ttgttagtac cacacgaaaatgtagaagat tgtgttgtta 1200 gtggatatca cattcctaaa gggacaagat tattcgcaaacgtcatgaaa ctgcaacgtg 1260 atcctaaact ctggtctgat cctgatactt tcgatccagagagattcatt gctactgata 1320 ttgactttcg tggtcagtac tataagtata tcccgtttggttctggaaga cgatcttgtc 1380 cagggatgac ttatgcattg caagtggaac acttaacaatggcacatttg atccaaggtt 1440 tcaattacag aactccaaat gacgagccct tggatatgaaggaaggtgca ggcataacta 1500 tacgtaaggt aaatcctgtg gaactgataa tagcgcctcgcctggcacct gagctttatt 1560 aaaacctaag atcatcttgc ttgat 1585 182 517 PRTNICOTIANATABACUM 182 Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val ThrPhe Thr Phe 1 5 10 15 Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln LysPro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val IleGly His Leu Phe 35 40 45 His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu AlaArg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr PheArg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu AlaVal Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg ProAla Phe Leu Tyr Gly 100 105 110 Asp Tyr Leu Gly Tyr Asn Asn Ala Met LeuPhe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys LeuVal Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe LysHis Val Arg Phe Ala Arg Ile Gln 145 150 155 160 Ala Ser Ile Lys Asn LeuTyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr AspTrp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile AlaGly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu ArgPhe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met 210 215 220 Glu PheVal Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260265 270 Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr SerArg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu AspAla Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met AlaLeu Leu Ile Asn 325 330 335 Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu GluIle Asp Thr Lys Val 340 345 350 Gly Lys Asp Arg Trp Val Glu Glu Ser AspIle Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Val LeuArg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn ValGlu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly ThrArg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys LeuTrp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala ThrAsp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile 435 440 445 Pro Phe GlySer Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln ValGlu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu 500505 510 Ala Pro Glu Leu Tyr 515 183 1620 DNA NICOTIANATABACUM 183ctaaaactcc ataatggttt ctcccgtaga agccattgta ggactagtaa cccttacact 60tctcttctac ttcctatggc ccaaaaaatt tcaaatacct tcaaaaccat taccaccgaa 120aattcccgga gggtggccgg taatcggcca tcttttctac ttcgatgatg acggcgacga 180ccgtccatta gctcgaaaac tcggagactt agctgacaaa tacggcccgg ttttcacttt 240ccggctaggc cttccgcttg tgttaattgt aagcagttac gaagctgtaa aagactgctt 300ctctacaaat gacgccattt tctccaatcg tccagctttt ctttacggtg aataccttgg 360ctacaataat gccatgctat ttttgacaaa atacggacct tattggcgaa aaaatagaaa 420attagtcatt caggaagttc tctctgctag tcgtctcgaa aaattgaagc acgtgagatt 480tggtaaaatt caaacgagca ttaagagttt atacactcga attgatggaa attcgagtac 540gataaatcta actgattggt tagaagaatt gaattttggt ctgatcgtga aaatgatcgc 600tgggaaaaat tatgaatccg gtaaaggaga tgaacaagtg gagagattta ggaaagcgta 660taaggatttt ataattttat caatggagtt tgtgttatgg gatgcttttc caattccatt 720gttcaaatgg gtggattttc aaggctatgt taaggccatg aaaaggacat ttaaggatat 780agattctgtt tttcagaatt ggttagagga acatgtcaag aaaagagaaa aaatggaggt 840taatgcacaa gggaatgaac aagatttcat tgatgtggtg ctttcaaaaa tgagtaatga 900atatcttgat gaaggttact ctcgtgatac tgtcataaaa gcaacagtgt ttagtttggt 960cttggatgct gcggacacag ttgctcttca catgaattgg ggaatggcat tactgataaa 1020caatcaacat gccttgaaga aagcacaaga agagatcgat aagaaagttg gtaaggaaag 1080atgggtagaa gagagtgata ttaaggattt ggtctacctc caagctattg ttaaagaagt 1140gttacgatta tatccaccag gacctttatt agtacctcat gaaaatgtag aggattgtgt 1200tgttagtgga tatcacattc ctaaagggac tagactattc gcgaacgtta tgaaattgca 1260gcgcgatcct aaactctggt caaatcctga taagtttgat ccagagagat tcttcgctga 1320tgatattgac taccgtggtc agcactatga gtttatccca tttggttctg gaagacgatc 1380ttgtccgggg atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca 1440gggtttcaat tacaaaactc caaatgacga gcccttggat atgaaggaag gtgcaggatt 1500aactatacgt aaagtaaatc ctgtagaagt gacaattacg gctcgcctgg cacctgagct 1560ttattaaaac cttagatgtt ttatcttgat tgtactaata tatatatgca gaaaaaattg 1620184 517 PRT NICOTIANATABACUM 184 Met Val Ser Pro Val Glu Ala Ile Val GlyLeu Val Thr Leu Thr Leu 1 5 10 15 Leu Phe Tyr Phe Leu Trp Pro Lys LysPhe Gln Ile Pro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly TrpPro Val Ile Gly His Leu Phe 35 40 45 Tyr Phe Asp Asp Asp Gly Asp Asp ArgPro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro ValPhe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Ile Val Ser SerTyr Glu Ala Val Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe SerAsn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Glu Tyr Leu Gly Tyr Asn AsnAla Met Leu Phe Leu Thr Lys Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys AsnArg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu GluLys Leu Lys His Val Arg Phe Gly Lys Ile Gln 145 150 155 160 Thr Ser IleLys Ser Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile AsnLeu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 LysMet Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205Val Glu Arg Phe Arg Lys Ala Tyr Lys Asp Phe Ile Ile Leu Ser Met 210 215220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225230 235 240 Asp Phe Gln Gly Tyr Val Lys Ala Met Lys Arg Thr Phe Lys AspIle 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Val Lys LysArg Glu 260 265 270 Lys Met Glu Val Asn Ala Gln Gly Asn Glu Gln Asp PheIle Asp Val 275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Asp GluGly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser LeuVal Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Met Asn TrpGly Met Ala Leu Leu Ile Asn 325 330 335 Asn Gln His Ala Leu Lys Lys AlaGln Glu Glu Ile Asp Lys Lys Val 340 345 350 Gly Lys Glu Arg Trp Val GluGlu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val LysGlu Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro HisGlu Asn Val Glu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile ProLys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg AspPro Lys Leu Trp Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg 420 425 430 PhePhe Ala Asp Asp Ile Asp Tyr Arg Gly Gln His Tyr Glu Phe Ile 435 440 445Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455460 Gln Val Glu His Leu Thr Ile Ala His Leu Ile Gln Gly Phe Asn Tyr 465470 475 480 Lys Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala GlyLeu 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Val Thr Ile Thr AlaArg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 185 1623 DNANICOTIANATABACUM 185 cgaggctccc caccaaaaaa tcatttctct cgtctaaaatggatcttctc ttactagaga 60 agaccttaat tggtcttttc tttgccattt taatcgctttaattgtctct aaacttcgtt 120 caaagcgttt taagcttcct ccaggaccaa ttccagtaccagtttttggt aattggcttc 180 aagttggtga tgatttaaac cacagaaatc ttactgattatgccaaaaaa tttggcgatc 240 ttttcttgtt aagaatgggt caacgtaact tagttgttgtgtcatctcct gaattagcta 300 aagaagtttt acacacacaa ggtgttgaat ttggttcaagaacaagaaat gttgtgtttg 360 atatttttac tggaaaaggt caagatatgg tttttactgtatatggtgaa cattggagaa 420 aaatgaggag aattatgact gtaccatttt ttactaataaagttgtgcaa cagtatagag 480 gggggtggga gtttgaggtg gcaagtgtaa ttgaggatgtgaaaaaaaat cctgaatctg 540 ctactaatgg gatcgtatta aggaggagat tacaattaatgatgtataat aatatgttta 600 ggattatgtt tgataggaga tttgagagtg aagatgatcctttgtttgtt aagcttaagg 660 ctttgaatgg tgaaaggagt agattggctc aaagttttgagtataattat ggtgatttta 720 ttccaatttt gaggcctttt ttgagaggtt atttgaagatctgtaaagaa gttaaggaga 780 agaggctgca gcttttcaaa gattactttg ttgatgaaagaaagaagctt tcaaatacca 840 agagctcgga cagcaatgcc ctaaaatgtg cgattgatcacattcttgag gctcaacaga 900 agggagagat caatgaggac aacgttcttt acattgttgaaaacatcaat gttgctgcaa 960 ttgaaacaac attatggtca attgagtggg gtatcgccgagctagtcaac caccctcaca 1020 tccaaaagaa actgcgcgac gagattgaca cagttcttggaccaggagtg caagtgactg 1080 aaccagacac ccacaagctt ccataccttc aggctgtgatcaaggaggca cttcgtctcc 1140 gtatggcaat tcctctatta gtcccacaca tgaaccttcacgacgcaaag cttggcgggt 1200 ttgatattcc agcagagagc aaaatcttgg ttaacgcttggtggttagct aacaacccgg 1260 ctcattggaa gaaacccgaa gagttcagac ccgagaggttctttgaagag gagaagcatg 1320 ttgaggccaa tggcaatgac ttcagatatc ttccgtttggcgttggtagg aggagctgcc 1380 ctggaattat acttgcattg ccaattcttg gcatcactttgggacgtttg gttcagaact 1440 ttgagctgtt gcctcctcca ggccagtcga agctcgacaccacagagaaa ggtggacagt 1500 tcagtctcca cattttgaag cattccacca ttgtgttgaaaccaaggtct ttctgaactt 1560 tgtgatctta ttaattaagg ggttctgaag aaatttgatagtgttggata ttaagggcga 1620 att 1623 186 505 PRT NICOTIANATABACUM 186 MetAsp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15Ile Leu Ile Ala Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys 20 25 30Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys 50 55 60Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 7580 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 9095 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val ValGln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val IleGlu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly IleVal Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met PheArg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro LeuPhe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu AlaGln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu ArgPro Phe Leu Arg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu Val LysGlu Lys Arg Leu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp Glu ArgLys Lys Leu Ser Asn Thr Lys Ser Ser Asp Ser 260 265 270 Asn Ala Leu LysCys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly Glu IleAsn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 Val AlaAla Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330335 Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His 340345 350 Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Ala Leu Arg Leu Arg355 360 365 Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp AlaLys 370 375 380 Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu ValAsn Ala 385 390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys LysPro Glu Glu Phe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys HisVal Glu Ala Asn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly ValGly Arg Arg Ser Cys Pro 435 440 445 Gly Ile Ile Leu Ala Leu Pro Ile LeuGly Ile Thr Leu Gly Arg Leu 450 455 460 Val Gln Asn Phe Glu Leu Leu ProPro Pro Gly Gln Ser Lys Leu Asp 465 470 475 480 Thr Thr Glu Lys Gly GlyGln Phe Ser Leu His Ile Leu Lys His Ser 485 490 495 Thr Ile Val Leu LysPro Arg Ser Phe 500 505 187 1667 DNA NICOTIANATABACUM 187 caacacgcttactatctcct aaatctccac tcaaaaacaa agaagagaaa gatttaaaac 60 taataattatgaaagagatg gtgcaaaaca atatgagcac ttctcttctt gaaactttac 120 aagctacgcccatgatattc tacttcatcg tccctctctt ctgcttattc cttctctcca 180 aatctcgccgtaaacgtttg cctccaggtc caactggctg gcctctcatt ggtaacatga 240 tgatgatggaccagttaact caccgtggcc ttgccaaact agcccaaaaa tatggtggtg 300 tttttcaccttaaaatgggt tatgttcaca aaattgtagt ctctggtcca gacgaagctc 360 gccaagtattacaggaacac gacatcatat tttcgaaccg tccagcgacc gtagccataa 420 gttacctaacatatgacagg gcagacatgg cttttgctga ctatggactc ttctggcggc 480 agatgagaaaactatgtgta atgaaactct tcagccgcaa acgagctgag tcatgggact 540 cagttcgagacgaagcggat tccatggtta gaattgtaac aaccaacaca ggcacagctg 600 ttaacttaggtgaacttgtt ttcagtctca ctcgtaatat tatctacaga gctgcttttg 660 gaacttgttctgaagatgga caaggcgagt tcattgaaat tatgcaagag ttttcgaagc 720 tatttggcgctttcaatata gctgatttta ttccatggct agggtgggtt ggtaagcaga 780 gtctaaatattagacttgct aaggctagag cgtcgcttga tgggttcatt gattcgatta 840 ttgatgaccatattattaga aagaaagctt atgttaatgg caaaaatgat ggaggtgatc 900 gagaaactgatatggtggat gagcttttag ctttttacag tgaggaagca aaagtaactg 960 agtccgaagatttgcagaat gctatcagac ttactaagga tagtatcaaa gctatcatca 1020 tggatgtaatgtttggaggg acagaaacag tggcttctgc aatagaatgg gccatggcag 1080 agcttatgaggagtcctgaa gatcttaaaa aagtacaaca agggctggct aacgttgttg 1140 gactcaacagaaaagttgaa gaatctgact ttgaaaaatt aacatactta agatgttgtc 1200 taaaagaaactctacgactt caccctccaa tccctctcct cctccatgag accgccgagg 1260 aatccaccgtctccggctac catattccgg caaagtcaca tgttattata aattcatttg 1320 ccattgggcgtgacaaaaat tcatgggaag atcctgaaac ttataaacca tctaggtttc 1380 tcaaagaaggtgtaccagat tttaaaggag gtaattttga gtttatacca tttgggtcgg 1440 gtcggcggtcttgccccggt atgcaacttg ggctttatgc attggaaatg gctgtggccc 1500 atcttcttcattgttttact tgggaattgc cagatggtat gaaaccaagt gagcttaaaa 1560 tggatgatatttttggactc actgctccaa gagctaatcg actcgtggct gtgcctactc 1620 cacgcttgttgtgtcccctt tattaattga agaaaaaagg tggggct 1667 188 525 PRTNICOTIANATABACUM 188 Met Lys Glu Met Val Gln Asn Asn Met Ser Thr Ser LeuLeu Glu Thr 1 5 10 15 Leu Gln Ala Thr Pro Met Ile Phe Tyr Phe Ile ValPro Leu Phe Cys 20 25 30 Leu Phe Leu Leu Ser Lys Ser Arg Arg Lys Arg LeuPro Pro Gly Pro 35 40 45 Thr Gly Trp Pro Leu Ile Gly Asn Met Met Met MetAsp Gln Leu Thr 50 55 60 His Arg Gly Leu Ala Lys Leu Ala Gln Lys Tyr GlyGly Val Phe His 65 70 75 80 Leu Lys Met Gly Tyr Val His Lys Ile Val ValSer Gly Pro Asp Glu 85 90 95 Ala Arg Gln Val Leu Gln Glu His Asp Ile IlePhe Ser Asn Arg Pro 100 105 110 Ala Thr Val Ala Ile Ser Tyr Leu Thr TyrAsp Arg Ala Asp Met Ala 115 120 125 Phe Ala Asp Tyr Gly Leu Phe Trp ArgGln Met Arg Lys Leu Cys Val 130 135 140 Met Lys Leu Phe Ser Arg Lys ArgAla Glu Ser Trp Asp Ser Val Arg 145 150 155 160 Asp Glu Ala Asp Ser MetVal Arg Ile Val Thr Thr Asn Thr Gly Thr 165 170 175 Ala Val Asn Leu GlyGlu Leu Val Phe Ser Leu Thr Arg Asn Ile Ile 180 185 190 Tyr Arg Ala AlaPhe Gly Thr Cys Ser Glu Asp Gly Gln Gly Glu Phe 195 200 205 Ile Glu IleMet Gln Glu Phe Ser Lys Leu Phe Gly Ala Phe Asn Ile 210 215 220 Ala AspPhe Ile Pro Trp Leu Gly Trp Val Gly Lys Gln Ser Leu Asn 225 230 235 240Ile Arg Leu Ala Lys Ala Arg Ala Ser Leu Asp Gly Phe Ile Asp Ser 245 250255 Ile Ile Asp Asp His Ile Ile Arg Lys Lys Ala Tyr Val Asn Gly Lys 260265 270 Asn Asp Gly Gly Asp Arg Glu Thr Asp Met Val Asp Glu Leu Leu Ala275 280 285 Phe Tyr Ser Glu Glu Ala Lys Val Thr Glu Ser Glu Asp Leu GlnAsn 290 295 300 Ala Ile Arg Leu Thr Lys Asp Ser Ile Lys Ala Ile Ile MetAsp Val 305 310 315 320 Met Phe Gly Gly Thr Glu Thr Val Ala Ser Ala IleGlu Trp Ala Met 325 330 335 Ala Glu Leu Met Arg Ser Pro Glu Asp Leu LysLys Val Gln Gln Gly 340 345 350 Leu Ala Asn Val Val Gly Leu Asn Arg LysVal Glu Glu Ser Asp Phe 355 360 365 Glu Lys Leu Thr Tyr Leu Arg Cys CysLeu Lys Glu Thr Leu Arg Leu 370 375 380 His Pro Pro Ile Pro Leu Leu LeuHis Glu Thr Ala Glu Glu Ser Thr 385 390 395 400 Val Ser Gly Tyr His IlePro Ala Lys Ser His Val Ile Ile Asn Ser 405 410 415 Phe Ala Ile Gly ArgAsp Lys Asn Ser Trp Glu Asp Pro Glu Thr Tyr 420 425 430 Lys Pro Ser ArgPhe Leu Lys Glu Gly Val Pro Asp Phe Lys Gly Gly 435 440 445 Asn Phe GluPhe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly 450 455 460 Met GlnLeu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 465 470 475 480His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 485 490495 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Arg Ala Asn Arg Leu 500505 510 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro Leu Tyr 515 520 525189 1620 DNA NICOTIANATABACUM 189 gggggataag aatatggaga taccatattacagcttaaaa cttacaattt tttcatttgc 60 aattatcttt gtactaagat gggcatggaaaatcttgaat tatgtgtggt taaaaccaaa 120 agaattggag aaatgcatca gacagcagggtttcaaagga aactcttaca aattcttgtt 180 tggggatatg aaagagataa agaaaatgggtgaagaagct atgtctaagc caatcaattt 240 ctctcatgac atgatttggc ctagagtcatgcccttcatc cacaaaacca tcaccaatta 300 tggtaagaat tgttttgtgt ggtttgggccaagaccagca gtcctgatca cagacccgga 360 acttgtaaag gaggtgctaa cgaagaatttcgtttatcag aagccacctg gcactccact 420 cacaaaattg gcagcaactg gaattgcaggctatgaaaca gataaatggg ctacacatag 480 aaggcttctc aatcctgctt ttcaccttgacaagttgaag catatgctac ctgcattcca 540 atttactgct tgtgagatgt tgagcaaattggagaaagtt gtctcaccaa atggaacaga 600 gatagatgtg tggccatatc tacaaactttaacaagtgat gccatttcaa gaactgcttt 660 tggcagtagt tatgaagaag gaagaaagctttttgaactt caaaaggaac aactttcact 720 aattctagaa gtgtcccgca caatatacatcccaggatgg aggtttttgc caacaaaaag 780 gaacaaaagg atgaagcaaa tatttaatgaagtacgagcg ctggtattgg gaattattaa 840 gaaaagattg agtatgattg aaaatggagaagctcctgat gatttattgg gtatattatt 900 ggcatccaat ttaaaagaaa tccaacaacatggaaataac aagaaatttg gtatgagtat 960 tgatgaggtg attgaagagt gtaaactcttctattttgcg gggcaagaga caacttcatc 1020 tttacttgta tggactatga ttttgttgtgcaaacatcct agttggcaag ataaagctag 1080 agaagaggtt ttgcaagtgt ttggaagtagggaagttgac tatgacaagt tgaatcagct 1140 aaaaatagta actatgatct taaacgaggtcttaaggttg tatccagcag gatatgcgat 1200 taatcgaatg gtaaccaaag aaacaaagttagggaattta tgtttaccag ctggggtaca 1260 actcttgtta ccaacaattt tgttgcaacatgatactgaa atatggggag atgatgcaat 1320 ggagttcaat ccagagagat ttagtgatggaatatccaaa gcaacaaaag gaaaacttgt 1380 gttctttcca tttagttggg gtccaagaatatgtattggg caaaattttg ctatgttaga 1440 ggccaagatg gcaatggcta tgattctgaaaaactatgca tttgaactct ctccatctta 1500 tgctcatgct cctcatccac tactacttcaacctcaatat ggtgctcaat taattttgta 1560 caagttgtag aaatggtcaa tttggaacttgttatggaac ttttatcatc gtaatcaacc 1620 190 518 PRT NICOTIANATABACUM 190Met Glu Ile Pro Tyr Tyr Ser Leu Lys Leu Thr Ile Phe Ser Phe Ala 1 5 1015 Ile Ile Phe Val Leu Arg Trp Ala Trp Lys Ile Leu Asn Tyr Val Trp 20 2530 Leu Lys Pro Lys Glu Leu Glu Lys Cys Ile Arg Gln Gln Gly Phe Lys 35 4045 Gly Asn Ser Tyr Lys Phe Leu Phe Gly Asp Met Lys Glu Ile Lys Lys 50 5560 Met Gly Glu Glu Ala Met Ser Lys Pro Ile Asn Phe Ser His Asp Met 65 7075 80 Ile Trp Pro Arg Val Met Pro Phe Ile His Lys Thr Ile Thr Asn Tyr 8590 95 Gly Lys Asn Cys Phe Val Trp Phe Gly Pro Arg Pro Ala Val Leu Ile100 105 110 Thr Asp Pro Glu Leu Val Lys Glu Val Leu Thr Lys Asn Phe ValTyr 115 120 125 Gln Lys Pro Pro Gly Thr Pro Leu Thr Lys Leu Ala Ala ThrGly Ile 130 135 140 Ala Gly Tyr Glu Thr Asp Lys Trp Ala Thr His Arg ArgLeu Leu Asn 145 150 155 160 Pro Ala Phe His Leu Asp Lys Leu Lys His MetLeu Pro Ala Phe Gln 165 170 175 Phe Thr Ala Cys Glu Met Leu Ser Lys LeuGlu Lys Val Val Ser Pro 180 185 190 Asn Gly Thr Glu Ile Asp Val Trp ProTyr Leu Gln Thr Leu Thr Ser 195 200 205 Asp Ala Ile Ser Arg Thr Ala PheGly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Lys Leu Phe Glu Leu Gln LysGlu Gln Leu Ser Leu Ile Leu Glu Val 225 230 235 240 Ser Arg Thr Ile TyrIle Pro Gly Trp Arg Phe Leu Pro Thr Lys Arg 245 250 255 Asn Lys Arg MetLys Gln Ile Phe Asn Glu Val Arg Ala Leu Val Leu 260 265 270 Gly Ile IleLys Lys Arg Leu Ser Met Ile Glu Asn Gly Glu Ala Pro 275 280 285 Asp AspLeu Leu Gly Ile Leu Leu Ala Ser Asn Leu Lys Glu Ile Gln 290 295 300 GlnHis Gly Asn Asn Lys Lys Phe Gly Met Ser Ile Asp Glu Val Ile 305 310 315320 Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr Ser Ser 325330 335 Leu Leu Val Trp Thr Met Ile Leu Leu Cys Lys His Pro Ser Trp Gln340 345 350 Asp Lys Ala Arg Glu Glu Val Leu Gln Val Phe Gly Ser Arg GluVal 355 360 365 Asp Tyr Asp Lys Leu Asn Gln Leu Lys Ile Val Thr Met IleLeu Asn 370 375 380 Glu Val Leu Arg Leu Tyr Pro Ala Gly Tyr Ala Ile AsnArg Met Val 385 390 395 400 Thr Lys Glu Thr Lys Leu Gly Asn Leu Cys LeuPro Ala Gly Val Gln 405 410 415 Leu Leu Leu Pro Thr Ile Leu Leu Gln HisAsp Thr Glu Ile Trp Gly 420 425 430 Asp Asp Ala Met Glu Phe Asn Pro GluArg Phe Ser Asp Gly Ile Ser 435 440 445 Lys Ala Thr Lys Gly Lys Leu ValPhe Phe Pro Phe Ser Trp Gly Pro 450 455 460 Arg Ile Cys Ile Gly Gln AsnPhe Ala Met Leu Glu Ala Lys Met Ala 465 470 475 480 Met Ala Met Ile LeuLys Asn Tyr Ala Phe Glu Leu Ser Pro Ser Tyr 485 490 495 Ala His Ala ProHis Pro Leu Leu Leu Gln Pro Gln Tyr Gly Ala Gln 500 505 510 Leu Ile LeuTyr Lys Leu 515 191 1566 DNA NICOTIANATABACUM 191 gtcctaacta aaaatggagattcagttttc taacttagtt gcattcttgc tctttctctc 60 cagcatcttt cttctattcaaaaaatggaa aaccagaaaa ctaaatttgc ctcctggtcc 120 atggaaatta ccttttattggaagtttaca ccatttggct gtggcaggtc cacttcctca 180 ccatggccta aaaaatttagccaaacgcta tggtcctctt atgcatttac aacttggaca 240 aattcctaca ctcatcatatcatcacctca aatggcaaaa gaagtactaa aaactcacga 300 cctcgctttt gccactagaccaaagcttgt cgtggccgac atcattcact acgacagcac 360 ggacatagca ttttctccgtacggtgaata ctggagacaa attcgtaaaa tttgcatatt 420 ggaactcttg agtgccaagatggtcaaatt ttttagctcg attcgccaag atgagctctc 480 gaagatgctc tcatctatacgaacgacacc caatcttaca gtcaatctta ctgacaaaat 540 tttttggttt acgagttcggtaacttgtag atcagcttta gggaagatat gtggtgacca 600 agacaaattg atcatttttatgagggaaat aatatcattg gcaggtggat ttagtattgc 660 tgattttttc cctacatggaaaatgattca tgatattgat ggttcgaaat ctaaactggt 720 gaaagcacat cgtaagattgatgaaatttt gggaaatgtt gttgatgagc acaaaaagaa 780 cagagcagat ggcaagaagggtaatggtga atttggtggt gaagatttga ttgatgtatt 840 gttaagagtt agagaaagtggagaagttca aattcctatc acaaatgaca atatcaaatc 900 aatattaatc gacatgttctctgcgggatc tgaaacatca tcgacgacta taatttgggc 960 attagctgaa atgatgaagaaaccaagtgt tttagcaaag gcacaagctg aagtaaggca 1020 agctttgaag gagaaaaaaggttttcaaca gattgatctt gatgagctaa aatatctcaa 1080 gttagtaatc aaagaaaccttaagaatgca ccctccaatt cctctattag ttcctagaga 1140 atgtatggag gatacaaagattgatggtta caatatacct ttcaaaacaa gagtcatagt 1200 taatgcatgg gcaatcggacgagatccaga aagttgggat gaccccgaaa gctttatgcc 1260 agagagattt gagaatagttctattgactt tcttggaaat catcatcagt ttataccatt 1320 tggtgcagga agaaggatttgtccgggaat gctatttggt ttagctaatg ttggacaacc 1380 tttagctcag ttactttatcacttcgattg gaaactccct aatggacaaa gtcatgagaa 1440 tttcgacatg actgagtcacctggaatttc tgctacaaga aaggatgatc ttgttttgat 1500 tgccactcct tatgattcttattaagcagt agcagaaata aaaagccggg gcaaacagaa 1560 aaaagt 1566 192 503 PRTNICOTIANATABACUM 192 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu LeuPhe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu Phe Lys Lys Trp Lys Thr ArgLys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly SerLeu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu LysAsn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly GlnIle Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser Pro Gln Met Ala Lys Glu ValLeu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val ValAla Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser ProTyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu GluLeu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg GlnAsp Glu Leu Ser Lys Met Leu Ser 145 150 155 160 Ser Ile Arg Thr Thr ProAsn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr SerSer Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp GlnAsp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala GlyGly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile HisAsp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp Glu His Lys Lys Asn 245 250255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe SerAla 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu AlaGlu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln AlaGlu Val Arg Gln 325 330 335 Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln IleAsp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu Lys Leu Val Ile Lys Glu ThrLeu Arg Met His Pro Pro 355 360 365 Ile Pro Leu Leu Val Pro Arg Glu CysMet Glu Asp Thr Lys Ile Asp 370 375 380 Gly Tyr Asn Ile Pro Phe Lys ThrArg Val Ile Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro GluSer Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410 415 Glu Arg Phe Glu AsnSer Ser Ile Asp Phe Leu Gly Asn His His Gln 420 425 430 Phe Ile Pro PheGly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe 435 440 445 Gly Leu AlaAsn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His Phe 450 455 460 Asp TrpLys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met Thr 465 470 475 480Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu Ile 485 490495 Ala Thr Pro Tyr Asp Ser Tyr 500 193 1652 DNA NICOTIANATABACUM 193caactaacaa acacattgag tcctctccca aatcactgat tcaccaccaa aagtaccaac 60aattcaatgg aaggtacaaa cttgactaca tatgcagcag tatttcttga tactctgttt 120cttttgttcc tttccaaact tcttcgccag aggaaactca atttacctcc aggcccaaaa 180ccatggccga tcatcggaaa cttaaacctt attggcaatc ttcctcatcg ctcaatccac 240gaactctccc tcaagtacgg acccgttatg caactccaat tcgggtcttt ccccgttgta 300gttggatcct ccgtcgaaat ggctaagatt ttcctcaaat ccatggatat taactttgta 360ggcaggccta aaacggctgc cggaaaatac acaacgtaca attattccga tattacatgg 420tctccttacg gaccatattg gcgccaggca cgtaggatgt gcctaacgga attattcagc 480acgaaacgtc tcgattcata cgagtatatt cgggctgagg agttgcattc tcttctccat 540aatttgaaca aaatatcagg gaaaccaatt gtgttgaaag attattcgac gacgttgagt 600ttaaatgtta ttagcaggat ggtactgggg aaaaggtatt tggacgaatc cgagaactcg 660ttcgtgaatc ctgaggaatt taagaagatg ttggacgaat tgtttttgct aaatggtgta 720cttaatattg gagattcaat tccatggatt gatttcatgg atttgcaagg ttatgttaag 780aggatgaaag tagtgagcaa gaaattcgac aagtttttag agcatgttat tgatgagcat 840aacattagga gaaatggagt ggagaattat gttgctaagg atatggtgga tgttttgttg 900cagctcgctg atgatccgaa gttggaagtt aagctggaga gacatggagt caaagcattc 960actcaggata tgctggctgg tggaaccgag agttcagcag tgacagtgga gtgggcaatt 1020tcagagctgc taaagaagcc ggagattttc aaaaaggcta cagaagaatt ggatcgagta 1080attgggcaga atagatgggt acaagaaaag gacattccaa atcttcctta catagaggca 1140atagtcaaag agactatgcg actgcacccc gtggcaccaa tgttggtgcc acgtgagtgt 1200cgagaagata ttaaggtagc aggctacgac gttcagaaag gaactagggt tctcgtgagt 1260gtatggacta ttggaagaga ccctacattg tgggacgagc ctgaggtgtt caagccggag 1320agattccatg aaaggtccat agatgttaaa ggacatgatt atgagctttt gccatttgga 1380gcggggagaa gaatgtgccc gggttatagc ttggggctca aggtgattca agctagctta 1440gctaatcttc tacatggatt taactggtca ttgcctgata atatgactcc tgaggacctc 1500aacatggatg agatttttgg gctctctaca cctaaaaaat ttccacttgc tactgtgatt 1560gagccaagac tttcaccaaa actttactct gtttgattca gcagttctat ggttccgtca 1620agatagactt tgttacgttt gaacctgtgc tc 1652 194 509 PRT NICOTIANATABACUM194 Met Glu Gly Thr Asn Leu Thr Thr Tyr Ala Ala Val Phe Leu Asp Thr 1 510 15 Leu Phe Leu Leu Phe Leu Ser Lys Leu Leu Arg Gln Arg Lys Leu Asn 2025 30 Leu Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 3540 45 Ile Gly Asn Leu Pro His Arg Ser Ile His Glu Leu Ser Leu Lys Tyr 5055 60 Gly Pro Val Met Gln Leu Gln Phe Gly Ser Phe Pro Val Val Val Gly 6570 75 80 Ser Ser Val Glu Met Ala Lys Ile Phe Leu Lys Ser Met Asp Ile Asn85 90 95 Phe Val Gly Arg Pro Lys Thr Ala Ala Gly Lys Tyr Thr Thr Tyr Asn100 105 110 Tyr Ser Asp Ile Thr Trp Ser Pro Tyr Gly Pro Tyr Trp Arg GlnAla 115 120 125 Arg Arg Met Cys Leu Thr Glu Leu Phe Ser Thr Lys Arg LeuAsp Ser 130 135 140 Tyr Glu Tyr Ile Arg Ala Glu Glu Leu His Ser Leu LeuHis Asn Leu 145 150 155 160 Asn Lys Ile Ser Gly Lys Pro Ile Val Leu LysAsp Tyr Ser Thr Thr 165 170 175 Leu Ser Leu Asn Val Ile Ser Arg Met ValLeu Gly Lys Arg Tyr Leu 180 185 190 Asp Glu Ser Glu Asn Ser Phe Val AsnPro Glu Glu Phe Lys Lys Met 195 200 205 Leu Asp Glu Leu Phe Leu Leu AsnGly Val Leu Asn Ile Gly Asp Ser 210 215 220 Ile Pro Trp Ile Asp Phe MetAsp Leu Gln Gly Tyr Val Lys Arg Met 225 230 235 240 Lys Val Val Ser LysLys Phe Asp Lys Phe Leu Glu His Val Ile Asp 245 250 255 Glu His Asn IleArg Arg Asn Gly Val Glu Asn Tyr Val Ala Lys Asp 260 265 270 Met Val AspVal Leu Leu Gln Leu Ala Asp Asp Pro Lys Leu Glu Val 275 280 285 Lys LeuGlu Arg His Gly Val Lys Ala Phe Thr Gln Asp Met Leu Ala 290 295 300 GlyGly Thr Glu Ser Ser Ala Val Thr Val Glu Trp Ala Ile Ser Glu 305 310 315320 Leu Leu Lys Lys Pro Glu Ile Phe Lys Lys Ala Thr Glu Glu Leu Asp 325330 335 Arg Val Ile Gly Gln Asn Arg Trp Val Gln Glu Lys Asp Ile Pro Asn340 345 350 Leu Pro Tyr Ile Glu Ala Ile Val Lys Glu Thr Met Arg Leu HisPro 355 360 365 Val Ala Pro Met Leu Val Pro Arg Glu Cys Arg Glu Asp IleLys Val 370 375 380 Ala Gly Tyr Asp Val Gln Lys Gly Thr Arg Val Leu ValSer Val Trp 385 390 395 400 Thr Ile Gly Arg Asp Pro Thr Leu Trp Asp GluPro Glu Val Phe Lys 405 410 415 Pro Glu Arg Phe His Glu Arg Ser Ile AspVal Lys Gly His Asp Tyr 420 425 430 Glu Leu Leu Pro Phe Gly Ala Gly ArgArg Met Cys Pro Gly Tyr Ser 435 440 445 Leu Gly Leu Lys Val Ile Gln AlaSer Leu Ala Asn Leu Leu His Gly 450 455 460 Phe Asn Trp Ser Leu Pro AspAsn Met Thr Pro Glu Asp Leu Asn Met 465 470 475 480 Asp Glu Ile Phe GlyLeu Ser Thr Pro Lys Lys Phe Pro Leu Ala Thr 485 490 495 Val Ile Glu ProArg Leu Ser Pro Lys Leu Tyr Ser Val 500 505 195 1657 DNANICOTIANATABACUM 195 cttcttcctt cctaactaaa aatggagatt cagttttctaacttagttgc attcttgctc 60 tttctctcca gcatctttct tgtattcaaa aaatggaaaaccagaaaact aaatttgcct 120 cctggtccat ggaaattacc ttttattgga agtttacaccatttggctgt ggcaggtcca 180 cttcctcacc atggcctaaa aaatttagcc aaacgctatggtcctcttat gcatttacaa 240 cttggacaaa ttcctacact cgtcatatca tcacctcaaatggcaaaaga agtactaaaa 300 actcacgacc tcgcttttgc cactagacca aagcttgtcgtggccgacat cattcactac 360 gacagcacgg acatagcatt ttcgccatac ggtgaatactggagacaaat tcgtaaaatt 420 tgcatattgg aactcttgag tgccaagatg gtcaagttttttagctcgat tcgccaagat 480 gagctctcga agatggtttc atctatacga acgacgcccaatcttccagt caatcttacc 540 gacaagattt tttggtttac gagttcggta atttgtagatcagctttagg gaagatatgt 600 ggtgaccaag acaaattgat catttttatg agggaaataatatcattggc aggtggattt 660 agtattgctg attttttccc tacatggaaa atgattcatgatattgatgg ttcaaaatct 720 aaactggtga aggcacatcg taagattgat gaaattttggaaaatgtggt aaatgagcac 780 aaacagaatc gagcagatgg taaaaagggt aatggtgaatttggtggaga agatctgatt 840 gatgttttgt taagagttag agaaagtgga gaagttcaaattccaatcac agatgacaat 900 atcaaatcaa tattaatcga catgttctct gccggatcggaaacatcatc gacaactata 960 atttgggcat tagctgaaat gatgaagaaa ccaagtgttttagcaaaggc acaagctgaa 1020 gtgaggcaag ctttgaaggg gaagaaaatt agttttcaagagattgatat tgataagcta 1080 aagtatttga agttagtgat caaagaaact ttaagaatgcaccctccaat tcctctgtta 1140 gtccctagag aatgtatgga agatacaaag attgatggttacaatatacc tttcaaaaca 1200 agagtcattg ttaatgcatg ggcaattgga cgagatcctcaaagttggga tgatcctgaa 1260 agctttacgc cagagagatt tgagaataat tctattgattttcttggaaa tcatcatcaa 1320 tttattccat ttggtgcagg aagaaggatt tgtcctggaatgctatttgg tttagctaat 1380 gttggacaac ctttagctca gttactttat cacttcgattggaaactccc taatggacaa 1440 agtcatgaga atttcgacat gactgagtca cctggaatttctgctacaag aaaggatgat 1500 cttgttttga ttgccactcc ttatgattct tattaagcagtagcagaaat aaaaagccgg 1560 ggcaaacaga aaaaagtatt gctgcttcta ggtattttctattggataaa tttcaaaatt 1620 catccacaat atttagtgtt tgctagagtt ggttagc 1657196 504 PRT NICOTIANATABACUM 196 Met Glu Ile Gln Phe Ser Asn Leu Val AlaPhe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Val Phe Lys Lys TrpLys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro PheIle Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His HisGly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu GlnLeu Gly Gln Ile Pro Thr Leu 65 70 75 80 Val Ile Ser Ser Pro Gln Met AlaLys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro LysLeu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile AlaPhe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile CysIle Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser SerIle Arg Gln Asp Glu Leu Ser Lys Met Val Ser 145 150 155 160 Ser Ile ArgThr Thr Pro Asn Leu Pro Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe TrpPhe Thr Ser Ser Val Ile Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 CysGly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225230 235 240 Lys Ile Asp Glu Ile Leu Glu Asn Val Val Asn Glu His Lys GlnAsn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly GluAsp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu ValGln Ile Pro 275 280 285 Ile Thr Asp Asp Asn Ile Lys Ser Ile Leu Ile AspMet Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile TrpAla Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala LysAla Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Gly Lys Lys Ile SerPhe Gln Glu Ile Asp Ile Asp Lys 340 345 350 Leu Lys Tyr Leu Lys Leu ValIle Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Ile Pro Leu Leu ValPro Arg Glu Cys Met Glu Asp Thr Lys Ile 370 375 380 Asp Gly Tyr Asn IlePro Phe Lys Thr Arg Val Ile Val Asn Ala Trp 385 390 395 400 Ala Ile GlyArg Asp Pro Gln Ser Trp Asp Asp Pro Glu Ser Phe Thr 405 410 415 Pro GluArg Phe Glu Asn Asn Ser Ile Asp Phe Leu Gly Asn His His 420 425 430 GlnPhe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu 435 440 445Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His 450 455460 Phe Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met 465470 475 480 Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu ValLeu 485 490 495 Ile Ala Thr Pro Tyr Asp Ser Tyr 500 197 1610 DNANICOTIANATABACUM 197 cttcttcctt cctaactaaa aatggagatt cagttttctaacttagttgc attcttgctc 60 tttctctcca gcatctttct tgtattcaaa aaatggaaaaccagaaaact aaatttgcct 120 cctggtccat ggaaattacc ttttattgga agtttacaccatttggctgt ggcaggtcca 180 cttcctcacc atggcctaaa aaatttagcc aaacgctatggtcctcttat gcatttacaa 240 cttggacaaa ttcctacact cgtcatatca tcacctcaaatggcaaaaga agtactaaaa 300 actcacgacc tcgcttttgc cactagacca aagcttgtcgtggccgacat cattcactac 360 gacagcacgg acatagcact ttcgccatac ggtgaatactggagacaaat tcgtaaaatt 420 tgcatattgg aactcttgag tgccaagatg gtcaagttttttagctcgat tcgccaagat 480 gagctctcga agatggtttc atctatacga acgacgcccaatcttccagt caatcttacc 540 gacaagattt tttggtttac gagttcggta atttgtagatcagctttagg gaagatatgt 600 ggtgaccaag acaaattgat catttttatg agggaaataatatcattggc aggtggattt 660 agtattgctg attttttccc tacatggaaa atgattcatgatattgatgg ttcaaaatct 720 aaactggtga aggcacatcg taagattgat gaaattttggaaaatgtggt aaatgagcac 780 aaacagaatc gagcagatgg taaaaagggt aatggtgaatttggtggaga agatctgatt 840 gatgttttgt taagagttag agaaagtgga gaagttcaaattccaatcac agatgacaat 900 atcaaatcaa tattaatcga catgttctct gccggatcggaaacatcatc gacaactata 960 atttgggcat tagctgaaat gatgaagaaa ccaagtgttttagcaaaggc acaagctgaa 1020 gtgagccaag ctttgaaggg gaagaaaatt agttttcaagagattgatat tgataagcta 1080 aagtatttga agttagtgat caaagaaact ttaagaatgcaccctccaat tcctctgtta 1140 gtccctagag aatgtatgga agatacaaag attgatggttacaatatacc tttcaaaaca 1200 agagtcattg ttaatgcatg ggcaattgga cgagatcctcaaagttggga tgatcctgaa 1260 agctttacgc cagagagatt tgagaataat tctattgattttcttggaaa tcatcatcaa 1320 tttattccat ttggtgcagg aagaaggatt tgtcctggaatgctatttgg tttagctaat 1380 gttggacaac ctttagctca gttactttat cacttcgattggaaactccc taatggacaa 1440 actcaccaaa atttcgacat gactgagtca cctggaatttctgctacaag aaaggatgat 1500 cttattttga ttgccactcc tgctcattct tgattaagtattgctgcttt tctattggag 1560 aattttcaaa attcatccac aatatatagt gtttgctagagttggttagc 1610 198 503 PRT NICOTIANATABACUM 198 Met Glu Ile Gln Phe SerAsn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu ValPhe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro TrpLys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly ProLeu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro LeuMet His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Val Ile Ser SerPro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe AlaThr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp SerThr Asp Ile Ala Leu Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln IleArg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 LysPhe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Val Ser 145 150 155160 Ser Ile Arg Thr Thr Pro Asn Leu Pro Val Asn Leu Thr Asp Lys Ile 165170 175 Phe Trp Phe Thr Ser Ser Val Ile Cys Arg Ser Ala Leu Gly Lys Ile180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile IleSer 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr TrpLys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val LysAla His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Glu Asn Val Val AsnGlu His Lys Gln Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly GluPhe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg GluSer Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asp Asp Asn Ile Lys SerIle Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser ThrThr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro SerVal Leu Ala Lys Ala Gln Ala Glu Val Ser Gln 325 330 335 Ala Leu Lys GlyLys Lys Ile Ser Phe Gln Glu Ile Asp Ile Asp Lys 340 345 350 Leu Lys TyrLeu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro IlePro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile 370 375 380 AspGly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp 385 390 395400 Ala Ile Gly Arg Asp Pro Gln Ser Trp Asp Asp Pro Glu Ser Phe Thr 405410 415 Pro Glu Arg Phe Glu Asn Asn Ser Ile Asp Phe Leu Gly Asn His His420 425 430 Gln Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly MetLeu 435 440 445 Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu LeuTyr His 450 455 460 Phe Asp Trp Lys Leu Pro Asn Gly Gln Thr His Gln AsnPhe Asp Met 465 470 475 480 Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg LysAsp Asp Leu Ile Leu 485 490 495 Ile Ala Thr Pro Ala His Ser 500 199 1664DNA NICOTIANATABACUM 199 atccttcttc cttcctaggt cctaactaaa aatggagattcagttttcta acttagttgc 60 attcttgctc tttctctcca gcatctttct tctattcaaaaaatggaaaa ccagaaaact 120 aaatttgcct cctggtccat ggaaattacc ttttattggaagtttacacc atttggctgt 180 ggcaggtcca cttcctcacc atggcctaaa aaatttagccaaacgctatg gtcctcttat 240 gcatttacaa cttggacaaa ttcctacact catcatatcatcacctcaaa tggcaaaaga 300 agtactaaaa actcacgacc tcgcttttgc cactagaccaaagcttgtcg tggccgacat 360 cattcactac gacagcacgg acatagcatt ttctccgtacggtgaatact ggagacaaat 420 tcgtaaaatt tgcatattgg aactcttgag tgccaagatggtcaaatttt ttagctcgat 480 tcgccaagat gagctctcga agatgctctc atctatacgaacgacaccca atcttacagt 540 caatcttact gacaaaattt tttggtttac gagttcggtaacttgtagat cagctttagg 600 gaagatatgt ggtgaccaag acaaattgat catttttatgagggaaataa tatcattggc 660 aggtggattt agtattgctg attttttccc tacatggaaaatgattcatg atattgatgg 720 ttcgaaatct aaactggtga aagcacatcg taagattgatgaaattttgg gaaatgttgt 780 tgatgagcac aaaaagaaca gagcagatgg caagaagggtaatggtgaat ttggtggtga 840 agatttgatt gatgtattgt taagagttag agaaagtggagaagttcaaa ttcctatcac 900 aaatgacaat atcaaatcaa tattaatcga catgttctctgcgggatctg aaacatcatc 960 gacgactata atttgggcat tagctgaaat gatgaagaaaccaagtgttt tagcaaaggc 1020 acaagctgaa gtaaggcaag ctttgaagga gaaaaaaggttttcaacaga ttgatcttga 1080 tgagctaaaa tatctcaagt tagtaatcaa agaaaccttaagaatgcacc ctccaattcc 1140 tctattagtt cctagagaat gtatggagga tacaaagattgatggttaca atataccttt 1200 caaaacaaga gtcatagtta atgcatgggc aatcggacgagatccagaaa gttgggatga 1260 ccccgaaagc tttatgccag agagatttga gaatagttctattgactttc ttggaaatca 1320 tcatcagttt ataccatttg gtgcaggaag aaggatttgtccgggaatgc tatttggttt 1380 agctaatgtt ggacaacctt tagctcagtt actttatcacttcgattgga aactccctaa 1440 tggacaaagt catgagaatt tcgacatgac tgagtcacctggaatttctg ctacaagaaa 1500 ggatgatctt gttttgattg ccactcctta tgattcttattaagcagtag cagaaataaa 1560 aagccggggc aaacagaaaa aagtattgct gcttctaggtattttctatt ggataaattt 1620 caaaattcat ccacaatatt tagtgtttgc tagagttggttagc 1664 200 503 PRT NICOTIANATABACUM 200 Met Glu Ile Gln Phe Ser AsnLeu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu PheLys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp LysLeu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro LeuPro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu MetHis Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser ProGln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala ThrArg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser ThrAsp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile ArgLys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys PhePhe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Leu Ser 145 150 155 160Ser Ile Arg Thr Thr Pro Asn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170175 Phe Trp Phe Thr Ser Ser Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp LysMet 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys AlaHis Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp GluHis Lys Lys Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu PheGly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu SerGly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser IleLeu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr ThrIle Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser ValLeu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Glu LysLys Gly Phe Gln Gln Ile Asp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu LysLeu Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Ile Pro LeuLeu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile Asp 370 375 380 Gly TyrAsn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp Ala 385 390 395 400Ile Gly Arg Asp Pro Glu Ser Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410415 Glu Arg Phe Glu Asn Ser Ser Ile Asp Phe Leu Gly Asn His His Gln 420425 430 Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe435 440 445 Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr HisPhe 450 455 460 Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe AspMet Thr 465 470 475 480 Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp AspLeu Val Leu Ile 485 490 495 Ala Thr Pro Tyr Asp Ser Tyr 500 201 1625 DNANICOTIANATABACUM 201 ttcttatttt gattcaacca tggagaacca atactcctactcattctctt cctacttcta 60 cttagctata gtactgtttc ttcttccaat tttggtcaaatatttcttcc atcggagaag 120 aaatttacct ccaagtccat tttctcttcc aataattggtcacctttacc ttctcaagaa 180 aactctccat ctcactctaa catccttatc agctaaatatggtcctgttt tatacctcaa 240 attgggctct atgcctgtga ttgttgtgtc ctcaccatctgctgttgaag aatgtttaac 300 caagaatgat atcatattcg caaataggcc caagaccgtggctggtgaca agtttaccta 360 caattatact gtttatgttt gggcacccta tggccaactttggagaattc ttcgccgatt 420 aactgtcgtt gaactcttct cttcacatag cctacagaaaacttctatcc ttagagatca 480 agaagttgca atatttatcc gttcgttata caaattctcaaaggatagta gcaaaaaagt 540 cgatttgacc aactggtctt ttactttggt tttcaatcttatgaccaaaa ttattgctgg 600 gagacatatt gtgaaggagg aagatgctgg caaggaaaagggcattgaaa ttattgaaaa 660 acttagaggg actttcttag taactacatc attcttgaatatgtgtgatt tcttgccagt 720 attcaggtgg gttggttaca aagggctgga gaagaagatggcctcaattc acaatagaag 780 aaatgaattc ttgaacagct tgcttgatga atttcgacacaagaaaagta gtgcttcaca 840 atctaacaca actgttggaa acatggagaa gaaaaccacactgattgaaa agctcttgtc 900 tcttcaagaa tcagagcctg aattctacac tgatgatatcatcaaaagta ttatgctggt 960 agtttttgtt gcaggaacag agacctcatc aacaaccatccaatgggtaa tgaggcttct 1020 tgtagctcac cctgaggcat tgtataagct acgagctgacattgacagta aagttgggaa 1080 taagcgcttg ctgaatgaat cagacctcaa caagcttccgtatttgcatt gtgttgttaa 1140 tgagacaatg agattataca ctccgatacc acttttattgcctcattatt caactaaaga 1200 ttgtattgtg gaaggatatg atgtaccaaa acatacaatgttgtttgtca acgcttgggc 1260 cattcacagg gatcccaagg tatgggagga gcctgacaagttcaagccag agagatttga 1320 ggcaacagaa ggggaaacag aaaggttcaa ttacaagcttgtaccatttg gaatggggag 1380 aagagcgtgc cctggagctg atatggggtt gcgagcagtttctttggcat taggtgcact 1440 tattcaatgc tttgactggc aaattgagga agcggaaagcttggaggaaa gctataattc 1500 tagaatgact atgcagaaca agcctttgaa ggttgtctgcactccacgcg aagatcttgg 1560 ccagcttcta tcccaactct aaggcaattt atcaatgccaaacgtaatct tcatctacca 1620 ctatg 1625 202 520 PRT NICOTIANATABACUM 202Met Glu Asn Gln Tyr Ser Tyr Ser Phe Ser Ser Tyr Phe Tyr Leu Ala 1 5 1015 Ile Val Leu Phe Leu Leu Pro Ile Leu Val Lys Tyr Phe Phe His Arg 20 2530 Arg Arg Asn Leu Pro Pro Ser Pro Phe Ser Leu Pro Ile Ile Gly His 35 4045 Leu Tyr Leu Leu Lys Lys Thr Leu His Leu Thr Leu Thr Ser Leu Ser 50 5560 Ala Lys Tyr Gly Pro Val Leu Tyr Leu Lys Leu Gly Ser Met Pro Val 65 7075 80 Ile Val Val Ser Ser Pro Ser Ala Val Glu Glu Cys Leu Thr Lys Asn 8590 95 Asp Ile Ile Phe Ala Asn Arg Pro Lys Thr Val Ala Gly Asp Lys Phe100 105 110 Thr Tyr Asn Tyr Thr Val Tyr Val Trp Ala Pro Tyr Gly Gln LeuTrp 115 120 125 Arg Ile Leu Arg Arg Leu Thr Val Val Glu Leu Phe Ser SerHis Ser 130 135 140 Leu Gln Lys Thr Ser Ile Leu Arg Asp Gln Glu Val AlaIle Phe Ile 145 150 155 160 Arg Ser Leu Tyr Lys Phe Ser Lys Asp Ser SerLys Lys Val Asp Leu 165 170 175 Thr Asn Trp Ser Phe Thr Leu Val Phe AsnLeu Met Thr Lys Ile Ile 180 185 190 Ala Gly Arg His Ile Val Lys Glu GluAsp Ala Gly Lys Glu Lys Gly 195 200 205 Ile Glu Ile Ile Glu Lys Leu ArgGly Thr Phe Leu Val Thr Thr Ser 210 215 220 Phe Leu Asn Met Cys Asp PheLeu Pro Val Phe Arg Trp Val Gly Tyr 225 230 235 240 Lys Gly Leu Glu LysLys Met Ala Ser Ile His Asn Arg Arg Asn Glu 245 250 255 Phe Leu Asn SerLeu Leu Asp Glu Phe Arg His Lys Lys Ser Ser Ala 260 265 270 Ser Gln SerAsn Thr Thr Val Gly Asn Met Glu Lys Lys Thr Thr Leu 275 280 285 Ile GluLys Leu Leu Ser Leu Gln Glu Ser Glu Pro Glu Phe Tyr Thr 290 295 300 AspAsp Ile Ile Lys Ser Ile Met Leu Val Val Phe Val Ala Gly Thr 305 310 315320 Glu Thr Ser Ser Thr Thr Ile Gln Trp Val Met Arg Leu Leu Val Ala 325330 335 His Pro Glu Ala Leu Tyr Lys Leu Arg Ala Asp Ile Asp Ser Lys Val340 345 350 Gly Asn Lys Arg Leu Leu Asn Glu Ser Asp Leu Asn Lys Leu ProTyr 355 360 365 Leu His Cys Val Val Asn Glu Thr Met Arg Leu Tyr Thr ProIle Pro 370 375 380 Leu Leu Leu Pro His Tyr Ser Thr Lys Asp Cys Ile ValGlu Gly Tyr 385 390 395 400 Asp Val Pro Lys His Thr Met Leu Phe Val AsnAla Trp Ala Ile His 405 410 415 Arg Asp Pro Lys Val Trp Glu Glu Pro AspLys Phe Lys Pro Glu Arg 420 425 430 Phe Glu Ala Thr Glu Gly Glu Thr GluArg Phe Asn Tyr Lys Leu Val 435 440 445 Pro Phe Gly Met Gly Arg Arg AlaCys Pro Gly Ala Asp Met Gly Leu 450 455 460 Arg Ala Val Ser Leu Ala LeuGly Ala Leu Ile Gln Cys Phe Asp Trp 465 470 475 480 Gln Ile Glu Glu AlaGlu Ser Leu Glu Glu Ser Tyr Asn Ser Arg Met 485 490 495 Thr Met Gln AsnLys Pro Leu Lys Val Val Cys Thr Pro Arg Glu Asp 500 505 510 Leu Gly GlnLeu Leu Ser Gln Leu 515 520 203 1767 DNA NICOTIANATABACUM 203 aaccaaccttccttttctta cttagtaaaa tggatattca gtcttctcct ttcaacttaa 60 ttgctttgctactcttcatt tcatttcttt ttatcctatt gaaaaagtgg aataccaaaa 120 tcccaaagttacctccaggt ccatggagac ttccccttat tggcagcctc catcacttga 180 aaggtaaactcccacaccat catcttagag atttagcccg aaaatatgga cctctcatgt 240 atttacaacttggagaagtt cctgtagttg taatatcttc gccacgtata gcaaaagctg 300 tactaaaaactcatgatctt gcttttgcaa cgaggcctcg gttcatgtcc tcggacattg 360 tgttttacaaaagcagggac atatcattcg ccccatatgg cgattactgg agacaaatgc 420 gtaaaatattaacacaagaa ctcttgagta acaagatgct caagtcattt agcacaatcc 480 gaaaggatgagctctcgaag ctcctctcgt cgattcgttt agcaacagct tcttctgcag 540 tgaacataaacgaaaagctt ctctggttta caagttgcat gacttgtaga ttagcctttg 600 gaaaaatatgcaacgatcgt gatgaattga ttatgttaat aagggagata ttagcattat 660 caggaggatttgatgtgtgt gatttgttcc cttcatggaa attacttcac aatatgagca 720 acatgaaagctagattgacg aatgttcacc ataagtataa tctaattatg gagaatatca 780 tcaatgagcacaaagagaat catgcagcag ggataaaggg aaataacgag tttggtggcg 840 aagatatgattgatgcttta ctgagggtta aggagaataa tgagcttcaa tttcctatcg 900 aaaatgacaacatgaaagca gtaattctgg acttgtttat tgctggaact gaaacttcat 960 atactgcaattatatgggca ctatcagaat tgatgaagca cccaagtgtt atggccaagg 1020 cacaagctgaagtgagaaaa gtcttcaaag aaaatgaaaa cttggacgaa aatgatcttg 1080 acaagttgccatacttaaaa tcagtgatca aagaaacact aaggatgcat cctccagttc 1140 ctttattaggacctagagaa tgcagagaac aaactgagat tgatggatat actgtacctc 1200 ttaaagctagagtaatggtt aatgcatggg caattggaag agatcctgaa agttgggaag 1260 atcctgaaagtttcaaaccc gagcgatttg aaaatatttc tgttgatctt acgggaaatc 1320 actatcagttcatccctttc ggttcaggaa gaagaatgtg tccaggaatg tcgtttggtt 1380 tagttaacactgggcatcct ttagctcagt tgctctattt ctttgactgg aaattccctc 1440 ataaggttaatgcagctgat tttcacacta ctgaaacaag tagagttttt gcagcaagca 1500 aagatgacctctacttgatt ccaacaaatc acatggagca agagtagctc taaattgaat 1560 tcttgtcttggaacaataaa agaagaaact ccagcttggt ctacattatt tctttttgct 1620 ttatattagtatgggtgtgt tcagtttctt atttttaagg gtaccctgaa agataaaggg 1680 ctatataaaccagtgagact ttttattggt tgcaaggttt tagatcaagc cataagacag 1740 catattttattcaaaaaaaa aaaaaaa 1767 204 505 PRT NICOTIANATABACUM 204 Met Asp Ile GlnSer Ser Pro Phe Asn Leu Ile Ala Leu Leu Leu Phe 1 5 10 15 Ile Ser PheLeu Phe Ile Leu Leu Lys Lys Trp Asn Thr Lys Ile Pro 20 25 30 Lys Leu ProPro Gly Pro Trp Arg Leu Pro Leu Ile Gly Ser Leu His 35 40 45 His Leu LysGly Lys Leu Pro His His His Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr GlyPro Leu Met Tyr Leu Gln Leu Gly Glu Val Pro Val Val 65 70 75 80 Val IleSer Ser Pro Arg Ile Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu AlaPhe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 TyrLys Ser Arg Asp Ile Ser Phe Ala Pro Tyr Gly Asp Tyr Trp Arg 115 120 125Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135140 Lys Ser Phe Ser Thr Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145150 155 160 Ser Ile Arg Leu Ala Thr Ala Ser Ser Ala Val Asn Ile Asn GluLys 165 170 175 Leu Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala PheGly Lys 180 185 190 Ile Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile ArgGlu Ile Leu 195 200 205 Ala Leu Ser Gly Gly Phe Asp Val Cys Asp Leu PhePro Ser Trp Lys 210 215 220 Leu Leu His Asn Met Ser Asn Met Lys Ala ArgLeu Thr Asn Val His 225 230 235 240 His Lys Tyr Asn Leu Ile Met Glu AsnIle Ile Asn Glu His Lys Glu 245 250 255 Asn His Ala Ala Gly Ile Lys GlyAsn Asn Glu Phe Gly Gly Glu Asp 260 265 270 Met Ile Asp Ala Leu Leu ArgVal Lys Glu Asn Asn Glu Leu Gln Phe 275 280 285 Pro Ile Glu Asn Asp AsnMet Lys Ala Val Ile Leu Asp Leu Phe Ile 290 295 300 Ala Gly Thr Glu ThrSer Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu 305 310 315 320 Leu Met LysHis Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg 325 330 335 Lys ValPhe Lys Glu Asn Glu Asn Leu Asp Glu Asn Asp Leu Asp Lys 340 345 350 LeuPro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365Pro Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Glu Gln Thr Glu Ile 370 375380 Asp Gly Tyr Thr Val Pro Leu Lys Ala Arg Val Met Val Asn Ala Trp 385390 395 400 Ala Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser PheLys 405 410 415 Pro Glu Arg Phe Glu Asn Ile Ser Val Asp Leu Thr Gly AsnHis Tyr 420 425 430 Gln Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys ProGly Met Ser 435 440 445 Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala GlnLeu Leu Tyr Phe 450 455 460 Phe Asp Trp Lys Phe Pro His Lys Val Asn AlaAla Asp Phe His Thr 465 470 475 480 Thr Glu Thr Ser Arg Val Phe Ala AlaSer Lys Asp Asp Leu Tyr Leu 485 490 495 Ile Pro Thr Asn His Met Glu GlnGlu 500 505 205 1736 DNA NICOTIANATABACUM 205 aaccaacctt ccttttcttacttagtaaaa tggatattca gtcttctcct ttcaacttaa 60 ttgctttgct actcttcatttcatttcttt ttatcctatt gaaaaagtgg aataccaaaa 120 tcccaaagtt acctccaggtccatggagac ttccccttat tggcagcctc catcacttga 180 aaggtaaact cccacaccatcatcttagag atttagcccg aaaatatgga cctctcatgt 240 atttacaact tggagaagttcctgtagttg taatatcttc gccacgtata gcaaaagctg 300 tactaaaaac tcatgatcttgcttttgcaa cgaggcctcg gttcatgtcc tcggacattg 360 tgttttacaa aagcagggacatatcattcg ccccatatgg cgattactgg agacaaatgc 420 gtaaaatatt aacacaagaactcttgagta acaagatgct caagtcattt agcacaatcc 480 gaaaggatgg gctctcgaagctcctctcgt cgattcgttt agcaacagct tcttctgcag 540 tgaacataaa cgaaaagcttctctggttta caagttgcat gacttgtaga ttagcctttg 600 gaaaaatatg caacgatcgtgatgaattga ttatgttaat aagggagata ttagcattat 660 caggaggatt tgatgtgtgtgatttgttcc cttcatggaa attacttcac aatatgagca 720 acatgaaagc tagattgacgaatgttcacc ataagtataa tctaattatg gagaatatca 780 tcaatgagca caaagagaatcatgcagcag ggataaaggg aaataacgag tttggtggcg 840 aagatatgat tgatgctttactgagggtta aggagaataa tgagcttcaa tttcctatcg 900 aaaatgacaa catgaaagcagtaattctgg acttgtttat tgctggaact gaaacttcat 960 atactgcaat tatatgggcactatcagaat tgatgaagca cccaagtgtt atggccaagg 1020 cacaagctga agtgagaaaagtcttcaaag aaaatgaaaa cttggacgaa aatgatcttg 1080 acaagttgcc atacttaaaatcagtgatca aagaaacact aaggatgcat cctccagttc 1140 ctttattagg acctagagaatgcagagaac aaactgagat tgatggatat actgtacctc 1200 ttaaagctag agtaatggttaatgcatggg caattggaag agatcctgaa agttgggaag 1260 atcctgaaag tttcaaacccgagcgatttg aaaatatttc tgttgatctt acgggaaatc 1320 actatcagtt cattcctttcggttcaggaa gaagaatgtg tccaggaatg tcgtttggtt 1380 tagttaacac tgggcatcctttagctcagt tgctctattt ctttgactgg aaattccctc 1440 ataaggttaa tgcagctgattttcacacta ctgaaacaag tagagttttt gcagcaagca 1500 aagatgacct ctacttgattccaacaaatc acatggagca agagtagctc taaattgaat 1560 tcttgtcttg gaacgataaaagaagaaact ccagcttggt ctacattatt tctttttgct 1620 ttatattagt atgggtgtgttcagtttctt gtttttaagg gtaccctgaa agataaaggg 1680 ctatataaac cagtgagactttttattgaa aaaaaaaaaa aaaaaaaaaa aaaaaa 1736 206 505 PRTNICOTIANATABACUM 206 Met Asp Ile Gln Ser Ser Pro Phe Asn Leu Ile Ala LeuLeu Leu Phe 1 5 10 15 Ile Ser Phe Leu Phe Ile Leu Leu Lys Lys Trp AsnThr Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Leu IleGly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His His Leu ArgAsp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly GluVal Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Ile Ala Lys Ala ValLeu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met SerSer Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala ProTyr Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln GluLeu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Phe Ser Thr Ile Arg LysAsp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Ala ThrAla Ser Ser Ala Val Asn Ile Asn Glu Lys 165 170 175 Leu Leu Trp Phe ThrSer Cys Met Thr Cys Arg Leu Ala Phe Gly Lys 180 185 190 Ile Cys Asn AspArg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu 195 200 205 Ala Leu SerGly Gly Phe Asp Val Cys Asp Leu Phe Pro Ser Trp Lys 210 215 220 Leu LeuHis Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His 225 230 235 240His Lys Tyr Asn Leu Ile Met Glu Asn Ile Ile Asn Glu His Lys Glu 245 250255 Asn His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp 260265 270 Met Ile Asp Ala Leu Leu Arg Val Lys Glu Asn Asn Glu Leu Gln Phe275 280 285 Pro Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu PheIle 290 295 300 Ala Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala LeuSer Glu 305 310 315 320 Leu Met Lys His Pro Ser Val Met Ala Lys Ala GlnAla Glu Val Arg 325 330 335 Lys Val Phe Lys Glu Asn Glu Asn Leu Asp GluAsn Asp Leu Asp Lys 340 345 350 Leu Pro Tyr Leu Lys Ser Val Ile Lys GluThr Leu Arg Met His Pro 355 360 365 Pro Val Pro Leu Leu Gly Pro Arg GluCys Arg Glu Gln Thr Glu Ile 370 375 380 Asp Gly Tyr Thr Val Pro Leu LysAla Arg Val Met Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp ProGlu Ser Trp Glu Asp Pro Glu Ser Phe Lys 405 410 415 Pro Glu Arg Phe GluAsn Ile Ser Val Asp Leu Thr Gly Asn His Tyr 420 425 430 Gln Phe Ile ProPhe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser 435 440 445 Phe Gly LeuVal Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Leu 450 455 460 Phe AspTrp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe His Thr 465 470 475 480Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu 485 490495 Ile Pro Thr Asn His Met Glu Gln Glu 500 505 207 1823 DNANICOTIANATABACUM 207 aaccaacctt ccttttctta cttagtaaaa tggatattcagtcttctcct ttcaacttaa 60 ttgctttgct actcttcatt tcatttcttt ttatcctattgaaaaagtgg aataccaaaa 120 tcccaaagtt acctccaggt ccatggagac ttccccttattggcagcctc catcacttga 180 aaggtaaact cccacaccat catcttagag atttagcccgaaaatatgga cctctcatgt 240 atttacaact tggagaagtt cctgtagttg taatatcttcgccacgtata gcaaaagctg 300 tactaaaaac tcatgatctt gcttttgcaa cgaggcctcggttcatgtcc tcggacattg 360 tgttttacaa aagcagggac atatcattcg ccccatatggcgattactgg agacaaatgc 420 gtaaaatatt aacacaagaa ctcttgagta acaagatgctcaagtcattt agcacaatcc 480 gaaaggatga gctctcgaag ctcctctcgt cgattcgtttagcaacagct tcttctgcag 540 tgaacataaa cgaaaagctt ctctggttta caagttgcatgacttgtaga ttagcctttg 600 gaaaaatatg caacgatcgt gatgaattga ttatgttaataagggagata ttagcattat 660 caggaggatt tgatgtgtgt gatttgttcc cttcatggaaattacttcac aatatgagca 720 acatgaaagc tagattgacg aatgttcacc ataagtataatctaattatg gagaatatca 780 tcaatgagca caaagagaat catgcagcag ggataaagggaaataacgag tttggtggcg 840 aagatatgat tgatgcttta ctgagggtta aggagaataatgagcttcaa tttcctatcg 900 aaaatgacaa catgaaagca gtaattctgg acttgtttattgctggaact gaaacttcat 960 atactgcaat tatatgggca ctatcagaat tgatgaagcacccaagtgtt atggccaagg 1020 cacaagctga agtgagaaaa gtcttcaaag aaaatgaaaacttggacgaa aatgatcttg 1080 acaagttgcc atacttaaaa tcagtgatca aagaaacactaaggatgcat cctccagttc 1140 ctttattagg acctagagaa tgcagagaac aaactgagattgatggatat actgtacctc 1200 ttaaagctag agtaatggtt aatgcatggg caattggaagagatcctgaa agttgggaag 1260 atcctgaaag tttcaaaccc gagcgatttg aaaatatttctgttgatctt acgggaaatc 1320 actatcagtt cattcctttc ggttcaggaa gaagaatgtgtccaggaatg tcgtttggtt 1380 tagttaacac tgggcatcct ttagctcagt tgctctatctctttgactgg aaattccctc 1440 ataaggttaa tgcagctgat tttcacacta ctgaaacaagtagagttttt gcagcaagca 1500 aagatgacct ctacttgatt ccaacaaatc acatggagcaagagtagctc taaattgaat 1560 tcttgtcttg gaacaataaa agaagaaact ccagcttggtctacattatt tccttttgct 1620 ttatattagt atgggtgtgt tcagtctctt gtttttaagggtaccctgaa agataaaggg 1680 ctatataaac cagtgagact ttttattggt tgcaaggttttagatcaagc cataagacag 1740 catattttat tccaccattt tctatcatgt ttaataaagttcctttcgtt tattgttaga 1800 aaaaaaaaaa aaaaaaaaaa aaa 1823 208 505 PRTNICOTIANATABACUM 208 Met Asp Ile Gln Ser Ser Pro Phe Asn Leu Ile Ala LeuLeu Leu Phe 1 5 10 15 Ile Ser Phe Leu Phe Ile Leu Leu Lys Lys Trp AsnThr Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Leu IleGly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His His Leu ArgAsp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly GluVal Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Ile Ala Lys Ala ValLeu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met SerSer Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala ProTyr Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln GluLeu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Phe Ser Thr Ile Arg LysAsp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Ala ThrAla Ser Ser Ala Val Asn Ile Asn Glu Lys 165 170 175 Leu Leu Trp Phe ThrSer Cys Met Thr Cys Arg Leu Ala Phe Gly Lys 180 185 190 Ile Cys Asn AspArg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu 195 200 205 Ala Leu SerGly Gly Phe Asp Val Cys Asp Leu Phe Pro Ser Trp Lys 210 215 220 Leu LeuHis Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His 225 230 235 240His Lys Tyr Asn Leu Ile Met Glu Asn Ile Ile Asn Glu His Lys Glu 245 250255 Asn His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp 260265 270 Met Ile Asp Ala Leu Leu Arg Val Lys Glu Asn Asn Glu Leu Gln Phe275 280 285 Pro Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu PheIle 290 295 300 Ala Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala LeuSer Glu 305 310 315 320 Leu Met Lys His Pro Ser Val Met Ala Lys Ala GlnAla Glu Val Arg 325 330 335 Lys Val Phe Lys Glu Asn Glu Asn Leu Asp GluAsn Asp Leu Asp Lys 340 345 350 Leu Pro Tyr Leu Lys Ser Val Ile Lys GluThr Leu Arg Met His Pro 355 360 365 Pro Val Pro Leu Leu Gly Pro Arg GluCys Arg Glu Gln Thr Glu Ile 370 375 380 Asp Gly Tyr Thr Val Pro Leu LysAla Arg Val Met Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp ProGlu Ser Trp Glu Asp Pro Glu Ser Phe Lys 405 410 415 Pro Glu Arg Phe GluAsn Ile Ser Val Asp Leu Thr Gly Asn His Tyr 420 425 430 Gln Phe Ile ProPhe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser 435 440 445 Phe Gly LeuVal Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Leu 450 455 460 Phe AspTrp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe His Thr 465 470 475 480Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu 485 490495 Ile Pro Thr Asn His Met Glu Gln Glu 500 505 209 1673 DNANICOTIANATABACUM 209 atatgcaact gagatttgaa gaataccaac taaccaaaatgcagttcttc agcctggttt 60 ccattttcct atttctatct ttcctctttt tgttaagggtatggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaatactaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagccaaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttacttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcctagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgcccctatggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaatgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccggtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccatgacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaattaaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaagtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtagatgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaatggagcgtta ggaggtgaag 840 atttaattga tgttcttcta agacttatga atgatggaggccttcaattt cctatcacca 900 acgacaacat caaagctata atttttgaca tgtttgctgccgggacagag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatccagccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaactttcgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaagacttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaacggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagagatccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaaggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcctgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcacttcgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttggagtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctcaaaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctttcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatgtaccatgttg tcg 1673 210 514 PRT NICOTIANATABACUM 210 Met Gln Leu Arg PheGlu Glu Tyr Gln Leu Thr Lys Met Gln Phe Phe 1 5 10 15 Ser Leu Val SerIle Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Val Trp Lys AsnSer Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu ProIle Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His ValLeu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu GlnLeu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala LysGlu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro SerLeu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 AlaPhe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr SerSer 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu GlnAsp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala GlyGly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu HisVal Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His HisLys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys LysAsn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu AspLeu Ile Asp Val Leu Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu GlnPhe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp MetPhe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val TrpAla Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys AlaGln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe AspGlu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 LysGlu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln CysSer 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe GlyGly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala AsnAla Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp LysLeu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr GluLeu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu ValAla Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 211 1673 DNANICOTIANATABACUM 211 atatgcaact gagatttgaa gaataccaac taaccaaaatgcagttcttc agcttggttt 60 ccattttcct atttctatct ttcctctttt tgttaaggatatggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaatactaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagccaaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttacttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcctagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgcccctatggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaatgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccggtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccatgacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaattaaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaagtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtagatgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaatggagcgtta ggaggtgaag 840 atttaattga tgttcttcta agacttatga atgatggaggccttcaattt cctatcacca 900 acgacaacat caaagccata atttttgaca tgtttgctgccgggacagag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatccagccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaactttcgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaagacttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaacggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagagatccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaaggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcctgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcacttcgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttggagtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctcaaaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctttcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatgtaccatgttg tcg 1673 212 514 PRT NICOTIANATABACUM 212 Met Gln Leu Arg PheGlu Glu Tyr Gln Leu Thr Lys Met Gln Phe Phe 1 5 10 15 Ser Leu Val SerIle Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Ile Trp Lys AsnSer Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu ProIle Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His ValLeu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu GlnLeu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala LysGlu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro SerLeu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 AlaPhe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr SerSer 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu GlnAsp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala GlyGly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu HisVal Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His HisLys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys LysAsn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu AspLeu Ile Asp Val Leu Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu GlnPhe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp MetPhe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val TrpAla Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys AlaGln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe AspGlu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 LysGlu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln CysSer 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe GlyGly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala AsnAla Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp LysLeu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr GluLeu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu ValAla Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 213 1673 DNANICOTIANATABACUM 213 atatgcaact gagatttgaa gaataccaac taaccaaagtgcagttcttc agcttggttt 60 ccattttcct atttctatct ttcctctttt tgttaaggatatggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaatactaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagccaaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttacttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcctagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgcccctatggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaatgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccggtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccatgacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaattaaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaagtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtagatgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaatggagcgtta ggaggtgaag 840 atttaattga tgttcctcta agacttatga atgatggaggccttcaattt cctatcacca 900 acgacaacat caaagctata atttttgaca tgtttgctgccgggacggag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatccagccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaactttcgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaagacttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaacggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagagatccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaaggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcctgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcacttcgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttggagtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctcaaaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctttcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatgtaccatgttg tcg 1673 214 514 PRT NICOTIANATABACUM 214 Met Gln Leu Arg PheGlu Glu Tyr Gln Leu Thr Lys Val Gln Phe Phe 1 5 10 15 Ser Leu Val SerIle Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Ile Trp Lys AsnSer Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu ProIle Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His ValLeu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu GlnLeu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala LysGlu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro SerLeu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 AlaPhe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr SerSer 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu GlnAsp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala GlyGly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu HisVal Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His HisLys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys LysAsn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu AspLeu Ile Asp Val Pro Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu GlnPhe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp MetPhe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val TrpAla Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys AlaGln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe AspGlu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 LysGlu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln CysSer 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe GlyGly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala AsnAla Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp LysLeu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr GluLeu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu ValAla Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 215 1580 DNANICOTIANATABACUM 215 gaaatgggaa atgctcacaa cagcaaaatt gcagcaatctgtttgataat tttcttggta 60 tataaagcat gggaattgtt gaagtggata tggattaagccaaagaaact ggagagttgc 120 ctcagaaaac agggactcaa aggaaattcc tacaggctattctatggaga tatgaaagaa 180 ttgtccaaaa gtctcaagga aatcaattca aagcccatcatcaatctatc aaatgaagta 240 gccccaagaa tcattcctta ttatcttgaa atcatccaaaaatatggtaa aagatgtttt 300 gtttggcaag gaccaacccc cgcaatatta ataacagagccagaattaat aaaggagata 360 tttggtaaga actatgtttt tcagaagcct aataatcccaacccactgac caagttattg 420 gctcgaggtg ttgtaagcta cgaggaagaa aaatgggcaaaacacagaaa gatcttaaac 480 cctgcctttc atatggagaa gttgaagcat atgctaccagcattttactt gagctgtagt 540 gagatgctga acaaatggga ggagattatc ccagtaaaagaatcaaatga gttggacatt 600 tggcctcatc ttcaaagaat gacaagtgat gtgatttctcgtgctgcctt tggtagtagc 660 tacgaagaag gaagaagaat atttgaactt caagaagaacaagctgagta tctaacgaag 720 acattcaatt cagtttatat cccaggttcc agattttttcccaataaaat gaacaaaaga 780 atgaaagaat gtgaaaagga agtacgagaa acaattacgtgtctaattga caacagatta 840 aaggcaaaag aagaaggcaa tggcaaggcc ctcaatgatgacctactggg tatattatta 900 gagtcaaatt ctatagaaat tgaagaacat ggtaacaagaagtttggaat gagtatacct 960 gaagtaattg aagagtgcaa attattctat tttgctggccaagagactac atcagtattg 1020 cttgtgtgga cactgatttt gttagggaga aatccagaatggcaggaacg tgctagagag 1080 gaagtttttc aagcctttgg aagtgataaa ccaacttttgacgaattata tcgcttgaaa 1140 attgtgacga tgattttgta cgagtcttta aggttatatccaccaatagc aactcgtact 1200 cgaaggacta atgaagaaac aaaattaggg gaactagatttaccaaaggg tgcactgctc 1260 tttataccaa caatcttatt acatcttgac aaggaaatttggggtgaaga tgcagatgag 1320 ttcaatccgg agagatttag cgaaggggtg gcaaaggcaacaaaggggaa aatgacatat 1380 tttccatttg gtgcaggacc gcgaaaatgc attgggcaaaacttcgcgat tttggaagca 1440 aaaatggcta tagctatgat tctacaacgc ttctccttcgagctctctcc atcttataca 1500 cactctccat acactgtggt cactttgaaa cccaaatatggtgctcccct aataatgcac 1560 aggctgtagt cctgtgagaa 1580 216 521 PRTNICOTIANATABACUM 216 Met Gly Asn Ala His Asn Ser Lys Ile Ala Ala Ile CysLeu Ile Ile 1 5 10 15 Phe Leu Val Tyr Lys Ala Trp Glu Leu Leu Lys TrpIle Trp Ile Lys 20 25 30 Pro Lys Lys Leu Glu Ser Cys Leu Arg Lys Gln GlyLeu Lys Gly Asn 35 40 45 Ser Tyr Arg Leu Phe Tyr Gly Asp Met Lys Glu LeuSer Lys Ser Leu 50 55 60 Lys Glu Ile Asn Ser Lys Pro Ile Ile Asn Leu SerAsn Glu Val Ala 65 70 75 80 Pro Arg Ile Ile Pro Tyr Tyr Leu Glu Ile IleGln Lys Tyr Gly Lys 85 90 95 Arg Cys Phe Val Trp Gln Gly Pro Thr Pro AlaIle Leu Ile Thr Glu 100 105 110 Pro Glu Leu Ile Lys Glu Ile Phe Gly LysAsn Tyr Val Phe Gln Lys 115 120 125 Pro Asn Asn Pro Asn Pro Leu Thr LysLeu Leu Ala Arg Gly Val Val 130 135 140 Ser Tyr Glu Glu Glu Lys Trp AlaLys His Arg Lys Ile Leu Asn Pro 145 150 155 160 Ala Phe His Met Glu LysLeu Lys His Met Leu Pro Ala Phe Tyr Leu 165 170 175 Ser Cys Ser Glu MetLeu Asn Lys Trp Glu Glu Ile Ile Pro Val Lys 180 185 190 Glu Ser Asn GluLeu Asp Ile Trp Pro His Leu Gln Arg Met Thr Ser 195 200 205 Asp Val IleSer Arg Ala Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Arg IlePhe Glu Leu Gln Glu Glu Gln Ala Glu Tyr Leu Thr Lys Thr 225 230 235 240Phe Asn Ser Val Tyr Ile Pro Gly Ser Arg Phe Phe Pro Asn Lys Met 245 250255 Asn Lys Arg Met Lys Glu Cys Glu Lys Glu Val Arg Glu Thr Ile Thr 260265 270 Cys Leu Ile Asp Asn Arg Leu Lys Ala Lys Glu Glu Gly Asn Gly Lys275 280 285 Ala Leu Asn Asp Asp Leu Leu Gly Ile Leu Leu Glu Ser Asn SerIle 290 295 300 Glu Ile Glu Glu His Gly Asn Lys Lys Phe Gly Met Ser IlePro Glu 305 310 315 320 Val Ile Glu Glu Cys Lys Leu Phe Tyr Phe Ala GlyGln Glu Thr Thr 325 330 335 Ser Val Leu Leu Val Trp Thr Leu Ile Leu LeuGly Arg Asn Pro Glu 340 345 350 Trp Gln Glu Arg Ala Arg Glu Glu Val PheGln Ala Phe Gly Ser Asp 355 360 365 Lys Pro Thr Phe Asp Glu Leu Tyr ArgLeu Lys Ile Val Thr Met Ile 370 375 380 Leu Tyr Glu Ser Leu Arg Leu TyrPro Pro Ile Ala Thr Arg Thr Arg 385 390 395 400 Arg Thr Asn Glu Glu ThrLys Leu Gly Glu Leu Asp Leu Pro Lys Gly 405 410 415 Ala Leu Leu Phe IlePro Thr Ile Leu Leu His Leu Asp Lys Glu Ile 420 425 430 Trp Gly Glu AspAla Asp Glu Phe Asn Pro Glu Arg Phe Ser Glu Gly 435 440 445 Val Ala LysAla Thr Lys Gly Lys Met Thr Tyr Phe Pro Phe Gly Ala 450 455 460 Gly ProArg Lys Cys Ile Gly Gln Asn Phe Ala Ile Leu Glu Ala Lys 465 470 475 480Met Ala Ile Ala Met Ile Leu Gln Arg Phe Ser Phe Glu Leu Ser Pro 485 490495 Ser Tyr Thr His Ser Pro Tyr Thr Val Val Thr Leu Lys Pro Lys Tyr 500505 510 Gly Ala Pro Leu Ile Met His Arg Leu 515 520 217 1587 DNANICOTIANATABACUM 217 atgctttctc ccatagaagc ctttgtagga ctagtaaccttcacatttct cttatacttc 60 ctatggacaa aaaaatctca aaaacttcca aaacccttaccaccgaaaat ccccggagga 120 tggccggtaa tcggccatct ttttcacttc aataacgacggcgacgaccg tccattagct 180 cgaaagctcg gagacttagc tgataaatac ggccccgttttcacttttcg gctaggtctt 240 ccccttgtgc tagttgtaag cagttacgaa gctataaaagattgcttctc tacaaatgat 300 gccattttct ccaatcgtcc agctcttctt tacggcgaataccttggcta caataataca 360 atgctttttc tagcaaatta cggaccttac tggcgaaaaaatcgtaaatt agtcattcag 420 gaagttctct ctgctagtcg tctcgaaaaa ttcaaacaagtgagattcac cagaattcaa 480 acgagcatta agaatttata cactcgaatt aatggaaattcgagtacgat aaatctaact 540 gattggttag aagaattgaa ttttggtctg atcgtgaaaatgatcgctgg gaaaaattat 600 gaatccggta aaggagatga acaagtggaa agatttaagaatgcgtttaa ggattttatg 660 gttttatcaa tggaatttgt attatgggat gcatttccaattccattatt taaatgggtg 720 gattttcaag gtcatattaa ggcaatgaaa aggacatttaaggatataga ttctgttttt 780 cagaactggt tagaggaaca tattaataaa agagaaaaaatagaggttgg tgcagaaggg 840 aatgaacaag atttcattga tgtggtgctt tcaaaattgagtaaagaata tcttgatgaa 900 ggttactctc gtgatactgt cattaaagca acagtttttagtttggtctt ggatgcagca 960 gacacagttg ctcttcacat aaattgggga atgacattattgataaacaa tcaaaatgcc 1020 ttgatgaaag cacaagaaga gatagacaca aaagttggtaaggatagatg ggtagaagag 1080 agtgatatta aggatttagt atacctccaa gctattgttaaaaaggtgtt acgattatat 1140 ccaccaggac ctttgttagt accacatgaa aatgtaaaggattgtgttgt tagtggatat 1200 cacattccta aagggactag attattcgca aacgtcatgaaactgcagcg cgatcctaaa 1260 ctcttgtcaa atcctgataa gttcgatcca gagagattcatcgctggtga tattgacttc 1320 cgtggtcacc actatgagtt tatcccattt ggttctggaagacgatcttg tccggggatg 1380 acttatgcat tgcaagtgga acacctaaca atggcacatttaatccaggg tttcaattac 1440 aaaactccaa atgacgaggc cttggatatg aaggaaggtgcaggcataac aatacgtaag 1500 gtaaatccag tggaattgat aataacgcct cgcttggcacctgagcttta ctaaaaccta 1560 agatgtttca tcttggttga tcattgt 1587 218 517PRT NICOTIANATABACUM 218 Met Leu Ser Pro Ile Glu Ala Phe Val Gly Leu ValThr Phe Thr Phe 1 5 10 15 Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser GlnLys Leu Pro Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro ValIle Gly His Leu Phe 35 40 45 His Phe Asn Asn Asp Gly Asp Asp Arg Pro LeuAla Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe ThrPhe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr GluAla Ile Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn ArgPro Ala Leu Leu Tyr Gly 100 105 110 Glu Tyr Leu Gly Tyr Asn Asn Thr MetLeu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg LysLeu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys PheLys Gln Val Arg Phe Thr Arg Ile Gln 145 150 155 160 Thr Ser Ile Lys AsnLeu Tyr Thr Arg Ile Asn Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu ThrAsp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met IleAla Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val GluArg Phe Lys Asn Ala Phe Lys Asp Phe Met Val Leu Ser Met 210 215 220 GluPhe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235240 Asp Phe Gln Gly His Ile Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu260 265 270 Lys Ile Glu Val Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile AspVal 275 280 285 Val Leu Ser Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly TyrSer Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val LeuAsp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly MetThr Leu Leu Ile Asn 325 330 335 Asn Gln Asn Ala Leu Met Lys Ala Gln GluGlu Ile Asp Thr Lys Val 340 345 350 Gly Lys Asp Arg Trp Val Glu Glu SerAsp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Lys ValLeu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu AsnVal Lys Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys GlyThr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro LysLeu Leu Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg 420 425 430 Phe Ile AlaGly Asp Ile Asp Phe Arg Gly His His Tyr Glu Phe Ile 435 440 445 Pro PheGly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 GlnVal Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475480 Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys Glu Gly Ala Gly Ile 485490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu500 505 510 Ala Pro Glu Leu Tyr 515 219 1634 DNA NICOTIANATABACUM 219gggagaaggc cttcaatatg gagataccat attacagctt aaaaattgca atttcttcat 60ttgcaattat ctttgtacta agatgggcat ggaaaatctt gaattatgtg tggttaaaac 120caaaagaatt ggagaaatac ctcagacagc agggtttcaa aggaaactct tacaaattct 180tgtttgggga tatgaaagag acgaagaaaa tgggtgaaga agctatgtct aagccaatca 240atttctctca tgacatgatt tggcctagag ttatgccatt catccacaaa accatcacca 300attatggtaa gaattgtatt gtgtggtttg ggccaagacc agcagtcctg atcacagacc 360cggaacttgt aaaggaggtg ctaacgaaga atttcgtcta tcagaagccg cttggcaatc 420cactcacaaa gttggcagca actggaattg caggctatga aacagataaa tgggctacac 480atagaaggct tctcaatcct gcttttcacc ttgacaagtt gaagcatatg ctacctgcat 540tccaatttac tgctagtgag atgttgagca aattggagaa agttgtttca ccaaacggaa 600cagagataga tgtgtggcca tatttacaaa ctttgacaag tgatgccatt tcaagaactg 660cgtttggaag tagttatgaa gaaggaagaa agatttttga ccttcaaaaa gaacaacttt 720cactaattct agaagtttca cgcacaatat atattccagg atggaggttt ttgccaacga 780aaaggaacaa aaggatgaag caaatattta atgaagtacg agcactggta tttggaatta 840ttaagaaaag gatgagtatg attgaaaatg gagaagcacc tgatgattta ttgggaatat 900tattggcatc caatttaaaa gaaatccaac aacatggaaa caacaagaaa tttggtatga 960gtattgatga ggtgattgaa gagtgtaaac tcttctattt tgctgggcaa gagactactt 1020catctttact tgtatggact atgattttgt tgtgcaaata tcctaattgg caagataaag 1080ctagagaaga ggttttgcaa gtgtttggga gtagggaagt tgactatgac aagttgaatc 1140agctaaaaat agtaactatg atcttaaacg aggtcttaag gttgtatcca gcaggatatg 1200tgattaatcg aatggtaaac aaagaaacaa agttagggaa tttgtgttta ccagccggcg 1260tacagctcgt gttaccaaca atgttgttgc aacatgatac tgaaatatgg ggagatgatg 1320caatggagtt caatccagag agatttagtg atggaatatc caaagcaaca aaaggaaaac 1380ttgtgttttt tccatttagt tggggtccaa gaatatgtat tgggcaaaat tttgctatgt 1440tagaggctaa aatggcaatg gctatgattc tgaaaaccta tgcatttgaa ctctctccat 1500cttatgctca tgctcctcat ccactactac ttcaacctca atatggtgct caattaattt 1560tgtacaagtt gtagatatgg tcaatctgga acttgttatg gaacttttat catcgtaatc 1620aaccatattg aggg 1634 220 508 PRT NICOTIANATABACUM 220 Met Glu Ile ProTyr Tyr Ser Leu Lys Ile Ala Ile Ser Ser Phe Ala 1 5 10 15 Ile Ile PheVal Leu Arg Trp Ala Trp Lys Ile Leu Asn Tyr Val Trp 20 25 30 Leu Lys ProLys Glu Leu Glu Lys Tyr Leu Arg Gln Gln Gly Phe Lys 35 40 45 Gly Asn SerTyr Lys Phe Leu Phe Gly Asp Met Lys Glu Thr Lys Lys 50 55 60 Met Gly GluGlu Ala Met Ser Lys Pro Ile Asn Phe Ser His Asp Met 65 70 75 80 Ile TrpPro Arg Val Met Pro Phe Ile His Lys Thr Ile Thr Asn Tyr 85 90 95 Gly LysAsn Cys Ile Val Trp Phe Gly Pro Arg Pro Ala Val Leu Ile 100 105 110 ThrAsp Pro Glu Leu Val Lys Glu Pro Leu Gly Asn Pro Leu Thr Lys 115 120 125Leu Ala Ala Thr Gly Ile Ala Gly Tyr Glu Thr Asp Lys Trp Ala Thr 130 135140 His Arg Arg Leu Leu Asn Pro Ala Phe His Leu Asp Lys Leu Lys His 145150 155 160 Met Leu Pro Ala Phe Gln Phe Thr Ala Ser Glu Met Leu Ser LysLeu 165 170 175 Glu Lys Val Val Ser Pro Asn Gly Thr Glu Ile Asp Val TrpPro Tyr 180 185 190 Leu Gln Thr Leu Thr Ser Asp Ala Ile Ser Arg Thr AlaPhe Gly Ser 195 200 205 Ser Tyr Glu Glu Gly Arg Lys Ile Phe Asp Leu GlnLys Glu Gln Leu 210 215 220 Ser Leu Ile Leu Glu Val Ser Arg Thr Ile TyrIle Pro Gly Trp Arg 225 230 235 240 Phe Leu Pro Thr Lys Arg Asn Lys ArgMet Lys Gln Ile Phe Asn Glu 245 250 255 Val Arg Ala Leu Val Phe Gly IleIle Lys Lys Arg Met Ser Met Ile 260 265 270 Glu Asn Gly Glu Ala Pro AspAsp Leu Leu Gly Ile Leu Leu Ala Ser 275 280 285 Asn Leu Lys Glu Ile GlnGln His Gly Asn Asn Lys Lys Phe Gly Met 290 295 300 Ser Ile Asp Glu ValIle Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly 305 310 315 320 Gln Glu ThrThr Ser Ser Leu Leu Val Trp Thr Met Ile Leu Leu Cys 325 330 335 Lys TyrPro Asn Trp Gln Asp Lys Ala Arg Glu Glu Val Leu Gln Val 340 345 350 PheGly Ser Arg Glu Val Asp Tyr Asp Lys Leu Asn Gln Leu Lys Ile 355 360 365Val Thr Met Ile Leu Asn Glu Val Leu Arg Leu Tyr Pro Ala Gly Tyr 370 375380 Val Ile Asn Arg Met Val Asn Lys Glu Thr Lys Leu Gly Asn Leu Cys 385390 395 400 Leu Pro Ala Gly Val Gln Leu Val Leu Pro Thr Met Leu Leu GlnHis 405 410 415 Asp Thr Glu Ile Trp Gly Asp Asp Ala Met Glu Phe Asn ProGlu Arg 420 425 430 Phe Ser Asp Gly Ile Ser Lys Ala Thr Lys Gly Lys LeuVal Phe Phe 435 440 445 Pro Phe Ser Trp Gly Pro Arg Ile Cys Ile Gly GlnAsn Phe Ala Met 450 455 460 Leu Glu Ala Lys Met Ala Met Ala Met Ile LeuLys Thr Tyr Ala Phe 465 470 475 480 Glu Leu Ser Pro Ser Tyr Ala His AlaPro His Pro Leu Leu Leu Gln 485 490 495 Pro Gln Tyr Gly Ala Gln Leu IleLeu Tyr Lys Leu 500 505 221 1593 DNA NICOTIANATABACUM 221 atggtttttcccatagaagc ctttgtagga ctagtaacct tcacatttct cttatacttc 60 ctatggacaaaaaaatctca aaaacttcca aaacccttac caccgaaaat ccccggagga 120 tggccggtaatcggccacct ttttcacttc aataacgacg gcgacgaccg tccattagct 180 cgaaaactcggagacttagc tgataaatac ggccccgttt tcacttttcg gctaggtctt 240 ccccttgtgctagttgtaag cagttacgaa gctacaaaag attgcttctc tacaaatgac 300 gccattttctccaatcgtcc agcttttctt tacggcgaat accttggcta caataataca 360 atgctttttctagcaaatta cggaccttac tggcgaaaaa atcgtaaatt agtcattcag 420 gaagttctctctgctagtcg tctcgaaaaa ttcaaacaag tgagattcac cagaattcaa 480 acgagcattaagaatttata cactcgaatt aatggaaatt cgagtacgat aaatctaact 540 gattggttagaagaattgaa ttttggtctg atcgtgaaaa tgatcgctgg gaaaaattat 600 gaatccggtaaaggagatga acaagtggaa agatttaaga atgcgtttaa ggattttatg 660 gttttatcaatggaatttgt attatgggat gcatttccaa ttccattatt taaatgggtg 720 gattttcaaggtcatattaa gacaatgaaa aggacattta aggatataga ttctgttttt 780 cagaactggttagaggaaca tattaataaa agagaaaaaa tggaggttgg tgcagaaggg 840 aatgaacaagatttcattga tgtggtgctt tcaaaattga gtaaagaata tcttgatgaa 900 ggttactctcgtgatactgt cattaaagca acagttttta gtttggtctt ggatgcagca 960 gacacagttgctcttcacat aaattgggga atgacattat tgataaacaa tcaaaatgcc 1020 ttgatgaaagcacaagaaga gatagacaca aaagttggta aggatagatg ggtagaagag 1080 agtgatattaaggatttagt atacctccaa gctattgtta aaaaggtgtt acgattatat 1140 ccaccaggacctttgttagt accacatgaa aatgtaaagg attgtgttgt tagtggatat 1200 cacattcctaaagggactag attattcgca aacgtcatga aactgcagcg cgatcctaaa 1260 ctcttgtcaaatcctgataa gttcgatcca gagagattca tcgctggtga tattgacttc 1320 cgtggtcaccactatgagtt tatcccatct ggttctggaa gacgatcttg tccggggatg 1380 acttatgcattgcaagtgga acacctaaca atggcacatt taatccaggg tttcaattac 1440 aaaactccaaatgacgaggt cttggatatg aaggaaggtg caggcataac aatacgtaag 1500 gtaaatccagtggaattgat aataacgcct cgcttggcac ctgagcttta ctaaaaccta 1560 agatctttcatcttggttga tcattgttta ata 1593 222 517 PRT NICOTIANATABACUM 222 Met ValPhe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 LeuLeu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro 20 25 30 LeuPro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 HisPhe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 AspLeu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Thr Lys Asp Cys Phe 85 90 95Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105110 Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr Gly 115120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg IleGln 145 150 155 160 Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly AsnSer Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn PheGly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser GlyLys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Asn Ala Phe Lys AspPhe Met Val Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe ProIle Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Ile LysThr Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln AsnTrp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val GlyAla Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser LysLeu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg 290 295 300 Asp Thr ValIle Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 AspThr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn 325 330 335Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345350 Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355360 365 Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro Pro Gly Pro370 375 380 Leu Leu Val Pro His Glu Asn Val Lys Asp Cys Val Val Ser GlyTyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val MetLys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Leu Ser Asn Pro Asp Lys PheAsp Pro Glu Arg 420 425 430 Phe Ile Ala Gly Asp Ile Asp Phe Arg Gly HisHis Tyr Glu Phe Ile 435 440 445 Pro Ser Gly Ser Gly Arg Arg Ser Cys ProGly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala HisLeu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Lys Thr Pro Asn Asp Glu ValLeu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val AsnPro Val Glu Leu Ile Ile Thr Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr515 223 1580 DNA NICOTIANATABACUM 223 atgctttctc ccatagaagc cattgtaggactagtaacct tcacatttct cttcttcttc 60 ctatggacaa aaaaatctca aaaaccttcaaaacccttac caccgaaaat ccccggagga 120 tggccggtaa tcggccatct tttccacttcaatgacgacg gcgacgaccg tccattagct 180 cgaaaactcg gagacttagc tgacaaatacggccccgttt tcacttttcg gctaggcctt 240 ccccttgtct tagttgtaag cagttacgaagctgtaaaag actgtttctc cacaaatgac 300 gccatttttt ccaatcgtcc agcttttctttacggcgatt accttggcta caataatgcc 360 atgctatttt tggccaatta cggaccttactggcgaaaaa atcgaaaatt agttattcag 420 gaagttctct ccgctagtcg tctcgaaaaattcaaacacg tgagatttgc aagaattcaa 480 gcgagcatga agaatttata tactcgaattgatggaaatt cgagtacgat aaatttaact 540 gattggttag aagaattgaa ttttggtctgatcgtgaaga tgatcgctgg aaaaaattat 600 gaatccggta aaggagatga acaagtggagagatttaaga aagcgtttaa ggattttatg 660 attttatcaa tggagtttgt gttatgggatgcatttccaa ttccattatt taaatgggtg 720 gattttcaag ggcatgttaa ggctatgaaaaggactttta aagatataga ttctgttttt 780 cagaattggt tagaggaaca tattaataaaagagaaaaaa tggaggttaa tgcagaaggg 840 aatgaacaag atttcattga tgtggtgctttcaaaaatga gtaatgaata tcttggtgaa 900 ggttactctc gtgatactgt cattgaagcaacggtgttta gtttggtctt ggatgcagca 960 gacacagttg ctcttcacat aaattggggaatggcattat tgataaacaa tcaaaaggcc 1020 ttgacgaaag cacaagaaga gatagacacaaaagtttgta aggacagatg ggtagaagag 1080 agtgatatta aggatttggt atacctccaagctattgtta aagaagtgtt acgattatat 1140 ccaccaggac ctttgttagt accacacgaaaatgtagaag attgtgttgt tagtggatat 1200 cacattccta aagggacaag attattcgcaaacgtcatga aactgcaacg tgatcctaaa 1260 ctctggtctg atcctgatac tttcgatccagagagattca ttgctactga tattgacttt 1320 cgtggtcagt actataagta tatcccgtttggtcctggaa gacgatcttg tccagggatg 1380 acttatgcat tgcaagtgga acacttaacaatggcacatt tgatccaagg tttcaattac 1440 agaactccaa atgacgagcc cttggatatgaaggaaggtg caggcataac tatacgtaag 1500 gtaaatcctg tggaactgat aatagcgcctcgcctggcac ctgagcttta ttaaaaccta 1560 agatgtttca tcttggttga 1580 224 517PRT NICOTIANATABACUM 224 Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu ValThr Phe Thr Phe 1 5 10 15 Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser GlnLys Pro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro ValIle Gly His Leu Phe 35 40 45 His Phe Asn Asp Asp Gly Asp Asp Arg Pro LeuAla Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe ThrPhe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr GluAla Val Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn ArgPro Ala Phe Leu Tyr Gly 100 105 110 Asp Tyr Leu Gly Tyr Asn Asn Ala MetLeu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg LysLeu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys PheLys His Val Arg Phe Ala Arg Ile Gln 145 150 155 160 Ala Ser Met Lys AsnLeu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu ThrAsp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met IleAla Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val GluArg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met 210 215 220 GluPhe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235240 Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu260 265 270 Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile AspVal 275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly TyrSer Arg 290 295 300 Asp Thr Val Ile Glu Ala Thr Val Phe Ser Leu Val LeuAsp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly MetAla Leu Leu Ile Asn 325 330 335 Asn Gln Lys Ala Leu Thr Lys Ala Gln GluGlu Ile Asp Thr Lys Val 340 345 350 Cys Lys Asp Arg Trp Val Glu Glu SerAsp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu ValLeu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu AsnVal Glu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys GlyThr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro LysLeu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg 420 425 430 Phe Ile AlaThr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile 435 440 445 Pro PheGly Pro Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 GlnVal Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475480 Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile 485490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu500 505 510 Ala Pro Glu Leu Tyr 515 225 1639 DNA NICOTIANATABACUM 225tttcatatac ctttagtact cttgaaattt tcaaataatg gtttatcttc tttctcccat 60agaagccatt gtaggatttg taaccttttc atttctattc tactttctat ggaccaaaaa 120acaatcaaaa atcttaaacc cactacctcc aaaaatccca ggtggatggc cagtaatcgg 180ccatctcttt tatttcaaga acaatggcga tgaagatcgc catttttctc aaaaactcgg 240tgacttagct gacaaatatg gtcccgtctt cactttccgg ttagggtttc gccgtttctt 300ggcggtgagt agttatgaag ctatgaaaga atgcttcact accaatgata tccatttcgc 360cgatcggcca tctttactct acggagaata cctttgctat aataacgcca tgcttgctgt 420tgccaaatat ggcccttact ggaaaaaaaa tcgaaagtta gtcaatcaag aagttctctc 480cgttagtcgg ctcgaaaaat tcaaacatgt tagattttct ataattcaga aaaatattaa 540acaattgtat aattgtgatt caccaatggt gaagataaac cttagtgatt ggatagataa 600attgacattc gacatcattt tgaaaatggt tgttgggaag aactataata atggacatgg 660agaaatactc aaagttgctt ttcagaaatt catggttcaa gctatggaga tggagctcta 720tgatgttttt cacattccat ttttcaagtg gttggatctt acagggaata ttaaggctat 780gaaacaaact ttcaaagaca ttgataatat tatccaaggt tggttagatg agcacattaa 840gaagagagaa acaaaggatg ttggaggtga aaacgaacaa gattttatag atgtggtgct 900ttccaagatg agcgacgaac atcttggcga gggttactct catgacacaa ccatcaaagc 960aactgtattc actttggtct tggatgcaac agacacactt gcacttcata taaagtgggt 1020aatggcgtta atgataaaca ataagcatgt catgaagaaa gcacaagaag agatggacac 1080aattgttggt agagatagat gggtagaaga gagtgatatc aagaatttgg tgtatctcca 1140agcaattgtc aaagaagtat tacgattaca tccacccgca cctttgtcag tgcaacacct 1200atctgtagaa gattgtgttg tcaatgggta ccatattcct aaggggactg cactacttac 1260caatattatg aaactacagc gagatcctca aacatggcca aatcctgata aattcgatcc 1320agagagattc ctgacgactc atgctactat tgactaccgc gggcagcact atgagtcgat 1380cccctttggt acggggagac gagcttgtcc cgcgatgaat tattcattgc aagtggaaca 1440cctttcaatt gctcatatga tccaaggttt cagttttgca actacgacca atgagccttt 1500ggatatgaaa caaggtgtgg gtttaacttt accaaagaag actgatgttg aagtgctaat 1560tacacctcgc cttcctccta cgctttatca atattaagat gttttgttgt cgggattcgt 1620tctgatcaat ccctcaatg 1639 226 519 PRT NICOTIANATABACUM 226 Met Val TyrLeu Leu Ser Pro Ile Glu Ala Ile Val Gly Phe Val Thr 1 5 10 15 Phe SerPhe Leu Phe Tyr Phe Leu Trp Thr Lys Lys Gln Ser Lys Ile 20 25 30 Leu AsnPro Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly 35 40 45 His LeuPhe Tyr Phe Lys Asn Asn Gly Asp Glu Asp Arg His Phe Ser 50 55 60 Gln LysLeu Gly Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe 65 70 75 80 ArgLeu Gly Phe Arg Arg Phe Leu Ala Val Ser Ser Tyr Glu Ala Met 85 90 95 LysGlu Cys Phe Thr Thr Asn Asp Ile His Phe Ala Asp Arg Pro Ser 100 105 110Leu Leu Tyr Gly Glu Tyr Leu Cys Tyr Asn Asn Ala Met Leu Ala Val 115 120125 Ala Lys Tyr Gly Pro Tyr Trp Lys Lys Asn Arg Lys Leu Val Asn Gln 130135 140 Glu Val Leu Ser Val Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe145 150 155 160 Ser Ile Ile Gln Lys Asn Ile Lys Gln Leu Tyr Asn Cys AspSer Pro 165 170 175 Met Val Lys Ile Asn Leu Ser Asp Trp Ile Asp Lys LeuThr Phe Asp 180 185 190 Ile Ile Leu Lys Met Val Val Gly Lys Asn Tyr AsnAsn Gly His Gly 195 200 205 Glu Ile Leu Lys Val Ala Phe Gln Lys Phe MetVal Gln Ala Met Glu 210 215 220 Met Glu Leu Tyr Asp Val Phe His Ile ProPhe Phe Lys Trp Leu Asp 225 230 235 240 Leu Thr Gly Asn Ile Lys Ala MetLys Gln Thr Phe Lys Asp Ile Asp 245 250 255 Asn Ile Ile Gln Gly Trp LeuAsp Glu His Ile Lys Lys Arg Glu Thr 260 265 270 Lys Asp Val Gly Gly GluAsn Glu Gln Asp Phe Ile Asp Val Val Leu 275 280 285 Ser Lys Met Ser AspGlu His Leu Gly Glu Gly Tyr Ser His Asp Thr 290 295 300 Thr Ile Lys AlaThr Val Phe Thr Leu Val Leu Asp Ala Thr Asp Thr 305 310 315 320 Leu AlaLeu His Ile Lys Trp Val Met Ala Leu Met Ile Asn Asn Lys 325 330 335 HisVal Met Lys Lys Ala Gln Glu Glu Met Asp Thr Ile Val Gly Arg 340 345 350Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asn Leu Val Tyr Leu Gln 355 360365 Ala Ile Val Lys Glu Val Leu Arg Leu His Pro Pro Ala Pro Leu Ser 370375 380 Val Gln His Leu Ser Val Glu Asp Cys Val Val Asn Gly Tyr His Ile385 390 395 400 Pro Lys Gly Thr Ala Leu Leu Thr Asn Ile Met Lys Leu GlnArg Asp 405 410 415 Pro Gln Thr Trp Pro Asn Pro Asp Lys Phe Asp Pro GluArg Phe Leu 420 425 430 Thr Thr His Ala Thr Ile Asp Tyr Arg Gly Gln HisTyr Glu Ser Ile 435 440 445 Pro Phe Gly Thr Gly Arg Arg Ala Cys Pro AlaMet Asn Tyr Ser Leu 450 455 460 Gln Val Glu His Leu Ser Ile Ala His MetIle Gln Gly Phe Ser Phe 465 470 475 480 Ala Thr Thr Thr Asn Glu Pro LeuAsp Met Lys Gln Gly Val Gly Leu 485 490 495 Thr Leu Pro Lys Lys Thr AspVal Glu Val Leu Ile Thr Pro Arg Leu 500 505 510 Pro Pro Thr Leu Tyr GlnTyr 515 227 1610 DNA NICOTIANATABACUM 227 ctttttctcc ccaaaaaagagctcatttcc cttgtcccca aaaatggatc ttctcttact 60 agagaagacc ttaattggtctcttctttgc cattttaatc gctataattg tctctagact 120 tcgttcaaag cgttttaagcttcccccagg accaatccca gtaccagttt ttggtaattg 180 gcttcaagtt ggtgatgatttaaaccacag aaatcttact gattttgcca aaaaatttgg 240 tgatcttttc ttgttaagaatgggccagcg taatttagtt gttgtgtcat ctcctgaatt 300 agctaaagaa gttttacacacacaaggtgt tgaatttggt tcaagaacaa gaaatgttgt 360 atttgatatt tttactggaaaaggtcaaga tatggttttt actgtatatg gtgaacactg 420 gagaaaaatg aggagaattatgactgtacc attttttact aataaagttg tgcagcaata 480 tagagggggg tgggagtttgaagtggcaag tgtaattgag gatgtgaaga aaaatcctga 540 atctgctact aatgggattgtattaaggag gagattacaa ttgatgatgt ataataatat 600 gtttaggatt atgtttgataggagatttga gagtgaagat gatcctttgt ttgttaagct 660 taaggctttg aatggtgaaaggagtagatt ggctcagagt tttgagtata attatggtga 720 ttttattccc attttgaggccttttttgag aggttatttg aagatctgta aagaagttaa 780 ggagaagagg ctgcagcttttcaaagatta ctttgttgat gaaagaaaga agctttcaaa 840 taccaagagc ttggacagcaatgctctgaa atgtgcgatt gatcacattc ttgaggctca 900 acagaagggg gagatcaatgaggacaacgt tctttacatt gttgaaaaca tcaatgttgc 960 tgctatagaa accacattatggtcaattga gtggggtatc gccgagttag tcaaccaccc 1020 tcacatccaa aagaaactccgcgacgagat tgacacagtt cttggcccag gagtgcaagt 1080 gactgaacca gacacccacaagcttccata ccttcaggct gtgatcaagg agacgcttcg 1140 tctccgtatg gcaattcctctattagtccc acacatgaac cttcacgatg caaagcttgg 1200 cgggtttgat attccagcagagagcaaaat cttggttaac gcttggtggc tagctaacaa 1260 cccggctcat tggaagaaacccgaagagtt cagacccgag aggttcttcg aagaggagaa 1320 gcacgttgag gccaatggcaatgacttcag atatcttccg tttggcgttg gtaggaggag 1380 ttgccctgga attatacttgcattgccaat tcttggcatt actttgggac gtttggttca 1440 gaactttgag ctgttgcctcctccaggcca gtcgaagctc gacaccacag agaaaggtgg 1500 acagttcagt ctccatattttgaagcattc caccattgtg ttgaaaccaa ggtcttgctg 1560 aactttctga tcctaatcaattaaggggtt gaagaaattt tataattatg 1610 228 505 PRT NICOTIANATABACUM 228Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 1015 Ile Leu Ile Ala Ile Ile Val Ser Arg Leu Arg Ser Lys Arg Phe Lys 20 2530 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 4045 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Phe Ala Lys Lys 50 5560 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 7075 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 8590 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp ArgLys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys ValVal Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser ValIle Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn GlyIle Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn MetPhe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp ProLeu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg LeuAla Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile LeuArg Pro Phe Leu Arg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu ValLys Glu Lys Arg Leu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp GluArg Lys Lys Leu Ser Asn Thr Lys Ser Leu Asp Ser 260 265 270 Asn Ala LeuLys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly GluIle Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 ValAla Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315320 Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325330 335 Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His340 345 350 Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg LeuArg 355 360 365 Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His AspAla Lys 370 375 380 Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile LeuVal Asn Ala 385 390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp LysLys Pro Glu Glu Phe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu LysHis Val Glu Ala Asn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe GlyVal Gly Arg Arg Ser Cys Pro 435 440 445 Gly Ile Ile Leu Ala Leu Pro IleLeu Gly Ile Thr Leu Gly Arg Leu 450 455 460 Val Gln Asn Phe Glu Leu LeuPro Pro Pro Gly Gln Ser Lys Leu Asp 465 470 475 480 Thr Thr Glu Lys GlyGly Gln Phe Ser Leu His Ile Leu Lys His Ser 485 490 495 Thr Ile Val LeuLys Pro Arg Ser Cys 500 505 229 1595 DNA NICOTIANATABACUM 229 aacaataaaaatggagacat tatttaacat caaagttgca gtttcattag taattgtgat 60 aatttttctgagatgggtat ggaaattctt gaattgggtg tggattcaac caaagaaaat 120 ggaaaaaagactaaaaatgg aaggtttcaa aggaagctca tataagctat tatttggaga 180 tatgaaagaaataaatacaa tggttgaaga agccaaaacc aagcctatga attttaccaa 240 tgattatgtggctagagtct tgcctcactt cacaaagttg atgctccaat atggcaagaa 300 tagctttatgtggttagggc caaaaccaac aatgtttatc acagaccctg aactaataag 360 ggagatcttgtcaaaaagtt acatatacca ggagattcaa ggcaatccaa tcactaagtt 420 gctagcacaaggactagtaa gttatgaagc agagaaatgg gctaagcata gaaaaattat 480 caatcctgcatttcaccttg acaagttgaa gcatatgcta ccatcattct acttgagttg 540 ttgtgacatgctcagaaaat gggaaagtat agcttcatca gagggatcag aaatagacgt 600 gtggccttttctggaaacgt tgacaagcga tgctatttca agaacagctt ttggtagtaa 660 ctatgaagacgggagacaga tatttgagct tcaaaaagaa caagctgagt tgattttaca 720 agcagcgcgatggctttaca tccccggatg gaggtttgtg ccaacaaaga ggaacaagag 780 gatgaagcaaatcgctaaag aagtacgatc attagtgttg ggaataatca ataagagaat 840 aagggaaatgaaagcagggg aagctgcaaa agatgactta ctgggaatac tattggaatc 900 taatttcaaagaaatccaaa tgcacggaaa caagaacttt ggcatgacta tcgacgaagt 960 gattgaagagtgcaagttat tttactttgc tgggcaagaa actacttcag ttttgcttgt 1020 ttggactttgattttactga gtaagcatgt cgattggcaa gaaagagcta gagaagaagt 1080 tcatcaagtctttggaagta acaaacctga ttatgacgca ttgaatcagt tgaaagttgt 1140 aacgatgatattcaacgagg ttttaaggtt gtacccaccg ggaattacca taagtcgaac 1200 tgtacacgaggataccaaat tagggaactt gtcattgcca gcagggatac agcttgtgtt 1260 acctgcaatttggttgcatc atgacaatga aatatgggga gatgatgcaa aggagttcaa 1320 accagagaggtttagtgaag gagttaataa agcaacaaag ggtaaatttg catattttcc 1380 atttagttggggaccaagaa tatgtgttgg actgaatttt gcaatgttag aggcaaaaat 1440 ggcacttgcattgattctac aacactatgc ttttgagctc tctccatctt atgcacatgc 1500 tcctcatacaattatcactc tgcaacctca acatggtgct cctttgattt tgcgcaagct 1560 gtagcgcggatatattgatt ggttatctac tgtag 1595 230 517 PRT NICOTIANATABACUM 230 MetGlu Thr Leu Phe Asn Ile Lys Val Ala Val Ser Leu Val Ile Val 1 5 10 15Ile Ile Phe Leu Arg Trp Val Trp Lys Phe Leu Asn Trp Val Trp Ile 20 25 30Gln Pro Lys Lys Met Glu Lys Arg Leu Lys Met Glu Gly Phe Lys Gly 35 40 45Ser Ser Tyr Lys Leu Leu Phe Gly Asp Met Lys Glu Ile Asn Thr Met 50 55 60Val Glu Glu Ala Lys Thr Lys Pro Met Asn Phe Thr Asn Asp Tyr Val 65 70 7580 Ala Arg Val Leu Pro His Phe Thr Lys Leu Met Leu Gln Tyr Gly Lys 85 9095 Asn Ser Phe Met Trp Leu Gly Pro Lys Pro Thr Met Phe Ile Thr Asp 100105 110 Pro Glu Leu Ile Arg Glu Ile Leu Ser Lys Ser Tyr Ile Tyr Gln Glu115 120 125 Ile Gln Gly Asn Pro Ile Thr Lys Leu Leu Ala Gln Gly Leu ValSer 130 135 140 Tyr Glu Ala Glu Lys Trp Ala Lys His Arg Lys Ile Ile AsnPro Ala 145 150 155 160 Phe His Leu Asp Lys Leu Lys His Met Leu Pro SerPhe Tyr Leu Ser 165 170 175 Cys Cys Asp Met Leu Arg Lys Trp Glu Ser IleAla Ser Ser Glu Gly 180 185 190 Ser Glu Ile Asp Val Trp Pro Phe Leu GluThr Leu Thr Ser Asp Ala 195 200 205 Ile Ser Arg Thr Ala Phe Gly Ser AsnTyr Glu Asp Gly Arg Gln Ile 210 215 220 Phe Glu Leu Gln Lys Glu Gln AlaGlu Leu Ile Leu Gln Ala Ala Arg 225 230 235 240 Trp Leu Tyr Ile Pro GlyTrp Arg Phe Val Pro Thr Lys Arg Asn Lys 245 250 255 Arg Met Lys Gln IleAla Lys Glu Val Arg Ser Leu Val Leu Gly Ile 260 265 270 Ile Asn Lys ArgIle Arg Glu Met Lys Ala Gly Glu Ala Ala Lys Asp 275 280 285 Asp Leu LeuGly Ile Leu Leu Glu Ser Asn Phe Lys Glu Ile Gln Met 290 295 300 His GlyAsn Lys Asn Phe Gly Met Thr Ile Asp Glu Val Ile Glu Glu 305 310 315 320Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu 325 330335 Val Trp Thr Leu Ile Leu Leu Ser Lys His Val Asp Trp Gln Glu Arg 340345 350 Ala Arg Glu Glu Val His Gln Val Phe Gly Ser Asn Lys Pro Asp Tyr355 360 365 Asp Ala Leu Asn Gln Leu Lys Val Val Thr Met Ile Phe Asn GluVal 370 375 380 Leu Arg Leu Tyr Pro Pro Gly Ile Thr Ile Ser Arg Thr ValHis Glu 385 390 395 400 Asp Thr Lys Leu Gly Asn Leu Ser Leu Pro Ala GlyIle Gln Leu Val 405 410 415 Leu Pro Ala Ile Trp Leu His His Asp Asn GluIle Trp Gly Asp Asp 420 425 430 Ala Lys Glu Phe Lys Pro Glu Arg Phe SerGlu Gly Val Asn Lys Ala 435 440 445 Thr Lys Gly Lys Phe Ala Tyr Phe ProPhe Ser Trp Gly Pro Arg Ile 450 455 460 Cys Val Gly Leu Asn Phe Ala MetLeu Glu Ala Lys Met Ala Leu Ala 465 470 475 480 Leu Ile Leu Gln His TyrAla Phe Glu Leu Ser Pro Ser Tyr Ala His 485 490 495 Ala Pro His Thr IleIle Thr Leu Gln Pro Gln His Gly Ala Pro Leu 500 505 510 Ile Leu Arg LysLeu 515 231 1673 DNA NICOTIANATABACUM 231 atatgcaact gagatttgaagaataccaac taaccaaaat gcagttcttc agcttggttt 60 ccattttcct atttctatctttcctctttt tgttaaggat atggaagaac tccaatagcc 120 aaagcaaaaa gttgccaccaggtccatgga aactaccaat actaggaagt atgcttcata 180 tggttggtgg actaccacaccatgtcctta gagatttagc caaaaaatat ggaccactta 240 tgcaccttca attaggtgaagtttctgcgg ttgtggttac ttctcctgat acggcaaaag 300 aagtattaaa aactcatgacatcgcttttg cgtctaggcc tagccttttg gccccggaga 360 ttgtctgtta caataggtctgatctagcct tttgccccta tggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttggaagtgctca gtgccaagaa tgttcggaca tttagctcta 480 ttaggcggaa tgaagttcttcgtctcatta attttatccg gtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatctttttgttca caagctccat gacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaagacaaattta tacaactaat taaagaagtg atactcttag 660 caggagggtt tgatgtggctgacatattcc cttcactgaa gtttcttcat gtgctcagtg 720 gaatgaaggg taagattatgaatgcacacc ataaggtaga tgccattgtt gagaatgtca 780 tcaatgagca caagaaaaatcttgcaattg ggaaaactaa tggagcgtta ggaggtgaag 840 atttaattga tgttcttctaagacttatga atgatggagg ccttcaattt cctatcacca 900 acgacaacat caaagccataatttttgaca tgtttgctgc cgggacagag acttcatcgt 960 caacaattgt gtgggctatggtagaaatgg tgaaaaatcc agccgtattc gcgaaagctc 1020 aagcagaagt aagagaagcatttagaggaa aagaaacttt cgatgaaaat gatgtggagg 1080 agctaaacta cctaaagttagtaataaaag aaactctaag acttcatcca ccggttccac 1140 ttttgctccc aagagaatgtagggaagaga caaatataaa cggctacact attcctgtaa 1200 agaccaaagt catggttaatgtttgggctt tgggaagaga tccaaaatat tggaatgacg 1260 cagaaacttt tatgccagagagatttgagc agtgctctaa ggattttgtt ggtaataatt 1320 ttgaatatct tccatttggtggcggaagga ggatttgtcc tgggatttcg tttggcttag 1380 ctaatgctta tttgccattggctcaattac tatatcactt cgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttggacttgactg agttggttgg agtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcgactccttatc aacctcctca aaagtgattt aatggtttca 1560 agtttttatt tcctagcaaaccccactatt gtcctatctt tcttttggtg ttttcggttt 1620 tatctactct aatacatgcatcttttacca tataggaatg taccatgttg tcg 1673 232 514 PRT NICOTIANATABACUM232 Met Gln Leu Arg Phe Glu Glu Tyr Gln Leu Thr Lys Met Gln Phe Phe 1 510 15 Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 2025 30 Ile Trp Lys Asn Ser Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 3540 45 Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met Val Gly Gly Leu 5055 60 Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 6570 75 80 His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp85 90 95 Thr Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg100 105 110 Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser AspLeu 115 120 125 Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg LysIle Cys 130 135 140 Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr PheSer Ser Ile 145 150 155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn PheIle Arg Ser Ser Ser 165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg IlePhe Leu Phe Thr Ser Ser 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly GlnVal Phe Lys Glu Gln Asp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu ValIle Leu Leu Ala Gly Gly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro SerLeu Lys Phe Leu His Val Leu Ser Gly 225 230 235 240 Met Lys Gly Lys IleMet Asn Ala His His Lys Val Asp Ala Ile Val 245 250 255 Glu Asn Val IleAsn Glu His Lys Lys Asn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly AlaLeu Gly Gly Glu Asp Leu Ile Asp Val Leu Leu Arg Leu 275 280 285 Met AsnAsp Gly Gly Leu Gln Phe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 AlaIle Ile Phe Asp Met Phe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315320 Thr Ile Val Trp Ala Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325330 335 Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr340 345 350 Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu ValIle 355 360 365 Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu LeuPro Arg 370 375 380 Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr IlePro Val Lys 385 390 395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu GlyArg Asp Pro Lys Tyr 405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro GluArg Phe Glu Gln Cys Ser 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe GluTyr Leu Pro Phe Gly Gly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile SerPhe Gly Leu Ala Asn Ala Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu TyrHis Phe Asp Trp Lys Leu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser AspLeu Asp Leu Thr Glu Leu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys SerAsp Leu Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 2331601 DNA NICOTIANATABACUM 233 gaattatttc acgtgttgta ttccttgtctatgataggaa gctcgttacc tcagcgtaca 60 aaccccaaat aaaaaatgaa tttccttgtggtgttagctt ctctctttct ctttgtgttc 120 ctaatgagga taagcaaagc aaaaaagctccctccaggtc caaggaaact gcctataata 180 ggaaaccttc atcaaattgg aaaattacctcatcgttcac ttcaaaaact ttctaatgaa 240 tatggggatt tcattttctt gcaattaggttctgtaccga ctgtggttgt ctcctcagct 300 gacattgccc gagagatctt tagaactcacgaccttgttt tctcaggccg tcctgcttta 360 tatgctgcca gaaaactttc ctacaattgctacaacgttt catttgcacc ctatggtaat 420 tactggagag aggctcggaa aattctagtgttggagttgc taagtacaaa gagagtacaa 480 agtttcgagg caattcgaga cgaggaagtaagtagcttgg ttcaaattat ctgtagttcc 540 ttgagctcac ctgttaacat aagcacattagcactatcct tggcaaataa cgttgtttgt 600 cgagtggctt ttgggaaagg gagtgctgaaggaggaaatg attatgagga taggaagttt 660 aatgaaattc tatatgagac acaagaattattgggtgagt ttaacgttgc tgattatttt 720 cctcggatgg catggattaa caaaataaatgggtttgatg aacgattgga aaataatttt 780 agggaattgg ataagtttta tgacaaagtaatagaagatc atcttaattc atgtagctgg 840 atgaaacaaa gggatgatga agacgttattgatgtattgc ttcgaattca aaaggatcca 900 agccaagaaa ttcctctcaa agatgatcacattaagggcc ttcttgcgga tatattcata 960 gctggaactg atacatcatc aacaaccatagaatgggcaa tgtcagaact cataaaaaat 1020 ccaagagtct tgagaaaagc tcaagaggaagttagagaag tttctaaggg aaaacaaaag 1080 gtccaagaaa gtgatctttg caaactagattacttgaaat tggtcatcaa agaaaccttt 1140 agactacacc caccagtccc attactagtccctcgagtaa caacagccag ctgcaaaata 1200 atggaatacg aaattccagt aaatacaagagtcttcatca acgcgacagc aaatgggaca 1260 aatccaaaat actgggaaaa tccattgacattcttgccag agagattctt ggataaggag 1320 attgattaca gaggcaaaaa ttttgagttgttgccatttg gggcagggag aagagggtgt 1380 ccaggaatta atttttcaat accacttgttgagcttgcac ttgctaatct attgtttcat 1440 tataattggt cacttcctga agggatgctagctaaggatg ttgatatgga agaagctttg 1500 gggattacca tgcacaagaa atctcccctttgcttagtag cttctcatta tacttgttga 1560 gattttaaaa gattttagca tagctatatatagcttgaag t 1601 234 494 PRT NICOTIANATABACUM 234 Met Asn Phe Leu ValVal Leu Ala Ser Leu Phe Leu Phe Val Phe Leu 1 5 10 15 Met Arg Ile SerLys Ala Lys Lys Leu Pro Pro Gly Pro Arg Lys Leu 20 25 30 Pro Ile Ile GlyAsn Leu His Gln Ile Gly Lys Leu Pro His Arg Ser 35 40 45 Leu Gln Lys LeuSer Asn Glu Tyr Gly Asp Phe Ile Phe Leu Gln Leu 50 55 60 Gly Ser Val ProThr Val Val Val Ser Ser Ala Asp Ile Ala Arg Glu 65 70 75 80 Ile Phe ArgThr His Asp Leu Val Phe Ser Gly Arg Pro Ala Leu Tyr 85 90 95 Ala Ala ArgLys Leu Ser Tyr Asn Cys Tyr Asn Val Ser Phe Ala Pro 100 105 110 Tyr GlyAsn Tyr Trp Arg Glu Ala Arg Lys Ile Leu Val Leu Glu Leu 115 120 125 LeuSer Thr Lys Arg Val Gln Ser Phe Glu Ala Ile Arg Asp Glu Glu 130 135 140Val Ser Ser Leu Val Gln Ile Ile Cys Ser Ser Leu Ser Ser Pro Val 145 150155 160 Asn Ile Ser Thr Leu Ala Leu Ser Leu Ala Asn Asn Val Val Cys Arg165 170 175 Val Ala Phe Gly Lys Gly Ser Ala Glu Gly Gly Asn Asp Tyr GluAsp 180 185 190 Arg Lys Phe Asn Glu Ile Leu Tyr Glu Thr Gln Glu Leu LeuGly Glu 195 200 205 Phe Asn Val Ala Asp Tyr Phe Pro Arg Met Ala Trp IleAsn Lys Ile 210 215 220 Asn Gly Phe Asp Glu Arg Leu Glu Asn Asn Phe ArgGlu Leu Asp Lys 225 230 235 240 Phe Tyr Asp Lys Val Ile Glu Asp His LeuAsn Ser Cys Ser Trp Met 245 250 255 Lys Gln Arg Asp Asp Glu Asp Val IleAsp Val Leu Leu Arg Ile Gln 260 265 270 Lys Asp Pro Ser Gln Glu Ile ProLeu Lys Asp Asp His Ile Lys Gly 275 280 285 Leu Leu Ala Asp Ile Phe IleAla Gly Thr Asp Thr Ser Ser Thr Thr 290 295 300 Ile Glu Trp Ala Met SerGlu Leu Ile Lys Asn Pro Arg Val Leu Arg 305 310 315 320 Lys Ala Gln GluGlu Val Arg Glu Val Ser Lys Gly Lys Gln Lys Val 325 330 335 Gln Glu SerAsp Leu Cys Lys Leu Asp Tyr Leu Lys Leu Val Ile Lys 340 345 350 Glu ThrPhe Arg Leu His Pro Pro Val Pro Leu Leu Val Pro Arg Val 355 360 365 ThrThr Ala Ser Cys Lys Ile Met Glu Tyr Glu Ile Pro Val Asn Thr 370 375 380Arg Val Phe Ile Asn Ala Thr Ala Asn Gly Thr Asn Pro Lys Tyr Trp 385 390395 400 Glu Asn Pro Leu Thr Phe Leu Pro Glu Arg Phe Leu Asp Lys Glu Ile405 410 415 Asp Tyr Arg Gly Lys Asn Phe Glu Leu Leu Pro Phe Gly Ala GlyArg 420 425 430 Arg Gly Cys Pro Gly Ile Asn Phe Ser Ile Pro Leu Val GluLeu Ala 435 440 445 Leu Ala Asn Leu Leu Phe His Tyr Asn Trp Ser Leu ProGlu Gly Met 450 455 460 Leu Ala Lys Asp Val Asp Met Glu Glu Ala Leu GlyIle Thr Met His 465 470 475 480 Lys Lys Ser Pro Leu Cys Leu Val Ala SerHis Tyr Thr Cys 485 490 235 1570 DNA NICOTIANATABACUM 235 caaagactaaaagatgtcgg tctttgcggt tatttcattc tttctacttc tgttttttct 60 tttcaaatcatatttgccct catcgaaaac aaagaaaaat tctccaccat ctccttcaaa 120 gcttccgttaatcggtcact tccacaaact aggcttacaa cctcaccgtt ctctacaaaa 180 actatcaaatgaacatggtc ccatgatgat gcttcaattc ggtagcgtac ctgtgcttat 240 cgcttcatcagctgaagctg cttccgaaat catgaaaacc caagatttgt cttttgcaaa 300 caaacccatttcaaccattc ctagcaagct tttcttcggc ccaaaggacg ttgccttcac 360 cccatatggggattactgga ggaatgccag aagcatttgc atgcttcagc ttttgaacaa 420 caaaagagtccagtcttttc gaaagataag ggaagaagag acttctcttc ttctccagag 480 gattagggaatcgccaaatt cagaagtcga tttaacggag ctgttcgttt ccatgactaa 540 cgacatagtttgcagggtgg ccttaggaag gaagtattgt gatggggaag aagggaggaa 600 attcaagtctttgctgttag agtttgtgga attgttggga gtttttaaca ttggagatta 660 catgccgtggcttgcatgga tgaatcgttt caatggtttg aatgccaaag tggataaagt 720 ggcgaaagagtttgatgcat ttttggagga tgtgattgag gaacacggag gaaataagaa 780 atcagacactgaagctgaag gggcagactt cgtggatata ttattgcagg ttcacaaaga 840 aaacaaggctggttttcaag tcgaaatgga tgcaatcaaa gctattatca tggatatgtt 900 tgctgcgggaacagatacaa cttccacgct tctagagtgg acaatgaacg agctcttaag 960 aaatccaaaaacattgaata agttgagaga tgaggtgaga caagtgactc aagggaagac 1020 agaggtaacagaggatgact tagagaaaat gccgtattta agagcagcag ttaaggagag 1080 ttccaggctacactctccag tgccacttct acctcgagaa gcaattaagg atgcaaaggt 1140 tttgggctacgatatagctg cagggactca agtcctcgtt tgtccatggg caatctcaag 1200 agatccaaacctttgggaaa atccagagga gtttcaacct gaaagattct tggatacttc 1260 catagattacaaaggcttac atttcgagtt aattccattc ggtgcaggtc ggaggggttg 1320 ccctggcatcacatttgcta agtttgtgaa tgagctagca ttggcaagat taatgttcca 1380 ttttgatttctcgctaccaa aaggagttaa gcatgaggat ttggacgtgg aggaagctgc 1440 tggaattactgttagaagga agttccccct tttagccgtc gccactccat gctcgtgatt 1500 tttattttagagctcattct atgccttaaa aactactact agataactgc gtagtaaata 1560 atgcttggta1570 236 494 PRT NICOTIANATABACUM 236 Met Ser Val Phe Ala Val Ile SerPhe Phe Leu Leu Leu Phe Phe Leu 1 5 10 15 Phe Lys Ser Tyr Leu Pro SerSer Lys Thr Lys Lys Asn Ser Pro Pro 20 25 30 Ser Pro Ser Lys Leu Pro LeuIle Gly His Phe His Lys Leu Gly Leu 35 40 45 Gln Pro His Arg Ser Leu GlnLys Leu Ser Asn Glu His Gly Pro Met 50 55 60 Met Met Leu Gln Phe Gly SerVal Pro Val Leu Ile Ala Ser Ser Ala 65 70 75 80 Glu Ala Ala Ser Glu IleMet Lys Thr Gln Asp Leu Ser Phe Ala Asn 85 90 95 Lys Pro Ile Ser Thr IlePro Ser Lys Leu Phe Phe Gly Pro Lys Asp 100 105 110 Val Ala Phe Thr ProTyr Gly Asp Tyr Trp Arg Asn Ala Arg Ser Ile 115 120 125 Cys Met Leu GlnLeu Leu Asn Asn Lys Arg Val Gln Ser Phe Arg Lys 130 135 140 Ile Arg GluGlu Glu Thr Ser Leu Leu Leu Gln Arg Ile Arg Glu Ser 145 150 155 160 ProAsn Ser Glu Val Asp Leu Thr Glu Leu Phe Val Ser Met Thr Asn 165 170 175Asp Ile Val Cys Arg Val Ala Leu Gly Arg Lys Tyr Cys Asp Gly Glu 180 185190 Glu Gly Arg Lys Phe Lys Ser Leu Leu Leu Glu Phe Val Glu Leu Leu 195200 205 Gly Val Phe Asn Ile Gly Asp Tyr Met Pro Trp Leu Ala Trp Met Asn210 215 220 Arg Phe Asn Gly Leu Asn Ala Lys Val Asp Lys Val Ala Lys GluPhe 225 230 235 240 Asp Ala Phe Leu Glu Asp Val Ile Glu Glu His Gly GlyAsn Lys Lys 245 250 255 Ser Asp Thr Glu Ala Glu Gly Ala Asp Phe Val AspIle Leu Leu Gln 260 265 270 Val His Lys Glu Asn Lys Ala Gly Phe Gln ValGlu Met Asp Ala Ile 275 280 285 Lys Ala Ile Ile Met Asp Met Phe Ala AlaGly Thr Asp Thr Thr Ser 290 295 300 Thr Leu Leu Glu Trp Thr Met Asn GluLeu Leu Arg Asn Pro Lys Thr 305 310 315 320 Leu Asn Lys Leu Arg Asp GluVal Arg Gln Val Thr Gln Gly Lys Thr 325 330 335 Glu Val Thr Glu Asp AspLeu Glu Lys Met Pro Tyr Leu Arg Ala Ala 340 345 350 Val Lys Glu Ser SerArg Leu His Ser Pro Val Pro Leu Leu Pro Arg 355 360 365 Glu Ala Ile LysAsp Ala Lys Val Leu Gly Tyr Asp Ile Ala Ala Gly 370 375 380 Thr Gln ValLeu Val Cys Pro Trp Ala Ile Ser Arg Asp Pro Asn Leu 385 390 395 400 TrpGlu Asn Pro Glu Glu Phe Gln Pro Glu Arg Phe Leu Asp Thr Ser 405 410 415Ile Asp Tyr Lys Gly Leu His Phe Glu Leu Ile Pro Phe Gly Ala Gly 420 425430 Arg Arg Gly Cys Pro Gly Ile Thr Phe Ala Lys Phe Val Asn Glu Leu 435440 445 Ala Leu Ala Arg Leu Met Phe His Phe Asp Phe Ser Leu Pro Lys Gly450 455 460 Val Lys His Glu Asp Leu Asp Val Glu Glu Ala Ala Gly Ile ThrVal 465 470 475 480 Arg Arg Lys Phe Pro Leu Leu Ala Val Ala Thr Pro CysSer 485 490 237 1585 DNA NICOTIANATABACUM 237 attatccatc acctaaaatggagaattctt gggtttttct agccttggca gggctatctg 60 cattagcttt tctctgtaaaataatcacct gtcgaagacc ggttaaccgg aaaataccac 120 caggtccaaa accatggcccatcattggca atttgaacct acttggtcct ataccacatc 180 aatcttttga cttgctttccaaaaaatatg gagagttgat gctgctgaaa tttggctcca 240 ggccagttct tgttgcttcatctgctgaaa tggcaaaaca gtttttaaaa gtacatgatg 300 ctaatttcgc ctcccgtcctatgctagctg gtggaaagta tacaagctat aactattgtg 360 acatgacatg ggcaccctatggtccctatt ggcgccaagc acgacgaatt taccttaacc 420 agatatttac tccgaaaaggctagactcgt tcgagtacat tcgtgttgaa gaaaggcagg 480 ccttgatttc ccagctgaattcccttgctg gaaagccatt ttttctcaaa gaccatttgt 540 cgcgatttag cctctgcagcatgacaagga tggttttgag caacaagtac tttggtgaat 600 caacagttag agtagaagatttgcagtacc tggtagatca atggttctta cttaatggtg 660 ctttcaacat tggagattggattccatggc tcagcttctt ggacctacaa ggctatgtga 720 aacaaatgaa ggctttgaaaagaacttttg ataagttcca caacattgtg ctagatgatc 780 gcagggctaa gaagaatgcagagaagaact ttgtcccaaa agacatggtt gatgtcttgt 840 tgaagatggc tgaagatcctaatctggaag tcaaactcac taatgactgt gtcaaagggt 900 taatgcagga tttactaactggaggaacag atagcttaac agcagcagtg caatgggcat 960 ttcaagaact tcttagacggccaagggtta ttgagaaggc aaccgaagag cttgaccgga 1020 ttgtcgggaa agagagatgggtagaagaga aagattgctc gcagctatct tacgttgaag 1080 caatcctcaa ggaaacactaaggttacatc ctctaggaac tatgctagca ccgcattgtg 1140 ctatagaaga ttgtaacgtggctggttatg acatacagaa aggaacgacc gttctggtga 1200 atgtttggac cattggaagggacccaaaat actgggatag agcacaagag tttctccccg 1260 agagattctt agagaacgacattgatatgg acggacataa ctttgctttc ttgccatttg 1320 gctcggggcg aaggaggtgccctggctata gccttggact taaggttatc cgagtaacat 1380 tagccaacat gttgcatggattcaactgga aattacctga aggtatgaag ccagaagata 1440 taagtgtgga agaacattatgggctcacta cacatcctaa gtttcctgtt cctgtgatct 1500 tggaatctag actttcttcagatctctatt cccccatcac ttaatcctaa gtgcttccta 1560 ttatagcatc atatcaatatccctc 1585 238 508 PRT NICOTIANATABACUM 238 Met Glu Asn Ser Trp Val PheLeu Ala Leu Ala Gly Leu Ser Ala Leu 1 5 10 15 Ala Phe Leu Cys Lys IleIle Thr Cys Arg Arg Pro Val Asn Arg Lys 20 25 30 Ile Pro Pro Gly Pro LysPro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Leu Gly Pro Ile Pro HisGln Ser Phe Asp Leu Leu Ser Lys Lys Tyr 50 55 60 Gly Glu Leu Met Leu LeuLys Phe Gly Ser Arg Pro Val Leu Val Ala 65 70 75 80 Ser Ser Ala Glu MetAla Lys Gln Phe Leu Lys Val His Asp Ala Asn 85 90 95 Phe Ala Ser Arg ProMet Leu Ala Gly Gly Lys Tyr Thr Ser Tyr Asn 100 105 110 Tyr Cys Asp MetThr Trp Ala Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg IleTyr Leu Asn Gln Ile Phe Thr Pro Lys Arg Leu Asp Ser 130 135 140 Phe GluTyr Ile Arg Val Glu Glu Arg Gln Ala Leu Ile Ser Gln Leu 145 150 155 160Asn Ser Leu Ala Gly Lys Pro Phe Phe Leu Lys Asp His Leu Ser Arg 165 170175 Phe Ser Leu Cys Ser Met Thr Arg Met Val Leu Ser Asn Lys Tyr Phe 180185 190 Gly Glu Ser Thr Val Arg Val Glu Asp Leu Gln Tyr Leu Val Asp Gln195 200 205 Trp Phe Leu Leu Asn Gly Ala Phe Asn Ile Gly Asp Trp Ile ProTrp 210 215 220 Leu Ser Phe Leu Asp Leu Gln Gly Tyr Val Lys Gln Met LysAla Leu 225 230 235 240 Lys Arg Thr Phe Asp Lys Phe His Asn Ile Val LeuAsp Asp Arg Arg 245 250 255 Ala Lys Lys Asn Ala Glu Lys Asn Phe Val ProLys Asp Met Val Asp 260 265 270 Val Leu Leu Lys Met Ala Glu Asp Pro AsnLeu Glu Val Lys Leu Thr 275 280 285 Asn Asp Cys Val Lys Gly Leu Met GlnAsp Leu Leu Thr Gly Gly Thr 290 295 300 Asp Ser Leu Thr Ala Ala Val GlnTrp Ala Phe Gln Glu Leu Leu Arg 305 310 315 320 Arg Pro Arg Val Ile GluLys Ala Thr Glu Glu Leu Asp Arg Ile Val 325 330 335 Gly Lys Glu Arg TrpVal Glu Glu Lys Asp Cys Ser Gln Leu Ser Tyr 340 345 350 Val Glu Ala IleLeu Lys Glu Thr Leu Arg Leu His Pro Leu Gly Thr 355 360 365 Met Leu AlaPro His Cys Ala Ile Glu Asp Cys Asn Val Ala Gly Tyr 370 375 380 Asp IleGln Lys Gly Thr Thr Val Leu Val Asn Val Trp Thr Ile Gly 385 390 395 400Arg Asp Pro Lys Tyr Trp Asp Arg Ala Gln Glu Phe Leu Pro Glu Arg 405 410415 Phe Leu Glu Asn Asp Ile Asp Met Asp Gly His Asn Phe Ala Phe Leu 420425 430 Pro Phe Gly Ser Gly Arg Arg Arg Cys Pro Gly Tyr Ser Leu Gly Leu435 440 445 Lys Val Ile Arg Val Thr Leu Ala Asn Met Leu His Gly Phe AsnTrp 450 455 460 Lys Leu Pro Glu Gly Met Lys Pro Glu Asp Ile Ser Val GluGlu His 465 470 475 480 Tyr Gly Leu Thr Thr His Pro Lys Phe Pro Val ProVal Ile Leu Glu 485 490 495 Ser Arg Leu Ser Ser Asp Leu Tyr Ser Pro IleThr 500 505 239 1591 DNA NICOTIANATABACUM 239 ctcattatcc atcacctaaaatggagaatt cttgggtttt tctagccttg gcagggctat 60 ctgcattagc ttttctctgtaaaataatca cctgtcgaag accggttaac cggaaaatac 120 caccaggtcc aaaaccatggcccatcattg gcaatttgaa cctacttggt cctataccac 180 atcaatcttt tgacttgctttccaaaaaat atggagagtt gatgctgctg aaatttggct 240 ccaggccagt tcttgttgcttcatctgctg aaatggcaaa acagttttta aaagtacatg 300 atgctaattt cgcctcccgtcctatgctag ctggtggaaa gtatacaagc tataactatt 360 gtgacatgac atgggcaccctatggtccct attggcgcca agcacgacga cgaatttacc 420 ttaaccagat atttactccgaaaaggctag actcgttcga gtacattcgt gttgaagaaa 480 ggcaggcctt gatttcccagctgaattccc ttgctggaaa gccatttttt ctcaaagacc 540 atttgtcgcg atttagcctctgcagcatga caaggatggt tttgagcaac aagtattttg 600 gtgaatcaac agttagagtagaagatttgc agtacctggt agatcaatgg ttcttactta 660 atggtgcttt caacattggagattggattc catggctcag cttcttggac ctacaaggct 720 atgtgaaaca aatgaaggctttgaaaagaa cttttgataa gttccacaac attgtgctag 780 atgatcacag ggctaagaagaatgcagaga agaactttgt cccaaaagac atggttgatg 840 tcttgttgaa gatggctgaagatcctaatc tggaagtcaa actcactaat gactgtgtca 900 aagggttaat gcaggatttactaactggag gaacagatag cttaacagca gcagtgcaat 960 gggcatttca agaacttcttagacagccaa gggttattga gaaggcaacc gaagagcttg 1020 accggattgt cgggaaagagagatgggtag aagagaaaga ttgctcgcag ctatcttacg 1080 ttgaagcaat cctcaaggaaacactaaggt tacatcctct aggaactatg ctagcaccgc 1140 attgtgctat agaagattgtaacgtggctg gttatgacat acagaaagga acgaccgttc 1200 tggtgaatgt ttggaccattggaagggacc caaaatactg ggatagagca caagagtttc 1260 tccccgagag attcttagagaacgacattg atatggacgg acataacttt gctttcttgc 1320 catttggctc ggggcgaaggaggtgccctg gctatagcct tggacttaag gttatccgag 1380 taacattagc caacatgttgcatggattca actggaaatt acctgaaggt atgaagccag 1440 aagatataag tgtggaagaacattatgggc tcactacaca tcctaagttt cctgttcctg 1500 tgatcttgga atctagactttcttcagatc tctattcccc catcacttaa tcctaagtgc 1560 ttcctattat agcatcatatcaatatccct c 1591 240 509 PRT NICOTIANATABACUM 240 Met Glu Asn Ser TrpVal Phe Leu Ala Leu Ala Gly Leu Ser Ala Leu 1 5 10 15 Ala Phe Leu CysLys Ile Ile Thr Cys Arg Arg Pro Val Asn Arg Lys 20 25 30 Ile Pro Pro GlyPro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Leu Gly Pro IlePro His Gln Ser Phe Asp Leu Leu Ser Lys Lys Tyr 50 55 60 Gly Glu Leu MetLeu Leu Lys Phe Gly Ser Arg Pro Val Leu Val Ala 65 70 75 80 Ser Ser AlaGlu Met Ala Lys Gln Phe Leu Lys Val His Asp Ala Asn 85 90 95 Phe Ala SerArg Pro Met Leu Ala Gly Gly Lys Tyr Thr Ser Tyr Asn 100 105 110 Tyr CysAsp Met Thr Trp Ala Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 ArgArg Arg Ile Tyr Leu Asn Gln Ile Phe Thr Pro Lys Arg Leu Asp 130 135 140Ser Phe Glu Tyr Ile Arg Val Glu Glu Arg Gln Ala Leu Ile Ser Gln 145 150155 160 Leu Asn Ser Leu Ala Gly Lys Pro Phe Phe Leu Lys Asp His Leu Ser165 170 175 Arg Phe Ser Leu Cys Ser Met Thr Arg Met Val Leu Ser Asn LysTyr 180 185 190 Phe Gly Glu Ser Thr Val Arg Val Glu Asp Leu Gln Tyr LeuVal Asp 195 200 205 Gln Trp Phe Leu Leu Asn Gly Ala Phe Asn Ile Gly AspTrp Ile Pro 210 215 220 Trp Leu Ser Phe Leu Asp Leu Gln Gly Tyr Val LysGln Met Lys Ala 225 230 235 240 Leu Lys Arg Thr Phe Asp Lys Phe His AsnIle Val Leu Asp Asp His 245 250 255 Arg Ala Lys Lys Asn Ala Glu Lys AsnPhe Val Pro Lys Asp Met Val 260 265 270 Asp Val Leu Leu Lys Met Ala GluAsp Pro Asn Leu Glu Val Lys Leu 275 280 285 Thr Asn Asp Cys Val Lys GlyLeu Met Gln Asp Leu Leu Thr Gly Gly 290 295 300 Thr Asp Ser Leu Thr AlaAla Val Gln Trp Ala Phe Gln Glu Leu Leu 305 310 315 320 Arg Gln Pro ArgVal Ile Glu Lys Ala Thr Glu Glu Leu Asp Arg Ile 325 330 335 Val Gly LysGlu Arg Trp Val Glu Glu Lys Asp Cys Ser Gln Leu Ser 340 345 350 Tyr ValGlu Ala Ile Leu Lys Glu Thr Leu Arg Leu His Pro Leu Gly 355 360 365 ThrMet Leu Ala Pro His Cys Ala Ile Glu Asp Cys Asn Val Ala Gly 370 375 380Tyr Asp Ile Gln Lys Gly Thr Thr Val Leu Val Asn Val Trp Thr Ile 385 390395 400 Gly Arg Asp Pro Lys Tyr Trp Asp Arg Ala Gln Glu Phe Leu Pro Glu405 410 415 Arg Phe Leu Glu Asn Asp Ile Asp Met Asp Gly His Asn Phe AlaPhe 420 425 430 Leu Pro Phe Gly Ser Gly Arg Arg Arg Cys Pro Gly Tyr SerLeu Gly 435 440 445 Leu Lys Val Ile Arg Val Thr Leu Ala Asn Met Leu HisGly Phe Asn 450 455 460 Trp Lys Leu Pro Glu Gly Met Lys Pro Glu Asp IleSer Val Glu Glu 465 470 475 480 His Tyr Gly Leu Thr Thr His Pro Lys PhePro Val Pro Val Ile Leu 485 490 495 Glu Ser Arg Leu Ser Ser Asp Leu TyrSer Pro Ile Thr 500 505 241 1615 DNA NICOTIANATABACUM 241 tgataatgctctttctactc tttgtagccc ttcctttcat tcttattttt cttcttccta 60 aattcaaaaatggtggaaat aacagattgc caccaggtcc tataggttta ccattcattg 120 gaaatttgcatcaatacgat agtataactc ctcatatcta tttttggaaa ctttcaaaaa 180 aatatggcaaaatcttctca ttaaaacttg cttctactaa tgtggtagta gtttcttcag 240 caaaattagcaaaagaagta ttgaaaaaac aagatttaat attttgtagt agaccatcta 300 ttcttggccaacaaaaactg tcttattatg gtcgtgatat tgcttttaat gattattgga 360 gagaaatgagaaaaatttgt gttcttcatc tttttagttt aaaaaaagtt caattattta 420 gtccaattcgtgaagatgaa gtttttagaa tgattaagaa aatatcaaaa caagcttcta 480 cttcacaaattattaatttg agtaatttaa tgatttcatt aacaagtaca attatttgta 540 gagttgcttttggtgttagg attgaagaag aagcacatgc aaggaagaga tttgattttc 600 ttttggccgaggcacaagaa atgatggcta gtttctttgt atctgatttt tttccctttt 660 taagttggattgataaatta agtggattga catatagact tgagaggaat ttcaaggatt 720 tggataatttttatgaagaa ctcattgagc aacatcaaaa tcctaataag ccaaaatata 780 tggaaggagatattgttgat cttttgctac aattgaagaa agagaaatta acaccacttg 840 atctcactatggaagatata aaaggaattc tcatgaatgt gttagttgca ggatcagaca 900 ctagtgcagctgctactgtt tgggcaatga cagccttgat aaagaatcct aaagccatgg 960 aaaaagttcaattagaaatc agaaaatcag ttgggaagaa aggcattgta aatgaagaag 1020 atgtccaaaacatcccttat tttaaagcag tgataaagga aatatttaga ttgtatccac 1080 cagctccacttttagttcca agagaatcaa tggaaaaaac catattagaa ggttatgaaa 1140 ttcggccaagaaccatagtt catgttaacg cttgggctat agcaagggat cctgaaatat 1200 gggaaaatccagatgaattt atacctgaga gatttttgaa tagcagtatc gattacaagg 1260 gtcaagattttgagttactt ccatttggtg caggcagaag aggttgccca ggtattgcac 1320 ttggggttgcatccatggaa cttgctttgt caaatcttct ttatgcattt gattgggagt 1380 tgccttatggagtaaaaaaa gaagacatcg acacaaacgt taggcctgga attgccatgc 1440 acaagaaaaacgaactttgc cttgtcccaa aaaattattt ataaattata ttgggacgtg 1500 gatctcatgctagttctgtg cggtcagcta agcttattat ttttggctca aattatgtat 1560 acataattagtacatgttta aaatgtataa atatagtaga accattctca tggtt 1615 242 492 PRTNICOTIANATABACUM 242 Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile LeuIle Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg LeuPro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln TyrAsp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys TyrGly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val ValSer Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp LeuIle Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser TyrTyr Gly Arg Asp Ile 100 105 110 Ala Phe Asn Asp Tyr Trp Arg Glu Met ArgLys Ile Cys Val Leu His 115 120 125 Leu Phe Ser Leu Lys Lys Val Gln LeuPhe Ser Pro Ile Arg Glu Asp 130 135 140 Glu Val Phe Arg Met Ile Lys LysIle Ser Lys Gln Ala Ser Thr Ser 145 150 155 160 Gln Ile Ile Asn Leu SerAsn Leu Met Ile Ser Leu Thr Ser Thr Ile 165 170 175 Ile Cys Arg Val AlaPhe Gly Val Arg Ile Glu Glu Glu Ala His Ala 180 185 190 Arg Lys Arg PheAsp Phe Leu Leu Ala Glu Ala Gln Glu Met Met Ala 195 200 205 Ser Phe PheVal Ser Asp Phe Phe Pro Phe Leu Ser Trp Ile Asp Lys 210 215 220 Leu SerGly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys Asp Leu Asp 225 230 235 240Asn Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro Asn Lys Pro 245 250255 Lys Tyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln Leu Lys Lys 260265 270 Glu Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile Lys Gly Ile275 280 285 Leu Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala Ala AlaThr 290 295 300 Val Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala MetGlu Lys 305 310 315 320 Val Gln Leu Glu Ile Arg Lys Ser Val Gly Lys LysGly Ile Val Asn 325 330 335 Glu Glu Asp Val Gln Asn Ile Pro Tyr Phe LysAla Val Ile Lys Glu 340 345 350 Ile Phe Arg Leu Tyr Pro Pro Ala Pro LeuLeu Val Pro Arg Glu Ser 355 360 365 Met Glu Lys Thr Ile Leu Glu Gly TyrGlu Ile Arg Pro Arg Thr Ile 370 375 380 Val His Val Asn Ala Trp Ala IleAla Arg Asp Pro Glu Ile Trp Glu 385 390 395 400 Asn Pro Asp Glu Phe IlePro Glu Arg Phe Leu Asn Ser Ser Ile Asp 405 410 415 Tyr Lys Gly Gln AspPhe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg 420 425 430 Gly Cys Pro GlyIle Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu 435 440 445 Ser Asn LeuLeu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val Lys 450 455 460 Lys GluAsp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala Met His Lys 465 470 475 480Lys Asn Glu Leu Cys Leu Val Pro Lys Asn Tyr Leu 485 490 243 1624 DNANICOTIANATABACUM 243 tgataatgct ctttctactc tttgtagccc ttcctttcattcttattttt cttcttccta 60 aattcaaaaa tggtggaaat aacagattgc caccaggtcctataggttta ccattcattg 120 gaaatttgca tcaatatgat agtataactc ctcatatctatttttggaaa ctttccaaaa 180 aatatggcaa aatcttctca ttaaaacttg cttctactaatgtggtagta gtttcttcag 240 caaaattagc aaaagaagta ttgaaaaaac aagatttaatattttgtagt agaccatcta 300 ttcttggcca acaaaaactg tcttattatg gtcgtgatattgcttttgca ccttataatg 360 attattggag agaaatgaga aaaatttgtg ttcttcatctttttagttta aaaaaagttc 420 aattatttag tccaattcgt gaagatgaag tttttagaatgattaagaaa atatcaaaac 480 aagcttctac ttcacaaatt attaatttga gtaatttaatgatttcatta acaagtacaa 540 ttatttgtag agttgctttt ggtgttaggt ttgaagaagaagcacatgca aggaagagat 600 ttgattttct tttggccgag gcacaagaaa tgatggctagtttctttgta tctgattttt 660 ttcccttttt aagttggatt gataaattaa gtggattgacatatagactt gagaggaatt 720 tcaaggattt ggataatttt tatgaagaac tcattgagcaacatcaaaat cctaataagc 780 caaaatatat ggaaggagat attgttgatc ttttgctacaattgaagaaa gagaaattaa 840 caccacttga tctcactatg gaagatataa aaggaattctcatgaatgtg ttagttgcag 900 gatcagacac tagtgcagct gctactgttt gggcaatgacagccttgata aagaatccta 960 aagccatgga aaaagttcaa ttagaaatca gaaaatcagttgggaagaaa ggcattgtaa 1020 atgaagaaga tgtccaaaac atcccttatt ttaaagcagtgataaaggaa atatttagat 1080 tgtatccacc agctccactt ttagttccaa gagaatcaatggaaaaaacc atattagaag 1140 gttatgaaat tcggccaaga accatagttc atgttaacgcttgggctata gcaagggatc 1200 ctgaaatatg ggaaaatcca gatgaattta tacctgagagatttttgaat agcagtatcg 1260 attacaaggg tcaagatttt gagttacttc catttggtgcaggcagaaga ggttgcccag 1320 gtattgcact tggggttgca tccatggaac ttgctttgtcaaatcttctt tatgcatttg 1380 attgggagtt gccttatgga gtgaaaaaag aagacatcgacacaaacgtt aggcctggaa 1440 ttgccatgca caagaaaaac gaactttgcc ttgtcccaaaaaattattta taaattatat 1500 tgggacgtgg atctcatgct agttctgtgc ggtcagctaagcttattatt tttggctcaa 1560 attatgtata cataattagt acatgtttaa aatgtataaatatagtagaa ccattctcat 1620 ggtt 1624 244 495 PRT NICOTIANATABACUM 244Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile Leu Ile Phe Leu 1 5 1015 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 2530 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 4045 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 5560 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val Val Ser Ser Ala Lys 65 7075 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 8590 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile100 105 110 Ala Phe Ala Pro Tyr Asn Asp Tyr Trp Arg Glu Met Arg Lys IleCys 115 120 125 Val Leu His Leu Phe Ser Leu Lys Lys Val Gln Leu Phe SerPro Ile 130 135 140 Arg Glu Asp Glu Val Phe Arg Met Ile Lys Lys Ile SerLys Gln Ala 145 150 155 160 Ser Thr Ser Gln Ile Ile Asn Leu Ser Asn LeuMet Ile Ser Leu Thr 165 170 175 Ser Thr Ile Ile Cys Arg Val Ala Phe GlyVal Arg Phe Glu Glu Glu 180 185 190 Ala His Ala Arg Lys Arg Phe Asp PheLeu Leu Ala Glu Ala Gln Glu 195 200 205 Met Met Ala Ser Phe Phe Val SerAsp Phe Phe Pro Phe Leu Ser Trp 210 215 220 Ile Asp Lys Leu Ser Gly LeuThr Tyr Arg Leu Glu Arg Asn Phe Lys 225 230 235 240 Asp Leu Asp Asn PheTyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro 245 250 255 Asn Lys Pro LysTyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln 260 265 270 Leu Lys LysGlu Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile 275 280 285 Lys GlyIle Leu Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala 290 295 300 AlaAla Thr Val Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala 305 310 315320 Met Glu Lys Val Gln Leu Glu Ile Arg Lys Ser Val Gly Lys Lys Gly 325330 335 Ile Val Asn Glu Glu Asp Val Gln Asn Ile Pro Tyr Phe Lys Ala Val340 345 350 Ile Lys Glu Ile Phe Arg Leu Tyr Pro Pro Ala Pro Leu Leu ValPro 355 360 365 Arg Glu Ser Met Glu Lys Thr Ile Leu Glu Gly Tyr Glu IleArg Pro 370 375 380 Arg Thr Ile Val His Val Asn Ala Trp Ala Ile Ala ArgAsp Pro Glu 385 390 395 400 Ile Trp Glu Asn Pro Asp Glu Phe Ile Pro GluArg Phe Leu Asn Ser 405 410 415 Ser Ile Asp Tyr Lys Gly Gln Asp Phe GluLeu Leu Pro Phe Gly Ala 420 425 430 Gly Arg Arg Gly Cys Pro Gly Ile AlaLeu Gly Val Ala Ser Met Glu 435 440 445 Leu Ala Leu Ser Asn Leu Leu TyrAla Phe Asp Trp Glu Leu Pro Tyr 450 455 460 Gly Val Lys Lys Glu Asp IleAsp Thr Asn Val Arg Pro Gly Ile Ala 465 470 475 480 Met His Lys Lys AsnGlu Leu Cys Leu Val Pro Lys Asn Tyr Leu 485 490 495 245 1627 DNANICOTIANATABACUM 245 aaaattcata atggtttttc ccatagaagc ctttgtaggactagtaacct tcacatttct 60 cttatacttc ctatggacaa aaaaatctca aaaacttccaaaacccttac taccgaaaat 120 ccccggagga tggccggtaa tcggccatct ttttcacttcaataacgacg gcgacgaccg 180 tccattagct cgaaaactcg gagacttagc tgataaatacggccccgttt tcacttttcg 240 gctaggtctt ccccttgtgc tagttgtaag cagttacgaagctataaaag attgcttctc 300 tacaaatgac gccattttct ccaatcgtcc agcttttctttacggcgaat accttggcta 360 caataataca atgctttttc tagcaaatta cggaccttactggcgaaaaa atcgtaaatt 420 agtcattcag gaagttctct ctgctagtcg tctcgaaaaattcaaacaag tgagattcac 480 cagaattcaa acgagcatta agaatttata cactcgaattaatggaaatt cgagtacgat 540 aaatctaact gattggttag aagaattgga ttttggtctgatcgtgaaaa tgatcgctgg 600 gaaaaattat gaatccggta aaggagatga acaagtggaaagatttaaga atgcgtttaa 660 ggattttatg gttttatcaa tggaatttgt attatgggatgcatttccaa ttccattatt 720 taaatgggtg gattttcaag gtcatattaa ggcaatgaaaaggacattta aggatataga 780 ttctgttttt cagaactggt tagaggaaca tattaataaaagagaaaaaa tggaggttgg 840 tgcagaaggg aatgaacaag atttcattga tgtggtgctttcaaaattga gtaaagaata 900 tcttgatgaa ggttactctc gtgatactgt cattaaagcaacagttttta gtttggtctt 960 ggatgcagca gacacagttg ctcttcacat aaattggggaatgacattat tgataaacaa 1020 tcaaaatgcc ttgatgaaag cacaagaaga gatagacacaaaagttggta agtatagatg 1080 ggtagaagag agtgatatta aggatttagt atacctccaagctattgtta aaaaggtgtt 1140 acgattatat ccaccaggac ctttgttagt accacatgaatatgtaaagg attgtgttgt 1200 tagtggatat cacattccta aagggactag attattcgcaaacgtcatga aactgcagcg 1260 cgatcctaaa ctcttgtcaa atcctgataa gttcgatccagagagattca tcgctggtga 1320 tatcgacttc cgtggtcacc actatgagtt tatcccatttggttctggaa gacgatcttg 1380 tccggggatg acttatgcat tgcaagtgga acacctaacaatggcacatt taatccaggg 1440 tttcaattac aaaactccaa atgacgaggc cttggatatgaaggaaggtg caggcataac 1500 aatacgtaag gtaaatccgg tggaattgat aataacgcctcgcttggcac ctgagcttta 1560 ctaaaaccta agatctttca tcttggttga tcattgtttaatactcctag atagatgggt 1620 attcatc 1627 246 517 PRT NICOTIANATABACUM 246Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe 1 5 1015 Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro 20 2530 Leu Leu Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 4045 His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 5560 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 7075 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe 8590 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly100 105 110 Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn TyrGly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu ValLeu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe ThrArg Ile Gln 145 150 155 160 Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile AsnGly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu LeuAsp Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr GluSer Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Asn Ala PheLys Asp Phe Met Val Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp AlaPhe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly HisIle Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val PheGln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met GluVal Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val LeuSer Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg 290 295 300 AspThr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn 325330 335 Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val340 345 350 Gly Lys Tyr Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu ValTyr 355 360 365 Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro ProGly Pro 370 375 380 Leu Leu Val Pro His Glu Tyr Val Lys Asp Cys Val ValSer Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala AsnVal Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Leu Ser Asn Pro AspLys Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Gly Asp Ile Asp Phe ArgGly His His Tyr Glu Phe Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg SerCys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr MetAla His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Lys Thr Pro Asn AspGlu Ala Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg LysVal Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu 500 505 510 Ala Pro GluLeu Tyr 515 247 1594 DNA NICOTIANATABACUM 247 caaaaaatca tttctctcgtctaaaatgga tcttctctta ctagagaaga ccttaattgg 60 tcttttcttt gccattttaatcgctttaat tgtctctaaa cttcgttcaa agcgttttaa 120 gcttcctcca ggaccaattccagtaccagt ttttggtaat tggcttcaag ttggtgatga 180 tttaaaccac agaaatcttactgattatgc caaaaaattt ggcgatcttt tcttgttaag 240 aatgggtcaa cgtaacttagttgttgtgtc atctcctgaa ttagctaaag aagttttaca 300 cacacaaggt gttgaatttggttcaagaac aagaaatgtt gtgtttgata tttttactgg 360 aaaaggtcaa gatatggtttttactgtata tggtgaacat tggagaaaaa tgaggagaat 420 tatgactgta ccattttttactaataaagt tgtgcaacag tatagagggg ggtgggagtt 480 tgaggtggca agtgtaattgaggatgtgaa aaaaaatcct gaatctgcta ctaatgggat 540 cgtattaagg aggagattacaattaatgat gtataataat atgtttagga ttatgtttga 600 taggagattt gagagtgaagatgatccttt gtttgttaag cttaaggctt tgaatggtga 660 aaggagtaga ttggctcaaagttttgagta taattatggt gattttattc caattttgag 720 gcctcttttg agaggttatttgaagatctg taaagaagtt aaggagaaga ggctgcagct 780 tttcaaagat tactttgttgatgaaagaaa gaagctttca aataccaaga gctcggacag 840 caatgcccta aaatgtgcgattgatcacat tcttgaggct caacagaagg gagagatcaa 900 tgaggacaac gttctttacattgttgaaaa catcaatgtt gctgcaattg aaacaacatt 960 atggtcaatt gagtggggtatcgccgagct agtcaaccac cctcacatcc aaaagaaact 1020 gcgcgacgag attgacacagttcttggacc aggagtgcaa gtgactgaac cagacaccca 1080 caagcttcca taccttcaggctgtgatcaa ggaggcactt cgtctccgta tggcaattcc 1140 tctattagtc ccacacatgaaccttcacga cgcaaagctt ggcgggcttg atattccagc 1200 agagagcaaa atcttggttaacgcttggtg gttagctaac aacccggctc attggaagaa 1260 acccgaagag ttcagacccgagaggttctt tgaagaggag aagcatgttg aggccaatgg 1320 caatgacttc agatatcttccgtttggcgt tggtaggagg agctgccctg gaattatact 1380 tgcattgcca attcttggcatcactttggg acgtttggtt cagaactttg agctgttgcc 1440 tcctccaggc cagtcgaagctcgacaccac agagaaaggt ggacagttca gtctccacat 1500 tttgaagcat tccaccattgtgttgaaacc aaggtctttc tgaactttgt gatcttatta 1560 attaaggggt tctgaagaaatttgatagtg ttgg 1594 248 505 PRT NICOTIANATABACUM 248 Met Asp Leu LeuLeu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu IleAla Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro ProGly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly AspAsp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys 50 55 60 Phe Gly AspLeu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val SerSer Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu PheGly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 LysGly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu ArgArg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile MetPhe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val LysLeu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser PheGlu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Leu LeuArg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu Val Lys Glu Lys ArgLeu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp Glu Arg Lys Lys LeuSer Asn Thr Lys Ser Ser Asp Ser 260 265 270 Asn Ala Leu Lys Cys Ala IleAsp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly Glu Ile Asn Glu AspAsn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 Val Ala Ala Ile GluThr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320 Glu Leu ValAsn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330 335 Asp ThrVal Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His 340 345 350 LysLeu Pro Tyr Leu Gln Ala Val Ile Lys Glu Ala Leu Arg Leu Arg 355 360 365Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys 370 375380 Leu Gly Gly Leu Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala 385390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu GluPhe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu AlaAsn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg ArgSer Cys Pro 435 440 445 Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile ThrLeu Gly Arg Leu 450 455 460 Val Gln Asn Phe Glu Leu Leu Pro Pro Pro GlyGln Ser Lys Leu Asp 465 470 475 480 Thr Thr Glu Lys Gly Gly Gln Phe SerLeu His Ile Leu Lys His Ser 485 490 495 Thr Ile Val Leu Lys Pro Arg SerPhe 500 505 249 1678 DNA NICOTIANATABACUM 249 aacattttgc aatatagttttcctagtcag ttctagcctc cttttcctta gaaataatgg 60 attatcatat ttctttccattttcaagctc ttttagggct tttagccttt gtgttcttgt 120 ctattatctt atggagaagaacactcactt caagaaaatt agcccctgaa atcccagggg 180 catggcctat tataggccatcttcgtcagc tgagtggtac tgataagaat atcccatttc 240 cccgaatatt gggcgctttggcagataaat atggacctgt cttcacactg agaataggga 300 tgtaccccta tttgattgtcaacaattggg aagcagctaa ggattgtctc acaacgcatg 360 ataaggactt cgctgcccgaccaacttcta tggctggtga aagcatcggg tacaagtatg 420 cgaggtttac ttatgctaattttggtcctt attataacca agtgcgcaaa ctagccctac 480 aacatgtacc ctcgagtactaaactcgaga aaatgaaaca catacgtgtt tctgaattgg 540 aaactagcat caaagaattatattctttga cgctgggcaa aaacaacatg caaaaagtga 600 atataagtaa atggtttgaacaattgactt taaacataat cgtgaagaca atttgtggca 660 agagatatag caacatagaggaggatgaag aggcacaacg tttcagaaag gcatttaagg 720 gcatcatgtt tgttgtagggcaaattgttt tatatgacgc aattccattc ccattgttca 780 aatactttga tttccaaggtcatatacaat tgatgaacaa aatttataaa gacttagatt 840 ctattcttca aggatggttggatgatcata tgatgaacaa ggatgtaaac aataaggatc 900 aagatgccat agatgccatgcttaaggtaa cacaacttaa tgaattcaaa gcctatggtt 960 tttctcaggc cactgtgatcaagtcgacag tcttgagttt gatcttagat ggaaatgaca 1020 caaccgctgt tcatttgatatgggtaatgt ccttattact gaacaatcca catgttatga 1080 aacaaggcca agaagagatagacatgaaag tgggtaaaga gaggtggatt gaagatactg 1140 acataaaaaa tttagtgtaccttcaggcta tcgttaaaga gacattgcgc ttgtatccac 1200 ctgttccttt tcttttaccacacgaagcag tgcaagattg taaagtgact ggttaccaca 1260 ttcctaaagg tactcgtctatatatcaatg cgtggaaagt acatcgcgat cctgaaattt 1320 ggtcagagcc cgaaaagtttatgcccaata gattcttgac tagcaaagca aatatagatg 1380 ctcgcggtca aaattttgaatttataccgt ttggttctgg gagacggtca tgtccaggga 1440 taggttttgc gactttagtgacacatctga cttttggtcg cttgcttcaa ggttttgatt 1500 ttagtaagcc atcaaacacgccaattgaca tgacagaagg cgtaggcgtt actttgccta 1560 aggttaatca agttgaagttctaattaccc ctcgtttacc ttctaagctt tatttatttt 1620 gaaagtgcaa atcatcaatcatggcttgag taattagtta tactttaata tgtttctc 1678 250 521 PRTNICOTIANATABACUM 250 Met Asp Tyr His Ile Ser Phe His Phe Gln Ala Leu LeuGly Leu Leu 1 5 10 15 Ala Phe Val Phe Leu Ser Ile Ile Leu Trp Arg ArgThr Leu Thr Ser 20 25 30 Arg Lys Leu Ala Pro Glu Ile Pro Gly Ala Trp ProIle Ile Gly His 35 40 45 Leu Arg Gln Leu Ser Gly Thr Asp Lys Asn Ile ProPhe Pro Arg Ile 50 55 60 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val PheThr Leu Arg Ile 65 70 75 80 Gly Met Tyr Pro Tyr Leu Ile Val Asn Asn TrpGlu Ala Ala Lys Asp 85 90 95 Cys Leu Thr Thr His Asp Lys Asp Phe Ala AlaArg Pro Thr Ser Met 100 105 110 Ala Gly Glu Ser Ile Gly Tyr Lys Tyr AlaArg Phe Thr Tyr Ala Asn 115 120 125 Phe Gly Pro Tyr Tyr Asn Gln Val ArgLys Leu Ala Leu Gln His Val 130 135 140 Pro Ser Ser Thr Lys Leu Glu LysMet Lys His Ile Arg Val Ser Glu 145 150 155 160 Leu Glu Thr Ser Ile LysGlu Leu Tyr Ser Leu Thr Leu Gly Lys Asn 165 170 175 Asn Met Gln Lys ValAsn Ile Ser Lys Trp Phe Glu Gln Leu Thr Leu 180 185 190 Asn Ile Ile ValLys Thr Ile Cys Gly Lys Arg Tyr Ser Asn Ile Glu 195 200 205 Glu Asp GluGlu Ala Gln Arg Phe Arg Lys Ala Phe Lys Gly Ile Met 210 215 220 Phe ValVal Gly Gln Ile Val Leu Tyr Asp Ala Ile Pro Phe Pro Leu 225 230 235 240Phe Lys Tyr Phe Asp Phe Gln Gly His Ile Gln Leu Met Asn Lys Ile 245 250255 Tyr Lys Asp Leu Asp Ser Ile Leu Gln Gly Trp Leu Asp Asp His Met 260265 270 Met Asn Lys Asp Val Asn Asn Lys Asp Gln Asp Ala Ile Asp Ala Met275 280 285 Leu Lys Val Thr Gln Leu Asn Glu Phe Lys Ala Tyr Gly Phe SerGln 290 295 300 Ala Thr Val Ile Lys Ser Thr Val Leu Ser Leu Ile Leu AspGly Asn 305 310 315 320 Asp Thr Thr Ala Val His Leu Ile Trp Val Met SerLeu Leu Leu Asn 325 330 335 Asn Pro His Val Met Lys Gln Gly Gln Glu GluIle Asp Met Lys Val 340 345 350 Gly Lys Glu Arg Trp Ile Glu Asp Thr AspIle Lys Asn Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Thr LeuArg Leu Tyr Pro Pro Val Pro 370 375 380 Phe Leu Leu Pro His Glu Ala ValGln Asp Cys Lys Val Thr Gly Tyr 385 390 395 400 His Ile Pro Lys Gly ThrArg Leu Tyr Ile Asn Ala Trp Lys Val His 405 410 415 Arg Asp Pro Glu IleTrp Ser Glu Pro Glu Lys Phe Met Pro Asn Arg 420 425 430 Phe Leu Thr SerLys Ala Asn Ile Asp Ala Arg Gly Gln Asn Phe Glu 435 440 445 Phe Ile ProPhe Gly Ser Gly Arg Arg Ser Cys Pro Gly Ile Gly Phe 450 455 460 Ala ThrLeu Val Thr His Leu Thr Phe Gly Arg Leu Leu Gln Gly Phe 465 470 475 480Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr Glu Gly Val 485 490495 Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu Ile Thr Pro 500505 510 Arg Leu Pro Ser Lys Leu Tyr Leu Phe 515 520 251 1537 DNANICOTIANATABACUM 251 tgataatgct ctttctactc tttgtagccc ttcctttcattcttattttt cttcttccta 60 aattcaaaaa tggtggaaat aacagattgc caccaggtcctataggttta ccattcattg 120 gaaatttgca tcaatatgat agtataactc ctcatatctatttttggaaa ctttccaaaa 180 aatatggcaa aatcttctca ttaaaacttg cttctactaatgtggtagta gtttcttcag 240 caaaattagc aaaagaagta ttgaaaaaac aagatttaatattttgtagt agaccatcta 300 ttcttggcca acaaaaactg tcttattatg gtcgtgatattgcttttgca ccttataatg 360 attattggag agaaatgaga aaaatttgtg ttcttcatctttttagttta aaaaaagttc 420 aattatttag tccaattcgt gaagatgaag tttttagaatgattaagaaa atatcaaaac 480 aagcttctac ttcacaaatt attaatttga gtaatttaatgatttcatta acaagtacaa 540 ttatttgtag agttgctttt ggtgttaggt ttgaagaagaagcacatgca aggaagagat 600 ttgattttct tttggccgag gcacaagaaa tgatggctagtttctttgta tctgattttt 660 ttcccttttt aagttggatt gataaattaa gtggattgacatatagactt gagaggaatt 720 tcaaggattt ggataatttt tatgaagaac tcattgagcaacatcaaaat cctaataagc 780 caaaatatat ggaaggagat attgttgatc ttttgctacaattgaagaaa gagaaattaa 840 caccacttga tctcactatg gaagatataa aaggaattctcatgaatgtg ttagttgcag 900 gatcagacac tagtgcagct gctactgttt gggcaatgacagccttgata aagaatccta 960 aagccatgga aaaagttcaa ttagaaatca gaaaatcagttgggaagaaa ggcattgtaa 1020 atgaagaaga tgtccaaaac atcccttatt ttaaagcagtgataaaggaa atatttagat 1080 tgtatccacc agctccactt ttagttccaa gagaatcaatggaaaaaacc atattagaag 1140 gttatgaaat tcggccaaga accatagttc atgttaacgcttgggctata gcaagggatc 1200 ctgaaatatg ggaaaatcca gatgaattta tacctgagagatttttgaat agcagtaccg 1260 attacaaggg tcaagatttt gagttacttc catttggtgcaggcagaaga ggttgcccag 1320 gtattgcact tggggttgca tccatggaac ttgctttgtcaaatcttctt tatgcatttg 1380 attgggagtt gccttatgga gtgaaaaaag aagacatcgacacaaacgtt aggcctggaa 1440 ttgccatgca caagaaaaac gaactttgcc ttgtcccaaaaaattattta taaattatat 1500 tgggacgtgg atctcaattt agttctgtga ggtcagc 1537252 495 PRT NICOTIANATABACUM 252 Met Leu Phe Leu Leu Phe Val Ala Leu ProPhe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly AsnAsn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn LeuHis Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu SerLys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn ValVal Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys LysGln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln LysLeu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala Pro Tyr Asn AspTyr Trp Arg Glu Met Arg Lys Ile Cys 115 120 125 Val Leu His Leu Phe SerLeu Lys Lys Val Gln Leu Phe Ser Pro Ile 130 135 140 Arg Glu Asp Glu ValPhe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala 145 150 155 160 Ser Thr SerGln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr 165 170 175 Ser ThrIle Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu 180 185 190 AlaHis Ala Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu 195 200 205Met Met Ala Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Trp 210 215220 Ile Asp Lys Leu Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys 225230 235 240 Asp Leu Asp Asn Phe Tyr Glu Glu Leu Ile Glu Gln His Gln AsnPro 245 250 255 Asn Lys Pro Lys Tyr Met Glu Gly Asp Ile Val Asp Leu LeuLeu Gln 260 265 270 Leu Lys Lys Glu Lys Leu Thr Pro Leu Asp Leu Thr MetGlu Asp Ile 275 280 285 Lys Gly Ile Leu Met Asn Val Leu Val Ala Gly SerAsp Thr Ser Ala 290 295 300 Ala Ala Thr Val Trp Ala Met Thr Ala Leu IleLys Asn Pro Lys Ala 305 310 315 320 Met Glu Lys Val Gln Leu Glu Ile ArgLys Ser Val Gly Lys Lys Gly 325 330 335 Ile Val Asn Glu Glu Asp Val GlnAsn Ile Pro Tyr Phe Lys Ala Val 340 345 350 Ile Lys Glu Ile Phe Arg LeuTyr Pro Pro Ala Pro Leu Leu Val Pro 355 360 365 Arg Glu Ser Met Glu LysThr Ile Leu Glu Gly Tyr Glu Ile Arg Pro 370 375 380 Arg Thr Ile Val HisVal Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu 385 390 395 400 Ile Trp GluAsn Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser 405 410 415 Ser ThrAsp Tyr Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala 420 425 430 GlyArg Arg Gly Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu 435 440 445Leu Ala Leu Ser Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr 450 455460 Gly Val Lys Lys Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala 465470 475 480 Met His Lys Lys Asn Glu Leu Cys Leu Val Pro Lys Asn Tyr Leu485 490 495 253 1572 DNA NICOTIANATABACUM 253 ccaaaatcat ggctctatctttcatattca tatccataac cctaattttt ctagttcata 60 aactctacca ccgtcttagattcaaactac caccaggtcc gcggccgtta ccggtggtcg 120 gaaacctcta cgacataaaaccggtgagat tccggtgctt tgccgattgg gccaaaactt 180 acggtccgat tttctcagtatactttgggt cacagttaaa tgttgtggta acaacagctg 240 aattagctaa agaagtattgaaagaaaatg accagaattt agcagataga tttaggacta 300 gacctgcaaa taatttgagcagaaatggga tggatttgat ttgggctgat tatgggcctc 360 attatgtgaa agtaaggaagctctgtaatc ttgagctttt tactcctaaa agacttgaag 420 ctcttagacc tattagagaagatgaagtta ctgctatggt tgaaaacatt ttcaaggatt 480 gtactaagcc tgataacacaggtaaaagct tgttgataag agagtactta ggatcagtag 540 cattcaacaa cattacaaggttaacatttg ggaaaaggtt catgaactca aaaggtgaga 600 ttgatgagca aggtcaagaattcaagggta ttgtctctaa tggcatcaaa attggcggaa 660 aacttccctt ggcagagtatgttccatggc tccgttggtt tttcacaatg gaaaacgagg 720 cactcgtgaa gcactctgcacgtagagacc ggttaacaag aatgatcatg gatgaacaca 780 cactggctcg caagaaaactggtgatacta agcagcattt tgtcgatgca ttgcttactc 840 ttcagaagca gtatgatcttagtgatgaca ctgttattgg cctcctctgg gatatgatta 900 cagcaggaat ggacacaacaaccataacag tggaatgggc aatggcagaa ctagttaaga 960 acccaagagt gcaactaaaagctcaagagg agcttgacag ggtaatcgga acggatcgaa 1020 tcatgtcaga aaccgatttctctaaacttc cttacctaca atgtgtagcc aaagaggctc 1080 taaggttgca ccctccaactcctctaatgc ttcctcataa ggccagtgcc agtgtcaaaa 1140 ttggtggtta tgacattcctaaggggtcca tcgtgcacgt gaacgtttgg gctgtcgctc 1200 gtgacccagc cgtgtggaagaacccgttgg agttcagacc agagcgcttc cttgaggaag 1260 acgttgacat gaagggtcacgactatcggt tattgccctt tggtgcagga aggcgtgttt 1320 gccccggtgc acaacttgctatcaacttgg tcacatctat gttgggtcat ttgttgcatc 1380 attttacatg ggctccggccccgggggtta acccggagga tattgacttg gaggagagcc 1440 ctggaacagt aacttacatgaaaaatccaa tacaagctat tccaactcca agattgcctg 1500 cacacttgta tggacgtgtgccagtggata tgtaaaacat tttgttcttt ccctttttgg 1560 ttatatgatg ag 1572 254508 PRT NICOTIANATABACUM 254 Met Ala Leu Ser Phe Ile Phe Ile Ser Ile ThrLeu Ile Phe Leu Val 1 5 10 15 His Lys Leu Tyr His Arg Leu Arg Phe LysLeu Pro Pro Gly Pro Arg 20 25 30 Pro Leu Pro Val Val Gly Asn Leu Tyr AspIle Lys Pro Val Arg Phe 35 40 45 Arg Cys Phe Ala Asp Trp Ala Lys Thr TyrGly Pro Ile Phe Ser Val 50 55 60 Tyr Phe Gly Ser Gln Leu Asn Val Val ValThr Thr Ala Glu Leu Ala 65 70 75 80 Lys Glu Val Leu Lys Glu Asn Asp GlnAsn Leu Ala Asp Arg Phe Arg 85 90 95 Thr Arg Pro Ala Asn Asn Leu Ser ArgAsn Gly Met Asp Leu Ile Trp 100 105 110 Ala Asp Tyr Gly Pro His Tyr ValLys Val Arg Lys Leu Cys Asn Leu 115 120 125 Glu Leu Phe Thr Pro Lys ArgLeu Glu Ala Leu Arg Pro Ile Arg Glu 130 135 140 Asp Glu Val Thr Ala MetVal Glu Asn Ile Phe Lys Asp Cys Thr Lys 145 150 155 160 Pro Asp Asn ThrGly Lys Ser Leu Leu Ile Arg Glu Tyr Leu Gly Ser 165 170 175 Val Ala PheAsn Asn Ile Thr Arg Leu Thr Phe Gly Lys Arg Phe Met 180 185 190 Asn SerLys Gly Glu Ile Asp Glu Gln Gly Gln Glu Phe Lys Gly Ile 195 200 205 ValSer Asn Gly Ile Lys Ile Gly Gly Lys Leu Pro Leu Ala Glu Tyr 210 215 220Val Pro Trp Leu Arg Trp Phe Phe Thr Met Glu Asn Glu Ala Leu Val 225 230235 240 Lys His Ser Ala Arg Arg Asp Arg Leu Thr Arg Met Ile Met Asp Glu245 250 255 His Thr Leu Ala Arg Lys Lys Thr Gly Asp Thr Lys Gln His PheVal 260 265 270 Asp Ala Leu Leu Thr Leu Gln Lys Gln Tyr Asp Leu Ser AspAsp Thr 275 280 285 Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly MetAsp Thr Thr 290 295 300 Thr Ile Thr Val Glu Trp Ala Met Ala Glu Leu ValLys Asn Pro Arg 305 310 315 320 Val Gln Leu Lys Ala Gln Glu Glu Leu AspArg Val Ile Gly Thr Asp 325 330 335 Arg Ile Met Ser Glu Thr Asp Phe SerLys Leu Pro Tyr Leu Gln Cys 340 345 350 Val Ala Lys Glu Ala Leu Arg LeuHis Pro Pro Thr Pro Leu Met Leu 355 360 365 Pro His Lys Ala Ser Ala SerVal Lys Ile Gly Gly Tyr Asp Ile Pro 370 375 380 Lys Gly Ser Ile Val HisVal Asn Val Trp Ala Val Ala Arg Asp Pro 385 390 395 400 Ala Val Trp LysAsn Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Glu 405 410 415 Glu Asp ValAsp Met Lys Gly His Asp Tyr Arg Leu Leu Pro Phe Gly 420 425 430 Ala GlyArg Arg Val Cys Pro Gly Ala Gln Leu Ala Ile Asn Leu Val 435 440 445 ThrSer Met Leu Gly His Leu Leu His His Phe Thr Trp Ala Pro Ala 450 455 460Pro Gly Val Asn Pro Glu Asp Ile Asp Leu Glu Glu Ser Pro Gly Thr 465 470475 480 Val Thr Tyr Met Lys Asn Pro Ile Gln Ala Ile Pro Thr Pro Arg Leu485 490 495 Pro Ala His Leu Tyr Gly Arg Val Pro Val Asp Met 500 505 2551580 DNA NICOTIANATABACUM 255 aatcactaat tttcatgtac tctcataggtcaaaagtttc aaccaaaatc atggctctat 60 ccttcatatt catatccata accctaatttttctagttca taaactctac caccgtctta 120 gattcaaact accaccaggt ccgcggccgttaccggtggt cggaaacctc tacgacatag 180 aaccggtgag attccggtgc tttgccgattgggccaaaac ttacggtccg attttctcag 240 tatactttgg gtcacagtta aatgttgtggtaacaacagc tgaattagct aaagaagtat 300 tgaaagaaaa tgaccagaat ttagcagatagatttaggac tagacctgca aataatttga 360 gcagaaatgg gatggatttg atttgggctgattatgggcc tcattatgtg aaagtaagga 420 agctctgtaa tcttgagctt tttactcctaaaagacttga agctcttaga cctattagag 480 aagatgaagt tactgctatg gttgaaaacattttcaagga ttgtactaag cctgataaca 540 caggtaaaag cttgttgata agagagtacttaggatcagt agcattcaac aacattacaa 600 ggttaacatt tgggaaaagg ttcatgaactcaaaaggtga gattgatgag caaggtcaag 660 aattcaaggg tattgtctct aatggcatcaaaattggcgg aaaacttccc ttggcagagt 720 atgttccatg gctccgttgg tttttcacaatggaaaacga ggcactcgtg aagcactctg 780 cacgtagaga ccggttaaca agaatgatcatggatgaaca cacactggct cgcaagaaaa 840 ctggtgatac taagcagcat tttgtcgatgcattgcttac tcttcagaag cagtatgatc 900 ttagtgatga cactgttatt ggcctcctctgggatatgat tacagcagga atggacacaa 960 caaccataac agtggaatgg gcaatggcagaactagttaa gaacccaaga gtgcaactaa 1020 aagctcaaga ggagcttgac agggtaatcggaacggatcg aatcatgtca gaaaccgatt 1080 tctctaaact tccttaccta caatgtgtagccaaagaggc tctaaggttg caccctccaa 1140 ctcctctaat gcttcctcat agggccagtgccagtgtcaa aattggtggt tatgacattc 1200 ctaaggggtc catcgtgcac gtgaacgtttgggctgtcgc tcgtgaccca gccgtgtgga 1260 agaacccgtt ggagttcaga ccagagcgcttccttgagga agacgttgac atgaagggtc 1320 acgactatcg gttattgccc tttggtgcaggaaggcgtgt ttgccccggt gcacaacttg 1380 ctatcaactt ggtcacatct atgttgggtcatttgttgca tcattttaca tgggctccgg 1440 ccccgggggt taacccggag gatattgacttggaggagag ccctggaaca gtaacttaca 1500 tgaaaaatcc aatacaagct attccaactccaagattgcc tgcacacttg tatggacgtg 1560 tgccagtgga tatgtaaaac 1580 256 520PRT NICOTIANATABACUM 256 Met Tyr Ser His Arg Ser Lys Val Ser Thr Lys IleMet Ala Leu Ser 1 5 10 15 Phe Ile Phe Ile Ser Ile Thr Leu Ile Phe LeuVal His Lys Leu Tyr 20 25 30 His Arg Leu Arg Phe Lys Leu Pro Pro Gly ProArg Pro Leu Pro Val 35 40 45 Val Gly Asn Leu Tyr Asp Ile Glu Pro Val ArgPhe Arg Cys Phe Ala 50 55 60 Asp Trp Ala Lys Thr Tyr Gly Pro Ile Phe SerVal Tyr Phe Gly Ser 65 70 75 80 Gln Leu Asn Val Val Val Thr Thr Ala GluLeu Ala Lys Glu Val Leu 85 90 95 Lys Glu Asn Asp Gln Asn Leu Ala Asp ArgPhe Arg Thr Arg Pro Ala 100 105 110 Asn Asn Leu Ser Arg Asn Gly Met AspLeu Ile Trp Ala Asp Tyr Gly 115 120 125 Pro His Tyr Val Lys Val Arg LysLeu Cys Asn Leu Glu Leu Phe Thr 130 135 140 Pro Lys Arg Leu Glu Ala LeuArg Pro Ile Arg Glu Asp Glu Val Thr 145 150 155 160 Ala Met Val Glu AsnIle Phe Lys Asp Cys Thr Lys Pro Asp Asn Thr 165 170 175 Gly Lys Ser LeuLeu Ile Arg Glu Tyr Leu Gly Ser Val Ala Phe Asn 180 185 190 Asn Ile ThrArg Leu Thr Phe Gly Lys Arg Phe Met Asn Ser Lys Gly 195 200 205 Glu IleAsp Glu Gln Gly Gln Glu Phe Lys Gly Ile Val Ser Asn Gly 210 215 220 IleLys Ile Gly Gly Lys Leu Pro Leu Ala Glu Tyr Val Pro Trp Leu 225 230 235240 Arg Trp Phe Phe Thr Met Glu Asn Glu Ala Leu Val Lys His Ser Ala 245250 255 Arg Arg Asp Arg Leu Thr Arg Met Ile Met Asp Glu His Thr Leu Ala260 265 270 Arg Lys Lys Thr Gly Asp Thr Lys Gln His Phe Val Asp Ala LeuLeu 275 280 285 Thr Leu Gln Lys Gln Tyr Asp Leu Ser Asp Asp Thr Val IleGly Leu 290 295 300 Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr ThrIle Thr Val 305 310 315 320 Glu Trp Ala Met Ala Glu Leu Val Lys Asn ProArg Val Gln Leu Lys 325 330 335 Ala Gln Glu Glu Leu Asp Arg Val Ile GlyThr Asp Arg Ile Met Ser 340 345 350 Glu Thr Asp Phe Ser Lys Leu Pro TyrLeu Gln Cys Val Ala Lys Glu 355 360 365 Ala Leu Arg Leu His Pro Pro ThrPro Leu Met Leu Pro His Arg Ala 370 375 380 Ser Ala Ser Val Lys Ile GlyGly Tyr Asp Ile Pro Lys Gly Ser Ile 385 390 395 400 Val His Val Asn ValTrp Ala Val Ala Arg Asp Pro Ala Val Trp Lys 405 410 415 Asn Pro Leu GluPhe Arg Pro Glu Arg Phe Leu Glu Glu Asp Val Asp 420 425 430 Met Lys GlyHis Asp Tyr Arg Leu Leu Pro Phe Gly Ala Gly Arg Arg 435 440 445 Val CysPro Gly Ala Gln Leu Ala Ile Asn Leu Val Thr Ser Met Leu 450 455 460 GlyHis Leu Leu His His Phe Thr Trp Ala Pro Ala Pro Gly Val Asn 465 470 475480 Pro Glu Asp Ile Asp Leu Glu Glu Ser Pro Gly Thr Val Thr Tyr Met 485490 495 Lys Asn Pro Ile Gln Ala Ile Pro Thr Pro Arg Leu Pro Ala His Leu500 505 510 Tyr Gly Arg Val Pro Val Asp Met 515 520 257 1544 DNANICOTIANATABACUM 257 ataatgctct ttctactctt tgtagccctt cctttcattcttatttttct tcttcctaaa 60 ttcaaaaatg gtggaaataa cagattgcca ccaggtcctataggtttacc attcattgga 120 aatttgcatc aatatgatag tataactcct catatctatttttggaaact ttccaaaaaa 180 tatggcaaaa tcttctcatt aaaacttgct tctactaatgtggtagtagt ttcttcagca 240 aaattagcaa aagaagtatt gaaaaaacaa gatttaatattttgtagtag accatctatt 300 cttggccaac aaaaactgtc ttattatggt cgtgatattgcttttgcacc ttataatgat 360 tattggagag aaatgagaaa aatttgtgtt cttcatctttttagtttaaa aaaagttcaa 420 ttatttagtc caattcgtga agatgaagtt tttagaatgattaagaaaat atcaaaacaa 480 gcttctactt cacaaattat taatttgagt aatttaatgatttcattaac aagtacaatt 540 atttgtagag ttgcttttgg tgttaggttt gaagaagaagcacatgcaag gaagagattt 600 gattttcttt tggccgaggc acaagaaatg atggctagtttctttgtatc tgattttttt 660 ccctttttaa gttagattga caaattaagt ggattgacatatagacttga gaggaatttc 720 aaggatttgg ataattttta tgaagaactc attgagcaacatcaaaatcc taataagcca 780 aaatatatgg aaggagatat tgttgatctt ttgctacaattgaagaaaga gaaattaaca 840 ccacttgatc tcactatgga agatataaaa ggaattctcatgaatgtgtt agttgcagga 900 tcagacacta gtgcagctgc tactgtttgg gcaatgacagccttgataaa gaatcctaaa 960 gccatggaaa aagttcaatt agaaatcaga aaatcagttgggaagaaagg cattgtaaat 1020 gaagaagatg tccaaaacat cccttatttt aaagcagtgataaaggaaat atttagattg 1080 tatccaccag ctccactttt agttccaaga gaatcaatggaaaaaaccat attagaaggt 1140 tatgaaattc ggccaagaac catagttcat gttaacgcttgggctatagc aagggatcct 1200 gaaatatggg aaaatccaga tgaatttata cctgagagatttttgaatag cagtatcgat 1260 tacaagggtc aagattttga gttacttcca tttggtgcaggcagaagagg ttgcccaggt 1320 attgcacttg gggttgcatc catggaactt gctttgtcaaatcttcttta tgcatttgat 1380 tgggagttgc cttatggagt gaaaaaagaa gacatcgacacaaacgttag gcctggaatt 1440 gccatgcaca agaaaaacga actttgcctt gtcccaaaaaaattatttat aaattatatt 1500 gggacgtgga tctcatgcta gttctgtgcg gtcagctaagctta 1544 258 504 PRT NICOTIANATABACUM 258 Met Leu Phe Leu Leu Phe ValAla Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys AsnGly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe IleGly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe TrpLys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala SerThr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu ValLeu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu GlyGln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala ProTyr Asn Asp Tyr Trp Arg Glu Met Arg Lys Ile Cys 115 120 125 Val Leu HisLeu Phe Ser Leu Lys Lys Val Gln Leu Phe Ser Pro Ile 130 135 140 Arg GluAsp Glu Val Phe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala 145 150 155 160Ser Thr Ser Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr 165 170175 Ser Thr Ile Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu 180185 190 Ala His Ala Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu195 200 205 Met Met Ala Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu SerIle 210 215 220 Asp Lys Leu Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn PheLys Asp 225 230 235 240 Leu Asp Asn Phe Tyr Glu Glu Leu Ile Glu Gln HisGln Asn Pro Asn 245 250 255 Lys Pro Lys Tyr Met Glu Gly Asp Ile Val AspLeu Leu Leu Gln Leu 260 265 270 Lys Lys Glu Lys Leu Thr Pro Leu Asp LeuThr Met Glu Asp Ile Lys 275 280 285 Gly Ile Leu Met Asn Val Leu Val AlaGly Ser Asp Thr Ser Ala Ala 290 295 300 Ala Thr Val Trp Ala Met Thr AlaLeu Ile Lys Asn Pro Lys Ala Met 305 310 315 320 Glu Lys Val Gln Leu GluIle Arg Lys Ser Val Gly Lys Lys Gly Ile 325 330 335 Val Asn Glu Glu AspVal Gln Asn Ile Pro Tyr Phe Lys Ala Val Ile 340 345 350 Lys Glu Ile PheArg Leu Tyr Pro Pro Ala Pro Leu Leu Val Pro Arg 355 360 365 Glu Ser MetGlu Lys Thr Ile Leu Glu Gly Tyr Glu Ile Arg Pro Arg 370 375 380 Thr IleVal His Val Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu Ile 385 390 395 400Trp Glu Asn Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser Ser 405 410415 Ile Asp Tyr Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala Gly 420425 430 Arg Arg Gly Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu Leu435 440 445 Ala Leu Ser Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro TyrGly 450 455 460 Val Lys Lys Glu Asp Ile Asp Thr Asn Val Arg Pro Gly IleAla Met 465 470 475 480 His Lys Lys Asn Glu Leu Cys Leu Val Pro Lys LysLeu Phe Ile Asn 485 490 495 Tyr Ile Gly Thr Trp Ile Ser Cys 500 259 1615DNA NICOTIANATABACUM 259 cacattgagt cctctcccaa atcactgatt caccaccaaaagtaccaaca attcaatgga 60 aggtacaaac ttgactacat atgcagcagt atttcttgatactctgtttc ttttgttcct 120 ttccaaactt cttcgccaga ggaaactcaa tttacctccaggcccaaaac catggccgat 180 catcggaaac ttaaacctta ttggcaatct tcctcatcgctcaatccacg aactctccct 240 caagtacgga cccgttatgc aactccaatt cgggtctttccccgttgtag ttggatcctc 300 cgtcgaaatg gctaagattt tcctcaaatc catggatattaactttgtag gcaggcctaa 360 aacggctgcc ggaaaataca caacgtacaa ttattccgatattacatggt ctccttacgg 420 accatattgg cgccaggcac gtaggatgtg cctaacggaattattcagca cgaaacgtct 480 cgattcatac gagtatattc gggctgagga gttgcattctcttctccata atttgaacaa 540 aatatcaggg aaaccaattg tgttgaaaga ttatttgacgacgttgagtt taaatgttat 600 tagcaggatg gtactgggga aaaggtattt ggacgaatccgagaactcgt tcgtgaatcc 660 tgaggaattt aagaagatgt tggacgaatt gtttttgctaaatggtgtac ttaatattgg 720 agattcaatt ccatggattg atttcatgga tttgcaaggttatgttaaga ggatgaaagt 780 agtgagcaag aaattcgaca agtttttaga gcatgttattgatgagcata acattaggag 840 aaatggagtg gagaattatg ttgctaagga tatggtggatgttttgttgc agcttgctga 900 tgatccgaag ttggaagtta agctggagag acatggagtcaaagcattca ctcaggatat 960 gctggctggt ggaaccgaga gttcagcagt gacagtggagtgggcaattt cagagctgct 1020 aaagaagccg gagattttca aaaaggctac agaagaattggatcgagtaa ttgggcagaa 1080 tagatgggta caagaaaagg acattccaaa tcttccttacatagaggcaa tagtcaaaga 1140 gactatgcga ctgcaccccg tggcaccaat gttggtgccacgtgagtgtc gagaagatat 1200 taaggtagca ggctacgacg ttcagaaagg aactagggttctcgtgagtg tatggactat 1260 tggaagagac cctacattgt gggacgagcc tgaggtgttcaagccggaga gattccatga 1320 aaagtccata gatgttaaag gacatgatta tgagcttttgccatttggag cggggagaag 1380 aatgtgcccg ggttatagct tggggctcaa ggtgattcaagctagcttag ctaatcttct 1440 acatggattt aactggtcat tgcctgataa tatgactcctgaggacctca acatggatga 1500 gatttttggg ctctctacac ctaaaaaatt tccacttgctactgtgattg agccaagact 1560 ttcaccaaaa ctttactctg tttgattcag cagttctatggttccgtcaa gatag 1615 260 509 PRT NICOTIANATABACUM 260 Met Glu Gly ThrAsn Leu Thr Thr Tyr Ala Ala Val Phe Leu Asp Thr 1 5 10 15 Leu Phe LeuLeu Phe Leu Ser Lys Leu Leu Arg Gln Arg Lys Leu Asn 20 25 30 Leu Pro ProGly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Ile Gly AsnLeu Pro His Arg Ser Ile His Glu Leu Ser Leu Lys Tyr 50 55 60 Gly Pro ValMet Gln Leu Gln Phe Gly Ser Phe Pro Val Val Val Gly 65 70 75 80 Ser SerVal Glu Met Ala Lys Ile Phe Leu Lys Ser Met Asp Ile Asn 85 90 95 Phe ValGly Arg Pro Lys Thr Ala Ala Gly Lys Tyr Thr Thr Tyr Asn 100 105 110 TyrSer Asp Ile Thr Trp Ser Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125Arg Arg Met Cys Leu Thr Glu Leu Phe Ser Thr Lys Arg Leu Asp Ser 130 135140 Tyr Glu Tyr Ile Arg Ala Glu Glu Leu His Ser Leu Leu His Asn Leu 145150 155 160 Asn Lys Ile Ser Gly Lys Pro Ile Val Leu Lys Asp Tyr Leu ThrThr 165 170 175 Leu Ser Leu Asn Val Ile Ser Arg Met Val Leu Gly Lys ArgTyr Leu 180 185 190 Asp Glu Ser Glu Asn Ser Phe Val Asn Pro Glu Glu PheLys Lys Met 195 200 205 Leu Asp Glu Leu Phe Leu Leu Asn Gly Val Leu AsnIle Gly Asp Ser 210 215 220 Ile Pro Trp Ile Asp Phe Met Asp Leu Gln GlyTyr Val Lys Arg Met 225 230 235 240 Lys Val Val Ser Lys Lys Phe Asp LysPhe Leu Glu His Val Ile Asp 245 250 255 Glu His Asn Ile Arg Arg Asn GlyVal Glu Asn Tyr Val Ala Lys Asp 260 265 270 Met Val Asp Val Leu Leu GlnLeu Ala Asp Asp Pro Lys Leu Glu Val 275 280 285 Lys Leu Glu Arg His GlyVal Lys Ala Phe Thr Gln Asp Met Leu Ala 290 295 300 Gly Gly Thr Glu SerSer Ala Val Thr Val Glu Trp Ala Ile Ser Glu 305 310 315 320 Leu Leu LysLys Pro Glu Ile Phe Lys Lys Ala Thr Glu Glu Leu Asp 325 330 335 Arg ValIle Gly Gln Asn Arg Trp Val Gln Glu Lys Asp Ile Pro Asn 340 345 350 LeuPro Tyr Ile Glu Ala Ile Val Lys Glu Thr Met Arg Leu His Pro 355 360 365Val Ala Pro Met Leu Val Pro Arg Glu Cys Arg Glu Asp Ile Lys Val 370 375380 Ala Gly Tyr Asp Val Gln Lys Gly Thr Arg Val Leu Val Ser Val Trp 385390 395 400 Thr Ile Gly Arg Asp Pro Thr Leu Trp Asp Glu Pro Glu Val PheLys 405 410 415 Pro Glu Arg Phe His Glu Lys Ser Ile Asp Val Lys Gly HisAsp Tyr 420 425 430 Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Met Cys ProGly Tyr Ser 435 440 445 Leu Gly Leu Lys Val Ile Gln Ala Ser Leu Ala AsnLeu Leu His Gly 450 455 460 Phe Asn Trp Ser Leu Pro Asp Asn Met Thr ProGlu Asp Leu Asn Met 465 470 475 480 Asp Glu Ile Phe Gly Leu Ser Thr ProLys Lys Phe Pro Leu Ala Thr 485 490 495 Val Ile Glu Pro Arg Leu Ser ProLys Leu Tyr Ser Val 500 505 261 1610 DNA NICOTIANATABACUM 261 cttcttccttcctaactaaa aatggagatt cagttttcta acttagttgc attcttgctc 60 tttctctccagcatctttct tgtattcaaa aaatggaaaa ccagaaaact aaatttgcct 120 cctggtccatggaaattacc ttttattgga agtttacacc atttggctgt ggcaggtcca 180 cttcctcaccatggcctaaa aaatttagcc aaacgctatg gtcctcttat gcatttacaa 240 cttggacaaattcctacact cgtcatatca tcacctcaaa tggcaaaaga agtactaaaa 300 actcacgacctcgcttttgc cactagacca aagcttgtcg tggccgacat cattcactac 360 gacagcacggacatagcact ttcgccatac ggtgaatact ggagacaaat tcgtaaaatt 420 tgcatattggaactcttgag tgccaagatg gtcaagtttt ttagctcgat tcgccaagat 480 gagctctcgaagatggtttc atctatacga acgacgccca atcttccagt caatcttacc 540 gacaagattttttggtttac gagttcggta atttgtagat cagctttagg gaagatatgt 600 ggtgaccaagacaaattgat catttttatg agggaaataa tatcattggc aggtggattt 660 agtattgctgattttttccc tacatggaaa atgattcatg atattgatgg ttcaaaatct 720 aaactggtgaaggcacatcg taagattgat gaaattttgg aaaatgtggt aaatgagcac 780 aaacagaatcgagcagatgg taaaaagggt aatggtgaat ttggtggaga agatctgatt 840 gatgttttgttaagagttag agaaagtgga gaagttcaaa ttccaatcac agatgacaat 900 atcaaatcaatattaatcga catgttctct gccggatcgg aaacatcatc gacaactata 960 atttgggcattagctgaaat gatgaagaaa ccaagtgttt tagcaaaggc acaagctgaa 1020 gtgagccaagctttgaaggg gaagaaaatt agttttcaag agattgatat tgataagcta 1080 aagtatttgaagttagtgat caaagaaact ttaagaatgc accctccaat tcctctgtta 1140 gtccctagagaatgtatgga agatacaaag attgatggtt acaatatacc tttcaaaaca 1200 agagtcattgttaatgcatg ggcaattgga cgagatcctc aaagttggga tgatcctgaa 1260 agctttacgccagagagatt tgagaataat tctattgatt ttcttggaaa tcatcatcaa 1320 tttattccatttggtgcagg aagaaggatt tgtcctggaa tgctatttgg tttagctaat 1380 gttggacaacctttagctca gttactttat cacttcgatt ggaaactccc taatggacaa 1440 actcaccaaaatttcgacat gactgagtca cctggaattt ctgctacaag aaaggatgat 1500 cttattttgattgccactcc tgctcattct tgattaagta ttgctgcttt tctattggag 1560 aattttcaaaattcatccac aatatatagt gtttgctaga gttggttagc 1610 262 503 PRTNICOTIANATABACUM 262 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu LeuPhe Leu Ser 1 5 10 15 Ser Ile Phe Leu Val Phe Lys Lys Trp Lys Thr ArgLys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly SerLeu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu LysAsn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly GlnIle Pro Thr Leu 65 70 75 80 Val Ile Ser Ser Pro Gln Met Ala Lys Glu ValLeu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val ValAla Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Leu Ser ProTyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu GluLeu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg GlnAsp Glu Leu Ser Lys Met Val Ser 145 150 155 160 Ser Ile Arg Thr Thr ProAsn Leu Pro Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr SerSer Val Ile Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp GlnAsp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala GlyGly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile HisAsp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240Lys Ile Asp Glu Ile Leu Glu Asn Val Val Asn Glu His Lys Gln Asn 245 250255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro275 280 285 Ile Thr Asp Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe SerAla 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu AlaGlu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln AlaGlu Val Ser Gln 325 330 335 Ala Leu Lys Gly Lys Lys Ile Ser Phe Gln GluIle Asp Ile Asp Lys 340 345 350 Leu Lys Tyr Leu Lys Leu Val Ile Lys GluThr Leu Arg Met His Pro 355 360 365 Pro Ile Pro Leu Leu Val Pro Arg GluCys Met Glu Asp Thr Lys Ile 370 375 380 Asp Gly Tyr Asn Ile Pro Phe LysThr Arg Val Ile Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp ProGln Ser Trp Asp Asp Pro Glu Ser Phe Thr 405 410 415 Pro Glu Arg Phe GluAsn Asn Ser Ile Asp Phe Leu Gly Asn His His 420 425 430 Gln Phe Ile ProPhe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu 435 440 445 Phe Gly LeuAla Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His 450 455 460 Phe AspTrp Lys Leu Pro Asn Gly Gln Thr His Gln Asn Phe Asp Met 465 470 475 480Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Ile Leu 485 490495 Ile Ala Thr Pro Ala His Ser 500 263 1611 DNA NICOTIANATABACUM 263ctttcatcat atggcatgaa atgggaaatg ctcacaacag caaaattgca gcaatctgtt 60tgataatttt cttggtatat aaagcatggg aattgttgaa gtggatatgg attaagccaa 120agaaactgga gagttgcctc agaaaacagg gactcaaagg aaattcctac gggctattct 180atggagatat gaaagaattg tccaaaagtc tcaaggaaat caattcaaag cccatcatca 240atctatcaaa tgaagtagcc ccaagaatca ttccttatta tcttgaaatc atccaaaaat 300atggtaaaag atgttttgtt tggcaaggac caacccccgc aatattaata acagagccag 360aattaataaa ggagatattt ggtaagaact atgtttttca gaagcctaat aatcccaacc 420cactgaccaa gttattggct cgaggtgttg taagctacga ggaagaaaaa tgggcaaaac 480acagaaagat cttaaatcct gcctttcata tggagaagtt gaagcatatg ctaccagcat 540tttacttgag ctgtagtgag atgctgaaca aatgggagga gattatccca gtaaaagaat 600caaatgagtt ggacatttgg cctcatcttc aaagaatgac aagtgatgtg atttctcgtg 660ctgcctttgg tagtagctac gaagaaggaa gaagaatatt tgaacttcaa gaagaacaag 720ctgagtatct aacgaagaca ttcaattcag tttatatccc aggttccaga ttttttccca 780ataaaatgaa caaaagaatg aaagaatgtg aaaaggaagt acgagaaaca attacgtgtc 840taattgacaa cagattaaag gcaaaagaag aaggcaatgg caaggccctc aatgatgacc 900tattgggtat attattagag tcaaattcta tagaaattga agaacatggt aacaagaagt 960ttggaatgag tatacctgaa gtaattgaag agtgcaaatt attctatttt gctggccaag 1020agactacatc agtattgctt gtgtggacac tgattttgtt agggagaaat ccagaatggc 1080aggaacgtgc tagagaggaa gtttttcaag cctttggaag tgataaacca acttttgacg 1140aattatatcg cttgaaaatt gtgacgatga ttttgtacga gtctttaagg ttatatccac 1200caatagcaac tcgtactcga aggactaatg aagaaacaaa attaggggaa ctagatttac 1260caaagggtgc actgctcttt ataccaacaa tcttattaca tcttgacagg gaaatttggg 1320gtgaagatgc agatgagttc aatccggaga gatttagcga aggggtggca aaggcaacaa 1380aggggaaaat gacatatttt ccatttggtg caggaccgcg aaaatgcatt gggcaaaact 1440tcgcgatttt ggaagcaaaa atggctatag ctatgattct acaacgcttc tccttcgagc 1500tctctccatc ttatacacac tctccataca ctgtggtcac tttgaaaccc aaatatggtg 1560ctcccctaat aatgcacagg ctgtagtcct gtgagaatat gctatccgag g 1611 264 521PRT NICOTIANATABACUM 264 Met Gly Asn Ala His Asn Ser Lys Ile Ala Ala IleCys Leu Ile Ile 1 5 10 15 Phe Leu Val Tyr Lys Ala Trp Glu Leu Leu LysTrp Ile Trp Ile Lys 20 25 30 Pro Lys Lys Leu Glu Ser Cys Leu Arg Lys GlnGly Leu Lys Gly Asn 35 40 45 Ser Tyr Gly Leu Phe Tyr Gly Asp Met Lys GluLeu Ser Lys Ser Leu 50 55 60 Lys Glu Ile Asn Ser Lys Pro Ile Ile Asn LeuSer Asn Glu Val Ala 65 70 75 80 Pro Arg Ile Ile Pro Tyr Tyr Leu Glu IleIle Gln Lys Tyr Gly Lys 85 90 95 Arg Cys Phe Val Trp Gln Gly Pro Thr ProAla Ile Leu Ile Thr Glu 100 105 110 Pro Glu Leu Ile Lys Glu Ile Phe GlyLys Asn Tyr Val Phe Gln Lys 115 120 125 Pro Asn Asn Pro Asn Pro Leu ThrLys Leu Leu Ala Arg Gly Val Val 130 135 140 Ser Tyr Glu Glu Glu Lys TrpAla Lys His Arg Lys Ile Leu Asn Pro 145 150 155 160 Ala Phe His Met GluLys Leu Lys His Met Leu Pro Ala Phe Tyr Leu 165 170 175 Ser Cys Ser GluMet Leu Asn Lys Trp Glu Glu Ile Ile Pro Val Lys 180 185 190 Glu Ser AsnGlu Leu Asp Ile Trp Pro His Leu Gln Arg Met Thr Ser 195 200 205 Asp ValIle Ser Arg Ala Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 ArgIle Phe Glu Leu Gln Glu Glu Gln Ala Glu Tyr Leu Thr Lys Thr 225 230 235240 Phe Asn Ser Val Tyr Ile Pro Gly Ser Arg Phe Phe Pro Asn Lys Met 245250 255 Asn Lys Arg Met Lys Glu Cys Glu Lys Glu Val Arg Glu Thr Ile Thr260 265 270 Cys Leu Ile Asp Asn Arg Leu Lys Ala Lys Glu Glu Gly Asn GlyLys 275 280 285 Ala Leu Asn Asp Asp Leu Leu Gly Ile Leu Leu Glu Ser AsnSer Ile 290 295 300 Glu Ile Glu Glu His Gly Asn Lys Lys Phe Gly Met SerIle Pro Glu 305 310 315 320 Val Ile Glu Glu Cys Lys Leu Phe Tyr Phe AlaGly Gln Glu Thr Thr 325 330 335 Ser Val Leu Leu Val Trp Thr Leu Ile LeuLeu Gly Arg Asn Pro Glu 340 345 350 Trp Gln Glu Arg Ala Arg Glu Glu ValPhe Gln Ala Phe Gly Ser Asp 355 360 365 Lys Pro Thr Phe Asp Glu Leu TyrArg Leu Lys Ile Val Thr Met Ile 370 375 380 Leu Tyr Glu Ser Leu Arg LeuTyr Pro Pro Ile Ala Thr Arg Thr Arg 385 390 395 400 Arg Thr Asn Glu GluThr Lys Leu Gly Glu Leu Asp Leu Pro Lys Gly 405 410 415 Ala Leu Leu PheIle Pro Thr Ile Leu Leu His Leu Asp Arg Glu Ile 420 425 430 Trp Gly GluAsp Ala Asp Glu Phe Asn Pro Glu Arg Phe Ser Glu Gly 435 440 445 Val AlaLys Ala Thr Lys Gly Lys Met Thr Tyr Phe Pro Phe Gly Ala 450 455 460 GlyPro Arg Lys Cys Ile Gly Gln Asn Phe Ala Ile Leu Glu Ala Lys 465 470 475480 Met Ala Ile Ala Met Ile Leu Gln Arg Phe Ser Phe Glu Leu Ser Pro 485490 495 Ser Tyr Thr His Ser Pro Tyr Thr Val Val Thr Leu Lys Pro Lys Tyr500 505 510 Gly Ala Pro Leu Ile Met His Arg Leu 515 520 265 1570 DNANICOTIANATABACUM 265 ctcattatcc atcacctaaa atggagaatt cttgggtttttctagccttg gcagggctat 60 ctgcattagc ttttctctgt aaaataatca cctgtcgaagaccggttaac cggaaaatac 120 caccaggtcc aaaaccatgg cccatcattg gcaatttgaacctacttggt cctataccac 180 atcaatcttt tgacttgctt tccaaaaaat atggagagttgatgctgctg aaatttggct 240 ccaggccagt tcttgttgct tcatctgctg aaatggcaaaacagttttta aaagtacatg 300 atgctaattt cgcctcccgt cctatgctag ctggtggaaagtatacaagc tataactatt 360 gtgacatgac atgggcaccc tatggtccct attggcgccaagcacgacga atttacctta 420 accagatatt tactccgaaa aggctagact cgttcgagtacattcgtgtt gaagaaaggc 480 aggccttgat ttcccagctg aattcccttg ctggaaagccattttttctc aaagaccatt 540 tgtcgcgatt tagcctctgc agcatgacaa ggatggttttgagcaacaag tattttggtg 600 aatcaacagt tagagtagaa gatttgcagt acctggtagatcaatggttc ttacttaatg 660 gtgctttcaa cattggagat tggattccat ggctcagcttcttggaccta caaggctatg 720 tgaaacaaat gaaggctttg aaaagaactt ttgataagttccacaacatt gtgctagatg 780 atcacagggc taagaagaat gcagagaaga actttgtcccaaaagacatg gttgatgtct 840 tgttgaagat ggctgaagat cctaatctgg aagtcaaactcactaatgac tgtgtcaaag 900 ggttaatgca ggatttacta actggaggaa cagatagcttaacagcagca gtgcaatggg 960 catttcaaga acttcttaga cagccaaggg ttattgagaaggcaaccgaa gagcttgacc 1020 ggattgtcgg gaaagagaga tgggtagaag agaaagattgctcgcagcta tcttacgttg 1080 aagcaatcct caaggaaaca ctaaggttac atcctctaggaactatgcta gcaccgcatt 1140 gtgctataga agattgtaac gtggctggtt atgacatacagaaaggaacg acctttctgg 1200 tgaatgtttg gaccattgga agggacccaa aatactgggatagagcacaa gagtttctcc 1260 ccgagagatt tttagagaac gacattgata tggacggacataactttgct ttcttgccat 1320 ttggctcggg gcgaaggagg tgccctggct atagccttggacttaaggtt atccgagtaa 1380 cattagccaa catgttgcat ggattcaact ggaaattacctgaaggtatg aagccagaag 1440 atataagtgt ggaagaacat tatgggctca ctacacatcctaagtttcct gttcctgtga 1500 tcttggaatc tagactttct tcagatctct attcccccatcacttaatcc taagtgcttc 1560 ctattatagc 1570 266 508 PRT NICOTIANATABACUM266 Met Glu Asn Ser Trp Val Phe Leu Ala Leu Ala Gly Leu Ser Ala Leu 1 510 15 Ala Phe Leu Cys Lys Ile Ile Thr Cys Arg Arg Pro Val Asn Arg Lys 2025 30 Ile Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 3540 45 Leu Gly Pro Ile Pro His Gln Ser Phe Asp Leu Leu Ser Lys Lys Tyr 5055 60 Gly Glu Leu Met Leu Leu Lys Phe Gly Ser Arg Pro Val Leu Val Ala 6570 75 80 Ser Ser Ala Glu Met Ala Lys Gln Phe Leu Lys Val His Asp Ala Asn85 90 95 Phe Ala Ser Arg Pro Met Leu Ala Gly Gly Lys Tyr Thr Ser Tyr Asn100 105 110 Tyr Cys Asp Met Thr Trp Ala Pro Tyr Gly Pro Tyr Trp Arg GlnAla 115 120 125 Arg Arg Ile Tyr Leu Asn Gln Ile Phe Thr Pro Lys Arg LeuAsp Ser 130 135 140 Phe Glu Tyr Ile Arg Val Glu Glu Arg Gln Ala Leu IleSer Gln Leu 145 150 155 160 Asn Ser Leu Ala Gly Lys Pro Phe Phe Leu LysAsp His Leu Ser Arg 165 170 175 Phe Ser Leu Cys Ser Met Thr Arg Met ValLeu Ser Asn Lys Tyr Phe 180 185 190 Gly Glu Ser Thr Val Arg Val Glu AspLeu Gln Tyr Leu Val Asp Gln 195 200 205 Trp Phe Leu Leu Asn Gly Ala PheAsn Ile Gly Asp Trp Ile Pro Trp 210 215 220 Leu Ser Phe Leu Asp Leu GlnGly Tyr Val Lys Gln Met Lys Ala Leu 225 230 235 240 Lys Arg Thr Phe AspLys Phe His Asn Ile Val Leu Asp Asp His Arg 245 250 255 Ala Lys Lys AsnAla Glu Lys Asn Phe Val Pro Lys Asp Met Val Asp 260 265 270 Val Leu LeuLys Met Ala Glu Asp Pro Asn Leu Glu Val Lys Leu Thr 275 280 285 Asn AspCys Val Lys Gly Leu Met Gln Asp Leu Leu Thr Gly Gly Thr 290 295 300 AspSer Leu Thr Ala Ala Val Gln Trp Ala Phe Gln Glu Leu Leu Arg 305 310 315320 Gln Pro Arg Val Ile Glu Lys Ala Thr Glu Glu Leu Asp Arg Ile Val 325330 335 Gly Lys Glu Arg Trp Val Glu Glu Lys Asp Cys Ser Gln Leu Ser Tyr340 345 350 Val Glu Ala Ile Leu Lys Glu Thr Leu Arg Leu His Pro Leu GlyThr 355 360 365 Met Leu Ala Pro His Cys Ala Ile Glu Asp Cys Asn Val AlaGly Tyr 370 375 380 Asp Ile Gln Lys Gly Thr Thr Phe Leu Val Asn Val TrpThr Ile Gly 385 390 395 400 Arg Asp Pro Lys Tyr Trp Asp Arg Ala Gln GluPhe Leu Pro Glu Arg 405 410 415 Phe Leu Glu Asn Asp Ile Asp Met Asp GlyHis Asn Phe Ala Phe Leu 420 425 430 Pro Phe Gly Ser Gly Arg Arg Arg CysPro Gly Tyr Ser Leu Gly Leu 435 440 445 Lys Val Ile Arg Val Thr Leu AlaAsn Met Leu His Gly Phe Asn Trp 450 455 460 Lys Leu Pro Glu Gly Met LysPro Glu Asp Ile Ser Val Glu Glu His 465 470 475 480 Tyr Gly Leu Thr ThrHis Pro Lys Phe Pro Val Pro Val Ile Leu Glu 485 490 495 Ser Arg Leu SerSer Asp Leu Tyr Ser Pro Ile Thr 500 505 267 1617 DNA NICOTIANATABACUM267 cacattgagt cctctcccaa atcactgatt caccaccaaa agtaccaaca attcaatgga 60aggtacaaac ttgactacat atgcagcagt atttcttgat actctgtttc ttttgttcct 120ttccaaactt cttcgccaga ggaaactcaa tttacctcca ggcccaaaac catggccgat 180catcggaaac ttaaacctta ttggcaatct tcctcatcgc tcaatccacg aactctccct 240caagtacgga cccgttatgc aactccaatt cgggtctttc cccgttgtag ttggatcctc 300cgtcgaaatg gctaagattt tcctcaaatc catggatatt aactttgtag gcaggcctaa 360aacggctgcc ggaaaataca caacgtacaa ttattccgat attacatggt ctccttacgg 420accatattgg cgccaggcac gtaggatgtg cctaacggaa ttattcagca cgaaacgtct 480cgattcatac gagtatattc gggctgagga gttgcattct cttctccata atttgaacaa 540aatatcaggg aaaccaattg tgttgaaaga ttatttgacg acgttgagtt taaatgttat 600tagcaggatg gtactgggga aaaggtattt ggacgaatcc gagaactcgt tcgtgaatcc 660tgaggaattt aagaagatgt tggacgaatt gtttttgcta aatggtgtac ttaatattgg 720agattcaatt ccatggattg atttcatgga tttgcaaggt tatgttaaga ggatgaaagt 780agtgagcaag aaattcgaca agtttttaga gcatgttatt gatgagcata acattaggag 840aaatggagtg gagaattatg ttgctaagga tatggtggat gttttgttgc agcttgctga 900tgatccgaag ttggaagtta agctggagag acatggagtc aaagcattca ctcaggatat 960gctggctggt ggaaccgaga gttcagcagt gacagtggag tgggcaattt cagagctgct 1020aaagaagccg gagattttca aaaaggctac agaagaattg gatcgagtaa ttgggcagaa 1080tagatgggta caagaaaagg acattccaaa tcatccttac atagaggcaa tagtcaaaga 1140gactatgcga ctgcaccccg tggcaccaat gttggtgcca cgtgagtgtc gagaagatat 1200taaggtagca ggctacgacg ttcagaaagg aactagggtt ctcgtgagtg tatggactat 1260tggaagagac cctacattgt gggacgagcc tgaggtgttc aagccggaga gattccatga 1320aaagtccata gatgttaaag gacatgatta tgagcttttg ccatttggag cggggagaag 1380aatgtgcccg ggttatagct tggggctcaa ggtgattcaa gctagcttag ctaatcttct 1440acatggattt aactggtcat tgcctgataa tatgactcct gaggacctca acatggatga 1500gatttttggg ctctctacac ctaaaaaatt tccacttgct actgtgattg agccaagact 1560ttcaccaaaa ctttactctg tttgattcag cagttctatg gatccgtcaa gatagac 1617 268509 PRT NICOTIANATABACUM 268 Met Glu Gly Thr Asn Leu Thr Thr Tyr Ala AlaVal Phe Leu Asp Thr 1 5 10 15 Leu Phe Leu Leu Phe Leu Ser Lys Leu LeuArg Gln Arg Lys Leu Asn 20 25 30 Leu Pro Pro Gly Pro Lys Pro Trp Pro IleIle Gly Asn Leu Asn Leu 35 40 45 Ile Gly Asn Leu Pro His Arg Ser Ile HisGlu Leu Ser Leu Lys Tyr 50 55 60 Gly Pro Val Met Gln Leu Gln Phe Gly SerPhe Pro Val Val Val Gly 65 70 75 80 Ser Ser Val Glu Met Ala Lys Ile PheLeu Lys Ser Met Asp Ile Asn 85 90 95 Phe Val Gly Arg Pro Lys Thr Ala AlaGly Lys Tyr Thr Thr Tyr Asn 100 105 110 Tyr Ser Asp Ile Thr Trp Ser ProTyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Met Cys Leu Thr GluLeu Phe Ser Thr Lys Arg Leu Asp Ser 130 135 140 Tyr Glu Tyr Ile Arg AlaGlu Glu Leu His Ser Leu Leu His Asn Leu 145 150 155 160 Asn Lys Ile SerGly Lys Pro Ile Val Leu Lys Asp Tyr Leu Thr Thr 165 170 175 Leu Ser LeuAsn Val Ile Ser Arg Met Val Leu Gly Lys Arg Tyr Leu 180 185 190 Asp GluSer Glu Asn Ser Phe Val Asn Pro Glu Glu Phe Lys Lys Met 195 200 205 LeuAsp Glu Leu Phe Leu Leu Asn Gly Val Leu Asn Ile Gly Asp Ser 210 215 220Ile Pro Trp Ile Asp Phe Met Asp Leu Gln Gly Tyr Val Lys Arg Met 225 230235 240 Lys Val Val Ser Lys Lys Phe Asp Lys Phe Leu Glu His Val Ile Asp245 250 255 Glu His Asn Ile Arg Arg Asn Gly Val Glu Asn Tyr Val Ala LysAsp 260 265 270 Met Val Asp Val Leu Leu Gln Leu Ala Asp Asp Pro Lys LeuGlu Val 275 280 285 Lys Leu Glu Arg His Gly Val Lys Ala Phe Thr Gln AspMet Leu Ala 290 295 300 Gly Gly Thr Glu Ser Ser Ala Val Thr Val Glu TrpAla Ile Ser Glu 305 310 315 320 Leu Leu Lys Lys Pro Glu Ile Phe Lys LysAla Thr Glu Glu Leu Asp 325 330 335 Arg Val Ile Gly Gln Asn Arg Trp ValGln Glu Lys Asp Ile Pro Asn 340 345 350 His Pro Tyr Ile Glu Ala Ile ValLys Glu Thr Met Arg Leu His Pro 355 360 365 Val Ala Pro Met Leu Val ProArg Glu Cys Arg Glu Asp Ile Lys Val 370 375 380 Ala Gly Tyr Asp Val GlnLys Gly Thr Arg Val Leu Val Ser Val Trp 385 390 395 400 Thr Ile Gly ArgAsp Pro Thr Leu Trp Asp Glu Pro Glu Val Phe Lys 405 410 415 Pro Glu ArgPhe His Glu Lys Ser Ile Asp Val Lys Gly His Asp Tyr 420 425 430 Glu LeuLeu Pro Phe Gly Ala Gly Arg Arg Met Cys Pro Gly Tyr Ser 435 440 445 LeuGly Leu Lys Val Ile Gln Ala Ser Leu Ala Asn Leu Leu His Gly 450 455 460Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu Asn Met 465 470475 480 Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu Ala Thr485 490 495 Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 500 505269 1657 DNA NICOTIANATABACUM 269 tgcaatatag ttttcctagt cagttctagcctccttttcc ttagaaataa tggattatca 60 tatttctttc cattttcaag ctcttttagggcttttagcc tttgtgttct tgtctattat 120 cttatggaga agaacactca cttcaagaaaattagcccct gaaatcccag gggcatggcc 180 tattataggc catcttcgtc agctgagtggtactgataag aatatcccat ttccccgaat 240 attgggcgct ttggcagata aatatggacctgtcttcaca ctgagaatag ggatgtaccc 300 ctatttgatt gtcaacaatt gggaagcagctaaggattgt ctcacaacgc atgataagga 360 cttcgctgcc cgaccaactt ctatggctggtgaaagcatc gggtacaagt atgcgaggtt 420 tacttatgct aattttggtc cttattataaccaagtgcgc aaactagccc tacaacatgt 480 actctcgagt actaaactcg agaaaatgaaacacatacgt gtttctgaat tggaaactag 540 catcaaagaa ttatattctt tgacgctgggcaaaaacaac atgcaaaaag tgaatataag 600 taaatggttt gaacaattga ctttaaacataatcgtgaag acaatttgtg gcaagagata 660 tagcaacata gaggaggatg aagaggcacaacgtttcaga aaggcattta agggcatcat 720 gtttgttgta gggcaaattg ttttatatgacgcaattcca ttcccattgt tcaaatactt 780 tgatttccaa ggtcatatac aattgatgaacaaaatttat aaagacttag attctattct 840 tcaaggatgg ttggatgatc atatgatgaacaaggatgta aacaataagg atcaagatgc 900 catagatgcc atgcttaagg taacacaacttaatgaattc aaagcctatg gtttttctca 960 ggccactgtg atcaagtcga cagtcttgagtttgatctta gatggaaatg acacaaccgc 1020 tgttcatttg atatgggtaa tgtccttattactgaacaat ccacatgtta tgaaacaagg 1080 ccaagaagag atagacatga aagtgggtaaagagaggtgg attgaagata ctgacataaa 1140 aaatttagtg taccttcagg ctatcgttaaagagacattg cgcttgtatc cacctgttcc 1200 ttttctttta ccacacgaag cagtgcaagattgtaaagtg actggttacc acattcctaa 1260 aggtactcgt ctatatatca atgcgtggaaagtacatcgc gattctgaaa tttggtcaga 1320 gcccgaaaag tttatgccca atagattcttgactagcaaa gcaaatatag atgctcgcgg 1380 tcaaaatttt gaatttatac cgtttggttctgggagacgg tcatgtccag ggttaggttt 1440 tgcgacttta gtgacacatc tgacttttggtcgcttgctt caaggttttg attttagtaa 1500 gccatcaaac acgccaattg acatgacagaaggcgtaggc gttactttgc ctaaggttaa 1560 tcaagttgaa gttctaatta cccctcgtttaccttctaag ctttatttat tttgaaagtg 1620 caaatcatca atcatgggtt gagtaattagtgatact 1657 270 521 PRT NICOTIANATABACUM 270 Met Asp Tyr His Ile SerPhe His Phe Gln Ala Leu Leu Gly Leu Leu 1 5 10 15 Ala Phe Val Phe LeuSer Ile Ile Leu Trp Arg Arg Thr Leu Thr Ser 20 25 30 Arg Lys Leu Ala ProGlu Ile Pro Gly Ala Trp Pro Ile Ile Gly His 35 40 45 Leu Arg Gln Leu SerGly Thr Asp Lys Asn Ile Pro Phe Pro Arg Ile 50 55 60 Leu Gly Ala Leu AlaAsp Lys Tyr Gly Pro Val Phe Thr Leu Arg Ile 65 70 75 80 Gly Met Tyr ProTyr Leu Ile Val Asn Asn Trp Glu Ala Ala Lys Asp 85 90 95 Cys Leu Thr ThrHis Asp Lys Asp Phe Ala Ala Arg Pro Thr Ser Met 100 105 110 Ala Gly GluSer Ile Gly Tyr Lys Tyr Ala Arg Phe Thr Tyr Ala Asn 115 120 125 Phe GlyPro Tyr Tyr Asn Gln Val Arg Lys Leu Ala Leu Gln His Val 130 135 140 LeuSer Ser Thr Lys Leu Glu Lys Met Lys His Ile Arg Val Ser Glu 145 150 155160 Leu Glu Thr Ser Ile Lys Glu Leu Tyr Ser Leu Thr Leu Gly Lys Asn 165170 175 Asn Met Gln Lys Val Asn Ile Ser Lys Trp Phe Glu Gln Leu Thr Leu180 185 190 Asn Ile Ile Val Lys Thr Ile Cys Gly Lys Arg Tyr Ser Asn IleGlu 195 200 205 Glu Asp Glu Glu Ala Gln Arg Phe Arg Lys Ala Phe Lys GlyIle Met 210 215 220 Phe Val Val Gly Gln Ile Val Leu Tyr Asp Ala Ile ProPhe Pro Leu 225 230 235 240 Phe Lys Tyr Phe Asp Phe Gln Gly His Ile GlnLeu Met Asn Lys Ile 245 250 255 Tyr Lys Asp Leu Asp Ser Ile Leu Gln GlyTrp Leu Asp Asp His Met 260 265 270 Met Asn Lys Asp Val Asn Asn Lys AspGln Asp Ala Ile Asp Ala Met 275 280 285 Leu Lys Val Thr Gln Leu Asn GluPhe Lys Ala Tyr Gly Phe Ser Gln 290 295 300 Ala Thr Val Ile Lys Ser ThrVal Leu Ser Leu Ile Leu Asp Gly Asn 305 310 315 320 Asp Thr Thr Ala ValHis Leu Ile Trp Val Met Ser Leu Leu Leu Asn 325 330 335 Asn Pro His ValMet Lys Gln Gly Gln Glu Glu Ile Asp Met Lys Val 340 345 350 Gly Lys GluArg Trp Ile Glu Asp Thr Asp Ile Lys Asn Leu Val Tyr 355 360 365 Leu GlnAla Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Pro Val Pro 370 375 380 PheLeu Leu Pro His Glu Ala Val Gln Asp Cys Lys Val Thr Gly Tyr 385 390 395400 His Ile Pro Lys Gly Thr Arg Leu Tyr Ile Asn Ala Trp Lys Val His 405410 415 Arg Asp Ser Glu Ile Trp Ser Glu Pro Glu Lys Phe Met Pro Asn Arg420 425 430 Phe Leu Thr Ser Lys Ala Asn Ile Asp Ala Arg Gly Gln Asn PheGlu 435 440 445 Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly LeuGly Phe 450 455 460 Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu LeuGln Gly Phe 465 470 475 480 Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile AspMet Thr Glu Gly Val 485 490 495 Gly Val Thr Leu Pro Lys Val Asn Gln ValGlu Val Leu Ile Thr Pro 500 505 510 Arg Leu Pro Ser Lys Leu Tyr Leu Phe515 520 271 1620 DNA NICOTIANATABACUM 271 agagagtgaa aatggacgcactacttcaaa tgacagtaac agcatcttgt gctgccatag 60 taattactct gctggtgtgtatatggagag tgctgaactg gatttggttc agaccaaaga 120 aattggagtt gttgttgagaaaacaaggtt tggaaggaaa ttcttacaag gttttgtatg 180 gggacatgaa agagttttctgggatgatta aggaagcata ctcaaagcct atgagtctat 240 ctgatgatgt agcaccaagactgatgcctt tctttcttga aaccatcaaa aaatatggaa 300 aaagatcctt tatatggtttggtccaagac cactagtatt gattatggat cctgagctta 360 taaaggaagt actctcaaaaatccatctgt atcaaaagcc tggtggaaat ccattagcaa 420 cactattggt acaaggaatagcaacctatg aggaagacaa atgggccaaa catagaaaaa 480 tcatcaatcc cgctttccatctagagaagc taaagcttat gcttccagca tttcgcttaa 540 gctgtagtga gatgctgagcaaatgggaag acattgtttc agctgatagc tcacatgaga 600 tagatgtatg gtctcaccttgagcaattga cttgcgatgt gatctctcgg acagcttttg 660 gcagtagtta tgaagaaggtagaaagattt ttgaacttca aaaggaacaa gctcagtatc 720 ttgtggaagt tttccgctccgtttatatcc caggaaggag atttttgcca acaaagagga 780 atagaagaat gaaggaaataaaaaaggatg tccgggcatc aattaaaggt attattgata 840 aaagattgaa ggcaatgaaagcaggggaca ccaataatga ggatctattg ggtatattac 900 tggaatcgaa tattaaagaaattgaacagc acggaaacaa ggattttgga atgagcattg 960 aagaagtcat tgaagaatgcaagttattct attttgctgg ccaagaaact acatcagtgt 1020 tactcctatg gtctctagtgttgttgagca ggtatcaaga ttggcaggca cgggccagag 1080 aagaaatctt gcaagtctttggcagtcgaa aaccagattt tgacggatta aatcatctaa 1140 aaattgtgac aatgatcttgtacgagtctt taaggctgta tccctcacta ataacactta 1200 cccgccggtg taatgaagacattgtattag gagaactatc tctaccagct ggtgttctag 1260 tctctttgcc attgattttgttgcatcatg atgaagagat atggggtgaa gatgcaaagg 1320 agttcaaacc agagagatttagagaaggaa tatcaagtgc aacaaagggt caactcacat 1380 attttccatt tagctggggtcctagaatat gtattggaca aaattttgcc atgttagaag 1440 caaagatggc tctgtctatgatcctgcaac gcttctcttt tgaactgtct ccgtcttatg 1500 cacatgcccc tcggtccataataaccgttc agcctcagta tggtgctcca cttattttcc 1560 acaaactata attttggtacttctactaat attttagggt ttattcagac tcaaaaaaaa 1620 272 513 PRTNICOTIANATABACUM 272 Met Thr Val Thr Ala Ser Cys Ala Ala Ile Val Ile ThrLeu Leu Val 1 5 10 15 Cys Ile Trp Arg Val Leu Asn Trp Ile Trp Phe ArgPro Lys Lys Leu 20 25 30 Glu Leu Leu Leu Arg Lys Gln Gly Leu Glu Gly AsnSer Tyr Lys Val 35 40 45 Leu Tyr Gly Asp Met Lys Glu Phe Ser Gly Met IleLys Glu Ala Tyr 50 55 60 Ser Lys Pro Met Ser Leu Ser Asp Asp Val Ala ProArg Leu Met Pro 65 70 75 80 Phe Phe Leu Glu Thr Ile Lys Lys Tyr Gly LysArg Ser Phe Ile Trp 85 90 95 Phe Gly Pro Arg Pro Leu Val Leu Ile Met AspPro Glu Leu Ile Lys 100 105 110 Glu Val Leu Ser Lys Ile His Leu Tyr GlnLys Pro Gly Gly Asn Pro 115 120 125 Leu Ala Thr Leu Leu Val Gln Gly IleAla Thr Tyr Glu Glu Asp Lys 130 135 140 Trp Ala Lys His Arg Lys Ile IleAsn Pro Ala Phe His Leu Glu Lys 145 150 155 160 Leu Lys Leu Met Leu ProAla Phe Arg Leu Ser Cys Ser Glu Met Leu 165 170 175 Ser Lys Trp Glu AspIle Val Ser Ala Asp Ser Ser His Glu Ile Asp 180 185 190 Val Trp Ser HisLeu Glu Gln Leu Thr Cys Asp Val Ile Ser Arg Thr 195 200 205 Ala Phe GlySer Ser Tyr Glu Glu Gly Arg Lys Ile Phe Glu Leu Gln 210 215 220 Lys GluGln Ala Gln Tyr Leu Val Glu Val Phe Arg Ser Val Tyr Ile 225 230 235 240Pro Gly Arg Arg Phe Leu Pro Thr Lys Arg Asn Arg Arg Met Lys Glu 245 250255 Ile Lys Lys Asp Val Arg Ala Ser Ile Lys Gly Ile Ile Asp Lys Arg 260265 270 Leu Lys Ala Met Lys Ala Gly Asp Thr Asn Asn Glu Asp Leu Leu Gly275 280 285 Ile Leu Leu Glu Ser Asn Ile Lys Glu Ile Glu Gln His Gly AsnLys 290 295 300 Asp Phe Gly Met Ser Ile Glu Glu Val Ile Glu Glu Cys LysLeu Phe 305 310 315 320 Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu LeuLeu Trp Ser Leu 325 330 335 Val Leu Leu Ser Arg Tyr Gln Asp Trp Gln AlaArg Ala Arg Glu Glu 340 345 350 Ile Leu Gln Val Phe Gly Ser Arg Lys ProAsp Phe Asp Gly Leu Asn 355 360 365 His Leu Lys Ile Val Thr Met Ile LeuTyr Glu Ser Leu Arg Leu Tyr 370 375 380 Pro Ser Leu Ile Thr Leu Thr ArgArg Cys Asn Glu Asp Ile Val Leu 385 390 395 400 Gly Glu Leu Ser Leu ProAla Gly Val Leu Val Ser Leu Pro Leu Ile 405 410 415 Leu Leu His His AspGlu Glu Ile Trp Gly Glu Asp Ala Lys Glu Phe 420 425 430 Lys Pro Glu ArgPhe Arg Glu Gly Ile Ser Ser Ala Thr Lys Gly Gln 435 440 445 Leu Thr TyrPhe Pro Phe Ser Trp Gly Pro Arg Ile Cys Ile Gly Gln 450 455 460 Asn PheAla Met Leu Glu Ala Lys Met Ala Leu Ser Met Ile Leu Gln 465 470 475 480Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Arg Ser 485 490495 Ile Ile Thr Val Gln Pro Gln Tyr Gly Ala Pro Leu Ile Phe His Lys 500505 510 Leu 273 1671 DNA NICOTIANATABACUM 273 tgcaatatag ttttcctagtcagttctagc ctccttttcc ttagaaataa tggattatca 60 tatttctttc cattttcaagctcttttagg gcttttagcc tttgtgttct tgtctattat 120 cttatggaga agaacactcacttcaagaaa attagcccct gaaatcccag gggcatggcc 180 tattataggc catcttcgtcagctgagtgg tactgataag aatatcccat ttccccgaat 240 attgggcgct ttggcagataaatatggacc tgtcttcaca ctgagaatag ggatgtaccc 300 ctatttgatt gtcaacaattgggaagcagc taaggattgt ctcacaacgc atgataagga 360 cttggctgcc cgaccaacttctatggctgg tgaaagcatc gggtacaagt atgcgaggtt 420 tacttatgct aattttggtccttattataa ccaagtgcgc aaactagccc tacaacatgt 480 actctcgagt actaaactcgagaaaatgaa acacatacgt gtttctgaat tggaaactag 540 catcaaagaa ttatattctttgacgctggg caaaaacaac atgcaaaaag tgaatataag 600 taaatggttt gaacaattgactttaaacat aatcgtgaag acaatttgtg gcaagagata 660 tagcaacata gaggaggatgaagaggcaca acgtttcaga aaggcattta agggcatcat 720 gtttgttgta gggcaaattgttttatatga cgcaattcca ttcccattgt tcaaatactt 780 tgatttccaa ggtcatatacaattgatgaa caaaatttat aaagacttag attctattct 840 tcaaggatgg ttggatgatcatatgatgaa caaggatgta aacaataagg atcaagatgc 900 catagatgcc atgcttaaggtaacacaact taatgaattc aaagcctatg gtttttctca 960 ggccactgtg atcaagtcgacagtcttgag tttgatctta gatggaaatg acacaaccgc 1020 tgttcatttg atatgggtaatgtccttatt actgaacaat ccacatgtta tgaaacaagg 1080 ccaagaagag atagacatgaaagtgggtaa agagaggtgg attgaagata ctgacataaa 1140 aaatttagtg taccttcaggctatcgttaa agagacattg cgcttgtatc cacctgttcc 1200 ttttctttta ccacacgaagcagtgcaaga ttgtaaagtg actggttacc acattcctaa 1260 aggtactcgt ctatatatcaatgcgtggaa agtacatcgc gatcctgaaa tttggtcaga 1320 gcccgaaaag tttatgcccaatagattctt gactagcaaa gcaaatatag atgctcgcgg 1380 tcaaaatttt gaatttataccgtttggttc tgggagacgg tcatgtccag ggataggttt 1440 tgcgacttta gtgacacatctgacttttgg tcgcttgctt caaggttttg attttagtaa 1500 gccatcaaac acgccaattgacatgacaga aggcgtaggc gttactttgc ctaaggttaa 1560 tcaagttgaa gttctaattacccctcgttt accttctaag ctttatttat tttgaaggtg 1620 caaatcatca atcatggcttgagtaattag ttatacttta atatgtttct c 1671 274 521 PRT NICOTIANATABACUM 274Met Asp Tyr His Ile Ser Phe His Phe Gln Ala Leu Leu Gly Leu Leu 1 5 1015 Ala Phe Val Phe Leu Ser Ile Ile Leu Trp Arg Arg Thr Leu Thr Ser 20 2530 Arg Lys Leu Ala Pro Glu Ile Pro Gly Ala Trp Pro Ile Ile Gly His 35 4045 Leu Arg Gln Leu Ser Gly Thr Asp Lys Asn Ile Pro Phe Pro Arg Ile 50 5560 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Leu Arg Ile 65 7075 80 Gly Met Tyr Pro Tyr Leu Ile Val Asn Asn Trp Glu Ala Ala Lys Asp 8590 95 Cys Leu Thr Thr His Asp Lys Asp Leu Ala Ala Arg Pro Thr Ser Met100 105 110 Ala Gly Glu Ser Ile Gly Tyr Lys Tyr Ala Arg Phe Thr Tyr AlaAsn 115 120 125 Phe Gly Pro Tyr Tyr Asn Gln Val Arg Lys Leu Ala Leu GlnHis Val 130 135 140 Leu Ser Ser Thr Lys Leu Glu Lys Met Lys His Ile ArgVal Ser Glu 145 150 155 160 Leu Glu Thr Ser Ile Lys Glu Leu Tyr Ser LeuThr Leu Gly Lys Asn 165 170 175 Asn Met Gln Lys Val Asn Ile Ser Lys TrpPhe Glu Gln Leu Thr Leu 180 185 190 Asn Ile Ile Val Lys Thr Ile Cys GlyLys Arg Tyr Ser Asn Ile Glu 195 200 205 Glu Asp Glu Glu Ala Gln Arg PheArg Lys Ala Phe Lys Gly Ile Met 210 215 220 Phe Val Val Gly Gln Ile ValLeu Tyr Asp Ala Ile Pro Phe Pro Leu 225 230 235 240 Phe Lys Tyr Phe AspPhe Gln Gly His Ile Gln Leu Met Asn Lys Ile 245 250 255 Tyr Lys Asp LeuAsp Ser Ile Leu Gln Gly Trp Leu Asp Asp His Met 260 265 270 Met Asn LysAsp Val Asn Asn Lys Asp Gln Asp Ala Ile Asp Ala Met 275 280 285 Leu LysVal Thr Gln Leu Asn Glu Phe Lys Ala Tyr Gly Phe Ser Gln 290 295 300 AlaThr Val Ile Lys Ser Thr Val Leu Ser Leu Ile Leu Asp Gly Asn 305 310 315320 Asp Thr Thr Ala Val His Leu Ile Trp Val Met Ser Leu Leu Leu Asn 325330 335 Asn Pro His Val Met Lys Gln Gly Gln Glu Glu Ile Asp Met Lys Val340 345 350 Gly Lys Glu Arg Trp Ile Glu Asp Thr Asp Ile Lys Asn Leu ValTyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro ProVal Pro 370 375 380 Phe Leu Leu Pro His Glu Ala Val Gln Asp Cys Lys ValThr Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Tyr Ile AsnAla Trp Lys Val His 405 410 415 Arg Asp Pro Glu Ile Trp Ser Glu Pro GluLys Phe Met Pro Asn Arg 420 425 430 Phe Leu Thr Ser Lys Ala Asn Ile AspAla Arg Gly Gln Asn Phe Glu 435 440 445 Phe Ile Pro Phe Gly Ser Gly ArgArg Ser Cys Pro Gly Ile Gly Phe 450 455 460 Ala Thr Leu Val Thr His LeuThr Phe Gly Arg Leu Leu Gln Gly Phe 465 470 475 480 Asp Phe Ser Lys ProSer Asn Thr Pro Ile Asp Met Thr Glu Gly Val 485 490 495 Gly Val Thr LeuPro Lys Val Asn Gln Val Glu Val Leu Ile Thr Pro 500 505 510 Arg Leu ProSer Lys Leu Tyr Leu Phe 515 520 275 1618 DNA NICOTIANATABACUM 275tttgattcaa ccatggagaa ccaatactcc tactcattct cttcctactt ctacttagct 60atagtactgt ttcttcttcc aattttggtc aaatatttct tccatcggag aagaaattta 120cctccaagtc cattttctct tccaataatt ggtcaccttt accttctcaa gaaaactctc 180catctcactc taacatcctt atcagctaaa tatggtcctg ttttatacct caaattgggc 240tctatgcctg tgattgttgt gtcctcacca tctgctgttg aagaatgttt aaccaagaat 300gatatcatat tcgcaaatag gcccaagacc gtggctggtg acaagtttac ctacaattat 360actgtttatg tttgggcacc ctatggccaa ctttggagaa ttcttcgccg attaactgtc 420gttgaactct tctcttcaca tagcctacag aaaacttcta tccttagaga tcaagaagtt 480gcaatattta tccgttcgtt atacaaattc tcaaaggata gtagcaaaaa agtcgatttg 540accaactggt cttttacttt ggttttcaat cttatgacca aaattattgc tgggagacat 600attgtgaagg aggaagatgc tggcaaggaa aagggcattg aaattattga aaaacttaga 660gggactttct tagtaactac atcattcttg aatatgtgtg atttcttgcc agtattcagg 720tgggttggtt acaaagggca ggagaagaag atggcctcaa ttcacaatag aagaaatgaa 780ttcttgaaca gcttgcttga tgaatttcga cacaagaaaa gtagtgcttc acaatctaac 840acaactgttg gaaacatgga gaagaaaacc acactgattg aaaagctctt gtctcttcaa 900gaatcagagc ctgaattcta cactgatgat atcatcaaaa gtattatgct ggtagttttt 960gttgcaggaa cagagacctc atcaacaacc atccaatggg taatgaggct tcttgtagct 1020caccctgagg cattgtataa gctacgagct gacattgaca gtaaagttgg gaataagcgc 1080ttgctgaatg aatcagacct caacaagctt ccgtatttgc attgtgttgt taatgagaca 1140atgagattat acactccgat accactttta ttgcctcatt attcaactaa agattgtatt 1200gtggaaggat atgatgtacc aaaacataca atgttgtttg tcaacgcttg ggccattcac 1260agggatccca aggtatggga ggagcctgac aagttcaagc cagagagatt tgaggcaaca 1320gaaggggaaa cagaaaggtt caattacaag cttgtaccat ttggaatggg gagaagagcg 1380tgccctggag ctgatatggg gttgcgagca gtttctttgg cattaggtgc acttattcaa 1440tgctttgact ggcaaattga ggaagcggaa agcttggagg aaagctataa ttctagaatg 1500actatgcaga acaagccttt gaaggttgtc tgcactccac gcgaagatct tggccagctt 1560ctatcccaac tctaaggcaa tttatcaatg ccaaacgtaa tcttcatcta ccactatg 1618 276520 PRT NICOTIANATABACUM 276 Met Glu Asn Gln Tyr Ser Tyr Ser Phe Ser SerTyr Phe Tyr Leu Ala 1 5 10 15 Ile Val Leu Phe Leu Leu Pro Ile Leu ValLys Tyr Phe Phe His Arg 20 25 30 Arg Arg Asn Leu Pro Pro Ser Pro Phe SerLeu Pro Ile Ile Gly His 35 40 45 Leu Tyr Leu Leu Lys Lys Thr Leu His LeuThr Leu Thr Ser Leu Ser 50 55 60 Ala Lys Tyr Gly Pro Val Leu Tyr Leu LysLeu Gly Ser Met Pro Val 65 70 75 80 Ile Val Val Ser Ser Pro Ser Ala ValGlu Glu Cys Leu Thr Lys Asn 85 90 95 Asp Ile Ile Phe Ala Asn Arg Pro LysThr Val Ala Gly Asp Lys Phe 100 105 110 Thr Tyr Asn Tyr Thr Val Tyr ValTrp Ala Pro Tyr Gly Gln Leu Trp 115 120 125 Arg Ile Leu Arg Arg Leu ThrVal Val Glu Leu Phe Ser Ser His Ser 130 135 140 Leu Gln Lys Thr Ser IleLeu Arg Asp Gln Glu Val Ala Ile Phe Ile 145 150 155 160 Arg Ser Leu TyrLys Phe Ser Lys Asp Ser Ser Lys Lys Val Asp Leu 165 170 175 Thr Asn TrpSer Phe Thr Leu Val Phe Asn Leu Met Thr Lys Ile Ile 180 185 190 Ala GlyArg His Ile Val Lys Glu Glu Asp Ala Gly Lys Glu Lys Gly 195 200 205 IleGlu Ile Ile Glu Lys Leu Arg Gly Thr Phe Leu Val Thr Thr Ser 210 215 220Phe Leu Asn Met Cys Asp Phe Leu Pro Val Phe Arg Trp Val Gly Tyr 225 230235 240 Lys Gly Gln Glu Lys Lys Met Ala Ser Ile His Asn Arg Arg Asn Glu245 250 255 Phe Leu Asn Ser Leu Leu Asp Glu Phe Arg His Lys Lys Ser SerAla 260 265 270 Ser Gln Ser Asn Thr Thr Val Gly Asn Met Glu Lys Lys ThrThr Leu 275 280 285 Ile Glu Lys Leu Leu Ser Leu Gln Glu Ser Glu Pro GluPhe Tyr Thr 290 295 300 Asp Asp Ile Ile Lys Ser Ile Met Leu Val Val PheVal Ala Gly Thr 305 310 315 320 Glu Thr Ser Ser Thr Thr Ile Gln Trp ValMet Arg Leu Leu Val Ala 325 330 335 His Pro Glu Ala Leu Tyr Lys Leu ArgAla Asp Ile Asp Ser Lys Val 340 345 350 Gly Asn Lys Arg Leu Leu Asn GluSer Asp Leu Asn Lys Leu Pro Tyr 355 360 365 Leu His Cys Val Val Asn GluThr Met Arg Leu Tyr Thr Pro Ile Pro 370 375 380 Leu Leu Leu Pro His TyrSer Thr Lys Asp Cys Ile Val Glu Gly Tyr 385 390 395 400 Asp Val Pro LysHis Thr Met Leu Phe Val Asn Ala Trp Ala Ile His 405 410 415 Arg Asp ProLys Val Trp Glu Glu Pro Asp Lys Phe Lys Pro Glu Arg 420 425 430 Phe GluAla Thr Glu Gly Glu Thr Glu Arg Phe Asn Tyr Lys Leu Val 435 440 445 ProPhe Gly Met Gly Arg Arg Ala Cys Pro Gly Ala Asp Met Gly Leu 450 455 460Arg Ala Val Ser Leu Ala Leu Gly Ala Leu Ile Gln Cys Phe Asp Trp 465 470475 480 Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser Tyr Asn Ser Arg Met485 490 495 Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys Thr Pro Arg GluAsp 500 505 510 Leu Gly Gln Leu Leu Ser Gln Leu 515 520 277 1676 DNANICOTIANATABACUM 277 cctttttaag atgtatttaa gatttaagat ttaagatgaagcaactgagg taagtccttt 60 caaggagtag ttgtcacttc tgagaatgga gatgatgtacagcataatag cagcagccag 120 tattgcaatt atcttggtat atacatggaa agtgttgaattgggcttggt ttgggccgaa 180 gaaaatggag aaatgcttaa gacagagggg tctcaagggaaatccttata agctactcta 240 tggagatcta aacgaactga caaaaagcat aatagaagccaagtctaagc ccatcaattt 300 ctctgatgat attgctcaaa ggctcatccc tttttttcttgacgccatca acaaaaatgg 360 taaaaactcc ttcgtctggc ttggaccgta tccaatagtgttgatcacgg atcctgagca 420 tttaaaggag attttcacaa agaattatgt gtatcaaaagcaaactcatc ccaatccata 480 cgccaagcta ttagctcacg gtcttgtcag ccttgaggaagacaaatggg ccaaacacag 540 aaaaatcatt agtcctgcct tccatgtcga gaagctaaagcatatgctgc ctgcatttta 600 tctgagttgt agtgaaatga taagcaaatg ggaggaggttgttccaaaag aaacatcatt 660 cgagctcgat gtatggccag accttcaaat aatgaccagtgaagtcattt ctcgcactgc 720 atttgggagt agctatgaag aaggaagaat agtatttgaacttcagaaag aacaagctga 780 gtatgtaatg gacataggac gttcaattta tataccaggatcaaggttct tgcctactaa 840 aaggaacaaa agaatgctgg aaattgaaaa gcaagtgcaaacaacaatta ggcgtatcat 900 cgacaaaaga ttgaaggcaa tggaagaagg ggagactagtaaagatgact tattaggcat 960 attacttgaa tccaatttga aagaaattga acttcatggaagaaatgact tgggaataac 1020 aacgtcagaa gtgattgaag agtgcaagtt attctattttgccggccaag agaccacttc 1080 agtgttgctt gtttggacaa tgattttgtt gtgcttacatccagagtggc aagtacgtgc 1140 cagaaaggaa gtgttgcaga tctttggaaa tgataaaccagatttggaag gactaagtcg 1200 cttgaaaatt gtaacaatga tcttgtacga gacgttacgcctattccccc cattaccagc 1260 atttggtaga aggaacaaag aagaagtcaa attaggggagctacatctac cggctggagt 1320 gttactcgtt ataccagcaa tcttagtaca ttatgataaggaaatatggg gtgaagatgc 1380 aaaggaattc aaaccagaaa gattcagtga aggagtgtcaaaggcaacaa atggacaagt 1440 ctcatttata ccatttagct ggggacctcg tgtttgcattggacaaaact tcgcaatgat 1500 ggaagcaaaa atggcagtaa ctatgatact acaaaaattctcctttgaac tatccccttc 1560 ttatacacat gctccatttg caattgtgac tattcatccccagtatggtg ctcctctgct 1620 tatgcgcaga ctttaaaaca tatgttgctg atatttaagatcagtggcgt tttatt 1676 278 516 PRT NICOTIANATABACUM 278 Met Glu Met MetTyr Ser Ile Ile Ala Ala Ala Ser Ile Ala Ile Ile 1 5 10 15 Leu Val TyrThr Trp Lys Val Leu Asn Trp Ala Trp Phe Gly Pro Lys 20 25 30 Lys Met GluLys Cys Leu Arg Gln Arg Gly Leu Lys Gly Asn Pro Tyr 35 40 45 Lys Leu LeuTyr Gly Asp Leu Asn Glu Leu Thr Lys Ser Ile Ile Glu 50 55 60 Ala Lys SerLys Pro Ile Asn Phe Ser Asp Asp Ile Ala Gln Arg Leu 65 70 75 80 Ile ProPhe Phe Leu Asp Ala Ile Asn Lys Asn Gly Lys Asn Ser Phe 85 90 95 Val TrpLeu Gly Pro Tyr Pro Ile Val Leu Ile Thr Asp Pro Glu His 100 105 110 LeuLys Glu Ile Phe Thr Lys Asn Tyr Val Tyr Gln Lys Gln Thr His 115 120 125Pro Asn Pro Tyr Ala Lys Leu Leu Ala His Gly Leu Val Ser Leu Glu 130 135140 Glu Asp Lys Trp Ala Lys His Arg Lys Ile Ile Ser Pro Ala Phe His 145150 155 160 Val Glu Lys Leu Lys His Met Leu Pro Ala Phe Tyr Leu Ser CysSer 165 170 175 Glu Met Ile Ser Lys Trp Glu Glu Val Val Pro Lys Glu ThrSer Phe 180 185 190 Glu Leu Asp Val Trp Pro Asp Leu Gln Ile Met Thr SerGlu Val Ile 195 200 205 Ser Arg Thr Ala Phe Gly Ser Ser Tyr Glu Glu GlyArg Ile Val Phe 210 215 220 Glu Leu Gln Lys Glu Gln Ala Glu Tyr Val MetAsp Ile Gly Arg Ser 225 230 235 240 Ile Tyr Ile Pro Gly Ser Arg Phe LeuPro Thr Lys Arg Asn Lys Arg 245 250 255 Met Leu Glu Ile Glu Lys Gln ValGln Thr Thr Ile Arg Arg Ile Ile 260 265 270 Asp Lys Arg Leu Lys Ala MetGlu Glu Gly Glu Thr Ser Lys Asp Asp 275 280 285 Leu Leu Gly Ile Leu LeuGlu Ser Asn Leu Lys Glu Ile Glu Leu His 290 295 300 Gly Arg Asn Asp LeuGly Ile Thr Thr Ser Glu Val Ile Glu Glu Cys 305 310 315 320 Lys Leu PheTyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu Val 325 330 335 Trp ThrMet Ile Leu Leu Cys Leu His Pro Glu Trp Gln Val Arg Ala 340 345 350 ArgLys Glu Val Leu Gln Ile Phe Gly Asn Asp Lys Pro Asp Leu Glu 355 360 365Gly Leu Ser Arg Leu Lys Ile Val Thr Met Ile Leu Tyr Glu Thr Leu 370 375380 Arg Leu Phe Pro Pro Leu Pro Ala Phe Gly Arg Arg Asn Lys Glu Glu 385390 395 400 Val Lys Leu Gly Glu Leu His Leu Pro Ala Gly Val Leu Leu ValIle 405 410 415 Pro Ala Ile Leu Val His Tyr Asp Lys Glu Ile Trp Gly GluAsp Ala 420 425 430 Lys Glu Phe Lys Pro Glu Arg Phe Ser Glu Gly Val SerLys Ala Thr 435 440 445 Asn Gly Gln Val Ser Phe Ile Pro Phe Ser Trp GlyPro Arg Val Cys 450 455 460 Ile Gly Gln Asn Phe Ala Met Met Glu Ala LysMet Ala Val Thr Met 465 470 475 480 Ile Leu Gln Lys Phe Ser Phe Glu LeuSer Pro Ser Tyr Thr His Ala 485 490 495 Pro Phe Ala Ile Val Thr Ile HisPro Gln Tyr Gly Ala Pro Leu Leu 500 505 510 Met Arg Arg Leu 515 279 1577DNA NICOTIANATABACUM 279 ctaaatttca tataccttta gtactcttga aattttcaaataatggttta tcttctttct 60 cccatagaag ccattgtagg atttgtaacc ttttcatttctattctactt tctatggacc 120 aaaaaacaat caaaaatctt aaacccacta cctccaaaaatcccaggtgg atggccagta 180 atcggccatc tcttttattt caagaacaat ggcgatgaagatcgccattt ttctcaaaaa 240 ctcggtgact tagctgacaa atatggtccc gtcttcactttccggttagg gtttcgccgt 300 ttcttggcgg tgagtagtta tgaagctatg aaagaatgcttcactaccaa tgatatccat 360 ttcgccgatc ggccatcttt actctacgga gaatacctttgctataataa tgccatgctt 420 gctgttgcca aatatggccc ttactggaaa aaaaatcgaaagttagtcaa tcaagaagtt 480 ctctccgtta gtcggctcga aaaattcaaa catgttagattttctataat tcagaaaaat 540 attaaacaat tgtataattg tgattcacca atggtgaagataaaccttag tgattggata 600 gataaattga cattcgacat cattttgaaa atggttgttgggaagaacta taataatgga 660 catggagaaa tactcaaagt tgcttttcag aaattcatggttcaagctat ggagatggag 720 ctctatgatg tttttcacat tccatttttc aagtggttggatcttacagg gaatattaag 780 gctatgaaac aaactttcaa agacattgat aatattatccaaggttggtt agatgagcac 840 attaagaaga gagaaacaaa ggatgttgga ggtgaaaacgaacaagattt tatagatgtg 900 gtgctttcca agatgagcga cgaacatctt ggcgagggttactctcatga cacaaccatc 960 aaagcaactg tattcacttt ggtcttggat gcaacagacacacttgcact tcatataaag 1020 tgggtaatgg cgttaatgat aaacaataag catgtcatgaagaaagcaca agaagagatg 1080 gacacaattg ttggtagaga tagatgggta gaagagagtgatatcaagaa tttggtgtat 1140 ctccaagcaa ttgttaaaga agtattacga ttacatccacctgcaccttt gtcagtgcaa 1200 cacctatctg tggaagattg tgttgtcaat gggtaccatattcctaaggg gactgcacta 1260 cttaccaata ttatgaaact acagcgagat cctcaaacatggccaaatcc tgataaattc 1320 gatccagaga gattcctgac gactcatgct actattgactaccgcgggca gcactatgag 1380 ttgatcccct ttggtacggg gagacgagct tgtcccgcgatgaattattc attgcaagtg 1440 gaacaccttt caattgctca tatgatccaa ggtttcagttttgcaactac gaccaatgag 1500 cctttggata tgaaacaagg tgtgggttta actttaccaaagaagactga tgttgaagtt 1560 ctaattaccc ctcgttt 1577 280 511 PRTNICOTIANATABACUM 280 Met Val Tyr Leu Leu Ser Pro Ile Glu Ala Ile Val GlyPhe Val Thr 1 5 10 15 Phe Ser Phe Leu Phe Tyr Phe Leu Trp Thr Lys LysGln Ser Lys Ile 20 25 30 Leu Asn Pro Leu Pro Pro Lys Ile Pro Gly Gly TrpPro Val Ile Gly 35 40 45 His Leu Phe Tyr Phe Lys Asn Asn Gly Asp Glu AspArg His Phe Ser 50 55 60 Gln Lys Leu Gly Asp Leu Ala Asp Lys Tyr Gly ProVal Phe Thr Phe 65 70 75 80 Arg Leu Gly Phe Arg Arg Phe Leu Ala Val SerSer Tyr Glu Ala Met 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Ile His PheAla Asp Arg Pro Ser 100 105 110 Leu Leu Tyr Gly Glu Tyr Leu Cys Tyr AsnAsn Ala Met Leu Ala Val 115 120 125 Ala Lys Tyr Gly Pro Tyr Trp Lys LysAsn Arg Lys Leu Val Asn Gln 130 135 140 Glu Val Leu Ser Val Ser Arg LeuGlu Lys Phe Lys His Val Arg Phe 145 150 155 160 Ser Ile Ile Gln Lys AsnIle Lys Gln Leu Tyr Asn Cys Asp Ser Pro 165 170 175 Met Val Lys Ile AsnLeu Ser Asp Trp Ile Asp Lys Leu Thr Phe Asp 180 185 190 Ile Ile Leu LysMet Val Val Gly Lys Asn Tyr Asn Asn Gly His Gly 195 200 205 Glu Ile LeuLys Val Ala Phe Gln Lys Phe Met Val Gln Ala Met Glu 210 215 220 Met GluLeu Tyr Asp Val Phe His Ile Pro Phe Phe Lys Trp Leu Asp 225 230 235 240Leu Thr Gly Asn Ile Lys Ala Met Lys Gln Thr Phe Lys Asp Ile Asp 245 250255 Asn Ile Ile Gln Gly Trp Leu Asp Glu His Ile Lys Lys Arg Glu Thr 260265 270 Lys Asp Val Gly Gly Glu Asn Glu Gln Asp Phe Ile Asp Val Val Leu275 280 285 Ser Lys Met Ser Asp Glu His Leu Gly Glu Gly Tyr Ser His AspThr 290 295 300 Thr Ile Lys Ala Thr Val Phe Thr Leu Val Leu Asp Ala ThrAsp Thr 305 310 315 320 Leu Ala Leu His Ile Lys Trp Val Met Ala Leu MetIle Asn Asn Lys 325 330 335 His Val Met Lys Lys Ala Gln Glu Glu Met AspThr Ile Val Gly Arg 340 345 350 Asp Arg Trp Val Glu Glu Ser Asp Ile LysAsn Leu Val Tyr Leu Gln 355 360 365 Ala Ile Val Lys Glu Val Leu Arg LeuHis Pro Pro Ala Pro Leu Ser 370 375 380 Val Gln His Leu Ser Val Glu AspCys Val Val Asn Gly Tyr His Ile 385 390 395 400 Pro Lys Gly Thr Ala LeuLeu Thr Asn Ile Met Lys Leu Gln Arg Asp 405 410 415 Pro Gln Thr Trp ProAsn Pro Asp Lys Phe Asp Pro Glu Arg Phe Leu 420 425 430 Thr Thr His AlaThr Ile Asp Tyr Arg Gly Gln His Tyr Glu Leu Ile 435 440 445 Pro Phe GlyThr Gly Arg Arg Ala Cys Pro Ala Met Asn Tyr Ser Leu 450 455 460 Gln ValGlu His Leu Ser Ile Ala His Met Ile Gln Gly Phe Ser Phe 465 470 475 480Ala Thr Thr Thr Asn Glu Pro Leu Asp Met Lys Gln Gly Val Gly Leu 485 490495 Thr Leu Pro Lys Lys Thr Asp Val Glu Val Leu Ile Thr Pro Arg 500 505510 281 1434 DNA NICOTIANATABACUM misc_feature (564)..(564) n is a, c,g, or t 281 ctttttctcc ccaaaaaaga gctcatttcc cttgtcccca aaaatggatcttctcttact 60 agagaagacc ttaattggtc tcttctttgc cattttaatc gctgtaattgtctctagact 120 tcgttcaaag cgttttaagc ttcccccagg accaatccca gtaccagtttttggtaattg 180 gcttcaagtt ggtgatgatt taaaccacag aaatcttact gattttgccaaaaaatttgg 240 tgatcttttc ttgttaagaa tgggccagcg taatttagtt gttgtgtcatctcctgaatt 300 agctaaagaa gttttacaca cacaaggtgt tgaatttggt tcaagaacaagaaatgttgt 360 atttgatatt tttactggaa aaggtcaaga tatggttttt actgtatatggtgaacactg 420 gagaaaaatg aggagaatta tgactgtacc attttttact aataaagttgtgcagcaata 480 tagagggggg tgggagtttg aagtggcaag tgtaattgag gatgtgaagaaaaatcctga 540 atctgctact aatgggattg tatnaaggag gagattacaa ttgatgatgtataataatat 600 gtttaggatt atgtttgata ggagatttga gagtgaagat gatcctttgtttgttaagct 660 taaggctttg aatggtgaaa ggagtagatt ggctcagagt tttgagtataattatggtga 720 ttttattccc attttgaggc cttttttgag aggttatttg aagatctgtaaagaagttaa 780 ggagaagagg ctgcagcttt tcaaagatta ctttgttgat gaaagaaagaagctttcaaa 840 taccaagagc ttggacagca atgctctgaa atgtgcgatt gatcacattcttgaggctca 900 acagaagggg gagatcaatg aggacaacgt tctttacatt gttgaaaacatcaatgttgc 960 tgctatagaa accacattat ggtcaattga gtggggtatc gccgagttagtcaaccaccc 1020 tcacatccaa aagaaactcc gcgacgagat tgacacagtt cttggcccaggagtgcaagt 1080 gactgaacca gacacccaca agcttccata ccttcaggct gtgatcaaggagacgcttcg 1140 tctccgtatg gcaattcctc tattagtccc acacatgaac cttcacgatgcaaagcttgg 1200 cgggtttgat attccagcag agagcaaaat cttggttaac gcttggtggctagctaacaa 1260 cccggctcat tggaagaaac ccgaagagtt cagacccgag aggttcttcgaagaggagaa 1320 gcacgttgag gccaatggca atgacttcag atatcttccg tttggcgttggtaggaggag 1380 ttgccctgga actatacttg cattgccaat tcttggcatt actttgggacgttt 1434 282 463 PRT NICOTIANATABACUM 282 Met Asp Leu Leu Leu Leu GluLys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu Ile Ala Val IleVal Ser Arg Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro Pro Gly Pro IlePro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly Asp Asp Leu AsnHis Arg Asn Leu Thr Asp Phe Ala Lys Lys 50 55 60 Phe Gly Asp Leu Phe LeuLeu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val Ser Ser Pro GluLeu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu Phe Gly Ser ArgThr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 Lys Gly Gln AspMet Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125 Met Arg ArgIle Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135 140 Gln TyrArg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145 150 155 160Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg 165 170175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp 180185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr AsnTyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly TyrLeu Lys 225 230 235 240 Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Gln LeuPhe Lys Asp Tyr 245 250 255 Phe Val Asp Glu Arg Lys Lys Leu Ser Asn ThrLys Ser Leu Asp Ser 260 265 270 Asn Ala Leu Lys Cys Ala Ile Asp His IleLeu Glu Ala Gln Gln Lys 275 280 285 Gly Glu Ile Asn Glu Asp Asn Val LeuTyr Ile Val Glu Asn Ile Asn 290 295 300 Val Ala Ala Ile Glu Thr Thr LeuTrp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320 Glu Leu Val Asn His ProHis Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330 335 Asp Thr Val Leu GlyPro Gly Val Gln Val Thr Glu Pro Asp Thr His 340 345 350 Lys Leu Pro TyrLeu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg 355 360 365 Met Ala IlePro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys 370 375 380 Leu GlyGly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala 385 390 395 400Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe 405 410415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly 420425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro435 440 445 Gly Thr Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg450 455 460 283 1723 DNA NICOTIANATABACUM 283 ccaaattaga gcaagaaattaacaagtcta gttaccttct ccctttttaa gagtatttaa 60 gatttaagat ttaagatgaagcaactgagg taagtccttt caaggagtag ttgtcacttc 120 tgagaatgga gatgatgtacagcataatag cagcagccag tattgcaatt atcttggtat 180 atacatggaa agtgttgaattgggcttggt ttgggccaaa gaaaatggag aaatgcttaa 240 gacagagggg tctcaagggaaatccttata agctactcta tggagatcta aacgaactga 300 caaaaagcat aatagaagccaagtctaagc ccatcaattt ctctgatgat attgctcaaa 360 ggctcatccc tttttttcttgacgccatca acaaaaatgg taaaaactcc ttcgtctggc 420 ttggaccgta tccaatagtgttgatcacgg atcctgagca tttaaaggag attttcacaa 480 agaattatgt gtatcaaaagcaaactcatc ccaatccata cgccaagcta ttagctcacg 540 gtcttgtcag ccttgaggaagacaaatggg ccaaacacag aaaaatcatt agtcctgcct 600 tccatgtcga gaagctaaagcatatgctgc ctgcatttta tctgagttgt agtgaaatga 660 taagcaaatg ggaggaggttgttccaaaag aaacatcatt cgagctcgat gtatggccag 720 accttcaaat aatgaccagtgaagtcattt ctcgcactgc atttgggagt agctatgaag 780 aaggaagaat agtatttgaacttcagaaag aacaagctga gtatgtaatg gacataggac 840 gttcaattta tataccaggatcaaggttct tgcctactaa aaggaacaaa agaatgctgg 900 aaattgaaaa gcaagtgcaaacaacaatta ggcgtatcat cgacaaaaga ttgaaggcaa 960 tggaagaagg ggagactagtaaagatgact tattaggcat attacttgaa tccaatttga 1020 aagaaattga acttcatggaagaaatgact tgggaataac aacatcagaa gtgattgaag 1080 agtgcaagtt aatctattttgccggccaag agaccacttc agtgttgctt gtttggacaa 1140 tgattttgtt gtgcttacatccagagtggc aagtacgtgc cagaaaggaa gtgttgcaga 1200 cctttggaaa tgataaaccagatttggaag gactaagtcg cttgaaaatt gtaacaatga 1260 tcttgtacga gacgttacgcctattccccc cattaccagc atttggtaga aggaacaaag 1320 aagaagtcaa attaggggagctacatctac cggctggagt gttactcgtt ataccagcaa 1380 tcttagtaca ttatgataaggaaatatggg gtgaagatgc aaaggaattc aaaccagaaa 1440 gattcagtga aggagtgtcaaaggcaacaa atggacaagt ctcatttata ccatttagct 1500 agggacctcg tgtttgcattggacaaaact tcgcaatgat ggaagcaaaa atggcagtaa 1560 ctatgatact acaaaaattctcctttgaac tatccccttc ttatacacat gctccatttg 1620 caattgtgac tattcatccccagtatggtg ctcctctgct tatgcgcaga ctttaaaaca 1680 tatgttgctg atatttaagatcagtggcgt tttattctcc atg 1723 284 458 PRT NICOTIANATABACUM 284 Met GluMet Met Tyr Ser Ile Ile Ala Ala Ala Ser Ile Ala Ile Ile 1 5 10 15 LeuVal Tyr Thr Trp Lys Val Leu Asn Trp Ala Trp Phe Gly Pro Lys 20 25 30 LysMet Glu Lys Cys Leu Arg Gln Arg Gly Leu Lys Gly Asn Pro Tyr 35 40 45 LysLeu Leu Tyr Gly Asp Leu Asn Glu Leu Thr Lys Ser Ile Ile Glu 50 55 60 AlaLys Ser Lys Pro Ile Asn Phe Ser Asp Asp Ile Ala Gln Arg Leu 65 70 75 80Ile Pro Phe Phe Leu Asp Ala Ile Asn Lys Asn Gly Lys Asn Ser Phe 85 90 95Val Trp Leu Gly Pro Tyr Pro Ile Val Leu Ile Thr Asp Pro Glu His 100 105110 Leu Lys Glu Ile Phe Thr Lys Asn Tyr Val Tyr Gln Lys Gln Thr His 115120 125 Pro Asn Pro Tyr Ala Lys Leu Leu Ala His Gly Leu Val Ser Leu Glu130 135 140 Glu Asp Lys Trp Ala Lys His Arg Lys Ile Ile Ser Pro Ala PheHis 145 150 155 160 Val Glu Lys Leu Lys His Met Leu Pro Ala Phe Tyr LeuSer Cys Ser 165 170 175 Glu Met Ile Ser Lys Trp Glu Glu Val Val Pro LysGlu Thr Ser Phe 180 185 190 Glu Leu Asp Val Trp Pro Asp Leu Gln Ile MetThr Ser Glu Val Ile 195 200 205 Ser Arg Thr Ala Phe Gly Ser Ser Tyr GluGlu Gly Arg Ile Val Phe 210 215 220 Glu Leu Gln Lys Glu Gln Ala Glu TyrVal Met Asp Ile Gly Arg Ser 225 230 235 240 Ile Tyr Ile Pro Gly Ser ArgPhe Leu Pro Thr Lys Arg Asn Lys Arg 245 250 255 Met Leu Glu Ile Glu LysGln Val Gln Thr Thr Ile Arg Arg Ile Ile 260 265 270 Asp Lys Arg Leu LysAla Met Glu Glu Gly Glu Thr Ser Lys Asp Asp 275 280 285 Leu Leu Gly IleLeu Leu Glu Ser Asn Leu Lys Glu Ile Glu Leu His 290 295 300 Gly Arg AsnAsp Leu Gly Ile Thr Thr Ser Glu Val Ile Glu Glu Cys 305 310 315 320 LysLeu Ile Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu Val 325 330 335Trp Thr Met Ile Leu Leu Cys Leu His Pro Glu Trp Gln Val Arg Ala 340 345350 Arg Lys Glu Val Leu Gln Thr Phe Gly Asn Asp Lys Pro Asp Leu Glu 355360 365 Gly Leu Ser Arg Leu Lys Ile Val Thr Met Ile Leu Tyr Glu Thr Leu370 375 380 Arg Leu Phe Pro Pro Leu Pro Ala Phe Gly Arg Arg Asn Lys GluGlu 385 390 395 400 Val Lys Leu Gly Glu Leu His Leu Pro Ala Gly Val LeuLeu Val Ile 405 410 415 Pro Ala Ile Leu Val His Tyr Asp Lys Glu Ile TrpGly Glu Asp Ala 420 425 430 Lys Glu Phe Lys Pro Glu Arg Phe Ser Glu GlyVal Ser Lys Ala Thr 435 440 445 Asn Gly Gln Val Ser Phe Ile Pro Phe Ser450 455 285 1717 DNA NICOTIANATABACUM 285 tttgcctttg ctcgtcattgatgacgactt cattttgttt tcttccccac gaaaatggta 60 gatatgatat ggagggacgtagggaagaat tactgggaca aacctagtga gtgaaaatgg 120 aaacagttga aatgatagtaaaagtatctt gtgctgccat agtaattact ctgttggtgt 180 gtctatggag agtgctgaattgggtttggt tcagaccaaa gaaattagag aagttgttga 240 gaaaacaggt tttgtatggggacatgaaag agttttctgg gatgattaag gaagcatact 300 caaagcctat gagtctgtctgatgatgtag caccacgaat gatgcctttc tttcttgaaa 360 ccatcaagaa atatggaaaaagatccttta tatggttcgg tccaagacca ctagtattga 420 tcatggatcc tgagcttataaaggaagtac tctccaaaat ctatctttat caaaagcccg 480 gtggaaatcc attagcaacactattggtac aaggattagc aacctatgag gaagacaaat 540 gggccaaaca tagaaaaatcatcaatcccg ctttccatct agagaagcta aagcatatgc 600 ttccagcttt tcgcttgagctgtagtgaga tgctgagcaa atgggaagac attgtttcag 660 ctgaaggctc acatgagatagatgtatggc ctaaccttga gcaattgagt tgcgatgtga 720 tctctcggac agcttttggcaatagttatg aagaaggtag aaagattttt gaacttcaaa 780 aggaacaaac tcagcatcttgtggaagctt tccgctctgt ttatatccca ggaaggagat 840 ttttgccaac aaagaggaatagaagaatga aggaaataaa aaaggaggtt cgagcgtcaa 900 ttaaaggtat tattgataaaagattgaagg caatgaaagc aggggacacc aataatgagg 960 atctattggg atattgctggaatcaaattt taaagaaatt gaacagcgcg gaaacaagga 1020 ttttggaatg agcattgaagatgtcattga agaatgcaag ttattctatt ttgctggcca 1080 agaaactaca tcagtgttgctcctatggtc tctagtgtcg ttgagcaggt atcaagattg 1140 gcagacacgg gccagagaagaagtcttgca tgtctttggg agtcggaaac cagattttga 1200 tgaattaaat catctaaaagttgtgacaat gatcatgtac gagtctttaa ggctatatcc 1260 ctcactaata acacttacccgccggtgtaa tgaagacatt gtattaggag aactatctct 1320 accagctggt gtcctagtctctttgccaat gattttgttg catcatgatg aagagatatg 1380 gggtgaagat gcaaaggagttcaaaccaga gagatttaga gaaggattgt caagtgcaac 1440 aaagggtcaa cttacatattttccatttgg ctggggtcct agaatatgta ttggacaaaa 1500 ttttgccatg ttagaagcaaagatggctct gtctatgatc ctgcaacgct tctcttttga 1560 actgtctccg tcttatgcacatgcccctca gtccatatta accgttcagc ctcaatatgg 1620 tgctccactt attttccacaagctataatt tggtacttgt gaaaggtgtc ttgtacaata 1680 tgttagtaga gtttattcagacttagatac atgcttc 1717 286 304 PRT NICOTIANATABACUM 286 Met Glu Thr ValGlu Met Ile Val Lys Val Ser Cys Ala Ala Ile Val 1 5 10 15 Ile Thr LeuLeu Val Cys Leu Trp Arg Val Leu Asn Trp Val Trp Phe 20 25 30 Arg Pro LysLys Leu Glu Lys Leu Leu Arg Lys Gln Val Leu Tyr Gly 35 40 45 Asp Met LysGlu Phe Ser Gly Met Ile Lys Glu Ala Tyr Ser Lys Pro 50 55 60 Met Ser LeuSer Asp Asp Val Ala Pro Arg Met Met Pro Phe Phe Leu 65 70 75 80 Glu ThrIle Lys Lys Tyr Gly Lys Arg Ser Phe Ile Trp Phe Gly Pro 85 90 95 Arg ProLeu Val Leu Ile Met Asp Pro Glu Leu Ile Lys Glu Val Leu 100 105 110 SerLys Ile Tyr Leu Tyr Gln Lys Pro Gly Gly Asn Pro Leu Ala Thr 115 120 125Leu Leu Val Gln Gly Leu Ala Thr Tyr Glu Glu Asp Lys Trp Ala Lys 130 135140 His Arg Lys Ile Ile Asn Pro Ala Phe His Leu Glu Lys Leu Lys His 145150 155 160 Met Leu Pro Ala Phe Arg Leu Ser Cys Ser Glu Met Leu Ser LysTrp 165 170 175 Glu Asp Ile Val Ser Ala Glu Gly Ser His Glu Ile Asp ValTrp Pro 180 185 190 Asn Leu Glu Gln Leu Ser Cys Asp Val Ile Ser Arg ThrAla Phe Gly 195 200 205 Asn Ser Tyr Glu Glu Gly Arg Lys Ile Phe Glu LeuGln Lys Glu Gln 210 215 220 Thr Gln His Leu Val Glu Ala Phe Arg Ser ValTyr Ile Pro Gly Arg 225 230 235 240 Arg Phe Leu Pro Thr Lys Arg Asn ArgArg Met Lys Glu Ile Lys Lys 245 250 255 Glu Val Arg Ala Ser Ile Lys GlyIle Ile Asp Lys Arg Leu Lys Ala 260 265 270 Met Lys Ala Gly Asp Thr AsnAsn Glu Asp Leu Leu Gly Tyr Cys Trp 275 280 285 Asn Gln Ile Leu Lys LysLeu Asn Ser Ala Glu Thr Arg Ile Leu Glu 290 295 300 287 1559 DNANICOTIANATABACUM 287 ccaaaatgca gttcttcaac ttcatttcct ttgtcttttttgtgtctttc ctctttttat 60 taaggaaatg gaagaactcc aatagccaaa ccaaaagattgcctccaggt ccatggaaat 120 tacctgtact tggaagcatg tttcatttgc taggtggacctccacatcat gtccttggag 180 atttagccaa aaaatatggt ccacttatgc accttcaactaggtgaagtt tctgtagttt 240 ctgttacttc tcctgagatg gcaaaagaag tactaaaaactcatgacctc gcttttgcat 300 ctaggccgtt acttttggca gccaaaattg tctgctataatgggacagac attgtctttt 360 ccccctatgg cgattattgg agacaaacgc gtaaaatttgtctcttggaa ttgctcagtg 420 ccaaaaatgt taggtcattc agctcagtca gacgagatgaagttttccat atgattgaat 480 ttttttcgat catcttctgg taagccagtt aatgtatcaaaaaggatttc tctattcaca 540 acctctatga catgtagatc agcctttgga caagaatacaaggagcaaga cgaattcgca 600 caactagtaa aaaaagtgtc aagcttaatg gaagggtttgatgttgctga tatattccct 660 tcattgaagt ttcttcatgt gctcagtgga atgaaggctaaagttatgga tgcacaccat 720 gagttagatg ccattcttga aaaaattatc aatgagcacaagaaaattgc aactggaaag 780 aataataatg aattaggagg tgaaggatta attgacgtactgctaagact tatgaaagag 840 ggaggccttc aattcccgat caccaacgac aacatcaaagctattatttt tgacatgttt 900 ggtgcgggaa cggaaacttc atcaaccaca attgactgggccatggtcga aatgataaag 960 aatccaagtg tattcgctaa agctcaagca gaggtaagagaagccttcag agagaaagaa 1020 acttttgatg aaaatgatgt cgaggagttg aaatacttaaaattggttat caaagaaact 1080 ttcagactcc atcctccatt tccccttttg ctcccaagagaatctagaga agaaacagat 1140 ataaacggct acactattcc ttttaaaaca aaacttatggttaacgttcg ggctattgga 1200 agagatccaa aatattggga tgacgtggaa agttttaagccagagagatt tgagcacaac 1260 tctatggatt ttattggtaa taattttgaa tatcttccctttggtagtgg aaggagaatg 1320 tgccctggga tatcatttgg tttggctaat gtttatttgccactagctca attgttatat 1380 cattttgatt ggaaactccc tactggaatc aattcaagtgacttggacat gactgagtcg 1440 tcaggagtaa cttgtgctag aaagagtgat ttatacttgactgctactcc atatcaactt 1500 tctcaagagt gatgcaatga tatcaacctt ttgaatttcggtcaacccca ccaatagtg 1559 288 165 PRT NICOTIANATABACUM 288 Met Gln PhePhe Asn Phe Ile Ser Phe Val Phe Phe Val Ser Phe Leu 1 5 10 15 Phe LeuLeu Arg Lys Trp Lys Asn Ser Asn Ser Gln Thr Lys Arg Leu 20 25 30 Pro ProGly Pro Trp Lys Leu Pro Val Leu Gly Ser Met Phe His Leu 35 40 45 Leu GlyGly Pro Pro His His Val Leu Gly Asp Leu Ala Lys Lys Tyr 50 55 60 Gly ProLeu Met His Leu Gln Leu Gly Glu Val Ser Val Val Ser Val 65 70 75 80 ThrSer Pro Glu Met Ala Lys Glu Val Leu Lys Thr His Asp Leu Ala 85 90 95 PheAla Ser Arg Pro Leu Leu Leu Ala Ala Lys Ile Val Cys Tyr Asn 100 105 110Gly Thr Asp Ile Val Phe Ser Pro Tyr Gly Asp Tyr Trp Arg Gln Thr 115 120125 Arg Lys Ile Cys Leu Leu Glu Leu Leu Ser Ala Lys Asn Val Arg Ser 130135 140 Phe Ser Ser Val Arg Arg Asp Glu Val Phe His Met Ile Glu Phe Phe145 150 155 160 Ser Ile Ile Phe Trp 165 289 1592 DNA NICOTIANATABACUM289 ccccaccaaa aaatcatttc tctcgtctaa aatggatctt ctcttactag agaagacctt 60aattggtctt ttctttgcca ttttaatcgc tttaattgtc tctaaacttc gttcaaagcg 120ttttaagctt cctccaggac caattccagt accagttttt ggtaattggc ttcaagttgg 180tgatgattta aaccacagaa atcttactga ttatgccaag aaatttggag atcttttctt 240gttaagaatg ggtcaacgta acttagttgt tgtgtcatct cctgaattag ctaaagaagt 300tttacacaca caaggtgttg aatttggttc aagaacaaga aatgttgtgt ttgatatttt 360tactggaaaa ggtcaagata tggtttttac tgtatatggt gaacattgga gaaaaatgag 420gagaattatg actgtaccat tttttactaa taaagttgtg caacagtata gaggggggtg 480ggagtttgag gtggcaagtg taattgagga tgtgaaaaaa aatcctgaat ctgctactaa 540tgggatcgta ttaaggagga gattacaatt aatgatgtat aataatatgt ttaggattat 600gtttgatagg agatttgaga gtgaagatga tcctttgttt gttaagctta aggctttgaa 660tggtgaaagg agtagattgg ctcaaagttt tgagtataat tatggtgatt ttattccaat 720tttgaggcct ttttttgaga ggttatttga agatctgtaa agaagttaag gagaagaggc 780tgcagctttt caaagattac tttgttgatg aaagaaagaa gctttcgaat accaagagct 840cggacagcaa tgccctaaaa tgtgcgattg atcacattct tgaggctcaa cagaagggag 900agatcaatga ggacaacgtt ctttacattg ttgaaaacat caatgttgct gcaattgaaa 960caacattatg gtcaattgag tggggtatcg ccgagctagt caaccaccct cacatccaaa 1020agaaactgcg cgacgagatt gacacagttc ttggaccagg agtgcaagtg actgaaccag 1080acacccacaa gcttccatac cttcaggctg tgatcaagga ggcacttcgt ctccgtatgg 1140caattcctct attagtccca cacatgaacc ttcacgacgc aaagcttggc gggtttgata 1200ttccagcaga gagcaaaatc ttggttaacg cttggtggtt agctaacaac ccggctcatt 1260ggaagaaacc cgaagagttc agacccgaga ggttctttga agaggagaag catgttgagc 1320caatggcaat gacttcagat atcttccgtt tggcgttggt aggaggagct gccctggaat 1380tatacttgca ttgccaactc ttggcatcac tttgggacgt ttggttcaga atgttgcctc 1440ctccaggcca gtcgaagctc gacaccacag agaaaggtgg acagttcagt ctccacattt 1500tgaagcattc caccattgtg ttgaaaccaa ggtctttctg aactttgtga tcttattaat 1560taaggggttc tgaagaaatt tgatagtgtt gg 1592 290 242 PRT NICOTIANATABACUM290 Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 510 15 Ile Leu Ile Ala Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys 2025 30 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 3540 45 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys 5055 60 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 6570 75 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val85 90 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp ArgLys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys ValVal Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser ValIle Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn GlyIle Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn MetPhe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp ProLeu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg LeuAla Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile LeuArg Pro Phe Phe Glu Arg Leu Phe Glu 225 230 235 240 Asp Leu 291 1544 DNANICOTIANATABACUM 291 ataatgctct ttctactctt tgtagccctt cctttcattcttatttttct tcttcctaaa 60 ttcaaaaatg gtggaaataa cagattgcca ccaggtcctataggtttacc attcattgga 120 aatttgcatc aatatgatag tataactcct catatctatttttggaaact ttccaaaaaa 180 tatggcaaaa tcttctcatt aaaacttgct tctactaatgtggtagtagt ttcttcagca 240 aaattagcaa aagaagtatt gaaaaaacaa gatttaatattttgtagtag accatctatt 300 cttggccaac aaaaactgtc ttattatggt cgtgatattgcttttgcacc ttataatgat 360 tattggagag aaatgagaaa aatttgtgtt cttcatctttttagtttaaa aaaagttcaa 420 ttatttagtc caattcgtga agatgaagtt tttagaatgattaagaaaat atcaaaacaa 480 gcttctactt cacaaattat taatttgagt aatttaatgatttcattaac aagtacaatt 540 atttgtagag ttgcttttgg tgttaggttt gaagaagaagcacatgcaag gaagagattt 600 gattttcttt tggccgaggc acaagaaatg atggctagtttctttgtatc tgattttttt 660 ccctttttaa gttagattga caaattaagt ggattgacatatagacttga gaggaatttc 720 aaggatttgg ataattttta tgaagaactc attgagcaacatcaaaatcc taataagcca 780 aaatatatgg aaggagatat tgttgatctt ttgctacaattgaagaaaga gaaattaaca 840 ccacttgatc tcactatgga agatataaaa ggaattctcatgaatgtgtt agttgcagga 900 tcagacacta gtgcagctgc tactgtttgg gcaatgacagccttgataaa gaatcctaaa 960 gccatggaaa aagttcaatt agaaatcaga aaatcagttgggaagaaagg cattgtaaat 1020 gaagaagatg tccaaaacat cccttatttt aaagcagtgataaaggaaat atttagattg 1080 tatccaccag ctccactttt agttccaaga gaatcaatggaaaaaaccat attagaaggt 1140 tatgaaattc ggccaagaac catagttcat gttaacgcttgggctatagc aagggatcct 1200 gaaatatggg aaaatccaga tgaatttata cctgagagatttttgaatag cagtatcgat 1260 tacaagggtc aagattttga gttacttcca tttggtgcaggcagaagagg ttgcccaggt 1320 attgcacttg gggttgcatc catggaactt gctttgtcaaatcttcttta tgcatttgat 1380 tgggagttgc cttatggagt gaaaaaagaa gacatcgacacaaacgttag gcctggaatt 1440 gccatgcaca agaaaaacga actttgcctt gtcccaaaaaaattatttat aaattatatt 1500 gggacgtgga tctcatgcta gttctgtgcg gtcagctaagctta 1544 292 501 PRT NICOTIANATABACUM 292 Met Leu Phe Leu Leu Phe ValAla Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys AsnGly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe IleGly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe TrpLys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala SerThr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu ValLeu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu GlyGln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala ProTyr Trp Arg Glu Met Arg Lys Ile Cys Val Leu His 115 120 125 Leu Phe SerLeu Lys Lys Val Gln Leu Phe Ser Pro Ile Arg Glu Asp 130 135 140 Glu ValPhe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala Ser Thr Ser 145 150 155 160Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr Ser Thr Ile 165 170175 Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu Ala His Ala 180185 190 Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu Met Met Ala195 200 205 Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Ile Asp LysLeu 210 215 220 Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys Asp LeuAsp Asn 225 230 235 240 Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn ProAsn Lys Pro Lys 245 250 255 Tyr Met Glu Gly Asp Ile Val Asp Leu Leu LeuGln Leu Lys Lys Glu 260 265 270 Lys Leu Thr Pro Leu Asp Leu Thr Met GluAsp Ile Lys Gly Ile Leu 275 280 285 Met Asn Val Leu Val Ala Gly Ser AspThr Ser Ala Ala Ala Thr Val 290 295 300 Trp Ala Met Thr Ala Leu Ile LysAsn Pro Lys Ala Met Glu Lys Val 305 310 315 320 Gln Leu Glu Ile Arg LysSer Val Gly Lys Lys Gly Ile Val Asn Glu 325 330 335 Glu Asp Val Gln AsnIle Pro Tyr Phe Lys Ala Val Ile Lys Glu Ile 340 345 350 Phe Arg Leu TyrPro Pro Ala Pro Leu Leu Val Pro Arg Glu Ser Met 355 360 365 Glu Lys ThrIle Leu Glu Gly Tyr Glu Ile Arg Pro Arg Thr Ile Val 370 375 380 His ValAsn Ala Trp Ala Ile Ala Arg Asp Pro Glu Ile Trp Glu Asn 385 390 395 400Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser Ser Ile Asp Tyr 405 410415 Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Gly 420425 430 Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu Ser435 440 445 Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val LysLys 450 455 460 Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala Met HisLys Lys 465 470 475 480 Asn Glu Leu Cys Leu Val Pro Lys Lys Leu Phe IleAsn Tyr Ile Gly 485 490 495 Thr Trp Ile Ser Cys 500 293 1764 DNANICOTIANATABACUM 293 gtgaggtttg aatcctctgc ctcaatgaaa ctcaccaaattggttttcta atttccatct 60 aaaatattgt ccaaagctaa agattctttc tccttaaatagtcaacttta gtggttcctc 120 ttcatttcat agctcaatct ttcttatttt gattcaaccatggagaacca atactcctac 180 tcattctctt cctacttcta cttagctata gtactgtttcttcttccaat tttggtcaaa 240 tatttcttcc atcggagaag aaatttacct ccaagtccattttctcttcc aataattggt 300 cacctttacc ttctcaagaa aactctccat ctcactctaacatccttatc agctaaatat 360 ggtcctgttt tatacctcaa attgggctct atgcctgtgattgttgtgtc ctcaccatct 420 gctgttgaag aatgtttaac caagaatgat atcatattcgcaaataggcc caagaccgtg 480 gctggtgaca agtttaccta caattatact gtttatgtttgggcacccta tggccaactt 540 tggagaattc ttcgccgatt aactgtcgtt gaactcttctcttcacatag cctacagaaa 600 acttctatcc ttagagatca agaagttgca atatttatccgttcgttata caaattctca 660 aaggatagta gcaaaaaagt cgatttgacc aactggtcttttactttggt tttcaatctt 720 atgaccaaaa ttattgctgg gagacatatt gtgaaggaggaagatgctgg caaggaaaag 780 ggcattgaaa ttattgaaaa acttagaggg actttcttagtaactacatc attcttgaat 840 atgtgtgatt tcttgccagt attcaggtgg gttggttacaaagggctgga gaagaagatg 900 gcctcaattc acaatagaag aaatgaattc ttgaacagcttgcttgatga atttcgacac 960 aagaaaagta gtgcttcaca atctaacaca actgttggaaacatggagaa gaaaaccaca 1020 ctgattgaaa agctcttgtc tcttcaagaa tcagagcctgaattctacac tgatgatata 1080 tcaaaagtat tatgctggta gtttttgttg caggaacagagacctcatca acaaccatcc 1140 aatgggtaat gaggcttctt gtagctcacc ctgaggcattgtataagcta cgagctgaca 1200 ttgacagtaa agttgggaat aagcgcttgc tgaatgaatcagacctcaac aagcttccgt 1260 atttgcattg tgttgttaat gagacaatga gattatacactccgatacca cttttattgc 1320 ctcattattc aactaaagat tgtattgtgg aaggatatgatgtaccaaaa catacaatgt 1380 tgtttgtcaa cgcttgggcc attcacaggg atcccaaggtatgggaggag cctgacaagt 1440 tcaagccaga gagatttgag gcaacagaag gggaaacagaaaggttcaat tacaagcttg 1500 taccatttgg aatggggaga agagcgtgcc ctggagctgatatggggttg cgagcagttt 1560 ctttggcatt aggtgcactt attcaatgct ttgactggcaaattgaggaa gcggaaagct 1620 tggaggaaag ctataattct agaatgacta tgcagaacaagcctttgaag gttgtctgca 1680 ctccacgcga agatcttggc cagcttctat cccaactctaaggcaattta tcaatgccaa 1740 acgtaatctt catctaccac tatg 1764 294 520 PRTNICOTIANATABACUM 294 Met Glu Asn Gln Tyr Ser Tyr Ser Phe Ser Ser Tyr PheTyr Leu Ala 1 5 10 15 Ile Val Leu Phe Leu Leu Pro Ile Leu Val Lys TyrPhe Phe His Arg 20 25 30 Arg Arg Asn Leu Pro Pro Ser Pro Phe Ser Leu ProIle Ile Gly His 35 40 45 Leu Tyr Leu Leu Lys Lys Thr Leu His Leu Thr LeuThr Ser Leu Ser 50 55 60 Ala Lys Tyr Gly Pro Val Leu Tyr Leu Lys Leu GlySer Met Pro Val 65 70 75 80 Ile Val Val Ser Ser Pro Ser Ala Val Glu GluCys Leu Thr Lys Asn 85 90 95 Asp Ile Ile Phe Ala Asn Arg Pro Lys Thr ValAla Gly Asp Lys Phe 100 105 110 Thr Tyr Asn Tyr Thr Val Tyr Val Trp AlaPro Tyr Gly Gln Leu Trp 115 120 125 Arg Ile Leu Arg Arg Leu Thr Val ValGlu Leu Phe Ser Ser His Ser 130 135 140 Leu Gln Lys Thr Ser Ile Leu ArgAsp Gln Glu Val Ala Ile Phe Ile 145 150 155 160 Arg Ser Leu Tyr Lys PheSer Lys Asp Ser Ser Lys Lys Val Asp Leu 165 170 175 Thr Asn Trp Ser PheThr Leu Val Phe Asn Leu Met Thr Lys Ile Ile 180 185 190 Ala Gly Arg HisIle Val Lys Glu Glu Asp Ala Gly Lys Glu Lys Gly 195 200 205 Ile Glu IleIle Glu Lys Leu Arg Gly Thr Phe Leu Val Thr Thr Ser 210 215 220 Phe LeuAsn Met Cys Asp Phe Leu Pro Val Phe Arg Trp Val Gly Tyr 225 230 235 240Lys Gly Leu Glu Lys Lys Met Ala Ser Ile His Asn Arg Arg Asn Glu 245 250255 Phe Leu Asn Ser Leu Leu Asp Glu Phe Arg His Lys Lys Ser Ser Ala 260265 270 Ser Gln Ser Asn Thr Thr Val Gly Asn Met Glu Lys Lys Thr Thr Leu275 280 285 Ile Glu Lys Leu Leu Ser Leu Gln Glu Ser Glu Pro Glu Phe TyrThr 290 295 300 Asp Asp Ile Ile Lys Ser Ile Met Leu Val Val Phe Val AlaGly Thr 305 310 315 320 Glu Thr Ser Ser Thr Thr Ile Gln Trp Val Met ArgLeu Leu Val Ala 325 330 335 His Pro Glu Ala Leu Tyr Lys Leu Arg Ala AspIle Asp Ser Lys Val 340 345 350 Gly Asn Lys Arg Leu Leu Asn Glu Ser AspLeu Asn Lys Leu Pro Tyr 355 360 365 Leu His Cys Val Val Asn Glu Thr MetArg Leu Tyr Thr Pro Ile Pro 370 375 380 Leu Leu Leu Pro His Tyr Ser ThrLys Asp Cys Ile Val Glu Gly Tyr 385 390 395 400 Asp Val Pro Lys His ThrMet Leu Phe Val Asn Ala Trp Ala Ile His 405 410 415 Arg Asp Pro Lys ValTrp Glu Glu Pro Asp Lys Phe Lys Pro Glu Arg 420 425 430 Phe Glu Ala ThrGlu Gly Glu Thr Glu Arg Phe Asn Tyr Lys Leu Val 435 440 445 Pro Phe GlyMet Gly Arg Arg Ala Cys Pro Gly Ala Asp Met Gly Leu 450 455 460 Arg AlaVal Ser Leu Ala Leu Gly Ala Leu Ile Gln Cys Phe Asp Trp 465 470 475 480Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser Tyr Asn Ser Arg Met 485 490495 Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys Thr Pro Arg Glu Asp 500505 510 Leu Gly Gln Leu Leu Ser Gln Leu 515 520 295 1694 DNANICOTIANATABACUM 295 aacatccttt ccttcttcca aaaatggagc ttcaatcttctcctttcaat ttaatttctt 60 tgttcctctt cttttctttt ctttttattc tagtgaagaaatggaatgcc aaaatcccaa 120 agttacctcc aggtccgtgg aggcttccct ttattggaagcctccatcac ttgaagggaa 180 aacttccaca ccataatctt agagatctag cgcgaaaatatggacctctc atgtacttac 240 aactcggaga aattcctgta gttgtaatat cttcgccacgtgtagcaaaa gctgtactaa 300 aaactcatga tctcgctttt gcaactagac cacgattcatgtcctcagac attgtgtttt 360 acaaaagcag ggacatctct tttgccccat ttggtgattactggagacag atgcgtaaaa 420 tattgactca ggaactcctg agcaacaaga tgctcaagtcatatagctta atccgaaagg 480 atgagctctc gaagctcctc tcatcgattc gtttggaaacaggttctgca gtgaacataa 540 atgaaaagct tctctggttt acgagctgca tgacctgtagattagccttt ggaaaaatat 600 gcaatgatcg ggatgagttg atcatgctaa ttagggagatattaacatta tcaggaggat 660 ttgatgtggg tgatttgttc ccttcctgga aattacttcataatatgagc aacatgaaag 720 ctaggttgac gaatgtacac cacaagtatg atttagttatggagaacatc atcaatgagc 780 accaagagaa tcatgcagca gggataaagg gtaacaacgagtttggtggc gaagatatga 840 tcgatgctct actgagggct aaggagaata atgagcttcaatttcctatc gaaaatgaca 900 acatgaaagc agtaattctg gacttgttta ttgctggaactgaaacttca tatactgcaa 960 ttatatgggc actatcagaa ttgatgaagc acccaagtgtgatggccaag gcacaagctg 1020 aagtgagaaa agtcttcaaa gaaaatgaaa atttcgacgaaaatgatctt gacaagttgc 1080 catacctaaa atcagtgatt aaagaaacac taaggatgcaccctccagtt cctttgttag 1140 ggcctagaga atgcagggac caaacagaga tcgatggctacactgtacct attaaagcta 1200 gagttatggt taatgcttgg gcgataggaa gagatcctgaaagttgggaa gatcctgaaa 1260 gtttcaaacc ggagcgattt gaaaatactt ctgttgatcttacaggaaat cactatcagt 1320 tcattccttt cggttcagga agaagaatgt gtccaggaatgtcgtttggt ttagttaaca 1380 cagggcatcc tttagcccag ttgctctatt gctttgactggaaactccct gacaaggtta 1440 atgcaaatga ttttcgcact actgaaacaa gtagagtttttgcagcaagc aaagatgacc 1500 tctacttgat tcccacaaat cacagggagc aagaatagcttaatttaatg gagttcttgg 1560 aagaattaaa gaagaagggc tatataggtg agattttttgtatggttgca aggtttttag 1620 ttcatacaat aagacaatac attatattcc agtattgtgtatcatgtata ataaggttcc 1680 ttttgtttaa aaaa 1694 296 504 PRTNICOTIANATABACUM 296 Met Glu Leu Gln Ser Ser Pro Phe Asn Leu Ile Ser LeuPhe Leu Phe 1 5 10 15 Phe Ser Phe Leu Phe Ile Leu Val Lys Lys Trp AsnAla Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Phe IleGly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His Asn Leu ArgAsp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly GluIle Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Val Ala Lys Ala ValLeu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met SerSer Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala ProPhe Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln GluLeu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Tyr Ser Leu Ile Arg LysAsp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Glu ThrGly Ser Ala Val Asn Ile Asn Glu Lys Leu 165 170 175 Leu Trp Phe Thr SerCys Met Thr Cys Arg Leu Ala Phe Gly Lys Ile 180 185 190 Cys Asn Asp ArgAsp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu Thr 195 200 205 Leu Ser GlyGly Phe Asp Val Gly Asp Leu Phe Pro Ser Trp Lys Leu 210 215 220 Leu HisAsn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His His 225 230 235 240Lys Tyr Asp Leu Val Met Glu Asn Ile Ile Asn Glu His Gln Glu Asn 245 250255 His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp Met 260265 270 Ile Asp Ala Leu Leu Arg Ala Lys Glu Asn Asn Glu Leu Gln Phe Pro275 280 285 Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe IleAla 290 295 300 Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu SerGlu Leu 305 310 315 320 Met Lys His Pro Ser Val Met Ala Lys Ala Gln AlaGlu Val Arg Lys 325 330 335 Val Phe Lys Glu Asn Glu Asn Phe Asp Glu AsnAsp Leu Asp Lys Leu 340 345 350 Pro Tyr Leu Lys Ser Val Ile Lys Glu ThrLeu Arg Met His Pro Pro 355 360 365 Val Pro Leu Leu Gly Pro Arg Glu CysArg Asp Gln Thr Glu Ile Asp 370 375 380 Gly Tyr Thr Val Pro Ile Lys AlaArg Val Met Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro GluSer Trp Glu Asp Pro Glu Ser Phe Lys Pro 405 410 415 Glu Arg Phe Glu AsnThr Ser Val Asp Leu Thr Gly Asn His Tyr Gln 420 425 430 Phe Ile Pro PheGly Ser Gly Arg Arg Met Cys Pro Gly Met Ser Phe 435 440 445 Gly Leu ValAsn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Cys Phe 450 455 460 Asp TrpLys Leu Pro Asp Lys Val Asn Ala Asn Asp Phe Arg Thr Thr 465 470 475 480Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu Ile 485 490495 Pro Thr Asn His Arg Glu Gln Glu 500 297 1819 DNA NICOTIANATABACUM297 caatcagtgg atgcgggagt aatatataat atgcaagttg tagaaagaga aaaaaaaaat 60caagtagcta ttctatactg gggcacaaat agtgagtgaa aatggagact gttcaaatca 120taataacagc atcttgtgct gccataataa ttactctagt ggtgtgtatt tggagagtac 180tgaattgggt ttggttcaga ccaaagaagc tggaaaaact attgaggaaa caaggtctca 240aaggcaactc ctacaagatt ttgtatgggg atatgaagga gctttctggt atgattaagg 300aagctaattc caaacccatg aatctttctg atgatattgc accaagattg gtgcctttct 360ttcttgacac catcaagaaa tatggtaaaa aatcctttgt atggttaggt ccgaaaccac 420tggttcttat catggaccct gagcttataa aggaaatatt ttccaaatac tatctgtatc 480aaaagcctca tggaaatcca gttaccaagc tattagtaca aggactagta agcctagagg 540aagacaaatg ggccaaacat agaaaaatca tcaatccagc tttccatcta gagaagctaa 600agcatatgct tccagctttt tgcttgagct gcactgagat gctgtgcaaa tgggaagata 660ttgtttcaat taagggctca catgagatag atgtatggcc tcaccttgaa caattaagta 720gcgatgtgat ctctcggaca gcttttggca gtaactttga agaaggtaaa aggatatttg 780aacttcagaa ggaacaagct cagtattttg tagaagctat acgctcggtt tatataccag 840gctggaggtt tttgccaaca aagaggaaca gaagaatgaa ggaagttgaa aaggatgttc 900gggcctcgat aagaggcatt attgataaaa gagtgaaggc aatgaaagca ggagaggcga 960gtaatgagga tctacttggt atattgttgg aatctaattt tacagaagct gaacagcata 1020gacacaagga ttctgcgatg agcattgaag aagtcattca agaatgcaag ttattctatg 1080ttgctggcca agaaactaca tcagtgttgc ttgtgtggac tctaatattg ttgagtaggc 1140atcaagattg gcagagccga gccagagaag aggtgtttca agtctttggt aatcagaaac 1200cagattttga cggattgaat cgtctaaaag ttgtgacaat gatcttgtat gagtctttaa 1260ggctatactc cccagtagtg tcactaatcc ggcggcctaa tgaggatgct atattaggaa 1320atgtatctct gccagaaggt gtgctactct cattaccagt gatcttatta caccacgatg 1380aagagatatg gggtaaagat gcaaagaagt tcaatccaga aagatttaga gatggagtct 1440caagtgcaac aaagggtcaa gtcacttttt ttccatttac ttggggtccc agaatatgca 1500tcggacaaaa ttttgccatg ttagaagcaa agactgcttt ggctatgatc ctacaacgct 1560tctcattcga actgtctcca tcttatgcac atgctcctca gtccatatta actatgcaac 1620cccaacatgg tgctccacta attctgcaca aaatatagtt tgttacttta agcagtgtct 1680tgttatatgt cagagagtcc aaaatgttta attaaggctt gtagaactgc caaatggaac 1740ttcatttgca ttcgtgggtt gtagattgtt gtaattggac aagtatactg tttattttag 1800agttttaaga aaaaaaaaa 1819 298 518 PRT NICOTIANATABACUM 298 Met Glu ThrVal Gln Ile Ile Ile Thr Ala Ser Cys Ala Ala Ile Ile 1 5 10 15 Ile ThrLeu Val Val Cys Ile Trp Arg Val Leu Asn Trp Val Trp Phe 20 25 30 Arg ProLys Lys Leu Glu Lys Leu Leu Arg Lys Gln Gly Leu Lys Gly 35 40 45 Asn SerTyr Lys Ile Leu Tyr Gly Asp Met Lys Glu Leu Ser Gly Met 50 55 60 Ile LysGlu Ala Asn Ser Lys Pro Met Asn Leu Ser Asp Asp Ile Ala 65 70 75 80 ProArg Leu Val Pro Phe Phe Leu Asp Thr Ile Lys Lys Tyr Gly Lys 85 90 95 LysSer Phe Val Trp Leu Gly Pro Lys Pro Leu Val Leu Ile Met Asp 100 105 110Pro Glu Leu Ile Lys Glu Ile Phe Ser Lys Tyr Tyr Leu Tyr Gln Lys 115 120125 Pro His Gly Asn Pro Val Thr Lys Leu Leu Val Gln Gly Leu Val Ser 130135 140 Leu Glu Glu Asp Lys Trp Ala Lys His Arg Lys Ile Ile Asn Pro Ala145 150 155 160 Phe His Leu Glu Lys Leu Lys His Met Leu Pro Ala Phe CysLeu Ser 165 170 175 Cys Thr Glu Met Leu Cys Lys Trp Glu Asp Ile Val SerIle Lys Gly 180 185 190 Ser His Glu Ile Asp Val Trp Pro His Leu Glu GlnLeu Ser Ser Asp 195 200 205 Val Ile Ser Arg Thr Ala Phe Gly Ser Asn PheGlu Glu Gly Lys Arg 210 215 220 Ile Phe Glu Leu Gln Lys Glu Gln Ala GlnTyr Phe Val Glu Ala Ile 225 230 235 240 Arg Ser Val Tyr Ile Pro Gly TrpArg Phe Leu Pro Thr Lys Arg Asn 245 250 255 Arg Arg Met Lys Glu Val GluLys Asp Val Arg Ala Ser Ile Arg Gly 260 265 270 Ile Ile Asp Lys Arg ValLys Ala Met Lys Ala Gly Glu Ala Ser Asn 275 280 285 Glu Asp Leu Leu GlyIle Leu Leu Glu Ser Asn Phe Thr Glu Ala Glu 290 295 300 Gln His Arg HisLys Asp Ser Ala Met Ser Ile Glu Glu Val Ile Gln 305 310 315 320 Glu CysLys Leu Phe Tyr Val Ala Gly Gln Glu Thr Thr Ser Val Leu 325 330 335 LeuVal Trp Thr Leu Ile Leu Leu Ser Arg His Gln Asp Trp Gln Ser 340 345 350Arg Ala Arg Glu Glu Val Phe Gln Val Phe Gly Asn Gln Lys Pro Asp 355 360365 Phe Asp Gly Leu Asn Arg Leu Lys Val Val Thr Met Ile Leu Tyr Glu 370375 380 Ser Leu Arg Leu Tyr Ser Pro Val Val Ser Leu Ile Arg Arg Pro Asn385 390 395 400 Glu Asp Ala Ile Leu Gly Asn Val Ser Leu Pro Glu Gly ValLeu Leu 405 410 415 Ser Leu Pro Val Ile Leu Leu His His Asp Glu Glu IleTrp Gly Lys 420 425 430 Asp Ala Lys Lys Phe Asn Pro Glu Arg Phe Arg AspGly Val Ser Ser 435 440 445 Ala Thr Lys Gly Gln Val Thr Phe Phe Pro PheThr Trp Gly Pro Arg 450 455 460 Ile Cys Ile Gly Gln Asn Phe Ala Met LeuGlu Ala Lys Thr Ala Leu 465 470 475 480 Ala Met Ile Leu Gln Arg Phe SerPhe Glu Leu Ser Pro Ser Tyr Ala 485 490 495 His Ala Pro Gln Ser Ile LeuThr Met Gln Pro Gln His Gly Ala Pro 500 505 510 Leu Ile Leu His Lys Ile515

What is claimed is:
 1. An isolated nucleic acid molecule from Nicotiana,wherein said nucleic acid molecule comprises a nucleic acid sequenceselected from the group consisting of SEQ. ID. No.:149, 151, 153, 155,157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183,185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211,213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239,241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267,269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and297.
 2. An isolated nucleic acid molecule from Nicotiana wherein saidnucleic acid molecule comprising a nucleic acid sequence selected fromthe group consisting of SEQ. ID. No. 299 through SEQ. ID. No.
 357. 3. Anisolated protein from Nicotiana, wherein said protein comprises an aminoacid sequence selected from the group consisting of SEQ. ID. No.:. 150,152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178,180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206,208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234,236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262,264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290,292, 294, 296 and
 298. 4. A transgenic plant, wherein said transgenicplant comprises the nucleic acid molecule of claim 1 or
 2. 5. Thetransgenic plant of claim 4, wherein said plant is a tobacco plant.
 6. Amethod of producing a transgenic plant, wherein said method comprisesthe steps of: (i) operably linking said nucleic acid molecule of any oneof the claims 1 or 2 with a promoter functional in said plant to createa plant transformational vector; (ii) transforming said plant with saidplant transformational vector of step; (iii) selecting a plant celltransformed with said transformation vector; and (iv) regenerating atransformation plant from said transformed plant cell.
 7. The method ofclaim 6, wherein said nucleic acid molecule is in an antisenseorientation.
 8. The method of claim 6, wherein said nucleic acidmolecule is in a sense orientation.
 9. The method of claim 6, whereinsaid nucleic acid molecule is in a RNA interference orientation.
 10. Themethod of claim 6, wherein said nucleic acid molecule is expressed as adouble stranded RNA molecule.
 11. The method of claim 6, wherein saiddouble stranded RNA molecule is about 15 to 25 nucleotides in length.12. The method of claim 6, wherein said transgenic plant is a tobaccoplant.
 13. A method of selecting a plant containing a nucleic acidmolecule, wherein said plant is analyzed for the presence of nucleicacid sequence selected from the group consisting of 149, 151, 153, 155,157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183,185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211,213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239,241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267,269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and297.
 14. The method of selecting a plant of claim 13, wherein said plantis analyzed by DNA hybridization.
 15. The method of selecting a plant ofclaim 13, wherein said DNA hybridization is Southern blot analysis. 16.The method of selecting a plant of claim 13, wherein said DNAhybridization is Northern blot analysis.
 17. The method of selecting aplant of claim 13, wherein said plant is analyzed by PCR detection. 18.The method of claim 13, wherein said plant is a tobacco plant.